| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,962,363 | T→C | 43.8% | E331E (GAA→GAG) | yjjI ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,962,363 | 0 | T | C | 43.8% | 19.5 / 70.4 | 89 | E331E (GAA→GAG) | yjjI | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (25/25); new base C (17/22); total (42/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.69e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.65e-01 | |||||||||||
CTGATGCGATAACCTACTTCATTTGCGGCGGCTTCTTTACCGTAGCGCGCGGCAATCCCTTCTTTTTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATACATGCCAAACATTGGCACAAA > minE/2962298‑2962419 | cTGATGCGATAACCTACTTCATTTGCGGCGGCTTCTTTACCGTAGCGCGCGGCAATCCCTTCTTTTTca < 1:920902/69‑1 (MQ=255)cTGATGCGATAACCTACTTCATTTGCGGCGGCTTCTTTACCGTAGCGCGCGGCAATCCCTTCTTTTTca < 1:2706501/69‑1 (MQ=255)cTGATGCGATAACCTACTTCATTTGCGGCGGCTTCTTTACCGTAGCGCGCGGCAATCCCTTCTTTTTca < 1:2745754/69‑1 (MQ=255)cTGATGCGATAACCTACTTCATTTGCGGCGGCTTCTTTACCGTAGCGCGCGGCAATCCCTTCTTTTTca < 1:1736066/69‑1 (MQ=255)cTGATGCGATAACCTACTTCATTTGCGGCGGCTTCTTTACCGTAGCGCGCGGCAATCCCTTCTTTTTca < 1:3385725/69‑1 (MQ=255) cTTCATTTGCGGCGGCTTCTTTACCGTAGCGCGCGTCAATCCCTTCTTTTTCACACAGCAAGTTAACCg > 1:2150663/1‑69 (MQ=255) cTTCATTTGCGGCGGCTTCTTTACCGTAGCGCGCGTCAATCCCTTCTTTTTCACACAGCAAGTTAACCg > 1:1928964/1‑69 (MQ=255) cTTCATTTGCGGCGGCTTCTTTACCGTAGCGCGCGTCAATCCCTTCTTTTTCACACAGCAAGTTAACCg > 1:2217331/1‑69 (MQ=255) cTTCATTTGCGGAGGCTTCTTTACCGTAGCGCGCGTCAATCCCTTCTTTTTCACACAGCAAGTTAACCg > 1:1112162/1‑69 (MQ=255) tCATTTGCGGCGGCTTCTTTACCGTAGCGCGCGGCAATCCCTTCTTTTTCACACAGCa > 1:943954/1‑58 (MQ=255) tCATTTGCGGCGGCTTCTTTACCGTAGCGCGCGGCAATCCCTTCTTTTTCACACAGCa > 1:439163/1‑58 (MQ=255) tCATTTGCGGCGGCTTCTTTACCGTAGCGCGCGGCAATCCCTTCTTTTTCACACAGCa > 1:1331713/1‑58 (MQ=255) tCATTTGCGGCGGCTTCTTTACCGTAGCGCGCGGCAATCCCTTCTTTTTCACACAGCa > 1:1578084/1‑58 (MQ=255) tCATTTGCGGCGGCTTCTTTACCGTAGCGCGCGGCAATCCCTTCTTTTTCACACAGCAAGTTAACCGCt > 1:349944/1‑69 (MQ=255) tCATTTGCGGCGGCTTCTTTACCGTAGCGCGCGGCAATCCCTTCTTTTTCACACAGCAAGTTAACCGCt > 1:1175460/1‑69 (MQ=255) tCATTTGCGGCGGCTTCTTTACCGTAGCGCGCGGCAATCCCTTCTTTTTCACACAGCAAGTTAACCGCt > 1:2907656/1‑69 (MQ=255) ttcttTACCGTAGCGCGCGGCAATCCCTTCTTTTTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATa < 1:1244951/69‑1 (MQ=255) ttcttTACCGTAGCGCGCGGCAATCCCTTCTTTTTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATa < 1:2193816/69‑1 (MQ=255) ttcttTACCGTAGCGCGCGGCAATCCCTTCTTTTTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATa < 1:1050773/69‑1 (MQ=255) ttcttTACCGTAGCGCGCGGCAATCCCTTCTTTTTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATa < 1:1826656/69‑1 (MQ=255) ttcttTACCGTAGCGCGCGGCAATCCCTTCTTTTTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATa < 1:761160/69‑1 (MQ=255) ttcttTACCGTAGCGCGCGGCAATCCCTTCTTTTTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATa < 1:2881236/69‑1 (MQ=255) ttcttTACCGTAGCGCGCGGCAATCCCTTCTTTTTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATa < 1:1750474/69‑1 (MQ=255) ttcttTACCGTAGCGCGCGGCAATCCCTTCTTTTTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATa < 1:1134063/69‑1 (MQ=255) ttcttTACCGTAGCGCGCGGCAATCCCTTCTTTTTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATa < 1:3090890/69‑1 (MQ=255) ttcttTACCGTAGCGCGCGGCAATCCCTTCTTTTTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATa < 1:1655020/69‑1 (MQ=255) ttcttTACCGTAGCGCGCGGCAATCCCTTCTTTTTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATa < 1:1347089/69‑1 (MQ=255) ttcttTACCGTAGCGCGCGGCAATCCCTTCTTTTTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATa < 1:3407096/69‑1 (MQ=255) ttcttTACCGTAGCGCGCGGCAATCCCTTCTTTTTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATa < 1:473218/69‑1 (MQ=255) tcttTACCGTAGCGCGCGGCAATCCCTTCTTTTTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATa > 1:985480/1‑68 (MQ=255) tcttTACCGTAGCGCGCGGCAATCCCTTCTTTTTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATAc > 1:3411346/1‑69 (MQ=255) tcttTACCGTAGCGCGCGGCAATCCCTTCTTTTTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATAc > 1:2952226/1‑69 (MQ=255) tcttTACCGTAGCGCGCGGCAATCCCTTCTTTTTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATAc > 1:2975286/1‑69 (MQ=255) tcttTACCGTAGCGCGCGGCAATCCCTTCTTTTTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATAc > 1:680551/1‑69 (MQ=255) tcttTACCGTAGCGCGCGGCAATCCCTTCTTTTTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATAc > 1:1023757/1‑69 (MQ=255) tcttTACCGTAGCGCGCGGCAATCCCTTCTTTTTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATAc > 1:2553523/1‑69 (MQ=255) tcttTACCGTAGCGCGCGGCAATCCCTTCTTTTTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATAc > 1:1701407/1‑69 (MQ=255) tcttTACCGTAGCGCGCGGCAATCCCTTCTTTTTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATAc > 1:2428590/1‑69 (MQ=255) tcttTACCGTAGCGCGCGGCAATCCCTTCTTTTTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATAc > 1:1873888/1‑69 (MQ=255) tcttTACCGTAGCGCGCGGCAATCCCTTCTTTTTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATAc > 1:1229664/1‑69 (MQ=255) tttACCGTAGCGCGCGGCAATCCCTTCTTTCTCACACAGCAAGTTAACCGCtt > 1:2502243/1‑53 (MQ=255) tttACCGTAGCGCGCGGCAATCCCTTCTTTCTCACACAGCAAGTTAACCGCTTc > 1:3191440/1‑54 (MQ=255) tttACCGTAGCGCGCGGCAATCCCTTCTTTCTCACACAGCAAGTTAACCGCTTc > 1:3246491/1‑54 (MQ=255) tttACCGTAGCGCGCGGCAATCCCTTCTTTCTCACACAGCAAGTTAACCGCTTc > 1:3312579/1‑54 (MQ=255) tttACCGTAGCGCGCGGCAATCCCTTCTTTCTCACACAGCAAGTTAACCGCTTc > 1:3088551/1‑54 (MQ=255) tttACCGTAGCGCGCGGCAATCCCTTCTTTCTCACACAGCAAGTTAACCGCTTc > 1:3373674/1‑54 (MQ=255) tttACCGTAGCGCGCGGCAATCCCTTCTTTCTCACACAGCAAGTTAACCGCTTc > 1:351779/1‑54 (MQ=255) tttACCGTAGCGCGCGGCAATCCCTTCTTTCTCACACAGCAAGTTAACCGCTTc > 1:721331/1‑54 (MQ=255) tttACCGTAGCGCGCGGCAATCCCTTCTTTCTCACACAGCAAGTTAACCGCTTc > 1:1107963/1‑54 (MQ=255) tttACCGTAGCGCGCGGCAATCCCTTCTTTCTCACACAGCAAGTTAACCGCTTc > 1:2831965/1‑54 (MQ=255) tttACCGTAGCGCGCGGCAATCCCTTCTTTCTCACACAGCAAGTTAACCGCTTc > 1:1975759/1‑54 (MQ=255) tttACCGTAGCGCGCGGCAATCCCTTCTTTCTCACACAGCAAGTTAACCGCTTc > 1:965371/1‑54 (MQ=255) tttACCGTAGCGCGCGGCAATCCCTTCTTTCTCACACAGCAAGTTAACCGCTTc > 1:2765755/1‑54 (MQ=255) tttACCGTAGCGCGCGGCAATCCCTTCTTTCTCACACAGCAAGTTAACCGCTTc > 1:1970816/1‑54 (MQ=255) tttACCGTAGCGCGCGGCAATCCCTTCTTTCTCACACAGCAAGTTAACCGCTTc > 1:2198091/1‑54 (MQ=255) tttACCGTAGCGCGCGGCAATCCCTTCTTTCTCACACAGCAAGTTAACCGCTTc > 1:1826129/1‑54 (MQ=255) tttACCGTAGCGCGCGGCAATCCCTTCTTTCTCACACAGCAAGTTAACCGCTTc > 1:184968/1‑54 (MQ=255) ttACCGTAGCGCGCGGCAATCCCTTCTTTTTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATACa < 1:2121863/67‑1 (MQ=255) gcgcgcGGCAATCCCTTCTTTTTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATACATGCCAAACAt > 1:745388/1‑69 (MQ=255) gcgcgcGGCAATCCCTTCTTTTTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATACATGCCAAACAt > 1:3371212/1‑69 (MQ=255) gcgcgcGGCAATCCCTTCTTTTTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATACATGCCAAACAt > 1:3079938/1‑69 (MQ=255) cAATCCCTTCTTTTTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATACATGCCAAACATTGGCACaa < 1:1100160/69‑1 (MQ=255) aaTCCCTTCTTTTTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATACATGCCAAACATTGGCACaaa < 1:2328375/69‑1 (MQ=255) aaTCCCTTCTTTTTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATACATGCCAAACATTGGCACaaa < 1:2456396/69‑1 (MQ=255) aaTCCCTTCTTTTTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATACATGCCAAACATTGGCACaaa < 1:664171/69‑1 (MQ=255) aaTCCCTTCTTTTTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATACATGCCAAACATTGGCACaaa < 1:1373248/69‑1 (MQ=255) aaTCCCTTCTTTTTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATACATGCCAAACATTGGCACaaa < 1:1303700/69‑1 (MQ=255) tcttTCTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATAc < 1:484705/42‑1 (MQ=255) tcttTCTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATAc < 1:624075/42‑1 (MQ=255) tcttTCTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATAc < 1:1259340/42‑1 (MQ=255) tcttTCTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATAc < 1:860604/42‑1 (MQ=255) tcttTCTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATAc < 1:1013744/42‑1 (MQ=255) tcttTCTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATAc < 1:2977215/42‑1 (MQ=255) tcttTCTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATAc < 1:2098609/42‑1 (MQ=255) tcttTCTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATAc < 1:2120582/42‑1 (MQ=255) tcttTCTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATAc < 1:1815294/42‑1 (MQ=255) tcttTCTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATAc < 1:2763097/42‑1 (MQ=255) tcttTCTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATAc < 1:2829787/42‑1 (MQ=255) tcttTCTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATAc < 1:1761006/42‑1 (MQ=255) tcttTCTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATAc < 1:2886297/42‑1 (MQ=255) tcttTCTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATAc < 1:1736592/42‑1 (MQ=255) tcttTCTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATAc < 1:348212/42‑1 (MQ=255) tcttTCTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATAc < 1:3018785/42‑1 (MQ=255) tcttTCTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATAc < 1:3056841/42‑1 (MQ=255) tcttTCTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATAc < 1:3206452/42‑1 (MQ=255) tcttTCTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATAc < 1:3316527/42‑1 (MQ=255) tcttTCTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATAc < 1:3367277/42‑1 (MQ=255) tcttTCTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATAc < 1:3404506/42‑1 (MQ=255) tcttTCTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATAc < 1:1307396/42‑1 (MQ=255) | CTGATGCGATAACCTACTTCATTTGCGGCGGCTTCTTTACCGTAGCGCGCGGCAATCCCTTCTTTTTCACACAGCAAGTTAACCGCTTCCGCCAGCCCATACATGCCAAACATTGGCACAAA > minE/2962298‑2962419 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |