| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 253,031 | A→G | 79.8% | R119R (CGT→CGC) | yajB ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 253,031 | 0 | A | G | 79.8% | 62.7 / 9.4 | 49 | R119R (CGT→CGC) | yajB | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (2/8); new base G (24/15); total (26/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.15e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCG > minE/252965‑253098 | gAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATaaaatg > 1:2472950/1‑67 (MQ=255)gAAATCCATATCGCGATAGCGCACAAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACg > 1:1799779/1‑69 (MQ=255) accaaccaCTGCTCTGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCGGTGAGTCTGGAAGAAta < 1:1002401/69‑2 (MQ=255) accaaccaCTGCTCTGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCGGTGAGTCTGGAAGAAta < 1:2166070/69‑2 (MQ=255) accaaccaCTGCTCTGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCGGTGAGTCTGGAAGAAta < 1:2003731/69‑2 (MQ=255) accaaccaCTGCTCTGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCGGTGAGTCTGGAAGAAta < 1:2589113/69‑2 (MQ=255) accaaccaCTGCTCTGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCGGTGAGTCTGGAAGAAta < 1:2768636/69‑2 (MQ=255) accaaccaCTGCTCTGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCGGTGAGTCTGGAAGAAta < 1:2853650/69‑2 (MQ=255) accaaccaCTGCTCTGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCGGTGAGTCTGGAAGAAta < 1:2855718/69‑2 (MQ=255) accaaccaCTGCTCTGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCGGTGAGTCTGGAAGAAta < 1:1809219/69‑2 (MQ=255) accaaccaCTGCTCTGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCGGTGAGTCTGGAAGAAta < 1:2917401/69‑2 (MQ=255) accaaccaCTGCTCTGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCGGTGAGTCTGGAAGAAta < 1:2975837/69‑2 (MQ=255) accaaccaCTGCTCTGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCGGTGAGTCTGGAAGAAta < 1:3194728/69‑2 (MQ=255) accaaccaCTGCTCTGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCGGTGAGTCTGGAAGAAta < 1:490115/69‑2 (MQ=255) accaaccaCTGCTCTGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCGGTGAGTCTGGAAGAAta < 1:1254750/69‑2 (MQ=255) ccAACCACTGCTCTGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCGGTGAGTCTGGAAGAAta < 1:1763570/68‑2 (MQ=255) ccAACCACTGCTCTGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCGGTGAGTCTGGAAGAAta < 1:2429672/68‑2 (MQ=255) ccACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGtcat < 1:1528940/69‑1 (MQ=255) ccACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGtcat < 1:1264403/69‑1 (MQ=255) gCTCTGACCACAAGTAATTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACtt > 1:1833913/1‑69 (MQ=255) ctctGACCACAAGTAATTGTTCAGATTGATAAAACGTGGc < 1:1432341/40‑1 (MQ=255) ctctGACCACAAGTAATTGTTCAGATTGATAAAACGTGGc < 1:2079556/40‑1 (MQ=255) ctctGACCACAAGTAATTGTTCAGATTGATAAAACGTGGc < 1:2318949/40‑1 (MQ=255) ctGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCg > 1:2431560/1‑39 (MQ=18) ctGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCGGTGAGTCTGGAAGAATAGtcatca > 1:3180355/1‑63 (MQ=19) ctGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCGGTGAGTCTGGAAGAATAGtcatca > 1:924416/1‑63 (MQ=19) ctGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCGGTGAGTCTGGAAGAATAGtcatca > 1:88859/1‑63 (MQ=19) ctGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCGGTGAGTCTGGAAGAATAGtcatca > 1:666062/1‑63 (MQ=19) ctGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCGGTGAGTCTGGAAGAATAGtcatca > 1:545916/1‑63 (MQ=19) ctGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCGGTGAGTCTGGAAGAATAGtcatca > 1:508192/1‑63 (MQ=19) ctGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCGGTGAGTCTGGAAGAATAGtcatca > 1:1258107/1‑63 (MQ=19) ctGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCGGTGAGTCTGGAAGAATAGtcatca > 1:423/1‑63 (MQ=19) ctGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCGGTGAGTCTGGAAGAATAGtcatca > 1:395572/1‑63 (MQ=19) ctGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCGGTGAGTCTGGAAGAATAGtcatca > 1:3295866/1‑63 (MQ=19) ctGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCGGTGAGTCTGGAAGAATAGtcatca > 1:136049/1‑63 (MQ=19) ctGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCGGTGAGTCTGGAAGAATAGtcatca > 1:3189399/1‑63 (MQ=19) ctGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCGGTGAGTCTGGAAGAATAGtcatca > 1:2203408/1‑63 (MQ=19) ctGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCGGTGAGTCTGGAAGAATAGtcatca > 1:3172654/1‑63 (MQ=19) ctGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCGGTGAGTCTGGAAGAATAGtcatca > 1:1615491/1‑63 (MQ=19) ctGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCGGTGAGTCTGGAAGAATAGtcatca > 1:1901592/1‑63 (MQ=19) ctGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCGGTGAGTCTGGAAGAATAGtcatca > 1:1908354/1‑63 (MQ=19) ctGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCGGTGAGTCTGGAAGAATAGtcatca > 1:2727952/1‑63 (MQ=19) ctGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCGGTGAGTCTGGAAGAATAGtcatca > 1:1916792/1‑63 (MQ=19) ctGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCGGTGAGTCTGGAAGAATAGtcatca > 1:2588595/1‑63 (MQ=19) ctGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCGGTGAGTCTGGAAGAATAGtcatca > 1:1931129/1‑63 (MQ=19) ctGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCGGTGAGTCTGGAAGAATAGtcatca > 1:1000458/1‑63 (MQ=19) ctGACCATAAGTAGTTGTTCAGATTGATAAAGCGTTGCGGTGAGTCTGGAAGAATAGtcatc > 1:920606/1‑62 (MQ=19) acaAGTAATTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCggg < 1:3397077/69‑1 (MQ=255) aaGTAATTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCggg < 1:1818901/67‑1 (MQ=255) tGATAAAACGTGGCGGTGAGTCCGGCAAAATCGtcat < 1:193841/37‑1 (MQ=255) aCGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCg > 1:3042297/1‑68 (MQ=255) aCGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCg > 1:1805621/1‑68 (MQ=255) aCGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCg > 1:3421974/1‑68 (MQ=255) | GAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAATCGTCATCACTTGCTCGCGGGCATAACAGACAAATTCCTGTAGCG > minE/252965‑253098 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |