| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 31,416 | C→T | 67.6% | T974T (ACC→ACT) | carB → | carbamoyl‑phosphate synthase large subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 31,416 | 0 | C | T | 67.6% | 63.9 / 41.5 | 80 | T974T (ACC→ACT) | carB | carbamoyl‑phosphate synthase large subunit |
| Reads supporting (aligned to +/- strand): ref base C (22/4); new base T (24/30); total (46/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.94e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.37e-01 | |||||||||||
AAAGAACGCGTGGTGGACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCCGCGTCTGGTAAACAAGGTGCATG > minE/31354‑31480 | aaaGAACGCGTGGTGGACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACCcacgg > 1:3384783/1‑68 (MQ=255)aaaGAACGCGTGGTGGACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACCcacgg > 1:433586/1‑68 (MQ=255)aaaGAACGCGTGGTGGACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACCcacgg > 1:2845205/1‑68 (MQ=255)aaaGAACGCGTGGTGGACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACCcacgg > 1:434272/1‑68 (MQ=255)aaaGAACGCGTGGTGGACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACCcacgg > 1:2634863/1‑68 (MQ=255)aaaGAACGCGTGGTGGACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACCcacgg < 1:3334530/68‑1 (MQ=255)aaaGAACGCGTGGTGGACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGATTGATGCGACCcacgg > 1:2779726/1‑68 (MQ=255)aaaGAACGCGTGGTGGACCTGGCGG‑AAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACCcacgg > 1:1606420/1‑67 (MQ=255) cgggtggtgGACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACTCACGGCACGGCg > 1:3389222/4‑69 (MQ=255) cgcgTGGTGGACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGCGCTGGATGCGACTCACGGCACGGCg > 1:2887736/1‑69 (MQ=255) cgcgTGGTGGACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACTcaccgcac > 1:3285493/1‑65 (MQ=255) cgcgTGGTGGACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACTCacggcacggc > 1:795733/1‑68 (MQ=255) cgcgTGGTGGACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACTCACGGCATGGCg > 1:1563905/1‑69 (MQ=255) cgcgTGGTGGACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACTCACGGCACGGCg > 1:2457541/1‑69 (MQ=255) cgcgTGGTGGACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACTCACGGCACGGCg > 1:3404818/1‑69 (MQ=255) cgcgTGGTGGACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACTCACGGCACGGCg > 1:2913330/1‑69 (MQ=255) cgcgTGGTGGACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACTCACGGCACGGCg > 1:1101182/1‑69 (MQ=255) cgcgTGGTGGACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACTCACGGCACGGCg > 1:1240252/1‑69 (MQ=255) cgcgTGGTGGACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACTCACGGCACGGCg > 1:2769294/1‑69 (MQ=255) cgcgTGGTGGACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACTCACGGCACGGCg > 1:2760803/1‑69 (MQ=255) cgcgTGGTGGACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACTCACGGCACGGCg > 1:1402644/1‑69 (MQ=255) cgcgTGGTGGACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACTCACGGCACGGCg > 1:436596/1‑69 (MQ=255) cgcgTGGTGGACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACTCACGGCACGGCg > 1:1402768/1‑69 (MQ=255) cgcgTGGTGGACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACTCACGGCACGGCg > 1:480073/1‑69 (MQ=255) cgcgTGGTGGACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACTCACGGCACGGCg > 1:808226/1‑69 (MQ=255) cgcgTGGTGGACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACTCACGGCACGGCg > 1:698631/1‑69 (MQ=255) cgcgTGGTGGACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACTCACGGCACGGCg > 1:1776429/1‑69 (MQ=255) cgcgTGGTGGACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACTCACGGCACGGCg > 1:794769/1‑69 (MQ=255) cgcgTGGTGGACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACTCACGGCACGGCg > 1:2055757/1‑69 (MQ=255) cgcgTGGTGGACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACTCACGGCACGGCg > 1:3269411/1‑69 (MQ=255) cgcgTGGTGGACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACTCACGGCACGGCg > 1:743440/1‑69 (MQ=255) cgcgTGGTGGACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACTCACGGCACGGCg > 1:744515/1‑69 (MQ=255) gtggACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATtgtg > 1:2508204/1‑69 (MQ=255) ggcAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACTCACGGCACGGCGATTGTGCTGGGCGaa < 1:1338208/67‑1 (MQ=255) ggcAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACTCACGGCACGGCGATTGTGCTGGGCGaa < 1:1140652/67‑1 (MQ=255) ggcAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACTCACGGCACGGCGATTGTGCTGGGCGaa < 1:3307324/67‑1 (MQ=255) aCTGCTGAAACGGGGCTTCGAGCTGGATGCGACTCACGGCACGGCGATtg < 1:727996/50‑1 (MQ=255) aCTGCTGAAACAGGGCTTCGAGCTTGATGCGACTCACGGCACGTCGATtg < 1:1854463/50‑1 (MQ=255) aCTGCTGAAACAGGGCTTCGAGCTTGATGCGACTCACGGCACGGCGATtg < 1:3283270/50‑1 (MQ=255) aCTGCTGAAACAGGGCTTCGAGCTGGATGCGACTCACGGCACGGCTATtg < 1:2854038/50‑1 (MQ=255) aCTGCTGAAACAGGGCTTCGAGCTGGATGCGACTCACGGCACGGCGATtg < 1:336696/50‑1 (MQ=255) aCTGCTGAAACAGGGCTTCGAGCTGGATGCGACTCACGGCACGGCGATtg < 1:507503/50‑1 (MQ=255) aCTGCTGAAACAGGGCTTCGAGCTGGATGCGACTCACGGCACGGCGATtg < 1:372365/50‑1 (MQ=255) aCTGCTGAAACAGGGCTTCGAGCTGGATGCGACTCACGGCACGGCGATtg < 1:2922914/50‑1 (MQ=255) aCTGCTGAAACAGGGCTTCGAGCTGGATGCGACTCACGGCACGGCGATtg < 1:516526/50‑1 (MQ=255) aCTGCTGAAACAGGGCTTCGAGCTGGATGCGACTCACGGCACGGCGATtg < 1:736495/50‑1 (MQ=255) aCTGCTGAAACAGGGCTTCGAGCTGGATGCGACTCACGGCACGGCGATtg < 1:742086/50‑1 (MQ=255) aCTGCTGAAACAGGGCTTCGAGCTGGATGCGACTCACGGCACGGCGATtg < 1:895290/50‑1 (MQ=255) aCTGCTGAAACAGGGCTTCGAGCTGGATGCGACTCACGGCACGGCGATtg < 1:90003/50‑1 (MQ=255) aCTGCTGAAACAGGGCTTCGAGCTGGATGCGACTCACGGCACGGCGATtg < 1:1963454/50‑1 (MQ=255) aCTGCTGAAACAGGGCTTCGAGCTGGATGCGACTCACGGCACGGCGATtg < 1:3062381/50‑1 (MQ=255) aCTGCTGAAACAGGGCTTCGAGCTGGATGCGACTCACGGCACGGCGATtg < 1:2998552/50‑1 (MQ=255) aCTGCTGAAACAGGGCTTCGAGCTGGATGCGACTCACGGCACGGCGATtg < 1:2295919/50‑1 (MQ=255) aCTGCTGAAACAGGGCTTCGAGCTGGATGCGACTCACGGCACGGCGATtg < 1:1048186/50‑1 (MQ=255) aCTGCTGAAACAGGGCTTCGAGCTGGATGCGACTCACGGCACGGCGATtg < 1:2863267/50‑1 (MQ=255) aCTGCTGAAACAGGGCTTCGAGCTGGATGCGACTCACGGCACGGCGATtg < 1:1862342/50‑1 (MQ=255) aCTGCTGAAACAGGGCTTCGAGCTGGATGCGACTCACGGCACGGCGATtg < 1:1729101/50‑1 (MQ=255) aCTGCTGAAACAGGGCTTCGAGCTGGATGCGACTCACGGCACGGCGATtg < 1:268582/50‑1 (MQ=255) aCTGCTGAAACAGGGCTTCGAGCTGGATGCGACTCACGGCACGGCGATtg < 1:2645507/50‑1 (MQ=255) aCTGCTGAAACAGGGCTTCGAGCTGGATGCGACTCACGGCACGGCGATtg < 1:147629/50‑1 (MQ=255) aCTGCTGAAACAGGGCTTCGAGCTGGATGCGACTCACGGCACGGCGATtg < 1:2421308/50‑1 (MQ=255) aCTGCTGAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTa > 1:2026479/1‑68 (MQ=255) aCTGCTGAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTa > 1:2946512/1‑68 (MQ=255) ctgctgAAACAGGGCTTCGAGCTGGATGCGACTCACGGCACGGCGATtg < 1:1197625/49‑1 (MQ=255) gggCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCCGCGTCt > 1:2848336/1‑69 (MQ=255) gggCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCCGCGTCt > 1:523341/1‑69 (MQ=255) gggCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCCGCGTCt > 1:670362/1‑69 (MQ=255) gggCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCCGCGTCt > 1:676642/1‑69 (MQ=255) gCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCCGCGTCTg > 1:554553/1‑68 (MQ=255) gCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCCGCGTCTg > 1:2169400/1‑68 (MQ=255) gCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCCGCGTCTg > 1:1074792/1‑68 (MQ=255) gCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCCGCGTCTg > 1:732651/1‑68 (MQ=255) gCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCCGCGTCTg > 1:724455/1‑68 (MQ=255) gCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCCGCGTCTg > 1:3249906/1‑68 (MQ=255) gCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCCGCGTCTg > 1:2442082/1‑68 (MQ=255) gCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCCGCGTCTg > 1:3176312/1‑68 (MQ=255) cTTCGAGCTGGATGCGACTCACGGCACGGCGATtg < 1:2411517/35‑1 (MQ=255) cGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCCGCGTCTGGTAAACAAGGTGCATg < 1:222353/69‑1 (MQ=255) cGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCCGCGTCTGGTAAACAAGGTGCATg < 1:1996864/69‑1 (MQ=255) cGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCCGCGTCTGGTAAACAAGGTGCATg < 1:40774/69‑1 (MQ=255) | AAAGAACGCGTGGTGGACCTGGCGGCAAAACTGCTGAAACAGGGCTTCGAGCTGGATGCGACCCACGGCACGGCGATTGTGCTGGGCGAAGCAGGTATCAACCCGCGTCTGGTAAACAAGGTGCATG > minE/31354‑31480 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |