| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 266,955 | G→A | 63.0% | G564G (GGC→GGT) | dxs ← | 1‑deoxyxylulose‑5‑phosphate synthase, thiamine‑requiring, FAD‑requiring |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 266,955 | 0 | G | A | 63.0% | 55.7 / 63.3 | 100 | G564G (GGC→GGT) | dxs | 1‑deoxyxylulose‑5‑phosphate synthase, thiamine‑requiring, FAD‑requiring |
| Reads supporting (aligned to +/- strand): ref base G (30/7); new base A (34/29); total (64/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.16e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.19e-01 | |||||||||||
Only 100 of 114 total aligned reads displayed.
GTTCAGCACGGGTACTGGTTTACGATGGGCCATCAGCACTTCGTTCACGCCGCTGCCTGCGCCGCCCATAATGGCGTTTTCTTCTACGGTGACCAGCGCTTCATGGCTGGCGGCCATTTCCAGAATT > minE/266895‑267021 | gTTCATCACGGGTACTGGTTTACGATGGGCCATCAGCACTTCGTTCACGCCGCTGCCTGCGCCGCCCAt < 1:3362027/69‑1 (MQ=255)gTTCAGCACGGGTACTGGTTTACGATGGGCCATCAGCACTTCGTTCACGCCGCTGCCTGCGCCGCCCAt < 1:2340898/69‑1 (MQ=255)gTTCAGCACGGGTACTGGTTTACGATGGGCCATCAGCACTTCGTTCACGCCGCTGCCTGCGCCGCCCAt < 1:2497282/69‑1 (MQ=255)gTTCAGCACGGGTACTGGTTTACGATGGGCCATCAGCACTTCGTTCACGCCGCTGCCTGCGCCGCCCAt < 1:771623/69‑1 (MQ=255)gTTCAGCACGGGTACTGGTTTACGATGGGCCATCAGCACTTCGTTCACGCCGCTGCCTGCGCCGCCCAt < 1:1389585/69‑1 (MQ=255) cAGAACGGGTACTGGTTTACGATGGGCCATCAGCACTTCGTTCACGCCGCTGCCTGCGCCGCCCATAAt > 1:2025259/1‑69 (MQ=255) cTGGTTTACGATGGGCCATCAGCACTTCGTTCACGCCGCTGCCTGCgccgcc < 1:1000379/52‑1 (MQ=255) cTGGTTTACGATGGGCCATCAGCACTTCGTTCACGCCGCTGCCTGCgccgcc < 1:2052980/52‑1 (MQ=255) cTGGTTTACGATGGGCCATCAGCACTTCGTTCACGCCGCTGCCTGCgccgcc < 1:2673952/52‑1 (MQ=255) cTGGTTTACGATGGGCCATCAGCACTTCGTTCACGCCGCTGCCTGCgccgcc < 1:1780028/52‑1 (MQ=255) cTGGTTTACGATGGGCCATCAGCACTTCGTTCACGCCGCTGCCTGCgccgcc < 1:3226880/52‑1 (MQ=255) cTGGTTTACGATGGGCCATCAGCACTTCGTTCACGCCGCTGCCTGCgccgcc < 1:370650/52‑1 (MQ=255) tGGTTTACGATGGGCCATCAGCACTTCGTTCACGCCGCTGCCTGCgccgcc < 1:1113280/51‑1 (MQ=255) gTTTACGATGGGCCATCAGCACTTCGTTCACGCCGCTGCCTGCACCACCCATAATGGCGTTTTCTTCTa > 1:1468687/1‑69 (MQ=255) gTTTACGATGGGCCATCAGCACTTCGTTCACGCCGCTGCCTGCACCACCCATAATGGCGTTTTCTTCTa > 1:963031/1‑69 (MQ=255) gTTTACGATGGGCCATCAGCACTTCGTTCACGCCGCTGCCTGCACCACCCATAATGGCGTTTTCTTCTa > 1:2203569/1‑69 (MQ=255) gTTTACGATGGGCCATCAGCACTTCGTTCACGCCGCTGCCTGCACCACCCATAATGGCGTTTTCTTCTa > 1:962911/1‑69 (MQ=255) gTTTACGATGGGCCATCAGCACTTCGTTCACGCCGCTGCCTGCACCACCCATAATGGCGTTTTCTTCTa > 1:2584603/1‑69 (MQ=255) gTTTACGATGGGCCATCAGCACTTCGTTCACGCCGCTGCCTGCACCACCCATAATGGCGTTTTCTTCTa > 1:2808743/1‑69 (MQ=255) gTTTACGATGGGCCATCAGCACTTCGTTCACGCCGCTGCCTGCACCACCCATAATGGCGTTTTCTTCTa > 1:2862946/1‑69 (MQ=255) gTTTACGATGGGCCATCAGCACTTCGTTCACGCCGCTGCCTGCACCACCCATAATGGCGTTTTCTTCTa > 1:2978183/1‑69 (MQ=255) gTTTACGATGGGCCATCAGCACTTCGTTCACGCCGCTGCCTGCACCACCCATAATGGCGTTTTCTTCTa > 1:3015846/1‑69 (MQ=255) gTTTACGATGGGCCATCAGCACTTCGTTCACGCCGCTGCCTGCACCACCCATAATGGCGTTTTCTTCTa > 1:1488895/1‑69 (MQ=255) gTTTACGATGGGCCATCAGCACTTCGTTCACGCCGCTGCCTGCACCACCCATAATGGCGTTTTCTTCTa > 1:2983731/1‑69 (MQ=255) ttACGATGGGGCATCAGCACTTCGTTCACGCCGCTGCCTGCACCACCCAt < 1:2063880/50‑1 (MQ=38) ttACGATGGGCCATCAGCACTTCGTTCACGCCGCTGCCTGCACCACCCAt < 1:256479/50‑1 (MQ=39) ttACGATGGGCCATCAGCACTTCGTTCACGCCGCTGCCTGCACCACCCAt < 1:1058740/50‑1 (MQ=39) ttACGATGGGCCATCAGCACTTCGTTCACGCCGCTGCCTGCACCACCCAt < 1:482988/50‑1 (MQ=39) ttACGATGGGCCATCAGCACTTCGTTCACGCCGCTGCCTGCACCACCCAt < 1:2292776/50‑1 (MQ=39) ttACGATGGGCCATCAGCACTTCGTTCACGCCGCTGCCTGCACCACCCAt < 1:519729/50‑1 (MQ=39) ttACGATGGGCCATCAGCACTTCGTTCACGCCGCTGCCTGCACCACCCAt < 1:2405674/50‑1 (MQ=39) ttACGATGGGCCATCAGCACTTCGTTCACGCCGCTGCCTGCACCACCCAt < 1:318651/50‑1 (MQ=39) ttACGATGGGCCATCAGCACTTCGTTCACGCCGCTGCCTGCACCACCCAt < 1:2566078/50‑1 (MQ=39) ttACGATGGGCCATCAGCACTTCGTTCACGCCGCTGCCTGCACCACCCAt < 1:3139899/50‑1 (MQ=39) ttACGATGGGCCATCAGCACTTCGTTCACGCCGCTGCCTGCACCACCCAt < 1:560682/50‑1 (MQ=39) ttACGATGGGCCATCAGCACTTCGTTCACGCCGCTGCCTGCACCACCCAt < 1:53032/50‑1 (MQ=39) ttACGATGGGCCATCAGCACTTCGTTCACGCCGCTGCCTGCACCACCCAt < 1:3001002/50‑1 (MQ=39) ttACGATGGGCCATCAGCACTTCGTTCACGCCGCTGCCTGCACCACCCAt < 1:2865188/50‑1 (MQ=39) ttACGATGGGCCATCAGCACTTCGTTCACGCCGCTGCCTGCACCACCCAt < 1:2036503/50‑1 (MQ=39) ttACGATGGGCCATCAGCACTTCGTTCACGCCGCTGCCTGCACCACCCAt < 1:1480751/50‑1 (MQ=39) ttACGATGGGCCATCAGCACTTCGTTCACGCCGCTGCCTGCACCACCCAt < 1:1503324/50‑1 (MQ=39) ttACGATGGGCCATCAGCACTTCGTTCACGCCGCTGCCTGCACCACCCAt < 1:1158514/50‑1 (MQ=39) ttACGATGGGCCATCAGCACTTCGTTCACGCCGCTGCCTGCACCACCCAt < 1:1523232/50‑1 (MQ=39) ttACGATGGGCCATCAGCACTTCGTTCACGCCGCTGCCTGCACCACCCAt < 1:1690073/50‑1 (MQ=39) ttACGATGGGCCATCAGCACTTCGTTCACGCCGCTGCCTGCACCACCCAt < 1:1693165/50‑1 (MQ=39) ttACGATGGGCCATCAGCACTTCGTTCACGCCGCTGCCTGCACCACCCAt < 1:1747556/50‑1 (MQ=39) ttACGATGGGCCATCAGCACTTCGTTCACGCCGCTGCCTGCACCACCCAt < 1:1275837/50‑1 (MQ=39) ttACGATGGGCCATCAGCACTTCGTTCACGCCGCTGCCTGCACCACCCAt < 1:1391416/50‑1 (MQ=39) ttACGATGGGCCATCAGCACTTCGTACACGCCGCTGCCTGCACCACCCAt < 1:3201645/50‑1 (MQ=255) gATGGGCCATCAGCACTTCGTTCACGCCGCTGCCTGCgccgcc < 1:85874/43‑1 (MQ=255) tGGGCCATCAGCACTTCGTTCACGCCGCTGCCTGCACCACCCAt < 1:2145781/44‑1 (MQ=38) cATCAGCACTTCGTTCACGCCGCTGCCTGCACCACCCAt < 1:108849/39‑1 (MQ=37) aTCAGCACTTCGTTCACGCCGCTGCCTGCACCACCTATAATGGCGTTTTCTTCTACGGTAACCAgcgc > 1:1597235/1‑68 (MQ=255) aTCAGCACTTCGTTCACGCCGCTGCCTGCACCACCCATAATGGCTTTTTCTTCTACGGTAACCAgcgc > 1:3353804/1‑68 (MQ=255) aTCAGCACTTCGTTCACGCCGCTGCCTGCACCACCCATAATGGCGTTTTCTTCTAc > 1:49406/1‑56 (MQ=255) aTCAGCACTTCGTTCACGCCGCTGCCTGCACCACCCATAATGGCGTTTTCTTCTACGGTAACCAgcgc > 1:1863232/1‑68 (MQ=255) aTCAGCACTTCGTTCACGCCGCTGCCTGCACCACCCATAATGGCGTTTTCTTCTACGGTAACCAgcgc > 1:1991146/1‑68 (MQ=255) aTCAGCACTTCGTTCACGCCGCTGCCTGCACCACCCATAATGGCGTTTTCTTCTACGGTAACCAgcgc > 1:3420541/1‑68 (MQ=255) aTCAGCACTTCGTTCACGCCGCTGCCTGCACCACCCATAATGGCGTTTTCTTCTACGGTAACCAgcgc > 1:3232808/1‑68 (MQ=255) aTCAGCACTTCGTTCACGCCGCTGCCTGCACCACCCATAATGGCGTTTTCTTCTACGGTAACCAgcgc > 1:2357602/1‑68 (MQ=255) aTCAGCACTTCGTTCACGCCGCTGCCTGCACCACCCATAATGGCGTTTTCTTCTACGGTAACCAgcgc > 1:1978725/1‑68 (MQ=255) aTCAGCACTTCGTTCACGCCGCTGCCTGCACCACCCATAATGGCGTTTTCTTCTACGGTAACCAgcgc > 1:1533365/1‑68 (MQ=255) aTCAGCACTTCGTTCACGCCGCTGCCTGCACCACCCATAATGGCGTTTTCTTCTACGGTAACCAgcgc > 1:3072290/1‑68 (MQ=255) aTCAGCACTTCGTTCACGCCGCTGCCTGCACCACCCATAATGGCGTTTTCTTCTACGGTAACCAgcgc > 1:1549274/1‑68 (MQ=255) aTCAGCACTTCGTTCACGCCGCTGCCTGCACCACCCATAATGGCGTTTTCTTCTACGGTAACCAgcgc > 1:395687/1‑68 (MQ=255) aTCAGCACTTCGTTCACGCCGCTGCCTGCACCACCCATAATGGCGTTTTCTTCTACGGTAACCAgcgc > 1:1620899/1‑68 (MQ=255) aTCAGCACTTCGTTCACGCCGCTGCCTGCACCACCCATAATGGCGTTTTCTTCTACGGTAACCAgcgc > 1:677025/1‑68 (MQ=255) aTCAGCACTTCGTTCACGCCGCTGCCTGCACCACCCATAATGGCGTTTTCTTCTACGGTAACCAgcgc > 1:2839739/1‑68 (MQ=255) aTCAGCACTTCGTTCACGCCGCTGCCTGCACCACCCATAATGGCGTTTTCTTCTACGGTAACCAgcgc > 1:1976556/1‑68 (MQ=255) aTCAGCACTTCGTTCACGCCGCTGCCTGCACCACCCATAATGGCGTTTTCTTCTACGGTAACCAgcgc > 1:2749034/1‑68 (MQ=255) aTCAGCACTTCGTTCACGCCGCTGCCTGCACCACCCATAATGGCGTTTTCTTCTACGGTAACCAgcgc > 1:2614473/1‑68 (MQ=255) aTCAGCACTTCGTTCACGCCGCTGCCTGCACCACCCATAATGGCGTTTTCTTCTACGGTAACCAgcgc > 1:2587611/1‑68 (MQ=255) aTCAGCACTTCGTTCACGCCGCTGCCTGCACCACCCATAATGGCGTTTTCTTCTACGGTAACCAgcgc > 1:490496/1‑68 (MQ=255) aTCAGCACTTCGTTCACGCAGCTGCCTGCACCACCCATAATGGCGTTTTCTTCTACGGTAACCAgcgc > 1:3094062/1‑68 (MQ=255) aGCACTTCGTTCACGCCGCTGCCTGCGCCGCCCATAATGGCGTTttcttc > 1:755114/1‑50 (MQ=255) aGCACTTCGTTCACGCCGCTGCCTGCGCCGCCCATAATGGCGTTttcttc > 1:1203341/1‑50 (MQ=255) aGCACTTCGTTCACGCCGCTGCCTGCGCCGCCCATAATGGCGTTttcttc > 1:380245/1‑50 (MQ=255) aGCACTTCGTTCACGCCGCTGCCTGCGCCGCCCATAATGGCGTTttcttc > 1:447640/1‑50 (MQ=255) aGCACTTCGTTCACGCCGCTGCCTGCGCCGCCCATAATGGCGTTttcttc > 1:786500/1‑50 (MQ=255) aGCACTTCGTTCACGCCGCTGCCTGCGCCGCCCATAATGGCGTTttcttc > 1:2132546/1‑50 (MQ=255) aGCACTTCGTTCACGCCGCTGCCTGCGCCGCCCATAATGGCGTTttcttc > 1:3104726/1‑50 (MQ=255) aGCACTTCGTTCACGCCGCTGCCTGCGCCGCCCATAATGGCGTTttcttc > 1:2797895/1‑50 (MQ=255) aGCACTTCGTTCACGCCGCTGCCTGCGCCGCCCATAATGGCGTTttcttc > 1:2137466/1‑50 (MQ=255) aGCACTTCGTTCACGCCGCTGCCTGCGCCGCCCATAATGGCGTTTTCTTCTACGGTGACCAGCGCTTCa > 1:340864/1‑69 (MQ=255) aGCACTTCGTTCACGCCGCTGCCTGCGCCGCCCATAATGGCGTTTTCTTCTACGGTGACCAGCGCTTCa > 1:1405856/1‑69 (MQ=255) aGCACTTCGTTCACGCCGCTGCCTGCGCCGCCCATAATGGCGTTTTCTTCTACGGTGACCAGCGCTTCa > 1:1915066/1‑69 (MQ=255) aGCACTTCGTTCACGCCGCTGCCTGCGCCGCCCATAATGGCGTTTTCTTCTACGGTGACCAGCGCTTCa > 1:1999947/1‑69 (MQ=255) aGCACTTCGTTCACGCCGCTGCCTGCGCCGCCCATAATGGCGTTTTCTTCTACGGTGACCAGCGCTTCa > 1:1936755/1‑69 (MQ=255) aGCACTTCGTTCACGCCGCTGCCTGCGCCGCCCATAATGGCGTTTTCTTCTACGGTGACCAGCGCTTCa > 1:2409066/1‑69 (MQ=255) tCGTTCACGCCGCTGCCTGCGCCGCCCATAATGGCGTTTTCTTCTAc > 1:2464670/1‑47 (MQ=255) tCGTTCACGCCGCTGCCTGCGCCGCCCATAATGGCGTTTTCTTCTACGGTGACCAGCGCTTCAtggctg > 1:3313790/1‑69 (MQ=255) tCGTTCACGCCGCTGCCTGCGCCGCCCATAATGGCGTTTTCTTCTACGGTGACCAGCGCTTCAtggctg > 1:1587015/1‑69 (MQ=255) tCGTTCACGCCGCTGCCTGCGCCGCCCATAATGGCGTTTTCTTCTACGGTGACCAGCGCTTCAtggct > 1:1811424/1‑68 (MQ=255) tCGTTCACGCCGCTGCCTGCGCCGCCCATAATGGCGTTTTCTTCTACGGTGACCAGCGCTTCAtggc > 1:1083126/1‑67 (MQ=255) cctgcGCCGCCCATAATGGCGTTTTCTTCTACGGTGACCAGCGCTTCATGGCTGGCGGCCATTTCCAGa > 1:248587/1‑69 (MQ=255) cctgcGCCGCCCATAATGGCGTTTTCTTCTACGGTGACCAGCGCTTCATGGCTGGCGGCCATTTCCAGa > 1:331915/1‑69 (MQ=255) cctgcGACGCCCATAATGGCGTTTTCTTCTACGGTGACCAGCGCTTCATGGCTGGCGGCCATTTCCAGa > 1:1979142/1‑69 (MQ=255) gcgcCGCCCATAATGGCGTTTTCTTCTACGGTGACCAGCGCTTCATGGCTGGCGGCCATTTCCAGAAtt < 1:2295504/69‑1 (MQ=255) gcgcCGCCCATAATGGCGTTTTCTTCTACGGTGACCAGCGCTTCATGGCTGGCGGCCATTTCCAGAAtt < 1:138795/69‑1 (MQ=255) cgccgcCCATAATGGCGTTTTCTTCTACGGTGACCAGCGCTTCATGGCTGGCGGCCATTTCCAGAAtt < 1:2579598/68‑1 (MQ=255) | GTTCAGCACGGGTACTGGTTTACGATGGGCCATCAGCACTTCGTTCACGCCGCTGCCTGCGCCGCCCATAATGGCGTTTTCTTCTACGGTGACCAGCGCTTCATGGCTGGCGGCCATTTCCAGAATT > minE/266895‑267021 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |