Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,967,020	C→T	30.0%	G376G (GGC→GGT)	deoB →	phosphopentomutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,967,020	0	C	T	30.0%	91.3 / 42.2	93	G376G (GGC→GGT)	deoB	phosphopentomutase
Reads supporting (aligned to +/- strand):  ref base C (22/43);  new base T (25/3);  total (47/46)
Fisher's exact test for biased strand distribution p-value = 7.77e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

CCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTGGCAAAATATTTTGGTACTTCTGA  >  minE/2966956‑2967088                                                                 |                                                                     ccGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTc                                                                    <  1:1514901/67‑1 (MQ=255) ccGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTc                                                                    <  1:3422900/67‑1 (MQ=255) ccGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTc                                                                    <  1:1147740/67‑1 (MQ=255) ccGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTc                                                                    <  1:1257427/67‑1 (MQ=255)         gACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTAGGTCa                                                           <  1:344902/68‑1 (MQ=255)           ccACACGCGTGAACACATTCCGGTACTGGTTTACTGCCCGAAAGTAAAACCGGGTTc                                                                    >  1:2869903/1‑57 (MQ=255)           ccACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTc                                                                    >  1:2876033/1‑57 (MQ=255)           ccACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTc                                                                    >  1:1538653/1‑57 (MQ=255)           ccACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTc                                                                    >  1:2072470/1‑57 (MQ=255)           ccACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTc                                                                    >  1:2099928/1‑57 (MQ=255)           ccACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTc                                                                    >  1:2195987/1‑57 (MQ=255)           ccACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTc                                                                    >  1:2375157/1‑57 (MQ=255)           ccACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTc                                                                    >  1:2427772/1‑57 (MQ=255)           ccACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTc                                                                    >  1:251708/1‑57 (MQ=255)           ccACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTc                                                                    >  1:1028085/1‑57 (MQ=255)           ccACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTc                                                                    >  1:3037688/1‑57 (MQ=255)           ccACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTc                                                                    >  1:3376454/1‑57 (MQ=255)           ccACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTc                                                                    >  1:517336/1‑57 (MQ=255)           ccACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTc                                                                    >  1:917592/1‑57 (MQ=255)           ccACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTc                                                                    >  1:1283643/1‑57 (MQ=255)           ccACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTc                                                                    >  1:1076946/1‑57 (MQ=255)           ccACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTc                                                                    >  1:1166362/1‑57 (MQ=255)           ccACACGCGTGAACACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTc                                                                    >  1:1393940/1‑57 (MQ=255)           ccACACGCGTGAACACATTACGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTc                                                                    >  1:1649146/1‑57 (MQ=255)                      aaCACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTCACTGGGTCACCGTGAAACCt                                                >  1:2066936/1‑66 (MQ=255)                      aaCACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTCACTGGGTCACCGTGAAACCTTcg                                             >  1:2322055/1‑69 (MQ=255)                      aaCACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTCACTGGGTCACCGTGAAACCTTcg                                             >  1:2474907/1‑69 (MQ=255)                      aaCACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTCACTGGGTCACCGTGAAACCTTcg                                             >  1:3019642/1‑69 (MQ=255)                      aaCACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTCACTGGGTCACCGTGAAACCTTcg                                             >  1:784208/1‑69 (MQ=255)                      aaCACATTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTCACTGGGTCACCGTGAAACCTTcg                                             >  1:2603156/1‑69 (MQ=255)                      aaCACATTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTcg                                             >  1:572533/1‑69 (MQ=255)                        cacaTTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTcgcg                                           <  1:294595/69‑1 (MQ=255)                        cacaTTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTcgcg                                           <  1:813718/69‑1 (MQ=255)                        cacaTTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTcgcg                                           <  1:1307839/69‑1 (MQ=255)                        cacaTTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTcgcg                                           <  1:374158/69‑1 (MQ=255)                        cacaTTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTcgcg                                           <  1:2064200/69‑1 (MQ=255)                        cacaTTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTcgcg                                           <  1:1041005/69‑1 (MQ=255)                        cacaTTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTcgcg                                           <  1:1245546/69‑1 (MQ=255)                        cacaTTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTcgcg                                           <  1:3282119/69‑1 (MQ=255)                           aTTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTCACt                                                                 <  1:879639/44‑1 (MQ=37)                           aTTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTCACt                                                                 <  1:1550321/44‑1 (MQ=37)                           aTTCCGGTACTGGTTTACGGCCCGAAAGTAAAACCGGGTTCACt                                                                 <  1:1131541/44‑1 (MQ=37)                           aTTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGat                                        <  1:2792554/69‑1 (MQ=255)                           aTTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGat                                        <  1:742809/69‑1 (MQ=255)                           aTTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGat                                        <  1:1334358/69‑1 (MQ=255)                           aTTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGat                                        <  1:222107/69‑1 (MQ=255)                            ttCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGat                                        <  1:2734552/68‑1 (MQ=255)                            ttCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGat                                        <  1:1933819/68‑1 (MQ=255)                              ccGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATc                                     >  1:3007452/1‑69 (MQ=255)                              ccGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATc                                     >  1:385968/1‑69 (MQ=255)                              ccGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATc                                     >  1:755878/1‑69 (MQ=255)                              ccGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATc                                     >  1:1630622/1‑69 (MQ=255)                              ccGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATc                                     >  1:1544630/1‑69 (MQ=255)                                       gTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGAct                            >  1:3261606/1‑69 (MQ=255)                                       gTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGAct                            >  1:3234915/1‑69 (MQ=255)                                         atatGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGActct                          >  1:3176659/1‑69 (MQ=255)                                         atatGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGActct                          >  1:2444180/1‑69 (MQ=255)                                         atatGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGActct                          >  1:2198776/1‑69 (MQ=255)                                         atatGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGActct                          >  1:660042/1‑69 (MQ=255)                                           atGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTg                         >  1:789446/1‑68 (MQ=255)                                           atGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTg                         >  1:1477851/1‑68 (MQ=255)                                           atGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTg                         >  1:1583355/1‑68 (MQ=255)                                            tGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCg                                                        >  1:2856872/1‑36 (MQ=255)                                            tGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGAc                             <  1:676824/63‑1 (MQ=255)                                            tGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGAc                             <  1:603852/63‑1 (MQ=255)                                            tGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGAc                             <  1:1011463/63‑1 (MQ=255)                                            tGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGAc                             <  1:3133194/63‑1 (MQ=255)                                            tGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGAc                             <  1:2905346/63‑1 (MQ=255)                                            tGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGAc                             <  1:1524735/63‑1 (MQ=255)                                            tGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGAc                             <  1:313876/63‑1 (MQ=255)                                            tGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGAc                             <  1:1912817/63‑1 (MQ=255)                                            tGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGAc                             <  1:2769223/63‑1 (MQ=255)                                            tGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGAc                             <  1:1935322/63‑1 (MQ=255)                                            tGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGAc                             <  1:2349171/63‑1 (MQ=255)                                            tGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGAc                             <  1:1451166/63‑1 (MQ=255)                                            tGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTgg                        >  1:629928/1‑68 (MQ=255)                                            tGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTgg                        >  1:1649992/1‑68 (MQ=255)                                            tGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTgg                        >  1:2821221/1‑68 (MQ=255)                                                ccGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTGGCaaaa                   <  1:2054884/69‑1 (MQ=255)                                                ccGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTGGCaaaa                   <  1:3372396/69‑1 (MQ=255)                                                ccGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTGGCaaaa                   <  1:3368937/69‑1 (MQ=255)                                                 cGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTGGCaaaa                   <  1:1379048/68‑1 (MQ=255)                                                     aGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTGGCAAAATAttt              >  1:1262938/1‑69 (MQ=255)                                                     aGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTGGCAAAATAttt              >  1:429767/1‑69 (MQ=255)                                                     aGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTGGCAAAATAttt              >  1:3323486/1‑69 (MQ=255)                                                        aaaaCCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTGGCAAAATATTTTgg           <  1:62836/69‑1 (MQ=255)                                                        aaaaCCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTGGCAAAATATTTTgg           <  1:2954647/69‑1 (MQ=255)                                                        aaaaCCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTGGCAAAATATTTTgg           <  1:2708284/69‑1 (MQ=255)                                                        aaaaCCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTGGCAAAATATTTTgg           <  1:2593740/69‑1 (MQ=255)                                                        aaaaCCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTGGCAAAATATTTTgg           <  1:2206464/69‑1 (MQ=255)                                                        aaaaCCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTGGCAAAATATTTTgg           <  1:151951/69‑1 (MQ=255)                                                          aaCCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGat                                        <  1:81491/38‑1 (MQ=255)                                                             cGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGAc                             <  1:2092664/46‑1 (MQ=255)                                                                 cTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTGGCAAAATATTTTGGTACTTCTGa  <  1:697200/69‑1 (MQ=255)                                                                 cTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTGGCAAAATATTTTGGTACTTCTGa  <  1:2752668/69‑1 (MQ=255)                                                                 |                                                                     CCGGTACTGACCACACGCGTGAACACATTCCGGTACTGGTATATGGCCCGAAAGTAAAACCGGGCTCACTGGGTCATCGTGAAACCTTCGCGGATATCGGCCAGACTCTGGCAAAATATTTTGGTACTTCTGA  >  minE/2966956‑2967088

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑