| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 262,916 | C→T | 48.4% | R331R (CGC→CGT) | ribD → | fused diaminohydroxyphosphoribosylaminopyrimidin e deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 262,916 | 0 | C | T | 48.4% | 1.7 / 54.5 | 64 | R331R (CGC→CGT) | ribD | fused diaminohydroxyphosphoribosylaminopyrimidin e deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase |
| Reads supporting (aligned to +/- strand): ref base C (10/23); new base T (13/18); total (23/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.36e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGCGGGTTTAGTCGATGAGCTGATTGTCTATATCGCACCTAAACTATTAGGCAGCGACGCCCGCGGATTATGCACGCTGCCAGGGCTTGAGAAATTAGCCGACGCCCCCCAATTTAAATTCAAAGAGATAC > minE/262853‑262983 | ggCGGGTTTAGTCGATGAGCTGATTGTCTATATCGCACCTAAACTATTAGGCAGCGACGCCCGCGGAt < 1:606549/68‑1 (MQ=255)ggCGGGTTTAGTCGATGAGCTGATTGTCTATATCGCACCTAAACTATTAGGCAGCGACGCCCGCGGAt < 1:681237/68‑1 (MQ=255)ggCGGGTTTAGTCGATGAGCTGATTGTCTATATCGCACCTAAACTATTAGGCAGCGACGCCCGCGGAt < 1:805570/68‑1 (MQ=255) cGGGTTTAGTCGATGAGCTGATTGTCTATATCGCACCTAAACTATTAGGCAGCGACGCCCGCGGATTa > 1:79081/1‑68 (MQ=255) cGGGTTTAGTCGATGAGCTGATTGTCTATATCGCACCTAAACTATTAGGCAGCGACGCCCGCGGATTa > 1:909796/1‑68 (MQ=255) cGGGTTTAGTCGATGAGCTGATTGTCTATATCGCACCTAAACTATTAGGCAGCGACGCCCGCGGATTa > 1:2526757/1‑68 (MQ=255) gggTTTAGTCGATGAGCTGATTGTCTATATCGCACCTAAACTATTAGGCAGCGACGCCCGCGGATTATg < 1:2248675/69‑1 (MQ=255) gggTTTAGTCGATGAGCTGATTGTCTATATCGCACCTAAACTATTAGGCAGCGACGCCCGCGGATTATg < 1:681949/69‑1 (MQ=255) gggTTTAGTCGATGAGCTGATTGTCTATATCGCACCTAAACTATTAGGCAGCGACGCCCGCGGATTATg < 1:699120/69‑1 (MQ=255) gggTTTAGTCGATGAGCTGATTGTCTATATCGCACCTAAACTATTAGGCAGCGACGCCCGCGGATTATg < 1:459184/69‑1 (MQ=255) gggTTTAGTCGATGAGCTGATTGTCTATATCGCACCTAAACTATTAGGCAGCGACGCCCGCGGATTATg < 1:815978/69‑1 (MQ=255) gggTTTAGTCGATGAGCTGATTGTCTATATCGCACCTAAACTATTAGGCAGCGACGCCCGCGGATTATg < 1:882929/69‑1 (MQ=255) gggTTTAGTCGATGAGCTGATTGTCTATATCGCACCTAAACTATTAGGCAGCGACGCCCGCGGATTATg < 1:2682532/69‑1 (MQ=255) gggTTTAGTCGATGAGCTGATTGTCTATATCGCACCTAAACTATTAGGCAGCGACGCCCGCGGATTATg < 1:105933/69‑1 (MQ=255) gggTTTAGTCGATGAGCTGATTGTCTATATCGCACCTAAACTATTAGGCAGCGACGCCCGCGGATTATg < 1:1237110/69‑1 (MQ=255) gggTTTAGTCGATGAGCTGATTGTCTATATCGCACCTAAACTATTAGGCAGCGACGCCCGCGGATTATg < 1:1140111/69‑1 (MQ=255) ggTTTAGTCGATGAGCTGATTGTCTATATCGCACCTAAACTATTAGGCAGCGACGCCCGCGGATTATg < 1:1549726/68‑1 (MQ=255) tAGTCGATGAGCTGATTGTCTATATCGCACCTAAACTATTAGGCAGCGACGCCCGCGGAt > 1:3405524/1‑60 (MQ=255) tAGTCGATGAGCTGATTGTCTATATCGCACCTAAACTATTAGGCAGCGACGCCCGCGGAt > 1:651628/1‑60 (MQ=255) tAGTCGATGAGCTGATTGTCTATATCGCACCTAAACTATTAGGCAGCGACGCCCGCGGAt > 1:2053816/1‑60 (MQ=255) tAGTCGATGAGCTGATTGTCTATATCGCACCTAAACTATTAGGCAGCGACGCCCGCGGAt > 1:1178411/1‑60 (MQ=255) tAGTCGATGAGCTGATTGTCTATATCGCACCTAAACTATTAGGCAGCGACGCCCGCGGAt > 1:3344504/1‑60 (MQ=255) gCACCTAAACTATTAGGCAGCGACGCCCGCGGATTATg < 1:1789541/38‑1 (MQ=255) ccTAAACTATTAGGCAACGACGCCCGTGTATTATGCACGCTGCCAGGGCTTGAGAAATTAGCCGACGct > 1:2246704/1‑68 (MQ=255) ccTAAACTATTAGGCAACGACGCCCGTGGATTATGCa > 1:2352902/1‑37 (MQ=255) ccTAAACTATTAGGCAACGACGCCCGTGGATTATGCACGCTGCCAGGGCTTGAGAAATTAGCCGACGct > 1:2406096/1‑68 (MQ=255) ccTAAACTATTAGGCAACGACGCCCGTGGATTATGCACGCTGCCAGGGCTTGAGAAATTAGCCGACGct > 1:2953138/1‑68 (MQ=255) ccTAAACTATTAGGCAACGACGCCCGTGGATTATGCACGCTGCCAGGGCTTGAGAAATTAGCCGACGct > 1:2968688/1‑68 (MQ=255) ccTAAACTATTAGGCAACGACGCCCGTGGATTATGCACGCTGCCAGGGCTTGAGAAATTAGCCGACGct > 1:180823/1‑68 (MQ=255) ccTAAACTATTAGGCAACGACGCCCGTGGATTATGCACGCTGCCAGGGCTTGAGAAATTAGCCGACGct > 1:167040/1‑68 (MQ=255) ccTAAACTATTAGGCAACGACGCCCGTGGATTATGCACGCTGCCAGGGCTTGAGAAATTAGCCGACGct > 1:1555087/1‑68 (MQ=255) ccTAAACTATTAGGCAACGACGCCCGTGGATTATGCACGCTGCCAGGGCTTGAGAAATTAGCCGACGct > 1:805775/1‑68 (MQ=255) ccTAAACTATTAGGCAACGACGCCCGTGGATTATGCACGCTGCCAGGGCTTGAGAAATTAGCCGACGct > 1:947957/1‑68 (MQ=255) ccTAAACTATTAGGCAACGACGCCCGTGGATTATGCACGCTGCCAGGGCTTGAGAAATTAGCCGACGct > 1:98519/1‑68 (MQ=255) ccTAAACTATTAGGCAACGACGCCCGTGGATTATGCACGCTGCCAGGGCTTGAGAAATTAGCCGACGc > 1:1275396/1‑68 (MQ=255) ccTAAACTATTAGGCAACGACGCCCGTGGATTATGCACGCTGACAGGGCTTGAGAAATTAGCCGACGct > 1:3176691/1‑68 (MQ=255) tAAACTATTAGGCACCGACGCCCGTGGATTATGCACGCTGCCAGGGCTTgag < 1:394024/52‑1 (MQ=39) tAAACTATTAGGCAACGACGCCCGTGGATTATGCACGCTGCCAGGGCTTgag < 1:639252/52‑1 (MQ=255) tAAACTATTAGGCAACGACGCCCGTGGATTATGCACGCTGCCAGGGCTTgag < 1:661170/52‑1 (MQ=255) tAAACTATTAGGCAACGACGCCCGTGGATTATGCACGCTGCCAGGGCTTgag < 1:59789/52‑1 (MQ=255) tAAACTATTAGGCAACGACGCCCGTGGATTATGCACGCTGCCAGGGCTTgag < 1:3120997/52‑1 (MQ=255) tAAACTATTAGGCAACGACGCCCGTGGATTATGCACGCTGCCAGGGCTTgag < 1:2600578/52‑1 (MQ=255) tAAACTATTAGGCAACGACGCCCGTGGATTATGCACGCTGCCAGGGCTTgag < 1:2838953/52‑1 (MQ=255) tAAACTATTAGGCAACGACGCCCGTGGATTATGCACGCTGCCAGGGCTTgag < 1:130319/52‑1 (MQ=255) tAAACTATTAGGCAACGACGCCCGTGGATTATGCACGCTGCCAGGGCTTgag < 1:1349645/52‑1 (MQ=255) tAAACTATTAGGCAACGACGCCCGTGGATTATGCACGCTGCCAGGGCTTgag < 1:1419664/52‑1 (MQ=255) tAAACTATTAGGCAACGACGCCCGTGGATTATGCACGCTGCCAGGGCTTgag < 1:1466425/52‑1 (MQ=255) tAAACTATTAGGCAACGACGCCCGTGGATTATGCACGCTGCCAGGGCTTgag < 1:1961004/52‑1 (MQ=255) tAAACTATTAGGCAACGACGCCCGTGGATTATGCACGCTGCCAGGGCTTgag < 1:214493/52‑1 (MQ=255) tAAACTATTAGGCAACGACGCCCGTGGATTATGCACGCTGCCAGGGCTTgag < 1:2159928/52‑1 (MQ=255) tAAACTATTAGGCAACGACGCCCGTGGATTATGCACGCTGCCAGGGCTTgag < 1:2210993/52‑1 (MQ=255) tAAACTATTAGGCAACGACGCCCGTGGATTATGCACGCTGCCAGGGCTTgag < 1:1023297/52‑1 (MQ=255) tAAACTATTAGGAAACGACGCCCGTGGATTATGCACGCTGCCAGGGCTTgag < 1:1924370/52‑1 (MQ=255) cTATTAGGCAGCGACGCCCGCGGATTATGCACGCTGCCAGGGCTTGAGAAATTAGCCGACGCCCCCCa < 1:2469397/68‑1 (MQ=255) cTATTAGGCAGCGACGCCCGCGGATTATGCACGCTGCCAGGGCTTGAGAAATTAGCCGACGCCCCCCa < 1:657702/68‑1 (MQ=255) cTATTAGGCAGCGACGCCCGCGGATTATGCACGCTGCCAGGGCTTGAGAAATTAGCCGACGCCCCCCa < 1:1590534/68‑1 (MQ=255) ttAGGCAACGACGCCCGTGGATTATGCACGCTGCCAGGGCTTgag < 1:2267179/45‑1 (MQ=255) gCAGCGACGCCCGCGGATTATGCACGCTGCCAGGGCTTGAGAAATTAGCCGACGCCCCCCAATTTaaa < 1:249865/68‑1 (MQ=255) gCAGCGACGCCCGCGGATTATGCACGCTGCCAGGGCTTGAGAAATTAGCCGACGCCCCCCAATTTaaa < 1:854961/68‑1 (MQ=255) gCAGCGACGCCCGCGGATTATGCACGCTGCCAGGGCTTGAGAAATTAGCCGACGCCCCCCAATTTaaa < 1:2933968/68‑1 (MQ=255) ccgCGACGCCCGCGGATTATGCACGCTGCCAGGGCTTGAGAAATTAGCCGACGCCCCCCAATTTaaa < 1:1890739/65‑1 (MQ=255) gCGACGCCCGCGGATTATGCACGCTGCCAGGGCTTGAGAAATTAgccgccgcc > 1:263577/1‑53 (MQ=255) gCGACGCCCGCGGATTATGCACGCTGCCAGGGCTTGAGAAATTAGCCGACGCCCCCCAATTTAAAt > 1:1995595/1‑66 (MQ=255) cccGCGGATTATGCACGCTGCCAGGGCTTGAGAAATTAGCCGACGCCCCCCAATTTAAATTCAAAgaga < 1:356625/69‑1 (MQ=255) gcgGATTATGCACGCTGCCAGGGCTTGAGAAATTAGCCGACGCCCCCCAATTTaaa < 1:940075/56‑1 (MQ=255) cgGATTATGCACGCTGCCAGGGCTTGAGAAATTAGCCGACGCCCCCCAATTTAAATTCAAAGAGATAc > 1:1028870/1‑68 (MQ=255) cgGATTATGCACGCTGCCAGGGCTTGAGAAATTAGCCGACGCCCCCCAATTTAAATTCAAAGAGATAc > 1:2639067/1‑68 (MQ=255) cgGATTATGCACGCTGCCAGGGCTTGAGAAATTAGCCGACGCCCCCCAATTTAAATTCAAAGAGATAc > 1:140197/1‑68 (MQ=255) cgGATTATGCACGCTGCCAGGGCTTGAGAAATTAGCCGACGCCCCCCAATTTAAATTCAAAGAGATAc > 1:642864/1‑68 (MQ=255) | GGCGGGTTTAGTCGATGAGCTGATTGTCTATATCGCACCTAAACTATTAGGCAGCGACGCCCGCGGATTATGCACGCTGCCAGGGCTTGAGAAATTAGCCGACGCCCCCCAATTTAAATTCAAAGAGATAC > minE/262853‑262983 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |