| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 265,981 | T→C | 34.1% | V208V (GTA→GTG) | yajO ← | predicted oxidoreductase, NAD(P)‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 265,981 | 0 | T | C | 34.1% | 23.4 / 18.2 | 38 | V208V (GTA→GTG) | yajO | predicted oxidoreductase, NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base T (4/21); new base C (4/9); total (8/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.07e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGT > minE/265931‑266048 | tctcCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTATCGcc < 1:324846/57‑1 (MQ=255)tctcCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGcc < 1:993778/57‑1 (MQ=255)tctcCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGcc < 1:2732855/57‑1 (MQ=255)tctcCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGcc < 1:2358468/57‑1 (MQ=255)tctcCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGcc < 1:2892811/57‑1 (MQ=255)tctcCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGcc < 1:3190173/57‑1 (MQ=255)tctcCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGcc < 1:3240816/57‑1 (MQ=255)tctcCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGcc < 1:368891/57‑1 (MQ=255)tctcCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGcc < 1:1674264/57‑1 (MQ=255)tctcCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGcc < 1:1483355/57‑1 (MQ=255)tctcCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGcc < 1:948377/57‑1 (MQ=255)tctcCCCACGGACGCGTCAGACGGCCCCTT‑CCAGCGGGCTCCATGGAATTACCGcc < 1:2792452/56‑1 (MQ=255) caaggACGCGTCAGACGGCCCCGCGCCAGCGGTCTCCATGGAATCACCGCCACGCCTTCCTGATaa < 1:757872/63‑1 (MQ=255) caaggACGCGTCAGACGGCCCCGCGCCAGCGGGCTCCATGGAATCACCGCCACGCCTTCCTGATaa < 1:1694372/63‑1 (MQ=255) caaggACGCGTCAGACGGCCCCGCGCCAGCGGGCTCCATGGAATCACCGCCACGCCTTCCTGATaa < 1:2048167/63‑1 (MQ=255) caaggACGCGTCAGACGGCCCCGCGCCAGCGGGCTCCATGGAATCACCGCCACGCCTTCCTGATaa < 1:2115977/63‑1 (MQ=255) caaggACGCGTCAGACGGCCCCGCGCCAGCGGGCTCCATGGAATCACCGCCACGCCTTCCTGATaa < 1:1153374/63‑1 (MQ=255) caaggACGCGTCAGACGGCCCCGCGCCAGCGGGCTCCATGGAATCACCGCCACGCCTTCCTGATaa < 1:2542732/63‑1 (MQ=255) caaggACGCGTCAGACGGCCCCGCGCCAGCGGGCTCCATGGAATCACCGCCACGCCTTCCTGATaa < 1:2791567/63‑1 (MQ=255) cACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAacac < 1:409120/69‑1 (MQ=255) cACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAacac < 1:1103315/69‑1 (MQ=255) cACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAacac < 1:2001016/69‑1 (MQ=255) cACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAacac < 1:1099126/69‑1 (MQ=255) cACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAacac < 1:1192318/69‑1 (MQ=255) cACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAacac < 1:1588135/69‑1 (MQ=255) cACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAacac < 1:2378887/69‑1 (MQ=255) aggACGCGTCAGACGGCCCCGCGCCAGCGGGCTCCATGGAATCACCGCCACGCCTTCCTGATaa < 1:980858/63‑1 (MQ=255) cgCCAGCGGGCTCCATGGAATCACCGCCACGCCTTCCTGATaa < 1:2769459/42‑1 (MQ=37) gCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCtcttc > 1:2298865/1‑68 (MQ=255) gCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCtcttc > 1:2060850/1‑68 (MQ=255) gCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCtcttc > 1:687475/1‑68 (MQ=255) gCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCtcttc > 1:2707222/1‑68 (MQ=255) gggCTCCATGGAATCACCGCCACGCCTTCCTGATAACACAGCGGCAGCATCTCGCGCTCTTCTTCAc > 1:3422031/1‑67 (MQ=255) gggCTCCATGGAATCACCGCCACGCCTTCCTGATAACACAGCGGCAGCATCTCGCGCTCTTCTTCACg > 1:233112/1‑68 (MQ=255) gggCTCCATGGAATCACCGCCACGCCTTCCTGATAACACAGCGGCAGCATCTCGCGCTCTTCTTCACg > 1:2726780/1‑68 (MQ=255) gggCTCCATGGAATCACCGCCACGCCTTCCTGATAACACAGCGGCAGCATCTCGCGCTCTTCTTCACg > 1:828419/1‑68 (MQ=255) gCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATa < 1:1297044/69‑1 (MQ=255) gCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATa < 1:98858/69‑1 (MQ=255) ttACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGt < 1:2341921/69‑1 (MQ=255) | TCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGT > minE/265931‑266048 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |