| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 267,495 | C→G | 64.2% | G384G (GGG→GGC) | dxs ← | 1‑deoxyxylulose‑5‑phosphate synthase, thiamine‑requiring, FAD‑requiring |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 267,495 | 0 | C | G | 64.2% | 45.7 / 45.0 | 57 | G384G (GGG→GGC) | dxs | 1‑deoxyxylulose‑5‑phosphate synthase, thiamine‑requiring, FAD‑requiring |
| Reads supporting (aligned to +/- strand): ref base C (5/15); new base G (12/24); total (17/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.61e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01 | |||||||||||
CACCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATGGGTTTGTACCCACCAATCGCCAGACCCGCAGCAAAGGTCACCGCGTGTTGCTCGGCAATTGCCACGTCGAAGT > minE/267438‑267559 | caCCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATGGGTTTGTACCCACCAATCGc < 1:823779/69‑1 (MQ=255)caCCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATGGGTTTGTACCCACCAATCGc < 1:20259/69‑1 (MQ=255) aCCTGATCATAGGCGCGTTTCAGGAAGGTGGAGTAGATCGCAACAATGGGTTTGTAGCCTCCAATc > 1:1432310/1‑66 (MQ=255) aCCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAATGGGTTTGTAGcctcc > 1:396958/1‑62 (MQ=255) aCCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAATGGGTTTGTAGCCTCCAATc > 1:1270586/1‑66 (MQ=255) aCCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAATGGGTTTGTAGCCTCCAATc > 1:2337692/1‑66 (MQ=255) aCCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAATGGGTTTGTAGCCTCCAATc > 1:2208084/1‑66 (MQ=255) aCCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAATGGGTTTGTAGCCTCCAATc > 1:432012/1‑66 (MQ=255) aCCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAATGGGTTTGTAGCCTCCAATc > 1:193886/1‑66 (MQ=255) aCCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAATGGGTTTGTAGCCTCCAATc > 1:1897281/1‑66 (MQ=255) aCCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAATGGGTTTGTAGCCTCCAATc > 1:1875378/1‑66 (MQ=255) aCCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAATGGGTTTGTAGCCTCCAATc > 1:717394/1‑66 (MQ=255) aCCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCGCAACAATGGGTTTGTAGCCTCCAATc > 1:1475585/1‑66 (MQ=255) aCCTGATCATAGGCGCGTTGCAGGAAGGTGGAGTAGATCCCAACAATGGGTTTGTAGCCTCCAATc > 1:2351589/1‑66 (MQ=255) cgcgTTGCAGGAAGGTGGAGTAGATCGCAACAATGGGTTTGTAGCCTCCAATCGCCAGACCCgcagca < 1:200264/68‑1 (MQ=255) cgcgTTGCAGGAAGGTGGAGTAGATCGCAACAATGGGTTTGTAGCCTCCAATCGCCAGACCCgcagca < 1:157190/68‑1 (MQ=255) cgcgTTGCAGGAAGGTGGAGTAGATCGCAACAATGGGTTTGTAGCCTCCAATCGCCAGACCCgcagca < 1:90811/68‑1 (MQ=255) cgcgTTGCAGGAAGGTGGAGTAGATCGCAACAATGGGTTTGTAGCCTCCAATCGCCAGACCCgcagca < 1:3206777/68‑1 (MQ=255) cgcgTTGCAGGAAGGTGGAGTAGATCGCAACAATGGGTTTGTAGCCTCCAATCGCCAGACCCgcagca < 1:3110022/68‑1 (MQ=255) cgcgTTGCAGGAAGGTGGAGTAGATCGCAACAATGGGTTTGTAGCCTCCAATCGCCAGACCCgcagca < 1:2321842/68‑1 (MQ=255) cgcgTTGCAGGAAGGTGGAGTAGATCGCAACAATGGGTTTGTAGCCTCCAATCGCCAGACCCgcagca < 1:1226057/68‑1 (MQ=255) gcgTTGCAGGAAGGTGGAGTAGATCGCAACAATCGGTTTGTAGCCTCCAATCGCCAGACCCgcagca < 1:2828582/67‑1 (MQ=255) gcgACAATGGGTTTGTACCCACCAATCGCCAGACCCGCAGCAAAGGTCACCGCGTGTTGCTc > 1:941351/1‑62 (MQ=255) gcgACAATGGGTTTGTACCCACCAATCGCCAGACCCGCAGCAAAGGTCACCGCGTGTTGCTCGGCAAtt > 1:336637/1‑69 (MQ=255) gcgACAATGGGTTTGTACCCACCAATCGCCAGACCCGCAGCAAAGGTCACCGCGTGTTGCTCGGCAAtt > 1:2825384/1‑69 (MQ=255) gcgACAATGGGTTTGTACCCACCAATCGCCAGACCCGCAGCAAAGGTCACCGCGTGTTGCTCGGCAAtt > 1:3047174/1‑69 (MQ=255) caaCAATGGGTTTGTAGCCTCCAATCGCCAGACCCgcagca < 1:2739202/39‑1 (MQ=37) caaCAATGGGTTTGTAGCCTCCAATCGCCAGACCCgcagca < 1:972663/39‑1 (MQ=37) caaCAATGGGTTTGTAGCCTCCAATCGCCAGACCCgcagca < 1:903998/39‑1 (MQ=37) caaCAATGGGTTTGTAGCCTCCAATCGCCAGACCCgcagca < 1:898411/39‑1 (MQ=37) caaCAATGGGTTTGTAGCCTCCAATCGCCAGACCCgcagca < 1:859296/39‑1 (MQ=37) caaCAATGGGTTTGTAGCCTCCAATCGCCAGACCCgcagca < 1:1630551/39‑1 (MQ=37) caaCAATGGGTTTGTAGCCTCCAATCGCCAGACCCgcagca < 1:551414/39‑1 (MQ=37) caaCAATGGGTTTGTAGCCTCCAATCGCCAGACCCgcagca < 1:5159/39‑1 (MQ=37) caaCAATGGGTTTGTAGCCTCCAATCGCCAGACCCgcagca < 1:487139/39‑1 (MQ=37) caaCAATGGGTTTGTAGCCTCCAATCGCCAGACCCgcagca < 1:206805/39‑1 (MQ=37) caaCAATGGGTTTGTAGCCTCCAATCGCCAGACCCgcagca < 1:267183/39‑1 (MQ=37) caaCAATGGGTTTGTAGCCTCCAATCGCCAGACCCgcagca < 1:2550390/39‑1 (MQ=37) caaCAATGGGTTTGTAGCCTCCAATCGCCAGACCCgcagca < 1:3217225/39‑1 (MQ=37) caaCAATGGGTTTGTAGCCTCCAATCGCCAGACCCgcagca < 1:3116811/39‑1 (MQ=37) caaCAATGGGTTTGTAGCCTCCAATCGCCAGACCCgcagca < 1:2213252/39‑1 (MQ=37) cAATGGGTTTGTACCCACCAATCGCCAGACCCGCAGCAAAGGTCACCGCGTGTTGCTCGGCAATTGCCa < 1:631388/69‑1 (MQ=255) cAATGGGTTTGTACCCACCAATCGCCAGACCCGCAGCAAAGGTCACCGCGTGTTGCTCGGCAATTGCCa < 1:2713938/69‑1 (MQ=255) cAATGGGTTTGTACCCACCAATCGCCAGACCCGCAGCAAAGGTCACCGCGTGTTGCTCGGCAATTGCCa < 1:1471804/69‑1 (MQ=255) cAATGGGTTTGTACCCACCAATCGCCAGACCCGCAGCAAAGGTCACCGCGTGTTGCTCGGCAATTGCCa < 1:898449/69‑1 (MQ=255) cAATGGGTTTGTACCCACCAATCGCCAGACCCGCAGCAAAGGTCACCGCGTGTTGCTCGGCAATTGCCa < 1:1563765/69‑1 (MQ=255) cAATGGGTTTGTACCCACCAATCGCCAGACCCGCAGCAAAGGTCACCGCGTGTTGCTCGGCAATTGCCa < 1:779599/69‑1 (MQ=255) cAATGGGTTTGTACCCACCAATCGCCAGACCCGCAGCAAAGGTCACCGCGTGTTGCTCGGCAATTGCCa < 1:2321110/69‑1 (MQ=255) cAATGGGTTTGTACCCACCAATCGCCAGACCCGCAGCAAAGGTCACCGCGTGTTGCTCGGCAATTGCCa < 1:503629/69‑1 (MQ=255) cAATGGGTTTGTACCCACCAATCGCCAGACCCGCAGCAAAGGTCACCGCGTGTTGCTCGGCAATTGCCa < 1:2052206/69‑1 (MQ=255) cAATGGGTTTGTACCCACCAATCGCCAGACCCGCAGCAAAGGTCACCGCGTGTTGCTCGGCAATTGCCa < 1:412341/69‑1 (MQ=255) cAATGGGTTTGTACCCACCAATCGCCAGACCCGCAGCAAAGGTCACCGCGTGTTGCTCGGCAATTGCCa < 1:378941/69‑1 (MQ=255) cAATGGGTTTGTACCCACCAATCGCCAGACCCGCAGCAAAGGTCACCGCGTGTTGCTCGGCAATTGCCa < 1:377679/69‑1 (MQ=255) cAATGGGTTTGTAACCACCAATCGCCAGACCCGCAGCAAAGGTCACCGCGTGTTGCTCGGCAATTGCCa < 1:2437525/69‑1 (MQ=255) aaTGGGTTTGTACCCACCAATCGCCAGACCCGCAGCAAAGGTCACCGCGTGTTGCTCGGCAATTGCCa < 1:3350616/68‑1 (MQ=255) tGGGTTTGTAGCCTCCAATCGCCAGACCCgcagca < 1:2291536/35‑1 (MQ=25) tGTACCCACCAATCGCCAGACCCGCAGCAAAGGTCACCGCGTGTTGCTCGGCAATTGCCACGTCGAAGt > 1:1442716/1‑69 (MQ=255) | CACCTGATCATAGGCGCGTTGCAGGAAAGTGGAGTAAATCGCGACAATGGGTTTGTACCCACCAATCGCCAGACCCGCAGCAAAGGTCACCGCGTGTTGCTCGGCAATTGCCACGTCGAAGT > minE/267438‑267559 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |