| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 30,808 | A→G | 100% | M772V (ATG→GTG) | carB → | carbamoyl‑phosphate synthase large subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 30,808 | 0 | A | G | 79.2% | 89.7 / 13.8 | 62 | M772V (ATG→GTG) | carB | carbamoyl‑phosphate synthase large subunit |
| Reads supporting (aligned to +/- strand): ref base A (9/4); new base G (0/49); total (9/53) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.52e-08 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GGACCACTTCCTCGATGACGCGGTAGAAGTTGACGTGGATGCCATCTGCGACGGCGAAATGGTGCTGATTGGCGGCATCATGGAGCATATTGAGCAGGCGGGCGTGCACTCCGGT > minE/30750‑30864 | ggACCACTTCCTTGATGACGCAGTAGAAGTTGACGTGGATGCCATCTGCGACGGCGAAGTGGTGCTGa < 1:3049908/68‑1 (MQ=255)ggACCACTTCCTTGATGACGCAGTAGAAGTTGACGTGGATGCCATCTGCGACGGCGAAGTGGTGCTGa < 1:1999567/68‑1 (MQ=255)ggACCACTTCCTTGATGACGCAGTAGAAGTTGACGTGGATGCCATCTGCGACGGCGAAGTGGTGCTGa < 1:2282211/68‑1 (MQ=255)ggACCACTTCCTTGATGACGCAGTAGAAGTTGACGTGGATGCCATCTGCGACGGCGAAGTGGTGCTGa < 1:2343199/68‑1 (MQ=255)ggACCACTTCCTTGATGACGCAGTAGAAGTTGACGTGGATGCCATCTGCGACGGCGAAGTGGTGCTGa < 1:2466997/68‑1 (MQ=255)ggACCACTTCCTTGATGACGCAGTAGAAGTTGACGTGGATGCCATCTGCGACGGCGAAGTGGTGCTGa < 1:2572474/68‑1 (MQ=255)ggACCACTTCCTTGATGACGCAGTAGAAGTTGACGTGGATGCCATCTGCGACGGCGAAGTGGTGCTGa < 1:2743522/68‑1 (MQ=255)ggACCACTTCCTTGATGACGCAGTAGAAGTTGACGTGGATGCCATCTGCGACGGCGAAGTGGTGCTGa < 1:2874563/68‑1 (MQ=255)ggACCACTTCCTTGATGACGCAGTAGAAGTTGACGTGGATGCCATCTGCGACGGCGAAGTGGTGCTGa < 1:2898232/68‑1 (MQ=255)ggACCACTTCCTTGATGACGCAGTAGAAGTTGACGTGGATGCCATCTGCGACGGCGAAGTGGTGCTGa < 1:3006207/68‑1 (MQ=255)ggACCACTTCCTTGATGACGCAGTAGAAGTTGACGTGGATGCCATCTGCGACGGCGAAGTGGTGCTGa < 1:1046790/68‑1 (MQ=255)ggACCACTTCCTTGATGACGCAGTAGAAGTTGACGTGGATGCCATCTGCGACGGCGAAGTGGTGCTGa < 1:3050845/68‑1 (MQ=255)ggACCACTTCCTTGATGACGCAGTAGAAGTTGACGTGGATGCCATCTGCGACGGCGAAGTGGTGCTGa < 1:3067698/68‑1 (MQ=255)ggACCACTTCCTTGATGACGCAGTAGAAGTTGACGTGGATGCCATCTGCGACGGCGAAGTGGTGCTGa < 1:3107562/68‑1 (MQ=255)ggACCACTTCCTTGATGACGCAGTAGAAGTTGACGTGGATGCCATCTGCGACGGCGAAGTGGTGCTGa < 1:3204779/68‑1 (MQ=255)ggACCACTTCCTTGATGACGCAGTAGAAGTTGACGTGGATGCCATCTGCGACGGCGAAGTGGTGCTGa < 1:322576/68‑1 (MQ=255)ggACCACTTCCTTGATGACGCAGTAGAAGTTGACGTGGATGCCATCTGCGACGGCGAAGTGGTGCTGa < 1:511701/68‑1 (MQ=255)ggACCACTTCCTTGATGACGCAGTAGAAGTTGACGTGGATGCCATCTGCGACGGCGAAGTGGTGCTGa < 1:722286/68‑1 (MQ=255)ggACCACTTCCTTGATGACGCAGTAGAAGTTGACGTGGATGCCATCTGCGACGGCGAAGTGGTGCTGa < 1:789513/68‑1 (MQ=255)ggACCACTTCCTTGATGACGCAGTAGAAGTTGACGTGGATGCCATCTGCGACGGCGAAGTGGTGCTGa < 1:963083/68‑1 (MQ=255)ggACCACTTCCTTGATGACGCAGTAGAAGTTGACGTGGATGCCATCTGCGACGGCGAAGTGGTGCTGa < 1:1031731/68‑1 (MQ=255)ggACCACTTCCTTGATGACGCAGTAGAAGTTGACGTGGATGCCATCTGCGACGGCGAAGTGGTGCTGa < 1:1334288/68‑1 (MQ=255)ggACCACTTCCTTGATGACGCAGTAGAAGTTGACGTGGATGCCATCTGCGACGGCGAAGTGGTGCTGa < 1:1262517/68‑1 (MQ=255)ggACCACTTCCTTGATGACGCAGTAGAAGTTGACGTGGATGCCATCTGCGACGGCGAAGTGGTGCTGa < 1:1810520/68‑1 (MQ=255)ggACCACTTCCTTGATGACGCAGTAGAAGTTGACGTGGATGCCATCTGCGACGGCGAAGTGGTGCTGa < 1:1807038/68‑1 (MQ=255)ggACCACTTCCTTGATGACGCAGTAGAAGTTGACGTGGATGCCATCTGCGACGGCGAAGTGGTGCTGa < 1:1680650/68‑1 (MQ=255)ggACCACTTCCTTGATGACGCAGTAGAAGTTGACGTGGATGCCATCTGCGACGGCGAAGTGGTGCTGa < 1:1721426/68‑1 (MQ=255)ggACCACTTCCTTGATGACGCAGTAGAAGTTGACGTGGATGCCATCTGCGACGGCGAAGTGGTGCTGa < 1:1210991/68‑1 (MQ=255)ggACCACTTCCTTGATGACGCAGTAGAAGTTGACGTGGATGCCATCTGCGACGGCGAAGTGGTGCTGa < 1:1113965/68‑1 (MQ=255)ggACCACTTCCTTGATGACGCAGTAGAAGTTGACGTGGATGCCATCTGCGACGGCGAAGTGGTCCTGa < 1:2041289/68‑1 (MQ=255) tCCTCGATGACGCGGTAGAAGTTGACGTGGATGCCATCTGCGACGGCGAAATGGTGCTGATTggcggc > 1:626419/1‑68 (MQ=255) tCCTCGATGACGCGGTAGAAGTTGACGTGGATGCCATCTGCGACGGCGAAATGGTGCTGATTggcggc > 1:1372188/1‑68 (MQ=255) tCCTCGATGACGCGGTAGAAGTTGACGTGGATGCCATCTGCGACGGCGAAATGGTGCTGATTggcggc > 1:2900664/1‑68 (MQ=255) tCCTCGATGACGCGGTAGAAGTTGACGTGGATGCCATCTGCGACGGCGAAATGGTGCTGATTggcggc > 1:1208107/1‑68 (MQ=255) agTAGAAGTTGACGTGGATGCCATCTGCGACTGCGAAGTGGTGCTGATTGGCGGCATCATGGAGCa < 1:734947/65‑1 (MQ=255) agTAGAAGTTGACGTGGATGCCATCTGCGACGGCGAAGTGGTGCTGATTGGCGGCATCATGGAGCa < 1:423473/65‑1 (MQ=255) agTAGAAGTTGACGTGGATGCCATCTGCGACGGCGAAGTGGTGCTGATTGGCGGCATCATGGAGCa < 1:1624707/65‑1 (MQ=255) agTAGAAGTTGACGTGGATGCCATCTGCGACGGCGAAGTGGTGCTGATTGGCGGCATCATGGAGCa < 1:690035/65‑1 (MQ=255) agTAGAAGTTGACGTGGATGCCATCTGCGACGGCGAAGTGGTGCTGATTGGCGGCATCATGGAGCa < 1:1071675/65‑1 (MQ=255) agTAGAAGTTGACGTGGATGCCATCTGCGACGGCGAAGTGGTGCTGATTGGCGGCATCATGGAGCa < 1:926922/65‑1 (MQ=255) agTAGAAGTTGACGTGGATGCCATCTGCGACGGCGAAGTGGTGCTGATTGGCGGCATCATGGAGCa < 1:3063971/65‑1 (MQ=255) agTAGAAGTTGACGTGGATGCCATCTGCGACGGCGAAGTGGTGCTGATTGGCGGCATCATGGAGCa < 1:96145/65‑1 (MQ=255) agTAGAAGTTGACGTGGATGCCATCTGCGACGGCGAAGTGGTGCTGATTGGCGGCATCATGGAGCa < 1:1559678/65‑1 (MQ=255) agTAGAAGTTGACGTGGATGCCATCTGCGACGGCGAAGTGGTGCTGATTGGCGGCATCATGGAGCa < 1:2984592/65‑1 (MQ=255) agTAGAAGTTGACGTGGATGCCATCTGCGACGGCGAAGTGGTGCTGATTGGCGGCATCATGGAGCa < 1:2964834/65‑1 (MQ=255) agTAGAAGTTGACGTGGATGCCATCTGCGACGGCGAAGTGGTGCTGATTGGCGGCATCATGGAGCa < 1:2787413/65‑1 (MQ=255) agTAGAAGTTGACGTGGATGCCATCTGCGACGGCGAAGTGGTGCTGATTGGCGGCATCATGGAGCa < 1:2024137/65‑1 (MQ=255) agTAGAAGTTGACGTGGATGCCATCTGCGACGGCGAAGTGGTGCTGATTGGCGGCATCATGGAGCa < 1:207301/65‑1 (MQ=255) agTAGAAGTTGACGTGGATGCCATCTGCGACGGCGAAGTGGTGCTGATTGGCGGCATCATGGAGCa < 1:2601727/65‑1 (MQ=255) agTAGAAGTTGACGTGGATGCCATCTGCGACGGCGAAGTGGTGCTGATTGGCGGCATCATGGAGCa < 1:2507647/65‑1 (MQ=255) agTAGAAGTTGACGTGGATGCCATCTGCGACGGCGAAGTGGTGCTGATTGGCGGCATCATGGAGCa < 1:238267/65‑1 (MQ=255) agTAGAAGTTGACGTGGATGCCATCTGCGACGGCGAAGTGGTGCTGATTGGCGGCATCATGGAGCa < 1:2274812/65‑1 (MQ=255) agTAGAAGTTGACGTGGATGCCATCTGCGACGGCGAAGTGGTGCTAATTGGCGGCATCATGGAGCa < 1:301714/65‑1 (MQ=255) gAAGTTGACGTGGATGCCATCTGCGACGGCGAAATGGTGCt < 1:1890917/41‑1 (MQ=255) gAAGTTGACGTGGATGCCATCTGCGACGGCGAAATGGTGCt < 1:1642676/41‑1 (MQ=255) gAAGTTGACGTGGATGCCATCTGCGACGGCGAAATGGTGCt < 1:1083067/41‑1 (MQ=255) gAAGTTGACGTGGATGCCATCTGCGACGGCGAAATGGTGCt < 1:803404/41‑1 (MQ=255) tGCGACGGCGAAATGGTGCTGATTGGCGGCATCATGGAGCATATTGAGCAGGCGGGCGTGCACTCCGGt > 1:1720913/1‑69 (MQ=255) tGCGACGGCGAAATGGTGCTGATTGGCGGCATCATGGAGCATATTGAGCAGGCGGGCGTGCACTCCGGt > 1:2238272/1‑69 (MQ=255) tGCGACGGCGAAATGGTGCTGATTGGCGGCATCATGGAGCATATTGAGCAGGCGGGCGTGCACTCCGGt > 1:3368564/1‑69 (MQ=255) tGCGACGGCGAAATGGTGCTGATTGGCGGCATCATGGAGCATATTGAGCAGGCGGGCGTGCACTCCGGt > 1:1699877/1‑69 (MQ=255) tGCGACGGCGAAATGGTGCTGATTGGCGGCATCATGGAGCATATTGAGCAGGCGGGCGTGCACTCCGGt > 1:1893669/1‑69 (MQ=255) | GGACCACTTCCTCGATGACGCGGTAGAAGTTGACGTGGATGCCATCTGCGACGGCGAAATGGTGCTGATTGGCGGCATCATGGAGCATATTGAGCAGGCGGGCGTGCACTCCGGT > minE/30750‑30864 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |