| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 29,541 | G→A | 37.1% | E349E (GAG→GAA) | carB → | carbamoyl‑phosphate synthase large subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 29,541 | 0 | G | A | 37.1% | 43.5 / 41.2 | 70 | E349E (GAG→GAA) | carB | carbamoyl‑phosphate synthase large subunit |
| Reads supporting (aligned to +/- strand): ref base G (28/16); new base A (20/6); total (48/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.95e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.21e-01 | |||||||||||
GGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGTGCTA > minE/29479‑29605 | ggTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCGGCCTCCTTCGAGCCGTcc > 1:958270/1‑69 (MQ=255)ggTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCGGCCTCCTTCGAGCCGTcc > 1:29093/1‑69 (MQ=255) acacCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCGGCCTCCTTCGAGCCGTCCATc > 1:1362867/1‑68 (MQ=255) acacCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCGGCCTCCTTCGAGCCGTCCATCg > 1:3017048/1‑69 (MQ=255) cccTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCGGCCTCCTTCGAGCCGTCCATCGAc < 1:2778722/68‑1 (MQ=255) cgacgaACTGATGAACGACATCACTGGCGGACGTACTCCGGCCTCCTTCGAGCCGTCCATCGACTAtgt < 1:2656168/69‑1 (MQ=255) cgacgaACTGATGAACGACATCACTGGCGGACGTACTCCGGCCTCCTTCGAGCCGTCCATCGACTAtgt < 1:216505/69‑1 (MQ=255) gacgaACTGATGAACGACATCACTGGCGGACGTACTCCGGCCTCCTTCGAGCCGTCCATCGACTAtgtg > 1:2506297/1‑69 (MQ=255) gacgaACTGATGAACGACATCACTGGCGGACGTACTCCGGCCTCCTTCGAGCCGTCCATCGACTAtgtg > 1:857527/1‑69 (MQ=255) gacgaACTGATGAACGACATCACTGGCGGACGTACTCCGGCCTCCTTCGAGCCGTCCATCGACTAtgtg > 1:447672/1‑69 (MQ=255) gacgaACTGATGAACGACATCACTGGCGGACGTACTCCGGCCTCCTTCGAGCCGTCCATCGACTAtgtg > 1:3329972/1‑69 (MQ=255) gacgaACTGATGAACGACATCACTGGCGGACGTACTCCGGCCTCCTTCGAGCCGTCCATCGACTAtgtg > 1:2323438/1‑69 (MQ=255) cgaACTGATGAACGACATCACTGGCGGACGTACTCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGt < 1:3250412/69‑1 (MQ=255) tgatgaACGACATCACTGGCGGACGTACTCCGGCCTCCTTCGAGCCGTc > 1:3181724/1‑49 (MQ=255) tgatgaACGACATCACTGGCGGACGTACTCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTaga > 1:2303772/1‑66 (MQ=255) tgatgaACGACATCACTGGCGGACGTACTCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTaga > 1:1269988/1‑66 (MQ=255) tgatgaACGACATCACTGGCGGACGTACTCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTaga > 1:66043/1‑66 (MQ=255) tgatgaACGACATCACTGGCGGACGTACTCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTaga > 1:2126455/1‑66 (MQ=255) tgatgaACGACATCACTGGCGGACGTACTCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTaga > 1:2639633/1‑66 (MQ=255) tgatgaACGACATCACTGGCGGACGTACTCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTaga > 1:1791722/1‑66 (MQ=255) tgatgaACGACATCACTGGCGGACGTACTCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTaga > 1:3315627/1‑66 (MQ=255) tgatgaACGACATCACTGGCGGACGTACTCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTaga > 1:1670257/1‑66 (MQ=255) tgatgaACGACATCACTGGCGGACGTACTCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTaga > 1:1046580/1‑66 (MQ=255) tgatgaACGACATCACTGGCGGACGTACTCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTaga > 1:2948170/1‑66 (MQ=255) tgatgaACGACATCACTGGCGGACGTACTCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTaga > 1:145771/1‑66 (MQ=255) aTCACCGGCGGGCGTACTCCGGCCTCCTTCGAACCGTCCATCGAc < 1:2827487/45‑1 (MQ=255) aTCACCGGCGGGCGTACTCCGGCCTCCTTCGAACCGTCCATCGAc < 1:1209699/45‑1 (MQ=255) aTCACCGGCGGGCGTACTCCGGCCTCCTTCGAACCGTCCATCGAc < 1:2946652/45‑1 (MQ=255) aTCACCGGCGGGCGTACTCCGGCCTCCTTCGAACCGTCCATCGAc < 1:3417018/45‑1 (MQ=255) aTCACCGGCGGGCGTACTCCGGCCTCCTTCGAACCGTCCATCGAc < 1:2145553/45‑1 (MQ=255) aTCACCGGCGGGCGTACTCCGGCCTCCTTCGAACCGTCCATCGAc < 1:1833921/45‑1 (MQ=255) aCTGGCGGACGTACTCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTc < 1:1330558/69‑1 (MQ=255) aCTGGCGGACGTACTCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTc < 1:2830096/69‑1 (MQ=255) cTGGCGGTCGTACTCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTc < 1:1756940/68‑1 (MQ=255) tGGCGGACGTACTCCGGCCTCCTTCGAGCCGTCCATc > 1:1661818/1‑37 (MQ=255) tGGCGGACGTACTCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCaa > 1:274156/1‑69 (MQ=255) tGGCGGACGTACTCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCaa > 1:3037061/1‑69 (MQ=255) tGGCGGACGTACTCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCaa > 1:1264507/1‑69 (MQ=255) tGGCGGACGTACTCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCaa > 1:513768/1‑69 (MQ=255) tGGCGGACGTACTCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCaa > 1:52394/1‑69 (MQ=255) gTACTCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACt > 1:2188968/1‑63 (MQ=255) tcctTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGt < 1:2654374/69‑1 (MQ=255) tcctTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGt < 1:1841472/69‑1 (MQ=255) tcctTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGt < 1:2841808/69‑1 (MQ=255) tcctTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGt < 1:1200104/69‑1 (MQ=255) tcctTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGt < 1:2351007/69‑1 (MQ=255) tcctTCGAACCGTCCATCGACTATGTGGTCACCaa > 1:406914/1‑35 (MQ=25) tcctTCGAACCGTCCATCGACTATGTGGTCACCaa > 1:631432/1‑35 (MQ=25) tcctTCGAACCGTCCATCGACTATGTGGTCACCaa > 1:438247/1‑35 (MQ=25) tcctTCGAACCGTCCATCGACTATGTGGTCACCaa > 1:1056709/1‑35 (MQ=25) tcctTCGAACCGTCCATCGACTATGTGGTCACCaa > 1:1041531/1‑35 (MQ=25) tcctTCGAACCGTCCATCGACTATGTGGTCACCaa > 1:632825/1‑35 (MQ=25) tcctTCGAACCGTCCATCGACTATGTGGTCACCaa > 1:706359/1‑35 (MQ=25) tcctTCGAACCGTCCATCGACTATGTGGTCACCaa > 1:94265/1‑35 (MQ=25) tcctTCGAACCGTCCATCGACTATGTGGTCACCaa > 1:1397528/1‑35 (MQ=25) tcctTCGAACCGTCCATCGACTATGTGGTCACCaa > 1:37022/1‑35 (MQ=25) tcctTCGAACCGTCCATCGACTATGTGGTCACCaa > 1:1103166/1‑35 (MQ=25) tcctTCGAACCGTCCATCGACTATGTGGTCACCaa > 1:3265559/1‑35 (MQ=25) tcctTCGAACCGTCCATCGACTATGTGGTCACCaa > 1:1166681/1‑35 (MQ=25) tcctTCGAACCGTCCATCGACTATGTGGTCACCaa > 1:1016759/1‑35 (MQ=25) tcctTCGAACCGTCCATCGACTATGTGGTCACCaa > 1:2403008/1‑35 (MQ=25) tcctTCGAACCGTCCATCGACTATGTGGTCACCaa > 1:2180162/1‑35 (MQ=25) tcctTCGAACCGTCCATCGACTATGTGGTCACCaa > 1:2155675/1‑35 (MQ=25) tcctTCGAACCGTCCATCGACTATGTGGTCACCaa > 1:153862/1‑35 (MQ=25) tcctTCGAACCGTCCATCGACTATGTGGTCACCaa > 1:1531154/1‑35 (MQ=25) tcctTCGAACCGTCCATCGACTATGTGGTCACCaa > 1:1414671/1‑35 (MQ=25) tCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGTGCTa < 1:380389/69‑1 (MQ=255) tCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGTGCTa < 1:2439063/69‑1 (MQ=255) tCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGTGCTa < 1:1724690/69‑1 (MQ=255) tCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGTGCTa < 1:957301/69‑1 (MQ=255) | GGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCGGCCTCCTTCGAGCCGTCCATCGACTATGTGGTTACTAAAATTCCTCGCTTCAACTTCGAAAAATTCGCCGGTGCTA > minE/29479‑29605 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |