| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 260,171 | T→C | 43.4% | P104P (CCA→CCG) | tsx ← | nucleoside channel, receptor of phage T6 and colicin K |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 260,171 | 0 | T | C | 43.4% | 25.7 / 83.4 | 106 | P104P (CCA→CCG) | tsx | nucleoside channel, receptor of phage T6 and colicin K |
| Reads supporting (aligned to +/- strand): ref base T (26/34); new base C (11/35); total (37/69) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.23e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.18e-01 | |||||||||||
Only 100 of 106 total aligned reads displayed.
ACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATACCTTTAGCATCGGAGTT > minE/260110‑260230 | aCCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAACCGTGGTTCGa > 1:2494092/1‑69 (MQ=255)aCCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGTGGTTCGa > 1:1883875/1‑69 (MQ=255)aCCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGTGGTTCGa > 1:2330711/1‑69 (MQ=255)aCCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGTGGTTCGa > 1:2997326/1‑69 (MQ=255)aCCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGTGGTTCGa > 1:2956155/1‑69 (MQ=255)aCCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGTGGTTCGa > 1:2799711/1‑69 (MQ=255) ccACTCTTTGAACGGACCAAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGCGGTTCGAt < 1:2742251/69‑1 (MQ=255) ccACTCTTTGAACGGACCAAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGCGGTTCGAt < 1:203437/69‑1 (MQ=255) ccACTCTTTGAACGGACCAAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGCGGTTCGAt < 1:2053986/69‑1 (MQ=255) ccACTCTTTGAACGGACCAAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGCGGTTCGAt < 1:1921938/69‑1 (MQ=255) ccACTCTTTGAACGGACCAAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGCGGTTCGAt < 1:2407609/69‑1 (MQ=255) ccACTCTTTGAACGGACCAAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGCGGTTCGAt < 1:2583310/69‑1 (MQ=255) ccACTCTTTGAACGGACCAAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGCGGTTCGAt < 1:2702921/69‑1 (MQ=255) ccACTCTTTGAACGGACCAAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGCGGTTCGAt < 1:168918/69‑1 (MQ=255) ccACTCTTTGAACGGACCAAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGCGGTTCGAt < 1:430296/69‑1 (MQ=255) ccACTCTTTGAACGGACCAAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGCGGTTCGAt < 1:90608/69‑1 (MQ=255) ccACTCTTTGAACGGACCAAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGCGGTTCGAt < 1:617650/69‑1 (MQ=255) ccACTCTTTGAACGGACCAAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGCGGTTCGAt < 1:449847/69‑1 (MQ=255) ccACTCTTTGAACGGACCAAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGCGGTTCGAt < 1:371031/69‑1 (MQ=255) ccACTCTTTGAACGGACCAAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGCGGTTCGAt < 1:3288073/69‑1 (MQ=255) ccACTCTTTGAACGGACCAAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGCGGTTCGAt < 1:2915837/69‑1 (MQ=255) cACTCTTTGAACGGACCAAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGCGGTTCGAt < 1:388624/68‑1 (MQ=255) cACTCTTTGAACGGACCAAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGCGGTTCGAt < 1:519589/68‑1 (MQ=255) cACTCTTTGAACGGACCAAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGCGGTTCGAt < 1:1623907/68‑1 (MQ=255) cACTCTTTGAACGGACCAAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGCGGTTCGAt < 1:1980913/68‑1 (MQ=255) tctTTGAACGGACCAAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGCGGTTCGATTTc > 1:2576087/1‑68 (MQ=255) tctTTGAACGGACCAAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGCGGTTCGATTTc > 1:2838634/1‑68 (MQ=255) tctTTGAACGGACCAAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGCGGTTCGATTTc > 1:2324120/1‑68 (MQ=255) tctTTGAACGGACCAAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGCGGTTCGATTTc > 1:2262480/1‑68 (MQ=255) tctTTGAACGGACCAAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGCGGTTCGATTTc > 1:618893/1‑68 (MQ=255) tctTTGAACGGACCAAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGCGGTTCGATTTc > 1:9905/1‑68 (MQ=255) tctTTGAACGGACCAAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGCGGTTCGATTTc > 1:1066145/1‑68 (MQ=255) tctTTGAACGGACCAAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGCGGTTCGATTTc > 1:1212373/1‑68 (MQ=255) tctTTGAACGGACCAAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGCGGTTCGATTTc > 1:1354297/1‑68 (MQ=255) tctTTGAACGGACCAAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGCGGTTCGATTTc > 1:1783649/1‑68 (MQ=255) ctTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTcc > 1:897619/1‑68 (MQ=255) tCAGTATTGGTCAGCTTGTCGATGTAGAAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGt > 1:871635/1‑67 (MQ=255) cAGTATTGGTCAGCTTGTCGATGGAGAAACGCGGTTCGAt < 1:2050461/40‑1 (MQ=255) cAGTATTGGTCAGCTTGTCGATGGAGAAACGCGGTTCGAt < 1:1017944/40‑1 (MQ=255) cAGTATTGGTCAGCTTGTCGATGGAGAAACGCGGTTCGAt < 1:824200/40‑1 (MQ=255) cAGTATTGGTCAGCTTGTCGATGGAGAAACGCGGTTCGAt < 1:772859/40‑1 (MQ=255) cAGTATTGGTCAGCTTGTCGATGGAGAAACGCGGTTCGAt < 1:3349357/40‑1 (MQ=255) cAGTATTGGTCAGCTTGTCGATGGAGAAACGCGGTTCGAt < 1:3230803/40‑1 (MQ=255) cAGTATTGGTCAGCTTGTCGATGGAGAAACGCGGTTCGAt < 1:3228915/40‑1 (MQ=255) cAGTATTGGTCAGCTTGTCGATGGAGAAACGCGGTTCGAt < 1:3086235/40‑1 (MQ=255) cAGTATTGGTCAGCTTGTCGATGGAGAAACGCGGTTCGAt < 1:2922326/40‑1 (MQ=255) cAGTATTGGTCAGCTTGTCGATGGAGAAACGCGGTTCGAt < 1:2582887/40‑1 (MQ=255) cAGTATTGGTCAGCTTGTCGATGGAGAAACGCGGTTCGAt < 1:1999965/40‑1 (MQ=255) cAGTATTGGTCAGCTTGTCGATGGAGAAACGCGGTTCGAt < 1:1822967/40‑1 (MQ=255) aGTATTGGTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTCCATCAACAGCGGAGAACCGTGGTTCCa < 1:2904915/69‑1 (MQ=255) aGTATTGGTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTCCATAAACAGCg > 1:1294096/1‑53 (MQ=255) aGTATTGGTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTCCATAAACAGCg > 1:3435705/1‑53 (MQ=255) aGTATTGGTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTCCATAAACAGCg > 1:3280298/1‑53 (MQ=255) aGTATTGGTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTCCATAAACAGCg > 1:537786/1‑53 (MQ=255) aGTATTGGTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTCCATAAACAGCg > 1:115271/1‑53 (MQ=255) aGTATTGGTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTCCATAAACAGCg > 1:2416765/1‑53 (MQ=255) aGTATTGGTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTCCATAAACAGCg > 1:2249316/1‑53 (MQ=255) aGTATTGGTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTCCATAAACAGCg > 1:902695/1‑53 (MQ=255) aGTATTGGTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTCCATAAACAGCg > 1:2130064/1‑53 (MQ=255) aGTATTGGTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCa < 1:789673/69‑1 (MQ=255) aGTATTGGTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCa < 1:124394/69‑1 (MQ=255) aGTATTGGTCAGCTTGTCGATGGAGAAACGCGGTTCTAt < 1:3168145/39‑1 (MQ=37) aGTATTGGTCAGCTTGTCGATGGAGAAACGCGGTTCGAt < 1:2796242/39‑1 (MQ=255) tATTGGTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGa < 1:2929155/69‑1 (MQ=255) tATTGGTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGa < 1:2932896/69‑1 (MQ=255) tATTGGTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGa < 1:3044423/69‑1 (MQ=255) ttGGTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATa < 1:1472838/69‑1 (MQ=255) ttGGTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATa < 1:349724/69‑1 (MQ=255) ttGGTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATa < 1:1214687/69‑1 (MQ=255) ttGGTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATa < 1:1349274/69‑1 (MQ=255) ttGGTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATa < 1:264656/69‑1 (MQ=255) ttGGTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATa < 1:1840798/69‑1 (MQ=255) ttGGTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATa < 1:223395/69‑1 (MQ=255) ttGGTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATa < 1:142221/69‑1 (MQ=255) ttGGTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATa < 1:835181/69‑1 (MQ=255) tGGGCAGCTTGTCGATGGAGAAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATa < 1:679629/68‑1 (MQ=255) ggTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTc < 1:781704/36‑1 (MQ=255) ggTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTc < 1:945951/36‑1 (MQ=255) ggTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTc < 1:2046726/36‑1 (MQ=255) ggTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTc < 1:33042/36‑1 (MQ=255) ggTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTc < 1:2586328/36‑1 (MQ=255) ggTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTc < 1:326130/36‑1 (MQ=255) ggTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTc < 1:2358902/36‑1 (MQ=255) ggTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTc < 1:143074/36‑1 (MQ=255) ggTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTc < 1:3078010/36‑1 (MQ=255) ggTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATa < 1:1229231/67‑1 (MQ=255) ggTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATa > 1:1789574/1‑67 (MQ=255) ggTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATa < 1:202060/67‑1 (MQ=255) ggTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATa < 1:3160113/67‑1 (MQ=255) ggTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATa > 1:1868020/1‑67 (MQ=255) ggTCAGCTTGTCGATGGAGAAACCTGGTTCGATTTc < 1:1733255/36‑1 (MQ=255) ggTCAGCTTGTCGATGGAGAAACCTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATa < 1:1919593/67‑1 (MQ=255) tGTCGATGGAGAAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATACCTTTAGCAt > 1:700614/1‑69 (MQ=255) gATGGAGAAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATACCTTTAGCATCgg < 1:2464877/68‑1 (MQ=255) aCGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATACCTTTAGCATCGGAGtt > 1:2956129/1‑63 (MQ=255) aCGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATACCTTTAGCATCGGAGtt > 1:817076/1‑63 (MQ=255) aCGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATACCTTTAGCATCGGAGtt > 1:2883775/1‑63 (MQ=255) aCGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATACCTTTAGCATCGGAGtt > 1:1487907/1‑63 (MQ=255) aCGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATACCTTTAGCATCGGAGtt > 1:1542278/1‑63 (MQ=255) aCGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATACCTTTAGCATCGGAGtt > 1:1164003/1‑63 (MQ=255) | ACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATACCTTTAGCATCGGAGTT > minE/260110‑260230 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |