| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 268,233 | T→C | 52.2% | E138E (GAA→GAG) | dxs ← | 1‑deoxyxylulose‑5‑phosphate synthase, thiamine‑requiring, FAD‑requiring |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 268,233 | 0 | T | C | 52.2% | 3.9 / 58.1 | 69 | E138E (GAA→GAG) | dxs | 1‑deoxyxylulose‑5‑phosphate synthase, thiamine‑requiring, FAD‑requiring |
| Reads supporting (aligned to +/- strand): ref base T (18/15); new base C (27/9); total (45/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.42e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCATGCCTGCGGTAATCGCGCCATCGCCAATGACACAGACGGTGCGGCGATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATAC > minE/268169‑268298 | ccATGCCAGCAGTAATCGCGCCATCGCCAATGACACAGACGGTGCGGCGATTTTTGCCTTCTTtctc > 1:2981963/1‑67 (MQ=255)ccATGCCAGCAGTAATCGCGCCATCGCCAATGACACAGACGGTGCGGCGATTTTTGCCTTCTTtctc > 1:2829357/1‑67 (MQ=255)ccATGCCAGCAGTAATCGCGCCATCGCCAATGACACAGACGGTGCGGCGATTTTTGCCTTCTTtctc > 1:286157/1‑67 (MQ=255)ccATGCCAGCAGTAATCGCGCCATCGCCAATGACACAGACGGTGCGGCGATTTTTGCCTTCTTtctc > 1:1688146/1‑67 (MQ=255)ccATGCCAGCAGTAATCGCGCCATCGCCAATGACACAGACGGTGCGGCGATTTTTGCCTTCTTtctc > 1:2928912/1‑67 (MQ=255)ccATGCCAGCAGTAATCGCGCCATCGCCAATGACACAGACGGTGCGGCGATTTTTGCCTTCTTtctc > 1:1852844/1‑67 (MQ=255)ccATGCCAGCAGTAATCGCGCCATCGCCAATGACACAGACGGTGCGGCGATTTTTGCCTTCTTtctc > 1:1428984/1‑67 (MQ=255)ccATGCCAGCAGTAATCGCGCCATCGCCAATGACACAGACGGTGCGGCGATTTTTGCCTTCTTtctc > 1:3412708/1‑67 (MQ=255)ccATGCCAGCAGTAATCGCGCCATCGCCAATGACACAGACGGTGCGGCGATTTTTGCCTTCTTtctc > 1:861650/1‑67 (MQ=255)ccATGCCAGCAGTAATCGCGCCATCGCCAATGACACAGACGGTGCGGCGATTTTTGCCTTCTTtctc > 1:2568986/1‑67 (MQ=255) ccTGCGGTAATCGCGCCATCGCCAATGACACAGACGGTGCGGCGATTTTTGCCTTCTTTTTCGgcagca > 1:2753088/1‑69 (MQ=255) ccTGCGGTAATCGCGCCATCGCCAATGACACAGACGGTGCGGCGATTTTTGCCTTCTTTTTCGgcagca > 1:1994897/1‑69 (MQ=255) ccTGCGGTAATCGCGCCATCGCCAATGACACAGACGGTGCGGCGATTTTTGCCTTCTTTTTCGgcagca > 1:2613191/1‑69 (MQ=255) ccTGCGGTAATCGCGCCATCGCCAATGACACAGACGGTGCGGCGATTTTTGCCTTCTTTTTCGgcagca > 1:1639507/1‑69 (MQ=255) ccTGCGGTAATCGCGCCATCGCCAATGACACAGACGGTGCGGCGATTTTTGCCTTCTTTTTCGgcagca > 1:2866782/1‑69 (MQ=255) ccTGCGGTAATCGCGCCATCGCCAATGACACAGACGGTGCGGCGATTTTTGCCTTCTTTTTCGgcagca > 1:85389/1‑69 (MQ=255) gTAATCGCTCCATCGCCAATGACACAGACGGTGCGGCGATTTTTGCCTTCTTTTTCGGCAGCAACCGc < 1:2716174/68‑1 (MQ=255) gTAATCGCGCCATCGCCAATGACACAGACGGTGCGGCGATTTTTGCCTTCTTTTTCGGCAGCAACCGc < 1:2402753/68‑1 (MQ=255) gTAATCGCGCCATCGCCAATGACACAGACGGTGCGGCGATTTTTGCCTTCTTTTTCGGCAGCAACCGc < 1:41694/68‑1 (MQ=255) tAATCGCGCCATCGCCAATGACACAGACGGTGCGGCGATTTTTGCCTTCTTTTTCGGCAGCAACCGCa < 1:623854/68‑1 (MQ=255) aaTCGCGCCATCGCCAATGACACAGACGGTGCGGCGATTTTTGCCTTCTTTTTCGGCAGCAACCGCAAt > 1:2944143/1‑69 (MQ=255) cgcgCCATCGCCAATGACACAGACGGTGCGGCGATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATAcc < 1:934551/69‑1 (MQ=255) gcCATCGCCAATGACACAGACGGTGCGGCGATTTTTGCCTTCTTTCTCGGCAGCAACTGCGATCCCAAt < 1:3163126/69‑1 (MQ=255) gcCATCGCCAATGACACAGACGGTGCGGCGATTTTTGCCTTCTTTCTCGGCAGCAACTGCGATCCCAAt < 1:564752/69‑1 (MQ=255) gcCATCGCCAATGACACAGACGGTGCGGCGATTTTTGCCTTCTTTCTCGGCAGCAACTGCGATCCCAAt < 1:2536042/69‑1 (MQ=255) ccATCGCCAATGACACAGACGGTGCGGCGATTTTTGCCTTCTTTCTCGGCAGCAACTGCGATCCCAAt < 1:2365986/68‑1 (MQ=255) gCCAATGACACAGACGGTGCGGCTATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCgg > 1:204622/1‑68 (MQ=255) gCCAATGACACAGACGGTGCGGCGATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCgg > 1:156228/1‑68 (MQ=255) gCCAATGACACAGACGGTGCGGCGATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCgg > 1:1747242/1‑68 (MQ=255) gCCAATGACACAGACGGTGCGGCGATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCgg < 1:3149840/68‑1 (MQ=255) gCCAATGACACAGACGGTGCGGCGATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCgg < 1:1997009/68‑1 (MQ=255) gCCAATGACACAGACGGTGCGGCGATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCgg < 1:1003877/68‑1 (MQ=255) gCCAATGACACAGACGGTGCGGCGATTTTTGCCTTCTTTCTCgg > 1:628101/1‑44 (MQ=255) gCCAATGACACAGACGGTGCGGCGATTTTTGCCTTCTTTCTCgg > 1:1581571/1‑44 (MQ=255) gCCAATGACACAGACGGTGCGGCGATTTTTGCCTTCTTTCTCgg > 1:3093009/1‑44 (MQ=255) gCCAATGACACAGACGGTGCGGCGATTTTTGCCTTCTTTCTCgg > 1:2785388/1‑44 (MQ=255) gCCAATGACACAGACGGTGCGGCGATTTTTGCCTTCTTTCTCgg > 1:1333985/1‑44 (MQ=255) gCCAATGACACAGACGGTGCGGCGATTTTTGCCTTCTTTCTCgg > 1:1218990/1‑44 (MQ=255) gCCAATGACACAGACGGTGCGGCGATTTTTGCCTTCTTTCTCgg > 1:2677087/1‑44 (MQ=255) gCCAATGACACAGACGGTGCGGCGATTTTTGCCTTCTTTCTCgg > 1:201929/1‑44 (MQ=255) gCCAATGACACAGACGGTGCGGCGATTTTTGCCTTCTTTCTCgg > 1:2511824/1‑44 (MQ=255) gCCAATGACACAGACGGTGCGGCGATTTTTGCCTTCTTTCTCgg > 1:1053719/1‑44 (MQ=255) gCCAATGACACAGACGGTGCGGCGATTTTTGCCTTCTTTCTCgg > 1:711874/1‑44 (MQ=255) gCCAATGACACAGACGGTGCGGCGATTTTTGCCTTCTTTCTCgg > 1:2367097/1‑44 (MQ=255) gCCAATGACACAGACGGTGCGGCGATTTTTGCCTTCTTTCTCgg > 1:2099293/1‑44 (MQ=255) gCCAATGACACAGACGGTGCGGCGATTTTTGCCTTCTTTCTCgg > 1:2340660/1‑44 (MQ=255) gCCAATGACACAGACGGTGCGGCGATTTTTGCCTTCTTTCTCgg > 1:2106411/1‑44 (MQ=255) gCCAATGACACAGACGGTGCGGCGATTTTTGCCTTCTTTCTCgg > 1:2247982/1‑44 (MQ=255) gCCAATGACACAGACGGTGCGGCGATTTTTGCCTTCTTTCTCgg > 1:682532/1‑44 (MQ=255) gCCAATGACACAGACGGTGCGGCGATTTTTGCCTTCTTTCTCgg > 1:1161782/1‑44 (MQ=255) gCCAATGACACAGACGGTGCGGCGATTTTTGCCTTCTTTCTCgg > 1:115968/1‑44 (MQ=255) gCCAATGACACAGACGGTGCGGCGATTTTTGCCTTATTTTTCGGCAGCAACCGCAATACCAATTCCgg < 1:2153394/68‑1 (MQ=255) gCCAATGACACAGACGGTGCGGCGATTTTTGCCTTATTTCTCgg > 1:1281485/1‑44 (MQ=38) cccAATGACACAGACGGTGCCGCGATTTTTGCCTTCTTTCTCgg > 1:2247642/2‑44 (MQ=38) aaTGACACAGACGGTGCGGCGATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACt < 1:26749/69‑1 (MQ=255) aaTGACACAGACGGTGCGGCGATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACt < 1:1350571/69‑1 (MQ=255) aaTGACACAGACGGTGCGGCGATTTTTGCCTTCTTTCTCGGCAGCAACTGCGATCCCAAt > 1:2067511/1‑60 (MQ=255) aaTGACACAGACGGTGCGGCGATTTTTGCCTTCTTTCTCGGCAGCAACTGCGATCCCAATTCCGGCACt > 1:859591/1‑69 (MQ=255) aaTGACACAGACGGTGCGGCGATTTTTGCCTTCTTTCTCGGCAGCAACTGCGATCCCAATTCCGGCACt > 1:813747/1‑69 (MQ=255) aaTGACACAGACGGTGCGGCGATTTTTGCCTTCTTTCTCGGCAGCAACTGCGATCCCAATTCCGGCACt > 1:1653720/1‑69 (MQ=255) aaTGACACAGACGGTGCGGCGATTTTTGCCTTCTTTCTCGGCAGCAACTGCGATCCCAATTCCGGCACt > 1:2913380/1‑69 (MQ=255) aaTGACACAGACGGTGCGGCGATTTTTGCCTTCTTTCTCGGCAGCAACTGCGATCCCAATTCCGGCACt > 1:2348271/1‑69 (MQ=255) gACACAGACGGTGCGGCGATTTTTGCCTTGTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGa > 1:2904175/1‑68 (MQ=255) gACACAGACGGTGCGGCGATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGa > 1:2089793/1‑68 (MQ=255) gACACAGACGGTGCGGCGATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGa > 1:2172970/1‑68 (MQ=255) gACACAGACGGTGCGGCGATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGa > 1:2387713/1‑68 (MQ=255) gACACAGACGGTGCGGCGATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGa > 1:484887/1‑68 (MQ=255) gACACAGACGGTGCGGCGATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGa > 1:3330574/1‑68 (MQ=255) gACACAGACGGTGCGGCGATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGa > 1:3246796/1‑68 (MQ=255) gACACAGACGGTGCGGCGATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGa > 1:1901467/1‑68 (MQ=255) tGCGGCGATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTtgatg < 1:3367076/69‑1 (MQ=255) tGCGGCGATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTtgatg < 1:3368578/69‑1 (MQ=255) tGCGGCGATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTtgatg < 1:3370984/69‑1 (MQ=255) gcgATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCgg < 1:2680659/47‑1 (MQ=255) gcgATTTTTGCCTTCTTTCTCGGCAGCAACTGCGATCCCAATTCCGGCACTGATGGAGGTTGATGAAt < 1:126922/68‑1 (MQ=255) gcgATTTTTGCCTTCTTTCTCGGCAGCAACTGCGATCCCAATTCCGGCACTGATGGAGGTTGATGAAt < 1:3085491/68‑1 (MQ=255) gcgATTTTTGCCTTCTTTCTCGGCAGCAACTGCGATCCCAATTCCGGCACTGATGGAGGTTGATGAAt < 1:2679693/68‑1 (MQ=255) gcgATTTTTGCCTTCTTTCTCGGCAGCAACTGCGATCCCAATTCCGGCACTGATGGAGGTTGATGAAt < 1:2473658/68‑1 (MQ=255) gcgATTTTTGCCTTCTTTCTCGGCAGCAACTGCGATCCCAATTCCGGCACTGATGGAGGTTGATGAAt < 1:2827641/68‑1 (MQ=255) ttctttctCGGCAGCAACTGCGATCCCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTaa > 1:1974350/1‑69 (MQ=255) ttctttctCGGCAGCAACTGCGATCCCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTaa > 1:908564/1‑69 (MQ=255) ttctttctCGGCAGCAACTGCGATCCCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTaa > 1:2185265/1‑69 (MQ=255) ttctttctCGGCAGCAACTGCGATCCCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTaa > 1:602324/1‑69 (MQ=255) ttctttctCGGCAGCAACTGCGATCCCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTaa > 1:3156395/1‑69 (MQ=255) ttttCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATAc < 1:740743/68‑1 (MQ=255) ttttCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATAc < 1:2422566/68‑1 (MQ=255) ttttCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATAc < 1:2212631/68‑1 (MQ=255) ttttCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATAc < 1:1253161/68‑1 (MQ=255) ttttCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATAc < 1:1737907/68‑1 (MQ=255) | CCATGCCTGCGGTAATCGCGCCATCGCCAATGACACAGACGGTGCGGCGATTTTTGCCTTCTTTTTCGGCAGCAACCGCAATACCAATTCCGGCACTGATGGAGGTTGATGAATGCCCGACGCTTAATAC > minE/268169‑268298 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |