| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 30,714 | G→A | 62.8% | T740T (ACG→ACA) | carB → | carbamoyl‑phosphate synthase large subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 30,714 | 0 | G | A | 62.8% | 20.6 / 24.3 | 43 | T740T (ACG→ACA) | carB | carbamoyl‑phosphate synthase large subunit |
| Reads supporting (aligned to +/- strand): ref base G (9/7); new base A (20/7); total (29/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.16e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGGTCGGGCGATGGAAATCGTCTATGACGAAGCTGACCTGCGTCGCTACTTCCAGACGGCGGTCAGCGTGTCTAACGATGCGCCAGTGTTGCTGGACCACTTCCTCGATG > minE/30657‑30766 | cggTCGGGCGATGGAAATCGTCTATGACGAAGCTGACCTGCGTCGCTACTTCCAGACAGCGGTCAGc < 1:567940/67‑1 (MQ=255)cggTCGGGCGATGGAAATCGTCTATGACGAAGCTGACCTGCGTCGCTACTTCCAGACAGCGGTCAGc < 1:1995324/67‑1 (MQ=255)cggTCGGGCGATGGAAATCGTCTATGACGAAGCTGACCTGCGTCGCTACTTCCAGACAGCGGTCAGc < 1:1806892/67‑1 (MQ=255)cggTCGGGCGATGGAAATCGTCTATGACGAAGCTGACCTGCGTCGCTACTTCCAGACAGCGGTCAGc < 1:951719/67‑1 (MQ=255)cggTCGGGCGATGGAAATCGTCTATGACGAAGCTGACCTGCGTCGCTACTTCCAGACAGCGGTCAGc < 1:543686/67‑1 (MQ=255)cggTCGGGCGATGGAAATCGTCTATGACGAAGCTGACCTGCGTCGCTACTTCCAGACAGCGGTCAGc < 1:593919/67‑1 (MQ=255)cggTCGGGCGATGGAAATCGTCTATGACGAAGCTGACCTGCGTCGCTACTTCCAGACAGCGGTCAGc < 1:2300317/67‑1 (MQ=255) cGGGCGATGGAAATCGTCTATGACGAAGCTGACCTGCGTCGCTACTTCCAGACGGCGGTCAGCGTGTCt > 1:1302967/1‑69 (MQ=255) cGTCTATGACGAAGCTGACCTGCGTCGCTACTTCCAGACGGCGGTCAGCGTGTCTAACGATgcg > 1:358174/1‑64 (MQ=255) cGTCTATGACGAAGCTGACCTGCGTCGCTACTTCCAGACGGCGGTCAGCGTGTCTAACGATGCGCCAgt < 1:1661371/69‑1 (MQ=255) cGTCTATGACGAAGCTGACCTGCGTCGCTACTTCCAGACGGCGGTCAGCGTGTCTAACGATGCGCCAgt < 1:1281427/69‑1 (MQ=255) cGTCTATGACGAAGCTGACCTGCGTCGCTACTTCCAGACGGCGGTCAGCGTGTCTAACGATGCGCCAgt < 1:2183257/69‑1 (MQ=255) cGTCTATGACGAAGCTGACCTGCGTCGCTACTTCCAGACGGCGGTCAGCGTGTCTAACGATGCGCCAgt > 1:1850484/1‑69 (MQ=255) tATGACGAAGCTGACCTGCGTCGCTACTTCCAGACAGCGGTCAGCGTATCTAACGATGCGCCAGTGTTg > 1:2379544/1‑69 (MQ=255) tATGACGAAGCTGACCTGCGTCGCTACTTCCAGACAGCGGTCAGCGTATCTAACGATGCGCCAGTGTTg > 1:2736969/1‑69 (MQ=255) tATGACGAAGCTGACCTGCGTCGCTACTTCCAGACAGCGGTCAGCGTATCTAACGATGCGCCAGTGTTg > 1:2966717/1‑69 (MQ=255) tATGACGAAGCTGACCTGCGTCGCTACTTCCAGACAGCGGTCAGCGTATCTAACGATGCGCCAGTGTTg > 1:743646/1‑69 (MQ=255) tATGACGAAGCTGACCTGCGTCGCTACTTCCAGACAGCGGTCAGCGTATCTAACGATGCGCCAGTGTTg > 1:3126262/1‑69 (MQ=255) tATGACGAAGCTGACCTGCGTCGCTACTTCCAGACAGCGGTCAGCGTATCTAACGATGCGCCAGTGTTg > 1:3300237/1‑69 (MQ=255) tATGACGAAGCTGACCTGCGTCGCTACTTCCAGACAGCGGTCAGCGTATCTAACGATGCGCCAGTGTTg > 1:492192/1‑69 (MQ=255) tATGACGAAGCTGACCTGCGTCGCTACTTCCAGACAGCGGTCAGCGTATCTAACGATGCGCCAGTGTTg > 1:664437/1‑69 (MQ=255) tATGACGAAGCTGACCTGCGTCGCTACTTCCAGACAGCGGTCAGCGTATCTAACGATGCGCCAGTGTTg > 1:692319/1‑69 (MQ=255) tATGACGAAGCTGACCTGCGTCGCTACTTCCAGACAGCGGTCAGCGTATCTAACGATGCGCCAGTGTTg > 1:712643/1‑69 (MQ=255) tATGACGAAGCTGACCTGCGTCGCTACTTCCAGACAGCGGTCAGCGTATCTAACGATGCGCCAGTGTTg > 1:1160017/1‑69 (MQ=255) tATGACGAAGCTGACCTGCGTCGCTACTTCCAGACAGCGGTCAGCGTATCTAACGATGCGCCAGTGTTg > 1:1428752/1‑69 (MQ=255) tATGACGAAGCTGACCTGCGTCGCTACTTCCAGACAGCGGTCAGCGTATCTAACGATGCGCCAGTGTTg > 1:1549972/1‑69 (MQ=255) tATGACGAAGCTGACCTGCGTCGCTACTTCCAGACAGCGGTCAGCGTATCTAACGATGCGCCAGTGTTg > 1:1662074/1‑69 (MQ=255) tATGACGAAGCTGACCTGCGTCGCTACTTCCAGACAGCGGTCAGCGTATCTAACGATGCGCCAGTGTTg > 1:1737311/1‑69 (MQ=255) tATGACGAAGCTGACCTGCGTCGCTACTTCCAGACAGCGGTCAGCGTATCTAACGATGCGCCAGTGTTg > 1:1817094/1‑69 (MQ=255) tATGACGAAGCTGACCTGCGTCGCTACTTCCAGACAGCGGTCAGCGTATCTAACGATGCGCCAGTGTTg > 1:2108064/1‑69 (MQ=255) tATGACGAAGCTGACCTGCGTCGCTACTTCCAGACAGCGGTCAGCGTATCTAACGATGCGCCAGTGTTg > 1:798180/1‑69 (MQ=255) tATGACGAAGCTGACCTGCGTCGCTACTTCCAGACAGCGGTCAGCGTATCTAACGATGCGACAGTGTTg > 1:702354/1‑69 (MQ=255) tATGACGAAGCTGACCTGCGTCGCTACTTCCAGACAGAGGTCAGCGTATCTAACGATGCGCCAGTGTTg > 1:1960383/1‑69 (MQ=255) tGACGAAGCTGACCTGCGTCGCTACTTCCAGACGGCGGTCAGCGTGTCTAACGATGCGCCAGTGTTGc > 1:1963027/1‑68 (MQ=255) tGACGAAGCTGACCTGCGTCGCTACTTCCAGACGGCGGTCAGCGTGTCTAACGATGCGCCAGTGTTGc > 1:2176852/1‑68 (MQ=255) tGACGAAGCTGACCTGCGTCGCTACTTCCAGACGGCGGTCAGCGTGTCTAACGATGCGCCAGTGTTGc > 1:2842126/1‑68 (MQ=255) tGACGAAGCTGACCTGCGTCGCTACTTCCAGACGGCGGTCAGCGTGTCTAACGATGCGCCAGTGTTGc > 1:1259552/1‑68 (MQ=255) tGACGAAGCTGACCTGCGACGCTACTTCCAGACGGCGGTCAGCGTGTCTAACGATGCGCCAGTGTTGc > 1:97544/1‑68 (MQ=255) cTGCGTCGCTACTTCCATACGGCGGTCAGCGTGTCTAACGATGCGCCAGTGTTGCTGGACCACTTCCTc < 1:2756013/69‑1 (MQ=255) cTGCGTCGCTACTTCCAGACGGCGGTCAGCGTGTCTAACGATGCGCCAGTGTTGCTGGACCACTTCCTc < 1:2337473/69‑1 (MQ=255) cTGCGTCGCTACTTCCAGACGGCGGTCAGCGTGTCTAACGATGCGCCAGTGTTGCTGGACCACTTCCTc < 1:1785787/69‑1 (MQ=255) cTGCGTCGCTACTTCCAGACGGCGGTCAGCGTGTCTAACGATGCGCCAGTGTTGCTGGACCACTTCCTc < 1:1251050/69‑1 (MQ=255) tCGCTACTTCCAGACGGCGGTCAGCGTGTCTAACGATGCGCCAGTGTTGCTGGACCACTTCCTCGATg > 1:2344058/1‑68 (MQ=255) | CGGTCGGGCGATGGAAATCGTCTATGACGAAGCTGACCTGCGTCGCTACTTCCAGACGGCGGTCAGCGTGTCTAACGATGCGCCAGTGTTGCTGGACCACTTCCTCGATG > minE/30657‑30766 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |