| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 267,465 | A→G | 100% | T394T (ACT→ACC) | dxs ← | 1‑deoxyxylulose‑5‑phosphate synthase, thiamine‑requiring, FAD‑requiring |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 267,465 | 0 | A | G | 84.4% | 96.4 / 6.9 | 57 | T394T (ACT→ACC) | dxs | 1‑deoxyxylulose‑5‑phosphate synthase, thiamine‑requiring, FAD‑requiring |
| Reads supporting (aligned to +/- strand): ref base A (0/9); new base G (40/8); total (40/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.70e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.24e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATGGGTTTGTACCCACCAATCGCCAGACCCGCAGCA > minE/267397‑267519 | gCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGaaa < 1:437717/69‑1 (MQ=255)gCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGaaa < 1:3174573/69‑1 (MQ=255)gCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGaaa < 1:2877522/69‑1 (MQ=255)gCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGaaa < 1:1756273/69‑1 (MQ=255) cGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGaaa < 1:1896382/68‑1 (MQ=255) gACCGGAAGCTTTTGAATCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTa < 1:1091924/69‑1 (MQ=255) gACCGGAAGCTTTTGAATCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTa < 1:1496290/69‑1 (MQ=255) gACCGGAAGCTTTTGAATCGCCACGTCATGCAGCACCTGATCATAGGCGCCTTGCAGGAAAGTGGAGTa < 1:1984572/69‑1 (MQ=255) aaTCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATgg < 1:1099099/68‑1 (MQ=255) aaTCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATgg < 1:470047/68‑1 (MQ=255) aaTCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATgg < 1:46182/68‑1 (MQ=255) aaTCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATgg < 1:1969040/68‑1 (MQ=255) tCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAGGTGGAGt > 1:995895/1‑51 (MQ=255) tCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAAt > 1:1607685/1‑64 (MQ=255) tCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAATg > 1:1689191/1‑65 (MQ=255) tCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAATg > 1:3306147/1‑65 (MQ=255) tCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAATg > 1:3282900/1‑65 (MQ=255) tCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAATg > 1:328034/1‑65 (MQ=255) tCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAATg > 1:1461589/1‑65 (MQ=255) tCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAATg > 1:313625/1‑65 (MQ=255) tCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAATg > 1:2832444/1‑65 (MQ=255) tCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAATg > 1:280863/1‑65 (MQ=255) tCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAATg > 1:263637/1‑65 (MQ=255) tCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAATg > 1:2041280/1‑65 (MQ=255) tCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAATg > 1:1684524/1‑65 (MQ=255) gCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCTCAACa > 1:1565067/1‑60 (MQ=255) gCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCTACAATGGGtt > 1:2306560/1‑67 (MQ=255) gCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAATGGGtt > 1:784107/1‑67 (MQ=255) gCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAATGGGtt > 1:2293556/1‑67 (MQ=255) gCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAATGGGtt > 1:92928/1‑67 (MQ=255) gCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAATGGGtt > 1:506181/1‑67 (MQ=255) gCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAATGGGtt > 1:1650724/1‑67 (MQ=255) gCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAATGGGtt > 1:2669036/1‑67 (MQ=255) gCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAATGGGtt > 1:1427720/1‑67 (MQ=255) gCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAATGGGtt > 1:2825784/1‑67 (MQ=255) gCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAATGGGtt > 1:1874212/1‑67 (MQ=255) gCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAATGGGtt > 1:3190060/1‑67 (MQ=255) gCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAATGGGtt > 1:3419236/1‑67 (MQ=255) gCCACGTCATGCAGCACCTGATAATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAATGGGtt > 1:869655/1‑67 (MQ=255) caCCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATGGGTTTGTACCCACCAATCGc < 1:823779/69‑1 (MQ=255) caCCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATGGGTTTGTACCCACCAATCGc < 1:20259/69‑1 (MQ=255) aCCTGATCATAGGCGCGTTTCAGGAAGGTGGAGTAGATCGCAACAATGGGTTTGTAGCCTCCAATc > 1:1432310/1‑66 (MQ=255) aCCTGATCATAGGCGCGTTGCAGGTAGGTGGAGTa > 1:337641/1‑35 (MQ=37) aCCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAATGGGTTTGTAGcctcc > 1:396958/1‑62 (MQ=255) aCCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAATGGGTTTGTAGCCTCCAATc > 1:432012/1‑66 (MQ=255) aCCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAATGGGTTTGTAGCCTCCAATc > 1:1897281/1‑66 (MQ=255) aCCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAATGGGTTTGTAGCCTCCAATc > 1:1270586/1‑66 (MQ=255) aCCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAATGGGTTTGTAGCCTCCAATc > 1:717394/1‑66 (MQ=255) aCCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAATGGGTTTGTAGCCTCCAATc > 1:1875378/1‑66 (MQ=255) aCCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAATGGGTTTGTAGCCTCCAATc > 1:1475585/1‑66 (MQ=255) aCCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAATGGGTTTGTAGCCTCCAATc > 1:193886/1‑66 (MQ=255) aCCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAATGGGTTTGTAGCCTCCAATc > 1:2337692/1‑66 (MQ=255) aCCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAATGGGTTTGTAGCCTCCAATc > 1:2208084/1‑66 (MQ=255) aCCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCCCAACAATGGGTTTGTAGCCTCCAATc > 1:2351589/1‑66 (MQ=255) cgcgTTGCAGGAAGGTGGAGTAGATCGCAACAATGGGTTTGTAGCCTCCAATCGCCAGACCCgcagca < 1:3206777/68‑1 (MQ=255) cgcgTTGCAGGAAGGTGGAGTAGATCGCAACAATGGGTTTGTAGCCTCCAATCGCCAGACCCgcagca < 1:90811/68‑1 (MQ=255) cgcgTTGCAGGAAGGTGGAGTAGATCGCAACAATGGGTTTGTAGCCTCCAATCGCCAGACCCgcagca < 1:1226057/68‑1 (MQ=255) cgcgTTGCAGGAAGGTGGAGTAGATCGCAACAATGGGTTTGTAGCCTCCAATCGCCAGACCCgcagca < 1:3110022/68‑1 (MQ=255) cgcgTTGCAGGAAGGTGGAGTAGATCGCAACAATGGGTTTGTAGCCTCCAATCGCCAGACCCgcagca < 1:157190/68‑1 (MQ=255) cgcgTTGCAGGAAGGTGGAGTAGATCGCAACAATGGGTTTGTAGCCTCCAATCGCCAGACCCgcagca < 1:2321842/68‑1 (MQ=255) cgcgTTGCAGGAAGGTGGAGTAGATCGCAACAATGGGTTTGTAGCCTCCAATCGCCAGACCCgcagca < 1:200264/68‑1 (MQ=255) gcgTTGCAGGAAGGTGGAGTAGATCGCAACAATCGGTTTGTAGCCTCCAATCGCCAGACCCgcagca < 1:2828582/67‑1 (MQ=255) | GCGAACAGGACCGGAAGCTTTTGAATCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATGGGTTTGTACCCACCAATCGCCAGACCCGCAGCA > minE/267397‑267519 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |