| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 268,176 | T→A | 33.7% | A157A (GCA→GCT) | dxs ← | 1‑deoxyxylulose‑5‑phosphate synthase, thiamine‑requiring, FAD‑requiring |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 268,176 | 0 | T | A | 33.7% | 86.3 / 68.7 | 89 | A157A (GCA→GCT) | dxs | 1‑deoxyxylulose‑5‑phosphate synthase, thiamine‑requiring, FAD‑requiring |
| Reads supporting (aligned to +/- strand): ref base T (31/28); new base A (13/17); total (44/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.03e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01 | |||||||||||
GAATCACCAGCATATCAGGACGGATATCGCCCGCGTGATTCATCGCTTCAAACGCCATGCCTGCGGTAATCGCGCCATCGCCAATGACACAGACGGTGCGGCGATTTTTGCCTTCTTTTTCGGCAGCA > minE/268115‑268242 | gaATCACCAGCATATCAGGACGGATATCGCCCGCGTGATTCATCGCTTCAAACGCCATGCCTGCGGTa < 1:1065007/68‑1 (MQ=255)gaATCACCAGCATATCAGGACGGATATCGCCCGCGTGATTCATCGCTTCAAACGCCATGCCTGCGGTa < 1:1007956/68‑1 (MQ=255)gaATCACCAGCATATCAGGACGGATATCGCCCGCGTGATTCATCGCTTCAAACGCCATGCCTGCGGTa < 1:2387181/68‑1 (MQ=255)gaATCACCAGCATATCAGGACGGATATCGCCCGCGTGATTCATCGCTTCAAACGCCATGCCTGCGGTa < 1:2648224/68‑1 (MQ=255)gaATCACCAGCATATCAGGACGGATATCGCCCGCGTGATTCATCGCTTCAAACGCCATGCCTGCGGTa < 1:200133/68‑1 (MQ=255)gaATCACCAGCATATCAGGACGGATATCGCCCGCGTGATTCATCGCTTCAAACGCCATGCCTGCGGTa < 1:1181711/68‑1 (MQ=255) aaTCACCAGCATATCAGGACGGATATCGCCCGCGTGATTCATCGCTTCAAACGCCATGCCTGCGGTAAt < 1:2078168/69‑1 (MQ=255) aaTCACCAGCATATCAGGACGGATATCGCCCGCGTGATTCATCGCTTCAAACGCCATGCCTGCGGTAAt < 1:1322261/69‑1 (MQ=255) aaTCACCAGCATATCAGGACGGATATCGCCCGCGTGATTCATCGCTTCAAACGCCATGCCTGCGGTAAt < 1:696622/69‑1 (MQ=255) aaTCACCAGCATATCAGGACGGATATCGCCCGCGTGATTCATCGCTTCAAACGCCATGCCTGCGGTAAt < 1:1945556/69‑1 (MQ=255) aaTCACCAGCATATCAGGACGGATATCGCCCGCGTGATTCATCGCTTCAAACGCCATGCCTGCGGTAAt < 1:3369225/69‑1 (MQ=255) aaTCACCAGCATATCAGGACGGATATCGCCCGCGTGATTCATCGCTTCAAACGCCATGCCTGCGGTAAt < 1:3030488/69‑1 (MQ=255) aaTCACCAGCATATCAGGACGGATATCGCCCGCGTGATTCATCGCTTCAAACGCCATGCCTGAGGTAAt < 1:3114/69‑1 (MQ=255) aaTCACCAGCATATCAGGACGGATATCGCCCGCGTGATTCATAGCTTCAAACGCCATGCCTGCGGTAAt < 1:2394490/69‑1 (MQ=255) tCACCAGCATATCAGGACGGATATCGCCCGCGTGATTCATCGCTTCAAACGCCATGCCTGCGGTAATcg > 1:1209742/1‑69 (MQ=255) tCACCAGCATATCAGGACGGATATCGCCCGCGTGATTCATCGCTTCAAACGCCATGCCTGCGGTAATcg > 1:2696494/1‑69 (MQ=255) tCACCAGCATATCAGGACGGATATCGCCCGCGTGATTCATCGCTTCAAACGCCATGCCTGCGGTAATcg > 1:680847/1‑69 (MQ=255) tCACCAGCATATCAGGACGGATATCGCCCGCGTGATTCATCGCTTCAAACGCCATGCCTGCGGTAATcg > 1:2880488/1‑69 (MQ=255) tCACAAGCATATCAGGACGGATATCGCCCGCGTGATTCATCGCTTCAAACGCCATGCCTGCGGTAATcg > 1:2562591/1‑69 (MQ=255) atatCAGGACGGATATCGCCCGCGTGATTCATCGCTTCAAACGCCATGCCTGCGGTAATCGCGCCATc > 1:2935466/1‑68 (MQ=255) atCAGGACGGATATCGCCCGCGTGATTCATCGCTTCAAACGCCATGCCAGCAGTAATCGCGCCATCGcc < 1:2777123/69‑1 (MQ=255) atCAGGACGGATATCGCCCGCGTGATTCATCGCTTCAAACGCCATGCCAGCAGTAATCGCGCCATCGcc < 1:2144143/69‑1 (MQ=255) atCAGGACGGATATCGCCCGCGTGATTCATCGCTTCAAACGCCATGCCAGCAGTAATCGCGCCATCGcc < 1:2658668/69‑1 (MQ=255) atCAGGACGGATATCGCCCGCGTGATTCATCGCTTCAAACGCCATGCCAGCAGTAATCGCGCCATCGcc < 1:1294855/69‑1 (MQ=255) atCAGGACGGATATCGCCCGCGTGATTCATCGCTTCAAACGCCATGCCAGCAGTAATCGCGCCATCGcc < 1:1455683/69‑1 (MQ=255) atCAGGACGGATATCGCCCGCGTGATTCATCGCTTCAAACGCCATGCCAGCAGTAATCGCGCCATCGcc < 1:1971506/69‑1 (MQ=255) atCAGGACGGATATCGCCCGCGTGATTCATCGCTTCAAACGCCATGCCAGCAGTAATCGCGCCATCGcc < 1:3119972/69‑1 (MQ=255) atCAGGACGGATATCGCCCGCGTGATTCATCGCTTCAAACGCCATGCCAGCAGTAATCGCGCCATCGcc < 1:339708/69‑1 (MQ=255) atCAGGACGGATATCGCCCGCGTGATTCATCGCTTCAAACGCCATGCCAGCAGTAATCGCGCCATCGcc < 1:1500059/69‑1 (MQ=255) atCAGGACGGATATCGCCCGCGTGATTCATCGCTTCAAACGCCATGCCAGCAGTAATCGCGCCATCGcc < 1:1931147/69‑1 (MQ=255) atCAGGACGGATATCGCCCGCGTGATTCATCGCTTCAAACGCCATGCCAGCAGTAATCGCGCCATCGcc < 1:2230558/69‑1 (MQ=255) atCAGGACGGATATCGCCCGCGTGATTCATCGCTTCAAACGCCATGCCAGCAGTAATCGCGCCATCGcc < 1:2269778/69‑1 (MQ=255) atCAGGACGGATATCGCCCGCGTGATTCATCGCTTCAAACGCCATGCCAGCAGTAATCGCGCCATCGcc < 1:157472/69‑1 (MQ=255) atCAGGACGGATATCGCCCGCGTGATTCATCGCTTCAAACGCCATGCCAGCAGTAATCGCGCCATCGcc < 1:2558311/69‑1 (MQ=255) tCAGGACGGATATCGCCCGCGTGATTCATCGCTTCAAACGCCATGCCTGCGGTAATCGCGCCATCGCCa > 1:411637/1‑69 (MQ=255) tCAGGACGGATATCGCCCGCGTGATTCATCGCTTCAAACGCCATGCCTGCGGTAATCGCGCCATCGCCa > 1:1658781/1‑69 (MQ=255) tCAGGACGGATATCGCCCGCGTGATTCATCGCTTCAAACGCCATGCCAGCAGTAATCGCGCCATCGcc < 1:2214173/68‑1 (MQ=255) tCAGGACGGATATCGCCCGCGTGATTCATCGCTTCAAACGCCATGCCAGCAGTAATCGCGCAATCGcc < 1:5835/68‑1 (MQ=255) acAGGACGGATATCGCCCGCGTGATTCATCGCTTCAAACGCCATGCCTCCGGTAATCGCGCCATCGcc > 1:1579847/2‑68 (MQ=255) gACGGATATCGCCCGCGTGATTCATCGCTTCAAACGCCATGCCAGCAGTAATCGCGCCATCGcc < 1:1857179/64‑1 (MQ=255) gATATCGCCCGCGTGATTCATCGCTTCAAACGCCATGCCTGCGGTAATCGCGCCATCGCCAATGAcaca < 1:2718183/69‑1 (MQ=255) gATATCGCCCGCGTGATTCATCGCTTCAAACGCCATGCCTGCGGTAATCGCGCCATCGCCAATGAcaca < 1:2961441/69‑1 (MQ=255) gATATCGCCCGCGTGATTCATCGCTTCAAACGCCATGCCTGCGGTAATCGCGCCATCGCCAATGAcaca < 1:491224/69‑1 (MQ=255) gATATCGCCCGCGTGATTCATCGCTTCAAACGCCATGCCTGCGGTAATCGCGCCATCGCCAATGAcaca < 1:563405/69‑1 (MQ=255) gATATCGCCCGCGTGATTCATCGCTTCAAACGCCATGCCTGCGGTAATCGCGCCATCGCCAATGAcaca < 1:2389247/69‑1 (MQ=255) gATATCGCCCGCGTGATTCATCGCTTCAAACGCCATGCCTGCGGTAATCGCGCCATCGCCAATGAcaca < 1:2382045/69‑1 (MQ=255) gATATCGCCCGCGTGATTCATCGCTTCAAACGCCATGCCTGCGGTAATCGCGCCATCGCCAATGAcaca < 1:1757211/69‑1 (MQ=255) gATATCGCCCGCGTGATTCATCGCTTCAAACGCCATGCCTGCGGTAATCGCGCCATCGCCAATGAcaca < 1:1370075/69‑1 (MQ=255) gATATCGCCCGCGTGATTCATCGCTTCAAACGCCATGCCTGCGGTAATCGCGCCATCGCCAATGAcaca < 1:2015426/69‑1 (MQ=255) gATATCGCCAGCGTGATTCATCGCTTCAAACGCCATGCCTGCGGTAATCGCGCCATCGCCAATGAcaca < 1:3159115/69‑1 (MQ=255) atatCGCCCGCGTGATTCATCGCTTCAAACGCCATGCCTGCGGTAATCGCGCCATCGCCAATGAcaca < 1:3029656/68‑1 (MQ=255) tatCGCCCTCGTGATTCATCGCTTCAAACGCCATGCCTGCGGTAATCGCGCCATCGCCAATTAcaca < 1:939121/67‑1 (MQ=255) tatCGCCCGCGTGATTCATCGCTTCAAACGCCATGCCTGCGGTAATCGCGCCATCGCCAATGACACAGa > 1:2997104/1‑69 (MQ=255) tatCGCCCGCGTGATTCATCGCTTCAAACGCCATGCCTGCGGTAATCGCGCCATCGCCAATGACACAGa > 1:1951564/1‑69 (MQ=255) tatCGCCCGCGTGATTCATCGCTTCAAACGCCATGCCTGCGGTAATCGCGCCATCGCCAATGACACAGa > 1:2142824/1‑69 (MQ=255) tatCGCCCGCGTGATTCATCGCTTCAAACGCCATGCCTGCGGTAATCGCGCCATCGCCAATGACACAGa > 1:1194318/1‑69 (MQ=255) tatCGCCCGCGTGATTCATCGCTTCAAACGCCATGCCTGCGGTAATCGCGCCATCGCCAATGACACAGa > 1:1155800/1‑69 (MQ=255) tatCGCCCGCGTGATTCATCGCTTCAAACGCCATGCCTGCGGTAATCGCGCCATCGCCAATGACACAGa > 1:728919/1‑69 (MQ=255) gCCCGCGTGATTCATCGCTTCAAACGCCATGCCTGCGGTAATCGCGCCATCGCCAATGACACAGACgg < 1:259835/68‑1 (MQ=255) tCATCGCTTCAAACGCCATGCCTGCGGTAATCGCGCCATCGcc < 1:2187294/43‑1 (MQ=255) tCATCGCTTCAAACGCCATGCCTGCGGTAATCGCGCCATCGCCAATGACACAGACGGTGCGGCGAtttt > 1:1284006/1‑69 (MQ=255) tCATCGCTTCAAACGCCATGCCTGCGGTAATCGCGCCATCGCCAATGACACAGACGGTGCGGCGAtttt > 1:1383728/1‑69 (MQ=255) tCATCGCTTCAAACGCCATGCCTGCGGTAATCGCGCCATCGCCAATGACACAGACGGTGCGGCGAtttt > 1:1572015/1‑69 (MQ=255) tCATCGCTTCAAACGCCATGCCTGCGGTAATCGCGCCATCGCCAATGACACAGACGGTGCGGCGAtttt > 1:3147898/1‑69 (MQ=255) tCATCGCTTCAAACGCCATGCCTGCGGTAATCGCGCCATCGCCAATGACACAGACGGTGCGGCGAtttt > 1:960921/1‑69 (MQ=255) tCATCGCTTCAAACGCCATGCCTGCGGTAATCGCGCCATCGCCAATGACACAGACGGTGCGGCGAtttt > 1:2541784/1‑69 (MQ=255) tCATCGCTTCAAACGCCATGCCTGCGGTAATCGCGCCATCGCCAATGACACAGACGGTGCGGCGAtttt > 1:1894896/1‑69 (MQ=255) tCATCGCTTCAAACGCCATGCCTGCGGTAATCGCGCCATCGCCAATGACACAGACGGTGCGGCGAtttt > 1:1936789/1‑69 (MQ=255) tCATCGCTTCAAACGCCATGCCTGCGGTAATCGCGCCATCGCCAATGACACAGACGGTGCGGCGAtttt > 1:2373594/1‑69 (MQ=255) ccATGCCAGCAGTAATCGCGCCATCGCCAATGACACAGAc > 1:1497113/1‑40 (MQ=38) ccATGCCAGCAGTAATCGCGCCATCGCCAATGACACAGACGGTGCGGCGATTTTTGCCTTCtttct > 1:2551747/1‑64 (MQ=255) ccATGCCAGCAGTAATCGCGCCATCGCCAATGACACAGACGGTGCGGCGATTTTTGCCTTCTTtctc > 1:2981963/1‑67 (MQ=255) ccATGCCAGCAGTAATCGCGCCATCGCCAATGACACAGACGGTGCGGCGATTTTTGCCTTCTTtctc > 1:861650/1‑67 (MQ=255) ccATGCCAGCAGTAATCGCGCCATCGCCAATGACACAGACGGTGCGGCGATTTTTGCCTTCTTtctc > 1:2568986/1‑67 (MQ=255) ccATGCCAGCAGTAATCGCGCCATCGCCAATGACACAGACGGTGCGGCGATTTTTGCCTTCTTtctc > 1:1428984/1‑67 (MQ=255) ccATGCCAGCAGTAATCGCGCCATCGCCAATGACACAGACGGTGCGGCGATTTTTGCCTTCTTtctc > 1:3412708/1‑67 (MQ=255) ccATGCCAGCAGTAATCGCGCCATCGCCAATGACACAGACGGTGCGGCGATTTTTGCCTTCTTtctc > 1:2829357/1‑67 (MQ=255) ccATGCCAGCAGTAATCGCGCCATCGCCAATGACACAGACGGTGCGGCGATTTTTGCCTTCTTtctc > 1:286157/1‑67 (MQ=255) ccATGCCAGCAGTAATCGCGCCATCGCCAATGACACAGACGGTGCGGCGATTTTTGCCTTCTTtctc > 1:1688146/1‑67 (MQ=255) ccATGCCAGCAGTAATCGCGCCATCGCCAATGACACAGACGGTGCGGCGATTTTTGCCTTCTTtctc > 1:2928912/1‑67 (MQ=255) ccATGCCAGCAGTAATCGCGCCATCGCCAATGACACAGACGGTGCGGCGATTTTTGCCTTCTTtctc > 1:1852844/1‑67 (MQ=255) ccATGCCAGCAGTAATCGCGCCATCGCCAATGACACAGACGGTGCGGCGATTTTAGCCTTCtttct > 1:2133944/1‑64 (MQ=255) ccTGCGGTAATCGCGCCATCGCCAATGACACAGACGGTGCGGCGAttttt > 1:2549868/1‑50 (MQ=255) ccTGCGGTAATCGCGCCATCGCCAATGACACAGACGGTGCGGCGATTTTTGCCTTCTTTTTCGgcagca > 1:2866782/1‑69 (MQ=255) ccTGCGGTAATCGCGCCATCGCCAATGACACAGACGGTGCGGCGATTTTTGCCTTCTTTTTCGgcagca > 1:2753088/1‑69 (MQ=255) ccTGCGGTAATCGCGCCATCGCCAATGACACAGACGGTGCGGCGATTTTTGCCTTCTTTTTCGgcagca > 1:1994897/1‑69 (MQ=255) ccTGCGGTAATCGCGCCATCGCCAATGACACAGACGGTGCGGCGATTTTTGCCTTCTTTTTCGgcagca > 1:2613191/1‑69 (MQ=255) ccTGCGGTAATCGCGCCATCGCCAATGACACAGACGGTGCGGCGATTTTTGCCTTCTTTTTCGgcagca > 1:1639507/1‑69 (MQ=255) ccTGCGGTAATCGCGCCATCGCCAATGACACAGACGGTGCGGCGATTTTTGCCTTCTTTTTCGgcagca > 1:85389/1‑69 (MQ=255) | GAATCACCAGCATATCAGGACGGATATCGCCCGCGTGATTCATCGCTTCAAACGCCATGCCTGCGGTAATCGCGCCATCGCCAATGACACAGACGGTGCGGCGATTTTTGCCTTCTTTTTCGGCAGCA > minE/268115‑268242 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |