Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,966,999	T→C	49.3%	Y369Y (TAT→TAC)	deoB →	phosphopentomutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,966,999	0	T	C	49.3%	‑6.4 / 61.7	67	Y369Y (TAT→TAC)	deoB	phosphopentomutase
Reads supporting (aligned to +/- strand):  ref base T (8/26);  new base C (30/3);  total (38/29)
Fisher's exact test for biased strand distribution p-value = 1.62e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTGG  >  minE/2966944‑2967066                                                        |                                                                    aTCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTaa                                                        <  1:832003/69‑1 (MQ=255) aTCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTaa                                                        <  1:636872/69‑1 (MQ=255) aTCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTaa                                                        <  1:2166014/69‑1 (MQ=255) aTCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTaa                                                        <  1:219609/69‑1 (MQ=255) aTCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTaa                                                        <  1:2383792/69‑1 (MQ=255) aTCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTaa                                                        <  1:2729085/69‑1 (MQ=255)  tCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTaaa                                                       <  1:1483373/69‑1 (MQ=255)             ccGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTc                                              <  1:1147740/67‑1 (MQ=255)             ccGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTc                                              <  1:3422900/67‑1 (MQ=255)             ccGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTc                                              <  1:1257427/67‑1 (MQ=255)             ccGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTc                                              <  1:1514901/67‑1 (MQ=255)                     gACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTAGGTCa                                     <  1:344902/68‑1 (MQ=255)                       ccACACGCGTGAACACATTCCGGTACTGGTTTACTGCCCGAAAGTAAAACCGGGTTc                                              >  1:2869903/1‑57 (MQ=255)                       ccACACGCGTGAACACATTCCGGTACTGGTTTACGGccc                                                                >  1:2435331/1‑39 (MQ=38)                       ccACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGaa                                                             >  1:1160335/1‑42 (MQ=38)                       ccACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTaaaa                                                      >  1:1739222/1‑49 (MQ=39)                       ccACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTaa                                                        >  1:1507890/1‑47 (MQ=39)                       ccACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCg                                                   >  1:1862450/1‑52 (MQ=255)                       ccACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTc                                              >  1:251708/1‑57 (MQ=255)                       ccACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTc                                              >  1:2876033/1‑57 (MQ=255)                       ccACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTc                                              >  1:3037688/1‑57 (MQ=255)                       ccACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTc                                              >  1:2375157/1‑57 (MQ=255)                       ccACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTc                                              >  1:3376454/1‑57 (MQ=255)                       ccACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTc                                              >  1:2195987/1‑57 (MQ=255)                       ccACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTc                                              >  1:517336/1‑57 (MQ=255)                       ccACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTc                                              >  1:2099928/1‑57 (MQ=255)                       ccACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTc                                              >  1:2072470/1‑57 (MQ=255)                       ccACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTc                                              >  1:917592/1‑57 (MQ=255)                       ccACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTc                                              >  1:2427772/1‑57 (MQ=255)                       ccACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTc                                              >  1:1028085/1‑57 (MQ=255)                       ccACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTc                                              >  1:1393940/1‑57 (MQ=255)                       ccACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTc                                              >  1:1283643/1‑57 (MQ=255)                       ccACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTc                                              >  1:1166362/1‑57 (MQ=255)                       ccACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTc                                              >  1:1538653/1‑57 (MQ=255)                       ccACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTc                                              >  1:1076946/1‑57 (MQ=255)                       ccACACGCGTGAACACATTACGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTc                                              >  1:1649146/1‑57 (MQ=255)                                  aaCACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTCACTGGGTCACCGTGAAACCt                          >  1:2066936/1‑66 (MQ=255)                                  aaCACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTCACTGGGTCACCGTGAAACCTTcg                       >  1:784208/1‑69 (MQ=255)                                  aaCACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTCACTGGGTCACCGTGAAACCTTcg                       >  1:3019642/1‑69 (MQ=255)                                  aaCACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTCACTGGGTCACCGTGAAACCTTcg                       >  1:2322055/1‑69 (MQ=255)                                  aaCACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTCACTGGGTCACCGTGAAACCTTcg                       >  1:2603156/1‑69 (MQ=255)                                  aaCACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTCACTGGGTCACCGTGAAACCTTcg                       >  1:2474907/1‑69 (MQ=255)                                  aaCACATTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTcg                       >  1:572533/1‑69 (MQ=255)                                    cacaTTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTcgcg                     <  1:813718/69‑1 (MQ=255)                                    cacaTTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTcgcg                     <  1:294595/69‑1 (MQ=255)                                    cacaTTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTcgcg                     <  1:1307839/69‑1 (MQ=255)                                    cacaTTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTcgcg                     <  1:3282119/69‑1 (MQ=255)                                    cacaTTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTcgcg                     <  1:1245546/69‑1 (MQ=255)                                    cacaTTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTcgcg                     <  1:2064200/69‑1 (MQ=255)                                    cacaTTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTcgcg                     <  1:374158/69‑1 (MQ=255)                                    cacaTTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTcgcg                     <  1:1041005/69‑1 (MQ=255)                                       aTTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTCACt                                           <  1:1131541/44‑1 (MQ=37)                                       aTTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTCACt                                           <  1:879639/44‑1 (MQ=37)                                       aTTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTCACt                                           <  1:1550321/44‑1 (MQ=37)                                       aTTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGat                  <  1:222107/69‑1 (MQ=255)                                       aTTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGat                  <  1:2792554/69‑1 (MQ=255)                                       aTTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGat                  <  1:742809/69‑1 (MQ=255)                                       aTTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGat                  <  1:1334358/69‑1 (MQ=255)                                        ttCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGat                  <  1:1933819/68‑1 (MQ=255)                                        ttCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGat                  <  1:2734552/68‑1 (MQ=255)                                          ccGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATc               >  1:1630622/1‑69 (MQ=255)                                          ccGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATc               >  1:3007452/1‑69 (MQ=255)                                          ccGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATc               >  1:755878/1‑69 (MQ=255)                                          ccGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATc               >  1:1544630/1‑69 (MQ=255)                                          ccGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATc               >  1:385968/1‑69 (MQ=255)                                                   gTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGAct      >  1:3234915/1‑69 (MQ=255)                                                   gTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGAct      >  1:3261606/1‑69 (MQ=255)                                                     atatGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGActct    >  1:2198776/1‑69 (MQ=255)                                                     atatGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGActct    >  1:3176659/1‑69 (MQ=255)                                                     atatGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGActct    >  1:660042/1‑69 (MQ=255)                                                     atatGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGActct    >  1:2444180/1‑69 (MQ=255)                                                       atGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTg   >  1:789446/1‑68 (MQ=255)                                                       atGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTg   >  1:1583355/1‑68 (MQ=255)                                                       atGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTg   >  1:1477851/1‑68 (MQ=255)                                                        tGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCg                                  >  1:2856872/1‑36 (MQ=255)                                                        tGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGAc       <  1:1011463/63‑1 (MQ=255)                                                        tGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGAc       <  1:1524735/63‑1 (MQ=255)                                                        tGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGAc       <  1:603852/63‑1 (MQ=255)                                                        tGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGAc       <  1:2349171/63‑1 (MQ=255)                                                        tGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGAc       <  1:1451166/63‑1 (MQ=255)                                                        tGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGAc       <  1:676824/63‑1 (MQ=255)                                                        tGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGAc       <  1:313876/63‑1 (MQ=255)                                                        tGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGAc       <  1:3133194/63‑1 (MQ=255)                                                        tGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGAc       <  1:2769223/63‑1 (MQ=255)                                                        tGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGAc       <  1:2905346/63‑1 (MQ=255)                                                        tGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGAc       <  1:1935322/63‑1 (MQ=255)                                                        tGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGAc       <  1:1912817/63‑1 (MQ=255)                                                        tGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTgg  >  1:629928/1‑68 (MQ=255)                                                        tGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTgg  >  1:2821221/1‑68 (MQ=255)                                                        tGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTgg  >  1:1649992/1‑68 (MQ=255)                                                        |                                                                    ATCCGACCTGGACCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTGG  >  minE/2966944‑2967066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑