Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,966,996	A→T	49.2%	V368V (GTA→GTT)	deoB →	phosphopentomutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,966,996	0	A	T	49.2%	‑2.7 / 81.9	67	V368V (GTA→GTT)	deoB	phosphopentomutase
Reads supporting (aligned to +/- strand):  ref base A (8/26);  new base T (30/3);  total (38/29)
Fisher's exact test for biased strand distribution p-value = 1.62e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGACCACGGTTGCGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCT  >  minE/2966929‑2967064                                                                    |                                                                     cTGACCACGGTTGCGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGta                                                                      <  1:227854/68‑1 (MQ=255)                aTCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTaa                                                      <  1:2166014/69‑1 (MQ=255)                aTCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTaa                                                      <  1:2729085/69‑1 (MQ=255)                aTCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTaa                                                      <  1:2383792/69‑1 (MQ=255)                aTCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTaa                                                      <  1:219609/69‑1 (MQ=255)                aTCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTaa                                                      <  1:636872/69‑1 (MQ=255)                aTCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTaa                                                      <  1:832003/69‑1 (MQ=255)                 tCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTaaa                                                     <  1:1483373/69‑1 (MQ=255)                            ccGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTc                                            <  1:1147740/67‑1 (MQ=255)                            ccGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTc                                            <  1:1257427/67‑1 (MQ=255)                            ccGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTc                                            <  1:3422900/67‑1 (MQ=255)                            ccGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTc                                            <  1:1514901/67‑1 (MQ=255)                                    gACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTAGGTCa                                   <  1:344902/68‑1 (MQ=255)                                      ccACACGCGTGAACACATTCCGGTACTGGTTTACTGCCCGAAAGTAAAACCGGGTTc                                            >  1:2869903/1‑57 (MQ=255)                                      ccACACGCGTGAACACATTCCGGTACTGGTTTACGGccc                                                              >  1:2435331/1‑39 (MQ=38)                                      ccACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGaa                                                           >  1:1160335/1‑42 (MQ=38)                                      ccACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTaaaa                                                    >  1:1739222/1‑49 (MQ=39)                                      ccACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTaa                                                      >  1:1507890/1‑47 (MQ=39)                                      ccACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCg                                                 >  1:1862450/1‑52 (MQ=255)                                      ccACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTc                                            >  1:251708/1‑57 (MQ=255)                                      ccACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTc                                            >  1:917592/1‑57 (MQ=255)                                      ccACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTc                                            >  1:2427772/1‑57 (MQ=255)                                      ccACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTc                                            >  1:2876033/1‑57 (MQ=255)                                      ccACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTc                                            >  1:3037688/1‑57 (MQ=255)                                      ccACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTc                                            >  1:2375157/1‑57 (MQ=255)                                      ccACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTc                                            >  1:3376454/1‑57 (MQ=255)                                      ccACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTc                                            >  1:517336/1‑57 (MQ=255)                                      ccACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTc                                            >  1:2195987/1‑57 (MQ=255)                                      ccACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTc                                            >  1:1028085/1‑57 (MQ=255)                                      ccACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTc                                            >  1:2072470/1‑57 (MQ=255)                                      ccACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTc                                            >  1:1076946/1‑57 (MQ=255)                                      ccACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTc                                            >  1:1166362/1‑57 (MQ=255)                                      ccACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTc                                            >  1:1283643/1‑57 (MQ=255)                                      ccACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTc                                            >  1:2099928/1‑57 (MQ=255)                                      ccACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTc                                            >  1:1393940/1‑57 (MQ=255)                                      ccACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTc                                            >  1:1538653/1‑57 (MQ=255)                                      ccACACGCGTGAACACATTACGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTc                                            >  1:1649146/1‑57 (MQ=255)                                                 aaCACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTCACTGGGTCACCGTGAAACCt                        >  1:2066936/1‑66 (MQ=255)                                                 aaCACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTCACTGGGTCACCGTGAAACCTTcg                     >  1:3019642/1‑69 (MQ=255)                                                 aaCACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTCACTGGGTCACCGTGAAACCTTcg                     >  1:784208/1‑69 (MQ=255)                                                 aaCACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTCACTGGGTCACCGTGAAACCTTcg                     >  1:2603156/1‑69 (MQ=255)                                                 aaCACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTCACTGGGTCACCGTGAAACCTTcg                     >  1:2474907/1‑69 (MQ=255)                                                 aaCACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTCACTGGGTCACCGTGAAACCTTcg                     >  1:2322055/1‑69 (MQ=255)                                                 aaCACATTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTcg                     >  1:572533/1‑69 (MQ=255)                                                   cacaTTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTcgcg                   <  1:1041005/69‑1 (MQ=255)                                                   cacaTTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTcgcg                   <  1:813718/69‑1 (MQ=255)                                                   cacaTTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTcgcg                   <  1:374158/69‑1 (MQ=255)                                                   cacaTTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTcgcg                   <  1:1245546/69‑1 (MQ=255)                                                   cacaTTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTcgcg                   <  1:2064200/69‑1 (MQ=255)                                                   cacaTTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTcgcg                   <  1:1307839/69‑1 (MQ=255)                                                   cacaTTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTcgcg                   <  1:3282119/69‑1 (MQ=255)                                                   cacaTTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTcgcg                   <  1:294595/69‑1 (MQ=255)                                                      aTTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTCACt                                         <  1:1550321/44‑1 (MQ=37)                                                      aTTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTCACt                                         <  1:879639/44‑1 (MQ=37)                                                      aTTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTCACt                                         <  1:1131541/44‑1 (MQ=37)                                                      aTTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGat                <  1:2792554/69‑1 (MQ=255)                                                      aTTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGat                <  1:222107/69‑1 (MQ=255)                                                      aTTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGat                <  1:742809/69‑1 (MQ=255)                                                      aTTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGat                <  1:1334358/69‑1 (MQ=255)                                                       ttCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGat                <  1:2734552/68‑1 (MQ=255)                                                       ttCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGat                <  1:1933819/68‑1 (MQ=255)                                                         ccGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATc             >  1:385968/1‑69 (MQ=255)                                                         ccGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATc             >  1:1630622/1‑69 (MQ=255)                                                         ccGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATc             >  1:3007452/1‑69 (MQ=255)                                                         ccGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATc             >  1:755878/1‑69 (MQ=255)                                                         ccGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATc             >  1:1544630/1‑69 (MQ=255)                                                                  gTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGAct    >  1:3261606/1‑69 (MQ=255)                                                                  gTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGAct    >  1:3234915/1‑69 (MQ=255)                                                                    atatGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGActct  >  1:2444180/1‑69 (MQ=255)                                                                    atatGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGActct  >  1:660042/1‑69 (MQ=255)                                                                    atatGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGActct  >  1:2198776/1‑69 (MQ=255)                                                                    atatGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGActct  >  1:3176659/1‑69 (MQ=255)                                                                    |                                                                     CTGACCACGGTTGCGATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCT  >  minE/2966929‑2967064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑