| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 265,813 | T→C | 43.5% | T264T (ACA→ACG) | yajO ← | predicted oxidoreductase, NAD(P)‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 265,813 | 0 | T | C | 43.5% | 13.0 / 53.4 | 69 | T264T (ACA→ACG) | yajO | predicted oxidoreductase, NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base T (8/31); new base C (15/15); total (23/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.94e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.18e-01 | |||||||||||
ATAATCGGTGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCT > minE/265754‑265878 | aTAATCGGTGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGCGTTGcacc < 1:2136436/68‑1 (MQ=255)aTAATCGGTGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGCGTTGcacc < 1:2651486/68‑1 (MQ=255)aTAATCGGTGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGCGTTGcacc < 1:1633965/68‑1 (MQ=255)aTAATCGGTGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGCGTTGcacc < 1:154311/68‑1 (MQ=255)aTAATCGGTGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGCGTTGcacc < 1:407491/68‑1 (MQ=255)aTAATCGGTGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGCGTTGcacc < 1:1442786/68‑1 (MQ=255)aTAATCGGTGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGCGTTGcacc < 1:1384770/68‑1 (MQ=255)aTAATCGGTGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGCGTTGcacc < 1:1365941/68‑1 (MQ=255)aTAATCGGTGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGCGTTGcacc < 1:1334657/68‑1 (MQ=255)aTAATCGGTGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGCGTTGcacc < 1:2475554/68‑1 (MQ=255)aTAATCGGTGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGCGTTGcacc < 1:486675/68‑1 (MQ=255)aTAATCGGTGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGCGTTGcacc < 1:187981/68‑1 (MQ=255)aTAATCGGTGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGCGTTGcacc < 1:750959/68‑1 (MQ=255)aTAATCGGTGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGCGTTGcacc < 1:1124042/68‑1 (MQ=255)aTAATCGGTGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGCGTTGcacc < 1:2173716/68‑1 (MQ=255) aaTCGGTGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAg < 1:1580645/69‑1 (MQ=255) aaTCGGTGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAg < 1:2857149/69‑1 (MQ=255) tGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGttctt < 1:2480885/68‑1 (MQ=255) tGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGttctt < 1:3260066/68‑1 (MQ=255) tGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGttctt < 1:2495094/68‑1 (MQ=255) tGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGttctt < 1:2522871/68‑1 (MQ=255) tGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGttctt < 1:2476848/68‑1 (MQ=255) tGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGttctt < 1:454356/68‑1 (MQ=255) tGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGttctt < 1:630933/68‑1 (MQ=255) tGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGttctt < 1:876089/68‑1 (MQ=255) tGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGttctt < 1:983109/68‑1 (MQ=255) cccGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCt > 1:405690/1‑69 (MQ=255) cccGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCt > 1:3886/1‑69 (MQ=255) cccGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCt > 1:2453729/1‑69 (MQ=255) cccGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCt > 1:1027170/1‑69 (MQ=255) cccGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCt > 1:567365/1‑69 (MQ=255) cccGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCt > 1:1257236/1‑69 (MQ=255) ttACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTaa > 1:3245279/1‑68 (MQ=255) ttACTCAACAA‑CAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAAcc > 1:755618/1‑69 (MQ=255) aacaacCAGGCCAGCGCAACTTGTGCTCGCGTTGCATCAAGttct > 1:2681150/1‑45 (MQ=255) aacaacCAGGCCAGCGCAACTTGTGCTCGCGTTGCATCAAGttct > 1:1524693/1‑45 (MQ=255) aacaacCAGGCCAGCGCAACTTGTGCTCGCGTTGCATCAAGttct > 1:1323877/1‑45 (MQ=255) aacaacCAGGCCAGCGCAACTTGTGCTCGCGTTGCATCAAGttct > 1:480839/1‑45 (MQ=255) aacaacCAGGCCAGCGCAACTTGTGCTCGCGTTGCATCAAGttct > 1:3219782/1‑45 (MQ=255) aacaacCAGGCCAGCGCAACTTGTGCTCGCGTTGCATCAAGttct > 1:283708/1‑45 (MQ=255) aacaacCAGGCCAGCGCAACTTGTGCTCGCGTTGCATCAAGttct > 1:2788436/1‑45 (MQ=255) aacaacCAGGCCAGCGCAACTTGTGCTCGCGTTGCATCAAGttct > 1:2513326/1‑45 (MQ=255) aacaacCAGGCCAGCGCAACTTGTGCTCGCGTTGCATCAAGttct > 1:1802764/1‑45 (MQ=255) aacaacCAGGCCAGCGCAACTTGTGCTCGCGTTGCATCAAGttct > 1:778526/1‑45 (MQ=255) aacaacCAGGCCAGCGCAACTTGTGCTCGCGTTGCATCAAGttct > 1:1820374/1‑45 (MQ=255) aacaacCAGGCCAGCGCAACTTGTGCTCGCGTTGCATCAAGttct > 1:1088274/1‑45 (MQ=255) aacaacCAGGCCAGCGCAACTTGTGCTCGCGTTGCATCAAGttct > 1:2153167/1‑45 (MQ=255) aacaacCAGGCCAGCGCAACTTGTGCTCGCGTTGCATCAAGttct > 1:1949803/1‑45 (MQ=255) aacaacCAGGCCAGCGCAACTTCTGCTCGCGTTGCATCAAGttct > 1:1945240/1‑45 (MQ=255) acCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGa < 1:1659871/69‑1 (MQ=255) acCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGa < 1:1268716/69‑1 (MQ=255) gcAACTTTTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCa < 1:1084224/69‑1 (MQ=255) gcAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCa < 1:3074650/69‑1 (MQ=255) gcAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCa < 1:1155640/69‑1 (MQ=255) gcAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCa < 1:902357/69‑1 (MQ=255) gcAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCa < 1:413182/69‑1 (MQ=255) gcAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCa < 1:3295611/69‑1 (MQ=255) gcAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCa < 1:1533356/69‑1 (MQ=255) gcAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCa < 1:3231949/69‑1 (MQ=255) gcAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCa < 1:2018486/69‑1 (MQ=255) gcAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCa < 1:3059982/69‑1 (MQ=255) gcAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCa < 1:174085/69‑1 (MQ=255) gcAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCa < 1:2484990/69‑1 (MQ=255) gcAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCa < 1:2061074/69‑1 (MQ=255) cAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCa < 1:2915906/68‑1 (MQ=255) cAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCa < 1:1849874/68‑1 (MQ=255) cAACTTGTGCTCGTGACGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCa < 1:1787698/68‑1 (MQ=255) cTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCa < 1:2219348/65‑1 (MQ=255) cGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGct < 1:687059/68‑1 (MQ=255) | ATAATCGGTGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCT > minE/265754‑265878 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |