| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,971,610 | A→G | 38.6% | E142E (GAA→GAG) | serB → | 3‑phosphoserine phosphatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,971,610 | 0 | A | G | 38.6% | 17.5 / 26.9 | 44 | E142E (GAA→GAG) | serB | 3‑phosphoserine phosphatase |
| Reads supporting (aligned to +/- strand): ref base A (7/20); new base G (11/6); total (18/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.45e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01 | |||||||||||
AGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAAC‑GGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGTGTGGCGACG > minE/2971552‑2971676 | aGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAGGTAACCGAAc > 1:1309882/1‑69 (MQ=255)aGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAGGTAACCGAAc > 1:923903/1‑69 (MQ=255)aGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAGGTAACCGAAc > 1:557603/1‑69 (MQ=255)aGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAGGTAACCGAAc > 1:1402357/1‑69 (MQ=255)aGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAGGTAACCGAAc > 1:1406093/1‑69 (MQ=255)aGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAGGTAACCGAAc > 1:1413233/1‑69 (MQ=255)aGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAGGTAACCGAAc > 1:319889/1‑69 (MQ=255)aGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAGGTAACCGAAc > 1:2534017/1‑69 (MQ=255)aGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAGGTAACCGAAc > 1:2333798/1‑69 (MQ=255)aGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAGGTAACCGAAc > 1:1991907/1‑69 (MQ=255)aGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAGGTAACCGAAc > 1:1574719/1‑69 (MQ=255) tGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAAC‑GGGCGAt > 1:1348819/1‑68 (MQ=255) tGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAAC‑GGGCGATg > 1:85573/1‑69 (MQ=255) tGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAAC‑GGGCGATg > 1:1385206/1‑69 (MQ=255) tGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAAC‑GGGCGATg > 1:2550391/1‑69 (MQ=255) aTTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGt > 1:971247/1‑50 (MQ=255) aTTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGt < 1:3161993/50‑1 (MQ=255) aTTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGt < 1:3154265/50‑1 (MQ=255) aTTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGt > 1:3080361/1‑50 (MQ=255) aTTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGt < 1:1502814/50‑1 (MQ=255) aTTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGt > 1:2905747/1‑50 (MQ=255) aTTGATGAAATTGCAAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGt < 1:2509530/50‑1 (MQ=255) gatgaAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAAC‑g < 1:1977921/56‑1 (MQ=255) gatgaAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAAC‑g < 1:1383247/56‑1 (MQ=255) gatgaAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAAC‑g < 1:3043656/56‑1 (MQ=255) gatgaAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAAC‑g < 1:254769/56‑1 (MQ=255) gatgaAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAAC‑g < 1:23569/56‑1 (MQ=255) gatgaAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAAC‑g < 1:1533911/56‑1 (MQ=255) atgaAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAAC‑g < 1:2353793/55‑1 (MQ=255) ttGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAGGTAACCGAAC‑GGGCGATGCGCGGCGAACTCg < 1:1756949/69‑1 (MQ=255) ttGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAGGTAACCGAAC‑GGGCGATGCGCGGCGAACTCg < 1:1553995/69‑1 (MQ=255) ttGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAGGTAACCGAAC‑GGGCGATGCGCGGCGAACTCg < 1:2062490/69‑1 (MQ=255) ttGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAGGTAACCGAAC‑GGGCGATGCGCGGCGAACTCg < 1:3345406/69‑1 (MQ=255) ttGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAGGTAACCGAAC‑GGGCGATGCGCGGCGAACTCg < 1:1428777/69‑1 (MQ=255) ttGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAAC‑GGGCGATGCGCGGCGAACTCg < 1:772586/69‑1 (MQ=255) ttGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAAC‑GGGCGATGCGCGGCGAACTCg < 1:908634/69‑1 (MQ=255) aaaCTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAAC‑GGGCGATGCGCGGCGAACTCGAtttt < 1:3060695/69‑1 (MQ=255) aaaCTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAAC‑GGGCGATGCGCGGCGAACTCGAtttt < 1:595819/69‑1 (MQ=255) aaCTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAAC‑GGGCGATGCGCGGCGAACTCGAtttt < 1:2273172/68‑1 (MQ=255) tGGCCGGAAGGGGCGAGATGGTGGCGGAAGTAACCGAAC‑g < 1:3260603/40‑1 (MQ=255) cGAGATGGTGGCGGAAGTAACCGAACGGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCa < 1:32140/69‑1 (MQ=255) cGAGATGGTGGCGGAAGTAACCGAACGGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCa < 1:2558982/69‑1 (MQ=255) cGAGATGGTGGCGGAAGTAACCGAACGGGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCATGCGCa < 1:2382001/69‑1 (MQ=39) aTGGTGGCGGAGGTAACCGAAC‑GGGCGATGCGCGGCGAACTCg < 1:359770/43‑1 (MQ=255) aGTAACCGAAC‑GGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGTGTGGCGACg > 1:3047221/1‑67 (MQ=255) | AGATTGAATGTATTGATGAAATTGCCAAACTGGCCGGAACGGGCGAGATGGTGGCGGAAGTAACCGAAC‑GGGCGATGCGCGGCGAACTCGATTTTACCGCCAGCCTGCGCAGCCGTGTGGCGACG > minE/2971552‑2971676 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |