| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,981,016 | C→T | 16.2% | E195E (GAG→GAA) | rob ← | DNA‑binding transcriptional activator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,981,016 | 0 | C | T | 16.2% | 91.6 / 7.1 | 55 | E195E (GAG→GAA) | rob | DNA‑binding transcriptional activator |
| Reads supporting (aligned to +/- strand): ref base C (21/25); new base T (6/3); total (27/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.95e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.74e-01 | |||||||||||
ACCGGATGCCCCGTCAGTACATAGCCATCTGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTAGAGCACCGGCGGAAT > minE/2980951‑2981081 | aCCGGATGCCCCGTCAGTACATAGCCATCTGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACctct > 1:461423/1‑69 (MQ=255) ccGTCAGTACATAGCCATCTGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACTTCTTGTtcgtcg < 1:1049678/68‑1 (MQ=255) ccGTCAGTACATAGCCATCTGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACTTCTTGTtcgtcg < 1:2835091/68‑1 (MQ=255) ccGTCAGTACATAGCCATCTGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACTTCTTGTtcgtcg < 1:1817934/68‑1 (MQ=255) aGTACATAGCCATCTGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTa < 1:2405859/69‑1 (MQ=255) tAGCCATCTGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGa > 1:2755874/1‑69 (MQ=255) ccATCTGCCTGATCGTGGGCTAACGCGGTGGTATAGAATACTTCTTGTTCGTCGTCTTTATCCTGACt > 1:410369/1‑68 (MQ=255) ccATCTGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACTTCTTgt > 1:2035147/1‑47 (MQ=255) ccATCTGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACTTCTTGTTCGTCGTCTTTATCCTGAc > 1:3417363/1‑67 (MQ=255) ccATCTGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACTTCTTGTTCGTCGTCTTTATCCTGACt > 1:100146/1‑68 (MQ=255) ccATCTGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACTTCTTGTTCGTCGTCTTTATCCTGACt > 1:2479734/1‑68 (MQ=255) ccATCTGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACTTCTTGTTCGTCGTCTTTATCCTGACt > 1:251903/1‑68 (MQ=255) cATCTGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCg < 1:2588809/69‑1 (MQ=255) tGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTcgtcgt < 1:1661205/50‑1 (MQ=255) tGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTcgtcgt < 1:225785/50‑1 (MQ=255) tGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTcgtcgt < 1:2201105/50‑1 (MQ=255) tGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTcgtcgt < 1:1665090/50‑1 (MQ=255) tGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTcgtcgt < 1:2857660/50‑1 (MQ=255) tGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTcgtcgt < 1:1618062/50‑1 (MQ=255) tGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTcgtcgt < 1:3027642/50‑1 (MQ=255) tGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTcgtcgt < 1:1165685/50‑1 (MQ=255) tGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTcgtcgt < 1:840207/50‑1 (MQ=255) tGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGAcg > 1:865011/1‑69 (MQ=255) tGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGAcg > 1:293876/1‑69 (MQ=255) tGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGAcg > 1:782296/1‑69 (MQ=255) tGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGAcg > 1:2394348/1‑69 (MQ=255) tGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGAc > 1:3174214/1‑68 (MQ=255) tGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGAc > 1:1564423/1‑68 (MQ=255) aaCGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTGATTCAgga > 1:1017225/1‑66 (MQ=255) aaCGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAgg > 1:1440780/1‑66 (MQ=255) ggtggtATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTagag < 1:3084633/69‑1 (MQ=255) ggtggtATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTagag < 1:2124200/69‑1 (MQ=255) ggtggtATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTagag < 1:2216779/69‑1 (MQ=255) gtggtATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTAGAGc < 1:2681892/69‑1 (MQ=255) taGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTagag < 1:2315141/62‑1 (MQ=255) taGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTAGAGCACcgg > 1:2819108/1‑68 (MQ=255) taGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTAGAGCACcgg > 1:1648647/1‑68 (MQ=255) gAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTAGAGCACcggcgg > 1:2165934/1‑69 (MQ=255) gAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTAGAGCACcggcgg < 1:1083605/69‑1 (MQ=255) gAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTAGAGCACcggcgg < 1:469987/69‑1 (MQ=255) gAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTAGAGCACcggcgg < 1:1218108/69‑1 (MQ=255) gAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTAGAGCACcggcgg > 1:1420091/1‑69 (MQ=255) gAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTAGAGCACcggcgg < 1:2293936/69‑1 (MQ=255) gAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTAGAGCACcggcgg < 1:3355215/69‑1 (MQ=255) gAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTAGAGCACcggcgg < 1:3267332/69‑1 (MQ=255) gAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTAGAGCACcggcgg < 1:1597935/69‑1 (MQ=255) gAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTAGAGCACcggcgg < 1:2861557/69‑1 (MQ=255) gAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTAGAGCACcggcgg < 1:194366/69‑1 (MQ=255) tACCTCTTGCTCGTCGTCTTTATCCTGAGTCGGACGCGTTTCATTCAGGCCGTAGAGCACCGGCGGAAt > 1:3300949/1‑69 (MQ=255) tACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTa > 1:412771/1‑54 (MQ=255) tACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTAGAGCACCGGCGGAAt > 1:3282462/1‑69 (MQ=255) tACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTAGAGCACCGGCGGAAt > 1:2288283/1‑69 (MQ=255) tACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTAGAGCACCGGCGGAAt > 1:2374075/1‑69 (MQ=255) tACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTAGAGCACCGGCGGAAt > 1:2995992/1‑69 (MQ=255) tACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTAGAGCACCGGCGGAAt > 1:518142/1‑69 (MQ=255) tACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTAGAGCACCGGCGGAAt > 1:1066093/1‑69 (MQ=255) | ACCGGATGCCCCGTCAGTACATAGCCATCTGCCTGATCCTGGGCTAACGCGGTGGTATAGAATACCTCTTGCTCGTCGTCTTTATCCTGACTCGGACGCGTTTCATTCAGGCCGTAGAGCACCGGCGGAAT > minE/2980951‑2981081 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |