| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,973,590 | T→G | 54.4% | intergenic (+6/‑15) | radA → / → nadR | predicted repair protein/bifunctional DNA‑binding transcriptional repressor and NMN adenylyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,973,590 | 0 | T | G | 54.4% | 14.3 / 43.4 | 46 | intergenic (+6/‑15) | radA/nadR | predicted repair protein/bifunctional DNA‑binding transcriptional repressor and NMN adenylyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (5/16); new base G (21/4); total (26/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.71e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01 | |||||||||||
TGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGC > minE/2973536‑2973652 | tGGCGTTAAAAAACTCTCCGACGCGCTTATCGTGTTCGACGACTTATAATGAGATATACGGAGGgaga < 1:1020587/68‑1 (MQ=255)tGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGATATACGGAGGgaga < 1:2453238/68‑1 (MQ=255)tGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGATATACGGAGGgaga < 1:3183153/68‑1 (MQ=255)tGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGATATACGGAGGgaga < 1:2208495/68‑1 (MQ=255)tGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGATATACGGAGGgaga < 1:563167/68‑1 (MQ=255)tGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGATATACGGAGGgaga < 1:1859884/68‑1 (MQ=255) aaaaaaCTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGATATACg < 1:2717479/53‑1 (MQ=255) aaaaaaCTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGATATACg < 1:2927027/53‑1 (MQ=255) aaaaaaCTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGATATACGGAGGGAGATATgtcgt < 1:2936668/69‑1 (MQ=255) ctctCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGATATACGGAGGGAGATATGTCGTCATTTg < 1:102522/69‑1 (MQ=255) ctctCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGATATACGGAGGGAGATATGTCGTCATTTg < 1:431481/69‑1 (MQ=255) cgcgCTTAGCGTGTTCGACGACTTATAATGAGAGATACGGAGGTAGATATGTCGTCATTTGATTACCt > 1:2793820/1‑68 (MQ=255) cgcgCTTAGCGTGTTCGACGACTTATAATGAGAGATACGGAGGGAGATATGTCGTCATTTGAtt > 1:1933413/1‑64 (MQ=255) cgcgCTTAGCGTGTTCGACGACTTATAATGAGAGATACGGAGGGAGATATGTCGTCATTTGAtt > 1:1323105/1‑64 (MQ=255) cgcgCTTAGCGTGTTCGACGACTTATAATGAGAGATACGGAGGGAGATATGTCGTCATTTGATTACCt > 1:490610/1‑68 (MQ=255) cgcgCTTAGCGTGTTCGACGACTTATAATGAGAGATACGGAGGGAGATATGTCGTCATTTGATTACCt > 1:270583/1‑68 (MQ=255) cgcgCTTAGCGTGTTCGACGACTTATAATGAGAGATACGGAGGGAGATATGTCGTCATTTGATTACCt > 1:565602/1‑68 (MQ=255) cgcgCTTAGCGTGTTCGACGACTTATAATGAGAGATACGGAGGGAGATATGTCGTCATTTGATTACCt > 1:2504698/1‑68 (MQ=255) cgcgCTTAGCGTGTTCGACGACTTATAATGAGAGATACGGAGGGAGATATGTCGTCATTTGATTACCt > 1:1123330/1‑68 (MQ=255) cgcgCTTAGCGTGTTCGACGACTTATAATGAGAGATACGGAGGGAGATATGTCGTCATTTGATTACCt > 1:1209676/1‑68 (MQ=255) cgcgCTTAGCGTGTTCGACGACTTATAATGAGAGATACGGAGGGAGATATGTCGTCATTTGATTACCt > 1:2450061/1‑68 (MQ=255) cgcgCTTAGCGTGTTCGACGACTTATAATGAGAGATACGGAGGGAGATATGTCGTCATTTGATTACCt > 1:2334491/1‑68 (MQ=255) cgcgCTTAGCGTGTTCGACGACTTATAATGAGAGATACGGAGGGAGATATGTCGTCATTTGATTACCt > 1:2313116/1‑68 (MQ=255) cgcgCTTAGCGTGTTCGACGACTTATAATGAGAGATACGGAGGGAGATATGTCGTCATTTGATTACCt > 1:1238523/1‑68 (MQ=255) cgcgCTTAGCGTGTTCGACGACTTATAATGAGAGATACGGAGGGAGATATGTCGTCATTTGATTACCt > 1:3194645/1‑68 (MQ=255) cgcgCTTAGCGTGTTCGACGACTTATAATGAGAGATACGGAGGGAGATATGTCGTCATTTGATTACCt > 1:2074686/1‑68 (MQ=255) cgcgCTTAGCGTGTTCGACGACTTATAATGAGAGATACGGAGGGAGATATGTCGTCATTTGATTACCt > 1:1565204/1‑68 (MQ=255) cgcgCTTAGCGTGTTCGACGACTTATAATGAGAGATACGGAGGGAGATATGTCGTCATTTGATTACCt > 1:1908769/1‑68 (MQ=255) cgcgCTTAGCGTGTTCGACGACTTATAATGAGAGATACGGAGGGAGATATGTCGTCATTTGATTACCt > 1:186220/1‑68 (MQ=255) cgcgCTTAGCGTGTTCGACGACTTATAATGAGAGATACGGAGGGAGATATGTCGTCATTTGATTACCt > 1:1619861/1‑68 (MQ=255) cgcgCTTAGCGTGTTCGACGACTTATAATGAGAGATACGGAGGGAGATATGTCGTCATTTGATTACCt > 1:535226/1‑68 (MQ=255) cgcgCTGAGCGTGTTCGACGACTTATAATGAGAGATACGGAGGGAGATATGTCGTCATTTGATTACCt > 1:437638/1‑68 (MQ=255) cgcTTAGCGTGTTCGACGACTTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGaa < 1:568197/69‑1 (MQ=255) cgcTTAGCGTGTTCGACGACTTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGaa < 1:2290776/69‑1 (MQ=255) cgcTTAGCGTGTTCGACGACTTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGaa < 1:2601494/69‑1 (MQ=255) cgcTTAGCGTGTTCGACGACTTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGaa < 1:1086602/69‑1 (MQ=255) gcTTAGCGTGTTCGACGACTTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGaa < 1:2164921/68‑1 (MQ=255) ttCGACGACTTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTAcc > 1:1377022/1‑54 (MQ=255) ttCGACGACTTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCa > 1:2458703/1‑69 (MQ=255) ttCGACGACTTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCa > 1:1062437/1‑69 (MQ=255) cTTATAATGAGAGATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAg < 1:1002808/69‑1 (MQ=255) cTTATAATGAGAGATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAg < 1:1689937/69‑1 (MQ=255) cTTATAATGAGAGATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAg < 1:610860/69‑1 (MQ=255) taATGAGAGATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAg < 1:1967294/65‑1 (MQ=255) aTGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGc > 1:1929327/1‑69 (MQ=255) aTGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGc > 1:1767860/1‑69 (MQ=255) | TGGCGTTAAAAAACTCTCCGACGCGCTTAGCGTGTTCGACGACTTATAATGAGATATACGGAGGGAGATATGTCGTCATTTGATTACCTGAAAACTGCCATCAAGCAACAGGGCTGC > minE/2973536‑2973652 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |