| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 265,993 | C→T | 31.6% | E204E (GAG→GAA) | yajO ← | predicted oxidoreductase, NAD(P)‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 265,993 | 0 | C | T | 31.6% | 33.4 / 16.9 | 41 | E204E (GAG→GAA) | yajO | predicted oxidoreductase, NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base C (7/21); new base T (4/9); total (11/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.19e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCA > minE/265937‑266060 | caaggACGCGTCAGACGGCCCCGCGCCAGCGGTCTCCATGGAATCACCGCCACGCCTTCCTGATaa < 1:757872/63‑1 (MQ=255)caaggACGCGTCAGACGGCCCCGCGCCAGCGGGCTCCATGGAATCACCGCCACGCCTTCCTGATaa < 1:1153374/63‑1 (MQ=255)caaggACGCGTCAGACGGCCCCGCGCCAGCGGGCTCCATGGAATCACCGCCACGCCTTCCTGATaa < 1:2791567/63‑1 (MQ=255)caaggACGCGTCAGACGGCCCCGCGCCAGCGGGCTCCATGGAATCACCGCCACGCCTTCCTGATaa < 1:2542732/63‑1 (MQ=255)caaggACGCGTCAGACGGCCCCGCGCCAGCGGGCTCCATGGAATCACCGCCACGCCTTCCTGATaa < 1:2115977/63‑1 (MQ=255)caaggACGCGTCAGACGGCCCCGCGCCAGCGGGCTCCATGGAATCACCGCCACGCCTTCCTGATaa < 1:2048167/63‑1 (MQ=255)caaggACGCGTCAGACGGCCCCGCGCCAGCGGGCTCCATGGAATCACCGCCACGCCTTCCTGATaa < 1:1694372/63‑1 (MQ=255)cACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAacac < 1:1103315/69‑1 (MQ=255)cACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAacac < 1:409120/69‑1 (MQ=255)cACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAacac < 1:2378887/69‑1 (MQ=255)cACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAacac < 1:2001016/69‑1 (MQ=255)cACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAacac < 1:1588135/69‑1 (MQ=255)cACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAacac < 1:1099126/69‑1 (MQ=255)cACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAacac < 1:1192318/69‑1 (MQ=255) aggACGCGTCAGACGGCCCCGCGCCAGCGGGCTCCATGGAATCACCGCCACGCCTTCCTGATaa < 1:980858/63‑1 (MQ=255) cgCCAGCGGGCTCCATGGAATCACCGCCACGCCTTCCTGATaa < 1:2769459/42‑1 (MQ=37) gCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCtcttc > 1:687475/1‑68 (MQ=255) gCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCtcttc > 1:2060850/1‑68 (MQ=255) gCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCtcttc > 1:2298865/1‑68 (MQ=255) gCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCtcttc > 1:2707222/1‑68 (MQ=255) gggCTCCATGGAATCACCGCCACGCCTTCCTGATAACACAGCGGCAGCATCTCGCGCTCTTCTTCAc > 1:3422031/1‑67 (MQ=255) gggCTCCATGGAATCACCGCCACGCCTTCCTGATAACACAGCGGCAGCATCTCGCGCTCTTCTTCACg > 1:828419/1‑68 (MQ=255) gggCTCCATGGAATCACCGCCACGCCTTCCTGATAACACAGCGGCAGCATCTCGCGCTCTTCTTCACg > 1:2726780/1‑68 (MQ=255) gggCTCCATGGAATCACCGCCACGCCTTCCTGATAACACAGCGGCAGCATCTCGCGCTCTTCTTCACg > 1:233112/1‑68 (MQ=255) gCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATa < 1:98858/69‑1 (MQ=255) gCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATa < 1:1297044/69‑1 (MQ=255) ttACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGt < 1:2341921/69‑1 (MQ=255) aCCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTa > 1:1572527/1‑68 (MQ=255) aCCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTa > 1:344559/1‑68 (MQ=255) aCCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTa > 1:3329430/1‑68 (MQ=255) ccACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTTGTGAt < 1:1634480/69‑1 (MQ=255) ccACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGAt < 1:2503772/69‑1 (MQ=255) ccACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGAt < 1:914128/69‑1 (MQ=255) ccACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGAt < 1:1508873/69‑1 (MQ=255) ccACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGAt < 1:505637/69‑1 (MQ=255) ccACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGAt < 1:1399006/69‑1 (MQ=255) ccACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGAt < 1:3015885/69‑1 (MQ=255) ccACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGAt < 1:2772376/69‑1 (MQ=255) ccACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGAt < 1:2666493/69‑1 (MQ=255) ccACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGAt < 1:2543080/69‑1 (MQ=255) ccACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGAt < 1:1256745/69‑1 (MQ=255) cctcctGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCa < 1:3046768/69‑1 (MQ=255) cctcctGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCa < 1:1308351/69‑1 (MQ=255) cctcctGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCa < 1:1167385/69‑1 (MQ=255) cctcctGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCa < 1:1297253/69‑1 (MQ=255) cctcctGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCa < 1:594289/69‑1 (MQ=255) cctcctGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCa < 1:1965067/69‑1 (MQ=255) cctcctGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCa < 1:2183928/69‑1 (MQ=255) cctcctGATAACACAGTGCTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCa < 1:3075212/69‑1 (MQ=255) ctcctGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCa < 1:1692710/68‑1 (MQ=255) | CACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCA > minE/265937‑266060 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |