| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 256,007 | G→A | 47.7% | A84A (GCG→GCA) | yajC → | SecYEG protein translocase auxillary subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 256,007 | 0 | G | A | 47.7% | 15.6 / 114.0 | 132 | A84A (GCG→GCA) | yajC | SecYEG protein translocase auxillary subunit |
| Reads supporting (aligned to +/- strand): ref base G (32/37); new base A (51/12); total (83/49) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.90e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 136 total aligned reads displayed.
GAACGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACC > minE/255947‑256073 | gAACGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAAt < 1:20323/67‑1 (MQ=255) ggttggtCGCGTAACCAAAGTAGCTGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAagt < 1:1504089/69‑1 (MQ=255) ggttggtCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAagt < 1:3150/69‑1 (MQ=255) ggttggtCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAagt < 1:477976/69‑1 (MQ=255) gttggtCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAagta > 1:3129369/1‑69 (MQ=255) gttggtCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAagta > 1:3314253/1‑69 (MQ=255) gttggtCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAagta > 1:3003346/1‑69 (MQ=255) gttggtCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAagta > 1:2901482/1‑69 (MQ=255) cgcgTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCACTGAATGCCACCACTGAAGTAGttat > 1:3367437/1‑68 (MQ=255) cgcgTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCACTGAATGACACCACTGAAGTAGttat > 1:2345862/1‑68 (MQ=255) cgcgTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCACTGAATGACACCACTGAAGTAGttat > 1:2142618/1‑68 (MQ=255) cgcgTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCACTGAATGACACCACTGAAGTAGttat > 1:2135954/1‑68 (MQ=255) cgcgTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCACTGAATGACACCACTGAAGTAGttat > 1:3428978/1‑68 (MQ=255) cgcgTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCACTGAATGACACCACTGAAGTAGttat > 1:168970/1‑68 (MQ=255) cgcgTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCACTGAATGACACCACTGAAGTAGttat > 1:893060/1‑68 (MQ=255) cgcgTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCACTGAATGACACCACTGAAGTAGttat > 1:1325861/1‑68 (MQ=255) cgcgTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCACTGAATGACACCACTGAAGTAGttat > 1:2517368/1‑68 (MQ=255) cgcgTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCACTGAATGACACCACTGAAGTAGttat > 1:1290178/1‑68 (MQ=255) cgcgTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCACTGAATGACACCACTGAAGTAGttat > 1:1205712/1‑68 (MQ=255) cgcgTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGAACTGAATGACACCACTGAAGTAGttat > 1:3296317/1‑68 (MQ=255) gTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTaaa > 1:2299033/1‑69 (MQ=255) gTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTaaa > 1:965200/1‑69 (MQ=255) gTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTaaa > 1:2555083/1‑69 (MQ=255) gTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTaaa > 1:2367894/1‑69 (MQ=255) aCCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGt < 1:2890402/69‑1 (MQ=255) aCCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGt < 1:2333873/69‑1 (MQ=255) aCCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGt < 1:256401/69‑1 (MQ=255) aCCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGt > 1:3196165/1‑69 (MQ=255) aCCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGt > 1:1837971/1‑69 (MQ=255) aCCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGt > 1:410338/1‑69 (MQ=255) aCCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGt > 1:1670783/1‑69 (MQ=255) aCCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGt < 1:2867634/69‑1 (MQ=255) aCCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGt < 1:686274/69‑1 (MQ=255) aCCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGt < 1:1531252/69‑1 (MQ=255) aCCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGt > 1:580046/1‑69 (MQ=255) aCCAAAGTAGCGGAAAACGGCTACATTGCTATCGCACTGAATGACACCACTGAAGTAGTTATTAAACGt < 1:1083971/69‑1 (MQ=255) aCCAAAGTAGCGGAAAACGGCTACATTGCTATCGCACTGAATGACACCACTGAAGTAGTTATTAAACGt < 1:2847413/69‑1 (MQ=255) aCCAAAGTAGCGGAAAACGGCTACATTGCTATCGCACTGAATGACACCACTGAAGTAGTTATTAAACGt < 1:3205410/69‑1 (MQ=255) aCCAAAGTAGCGGAAAACGGCTACATTGCTATCGCACTGAATGACACCACTGAAGTAGTTATTAAACGt < 1:3268787/69‑1 (MQ=255) aCCAAAGTAGCGGAAAACGGCTACATTGCTATCGCACTGAATGACACCACTGAAGTAGTTATTAAACGt < 1:413310/69‑1 (MQ=255) aCCAAAGTAGCGGAAAACGGCTACATTGCTATCGCACTGAATGACACCACTGAAGTAGTTATTAAACGt < 1:1524684/69‑1 (MQ=255) aCCAAAGTAGCGGAAAACGGCTACATTGCTATCGCACTGAATGACACCACTGAAGTAGTTATTAAACGt < 1:151862/69‑1 (MQ=255) aCCAAAGTAGCGGAAAACGGCTACATTGCTATCGCACTGAATGACACCACTGAAGTAGTTATTAAACGt < 1:1314226/69‑1 (MQ=255) aCCAAAGTAGCGGAAAACGGCTACATTGCTATCGCACTGAATGACACCACTGAAGTAGTTATTAAACGt < 1:824942/69‑1 (MQ=255) aCCAAAGTAGCGGAAAACGGCTACATTGCTATCGCACTGAATGACACCACTGAAGTAGTTATTAAACGt < 1:856952/69‑1 (MQ=255) ccAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGt < 1:260387/68‑1 (MQ=255) tAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCg < 1:731249/69‑1 (MQ=255) tAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCg < 1:97996/69‑1 (MQ=255) tAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCg < 1:2795047/69‑1 (MQ=255) tAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCg < 1:1213976/69‑1 (MQ=255) tAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCg < 1:1278827/69‑1 (MQ=255) tAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCg < 1:1799608/69‑1 (MQ=255) tAGCGGAAAACGCCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCg < 1:893632/69‑1 (MQ=255) aTTGCTGTCGCACTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCt > 1:2024/1‑57 (MQ=255) aTTGCTATCGCACTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGc > 1:2000153/1‑56 (MQ=255) aTTGCTATCGCACTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCt > 1:2212181/1‑57 (MQ=255) aTTGCTATCGCACTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCt > 1:2713804/1‑57 (MQ=255) aTTGCTATCGCACTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCt > 1:2039068/1‑57 (MQ=255) aTTGCTATCGCACTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCt > 1:1932123/1‑57 (MQ=255) aTTGCTATCGCACTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCt > 1:3354694/1‑57 (MQ=255) aTTGCTATCGCACTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCt > 1:1818759/1‑57 (MQ=255) aTTGCTATCGCACTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCt > 1:497807/1‑57 (MQ=255) aTTGCTATCGCACTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCt > 1:538400/1‑57 (MQ=255) aTTGCTATCGCACTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCt > 1:607827/1‑57 (MQ=255) aTTGCTATCGCACTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCt > 1:1384112/1‑57 (MQ=255) aTTGCTATCGCACTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCt > 1:1340260/1‑57 (MQ=255) aTTGCTATCGCACTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCt > 1:869026/1‑57 (MQ=255) aTTGCTATCGCACTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCt > 1:2483607/1‑57 (MQ=255) aTTGCTATCGCACTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCt > 1:2796172/1‑57 (MQ=255) aTTGCTATCGCACTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCt > 1:2776143/1‑57 (MQ=255) aTTGCTATCGCACTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCt > 1:3096972/1‑57 (MQ=255) aTTGCTATCGCACTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCt > 1:2652913/1‑57 (MQ=255) aTTGCTATCGCACTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCt > 1:2461284/1‑57 (MQ=255) cTATCGCGCTGAATGACACCACTGATGTAGTTATTAAACGTGACTTCGTAGCTGCCGt > 1:314850/1‑58 (MQ=255) cTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCt < 1:1171402/61‑1 (MQ=255) cTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCt < 1:2723733/61‑1 (MQ=255) cTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCt > 1:1137060/1‑61 (MQ=255) cTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCt > 1:2608093/1‑61 (MQ=255) cTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCt < 1:2359240/61‑1 (MQ=255) cTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCt < 1:2807406/61‑1 (MQ=255) cTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCt > 1:1054800/1‑61 (MQ=255) cTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCt < 1:2542357/61‑1 (MQ=255) cTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCt > 1:1463425/1‑61 (MQ=255) cTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCt < 1:2865181/61‑1 (MQ=255) cTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCt > 1:2507242/1‑61 (MQ=255) cTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCt > 1:1584646/1‑61 (MQ=255) cTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCt > 1:430938/1‑61 (MQ=255) cTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCt < 1:1600975/61‑1 (MQ=255) cTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCt > 1:1768195/1‑61 (MQ=255) cTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCt < 1:248342/61‑1 (MQ=255) cTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCt < 1:2904785/61‑1 (MQ=255) cTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCt > 1:2937898/1‑61 (MQ=255) cTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCt < 1:330285/61‑1 (MQ=255) cTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCt > 1:1982782/1‑61 (MQ=255) cTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCt > 1:3242719/1‑61 (MQ=255) cTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCt < 1:3064710/61‑1 (MQ=255) cTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCt < 1:3188546/61‑1 (MQ=255) gcgcTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCAcc > 1:685119/1‑69 (MQ=255) gcgcTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCAcc > 1:832097/1‑69 (MQ=255) gcgcTGAATGACACCACTGAAGTAGTTATTAAACGTCACTTCGTAGCTGCCGTCCTGCCGAAAGGCAcc > 1:3109202/1‑69 (MQ=255) | GAACGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACC > minE/255947‑256073 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |