| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,962,938 | C→T | 29.6% | V140I (GTT→ATT) | yjjI ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,962,938 | 0 | C | T | 29.6% | 114.1 / 49.9 | 111 | V140I (GTT→ATT) | yjjI | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (28/50); new base T (16/17); total (44/67) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.89e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
Only 100 of 112 total aligned reads displayed.
GCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCA > minE/2962881‑2963000 | gCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGt < 1:1858086/69‑1 (MQ=255)gCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGt < 1:2054924/69‑1 (MQ=255)gCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGt < 1:617621/69‑1 (MQ=255)gCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGt < 1:2634843/69‑1 (MQ=255)gCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGt < 1:2570027/69‑1 (MQ=255)gCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGt < 1:2944346/69‑1 (MQ=255) cATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGt < 1:3077389/68‑1 (MQ=255) gTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTAg < 1:1646507/69‑1 (MQ=255) gTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTAg < 1:3365211/69‑1 (MQ=255) gTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTAg < 1:3215588/69‑1 (MQ=255) gTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTAg < 1:382773/69‑1 (MQ=255) gTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTAg < 1:2075993/69‑1 (MQ=255) gTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTAg < 1:2656723/69‑1 (MQ=255) gTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTAg < 1:2803831/69‑1 (MQ=255) gTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTAg < 1:876942/69‑1 (MQ=255) tCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAATATCGATCTCGTCTTGTGTTAGa < 1:1218571/69‑1 (MQ=255) tCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAATATCGATCTCGTCTTGTGTTAGa < 1:241189/69‑1 (MQ=255) tCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAATATCGATCTCGTCTTGTGTTAGa < 1:1263922/69‑1 (MQ=255) tCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAATATCGATCTCGTCTTGTGTTAGa < 1:710063/69‑1 (MQ=255) tCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAATATCGATCTCGTCTTGTGTTAGa < 1:1106575/69‑1 (MQ=255) tCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAATATCGATCTCGTCTTGTGTTAGa < 1:2660797/69‑1 (MQ=255) tCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAATATCGATCTCGTCTTGTGTTAGa < 1:10826/69‑1 (MQ=255) tCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAATATCGATCTCGTCTTGTGTTAGa < 1:1413689/69‑1 (MQ=255) tCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAATATCGATCTCGTCTTGTGTTAGa < 1:1538879/69‑1 (MQ=255) tCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAATATCGATCTCGTCTTGTGTTAGa < 1:2684492/69‑1 (MQ=255) tCTGGCAGGGTGCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAATATCGATCTCGTCTTGTGTTAGa < 1:1030378/69‑1 (MQ=255) cTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAATATCGATCTCGTCTTGTGTTAGa < 1:2838773/68‑1 (MQ=255) cTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAATATCGATCTCGTCTTGTGTTAGa < 1:3082437/68‑1 (MQ=255) tGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTAg < 1:2012836/66‑1 (MQ=255) gCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAAc > 1:149410/1‑43 (MQ=255) gCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTAGAAtt > 1:3138774/1‑68 (MQ=255) gCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTAGAAtt > 1:3346051/1‑68 (MQ=255) gCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTAGAAtt > 1:1351721/1‑68 (MQ=255) gCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTAGAAtt > 1:129554/1‑68 (MQ=255) gggTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAATATCGATCTCGTCTTGTGTTAGAATTCTaa > 1:2267403/1‑69 (MQ=255) gggTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAATATCGATCTCGTCTTGTGTTAGAATTCTaa > 1:2713673/1‑69 (MQ=255) gggTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAATATCGATCTCGTCTTGTGTTAGAATTCTaa > 1:874004/1‑69 (MQ=255) gggTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAATATCGATCTCGTCTTGTGTTAGAATTCTaa > 1:806458/1‑69 (MQ=255) gggTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAATATCGATCTCGTCTTGTGTTAGAATTCTaa > 1:2937609/1‑69 (MQ=255) gggTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAATATCGATCTCGTCTTGTGTTAGAATTCTaa > 1:2299544/1‑69 (MQ=255) gggTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAATATCGATCTCGTCTTGTGTTAGAATTCTaa > 1:2179747/1‑69 (MQ=255) gggTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAATATCGATCTCGTCTTGTGTTAGAATTCTaa > 1:580365/1‑69 (MQ=255) gggTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAATATCGATCTCGTCTTGTGTTAGAATTCTaa > 1:1596447/1‑69 (MQ=255) gggTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAATATCGATCTCGTCTTGTGTTAGAATTCTaa > 1:3146151/1‑69 (MQ=255) gggTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAATATCGATCTCGTCTTGTGTTAGAATTCTaa > 1:1369427/1‑69 (MQ=255) gggTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAATATCGATCTCGTCTTGTGTTAGAATTCTa > 1:1254495/1‑68 (MQ=255) gggTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAATATCGATCTCGTCTTGTGTTAGAATTCTa > 1:2020539/1‑68 (MQ=255) gTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGtt < 1:2435188/57‑1 (MQ=255) gTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGtt < 1:794644/57‑1 (MQ=255) gTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGtt < 1:267780/57‑1 (MQ=255) gTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACa > 1:1899696/1‑69 (MQ=255) gTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACa > 1:7027/1‑69 (MQ=255) gTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACa > 1:1138534/1‑69 (MQ=255) gTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACa > 1:2937906/1‑69 (MQ=255) gTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACa > 1:106911/1‑69 (MQ=255) gTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACa > 1:2788604/1‑69 (MQ=255) ttCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTAg > 1:2809577/1‑58 (MQ=255) ttCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTAg > 1:3318400/1‑58 (MQ=255) ttCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTAg > 1:948483/1‑58 (MQ=255) ttCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTAg > 1:1702911/1‑58 (MQ=255) ttCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTAg > 1:1065270/1‑58 (MQ=255) ttCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTAg > 1:1068277/1‑58 (MQ=255) ttCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTAg > 1:1323599/1‑58 (MQ=255) ttCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTAg > 1:1629527/1‑58 (MQ=255) ttCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTAg > 1:1800784/1‑58 (MQ=255) atCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTAg > 1:1591355/2‑58 (MQ=255) cTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTAg < 1:3324676/56‑1 (MQ=255) gAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGt < 1:2802325/67‑1 (MQ=255) gAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGt < 1:2351996/67‑1 (MQ=255) gAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGt < 1:1100964/67‑1 (MQ=255) gAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGt < 1:421414/67‑1 (MQ=255) gAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGt < 1:611890/67‑1 (MQ=255) aCGCCAGAAACGTTTTTTTCGAACATCGATCTCGTCTTgtgt < 1:1868292/42‑1 (MQ=255) aCGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTgtgt < 1:2688674/42‑1 (MQ=255) aCGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTgtgt < 1:141799/42‑1 (MQ=255) aCGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTgtgt < 1:611559/42‑1 (MQ=255) aCGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTgtgt < 1:2718463/42‑1 (MQ=255) aCGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTgtgt < 1:746300/42‑1 (MQ=255) aCGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTgtgt < 1:1087056/42‑1 (MQ=255) aCGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTgtgt < 1:2754559/42‑1 (MQ=255) aCGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTgtgt < 1:880967/42‑1 (MQ=255) aCGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTgtgt < 1:90964/42‑1 (MQ=255) aCGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTgtgt < 1:3302388/42‑1 (MQ=255) aCGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTgtgt < 1:2682086/42‑1 (MQ=255) aCGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTgtgt < 1:2398183/42‑1 (MQ=255) aCGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTgtgt < 1:2343295/42‑1 (MQ=255) aCGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTgtgt < 1:1853796/42‑1 (MQ=255) aCGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTgtgt < 1:3121511/42‑1 (MQ=255) aCGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTgtgt < 1:205734/42‑1 (MQ=255) aCGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTgtgt < 1:2199830/42‑1 (MQ=255) aCGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTgtgt < 1:2965608/42‑1 (MQ=255) gCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACg > 1:1110371/1‑69 (MQ=255) gCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACg > 1:42504/1‑69 (MQ=255) aCGTTTTATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAg > 1:2934124/1‑69 (MQ=255) ttATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGc > 1:2109209/1‑68 (MQ=255) ttATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGc > 1:135297/1‑68 (MQ=255) tCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCa < 1:1109187/68‑1 (MQ=255) tCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCa < 1:2702833/68‑1 (MQ=255) tCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCa < 1:1159104/68‑1 (MQ=255) tCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCa < 1:2521343/68‑1 (MQ=255) | GCATAAAGGCGTCTGGCAGGGTTCTGTCGAGGTAACGCCAGAAACGTTTTATTCGAACATCGATCTCGTCTTGTGTTAGAATTCTAACATACGGTTGCAACAACGCATCCAGTTGCCCCA > minE/2962881‑2963000 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |