Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 267,474 A→G 100% I391I (ATT→ATC)  dxs ← 1‑deoxyxylulose‑5‑phosphate synthase, thiamine‑requiring, FAD‑requiring

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE267,4740AG88.7% 97.6 / 1.9 52I391I (ATT→ATC) dxs1‑deoxyxylulose‑5‑phosphate synthase, thiamine‑requiring, FAD‑requiring
Reads supporting (aligned to +/- strand):  ref base A (0/6);  new base G (38/8);  total (38/14)
Fisher's exact test for biased strand distribution p-value = 1.48e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AATCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATGGGTTTGTACCCACCAATCGCCAGACCCGCAGCA  >  minE/267420‑267519
                                                      |                                             
aaTCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATgg                                  <  1:46182/68‑1 (MQ=255)
aaTCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATgg                                  <  1:1099099/68‑1 (MQ=255)
aaTCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATgg                                  <  1:1969040/68‑1 (MQ=255)
aaTCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATgg                                  <  1:470047/68‑1 (MQ=255)
  tCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAAt                                    >  1:1607685/1‑64 (MQ=255)
  tCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAATg                                   >  1:1689191/1‑65 (MQ=255)
  tCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAATg                                   >  1:263637/1‑65 (MQ=255)
  tCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAATg                                   >  1:2041280/1‑65 (MQ=255)
  tCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAATg                                   >  1:280863/1‑65 (MQ=255)
  tCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAATg                                   >  1:2832444/1‑65 (MQ=255)
  tCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAATg                                   >  1:313625/1‑65 (MQ=255)
  tCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAATg                                   >  1:1684524/1‑65 (MQ=255)
  tCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAATg                                   >  1:328034/1‑65 (MQ=255)
  tCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAATg                                   >  1:1461589/1‑65 (MQ=255)
  tCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAATg                                   >  1:3282900/1‑65 (MQ=255)
  tCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAATg                                   >  1:3306147/1‑65 (MQ=255)
    gCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCTCAACa                                      >  1:1565067/1‑60 (MQ=255)
    gCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCTACAATGGGtt                               >  1:2306560/1‑67 (MQ=255)
    gCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAATGGGtt                               >  1:2293556/1‑67 (MQ=255)
    gCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAATGGGtt                               >  1:3190060/1‑67 (MQ=255)
    gCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAATGGGtt                               >  1:506181/1‑67 (MQ=255)
    gCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAATGGGtt                               >  1:2825784/1‑67 (MQ=255)
    gCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAATGGGtt                               >  1:2669036/1‑67 (MQ=255)
    gCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAATGGGtt                               >  1:3419236/1‑67 (MQ=255)
    gCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAATGGGtt                               >  1:1874212/1‑67 (MQ=255)
    gCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAATGGGtt                               >  1:92928/1‑67 (MQ=255)
    gCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAATGGGtt                               >  1:1427720/1‑67 (MQ=255)
    gCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAATGGGtt                               >  1:1650724/1‑67 (MQ=255)
    gCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAATGGGtt                               >  1:784107/1‑67 (MQ=255)
    gCCACGTCATGCAGCACCTGATAATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAATGGGtt                               >  1:869655/1‑67 (MQ=255)
                  caCCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATGGGTTTGTACCCACCAATCGc               <  1:823779/69‑1 (MQ=255)
                  caCCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATGGGTTTGTACCCACCAATCGc               <  1:20259/69‑1 (MQ=255)
                   aCCTGATCATAGGCGCGTTTCAGGAAGGTGGAGTAGATCGCAACAATGGGTTTGTAGCCTCCAATc                 >  1:1432310/1‑66 (MQ=255)
                   aCCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAATGGGTTTGTAGcctcc                     >  1:396958/1‑62 (MQ=255)
                   aCCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAATGGGTTTGTAGCCTCCAATc                 >  1:193886/1‑66 (MQ=255)
                   aCCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAATGGGTTTGTAGCCTCCAATc                 >  1:717394/1‑66 (MQ=255)
                   aCCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAATGGGTTTGTAGCCTCCAATc                 >  1:1897281/1‑66 (MQ=255)
                   aCCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAATGGGTTTGTAGCCTCCAATc                 >  1:1875378/1‑66 (MQ=255)
                   aCCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAATGGGTTTGTAGCCTCCAATc                 >  1:2208084/1‑66 (MQ=255)
                   aCCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAATGGGTTTGTAGCCTCCAATc                 >  1:1475585/1‑66 (MQ=255)
                   aCCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAATGGGTTTGTAGCCTCCAATc                 >  1:1270586/1‑66 (MQ=255)
                   aCCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAATGGGTTTGTAGCCTCCAATc                 >  1:432012/1‑66 (MQ=255)
                   aCCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAATGGGTTTGTAGCCTCCAATc                 >  1:2337692/1‑66 (MQ=255)
                   aCCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCCCAACAATGGGTTTGTAGCCTCCAATc                 >  1:2351589/1‑66 (MQ=255)
                                cgcgTTGCAGGAAGGTGGAGTAGATCGCAACAATGGGTTTGTAGCCTCCAATCGCCAGACCCgcagca  <  1:3206777/68‑1 (MQ=255)
                                cgcgTTGCAGGAAGGTGGAGTAGATCGCAACAATGGGTTTGTAGCCTCCAATCGCCAGACCCgcagca  <  1:3110022/68‑1 (MQ=255)
                                cgcgTTGCAGGAAGGTGGAGTAGATCGCAACAATGGGTTTGTAGCCTCCAATCGCCAGACCCgcagca  <  1:2321842/68‑1 (MQ=255)
                                cgcgTTGCAGGAAGGTGGAGTAGATCGCAACAATGGGTTTGTAGCCTCCAATCGCCAGACCCgcagca  <  1:200264/68‑1 (MQ=255)
                                cgcgTTGCAGGAAGGTGGAGTAGATCGCAACAATGGGTTTGTAGCCTCCAATCGCCAGACCCgcagca  <  1:157190/68‑1 (MQ=255)
                                cgcgTTGCAGGAAGGTGGAGTAGATCGCAACAATGGGTTTGTAGCCTCCAATCGCCAGACCCgcagca  <  1:90811/68‑1 (MQ=255)
                                cgcgTTGCAGGAAGGTGGAGTAGATCGCAACAATGGGTTTGTAGCCTCCAATCGCCAGACCCgcagca  <  1:1226057/68‑1 (MQ=255)
                                 gcgTTGCAGGAAGGTGGAGTAGATCGCAACAATCGGTTTGTAGCCTCCAATCGCCAGACCCgcagca  <  1:2828582/67‑1 (MQ=255)
                                                      |                                             
AATCGCCACGTCATGCAGCACCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATGGGTTTGTACCCACCAATCGCCAGACCCGCAGCA  >  minE/267420‑267519

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑