| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 271,052 | T→C | 23.7% | G345G (GGT→GGC) | thiI → | sulfurtransferase required for thiamine and 4‑thiouridine biosynthesis |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 271,052 | 0 | T | C | 23.7% | 77.8 / 19.2 | 63 | G345G (GGT→GGC) | thiI | sulfurtransferase required for thiamine and 4‑thiouridine biosynthesis |
| Reads supporting (aligned to +/- strand): ref base T (28/20); new base C (6/9); total (34/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.47e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.28e-01 | |||||||||||
CACATCATCAACCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAAGAAGAGAAGTTCG > minE/270984‑271119 | cacaTCATCAACCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTgtggt > 1:1757668/1‑69 (MQ=255)cacaTCATCAACCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTgtggt > 1:1701092/1‑69 (MQ=255)cacaTCATCAACCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTgtggt > 1:2863305/1‑69 (MQ=255)cacaTCATCAACCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTgtggt > 1:2732279/1‑69 (MQ=255) atcatcAACCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGTGGtgtg < 1:1383826/69‑1 (MQ=255) atcatcAACCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGTGGtgtg < 1:1606750/69‑1 (MQ=255) atcatcAACCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGTGGtgtg < 1:298427/69‑1 (MQ=255) atcatcAACCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGTGGtgtg < 1:1493269/69‑1 (MQ=255) atcatcAACCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGTGGtgtg < 1:2907126/69‑1 (MQ=255) atcatcAACCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGTGGtgtg < 1:746134/69‑1 (MQ=255) atcatcAACCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGTGGtgtg < 1:6402/69‑1 (MQ=255) atcatcAACCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGTGGtgtg < 1:2014219/69‑1 (MQ=255) atcatcAACCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGTGGtgtg < 1:1684053/69‑1 (MQ=255) atcatcAACCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGTGGtgtg < 1:977874/69‑1 (MQ=255) atcatcAACCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGTGGtgtg < 1:1711741/69‑1 (MQ=255) atcatcAACCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGTGGtgtg < 1:216965/69‑1 (MQ=255) atcaACCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGAtc > 1:273537/1‑69 (MQ=255) atcaACCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGAtc > 1:1725845/1‑69 (MQ=255) atcaACCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGAtc > 1:1575951/1‑69 (MQ=255) atcaACCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGAtc > 1:601046/1‑69 (MQ=255) aCCGAAGACTTTGCCCGCACGATGCCGGAATATTGCGGCGTGATTTCCAAAAGCCCGACGGTGaaaacg < 1:2207785/69‑4 (MQ=255) aCCGAAGACTTTGCCCGCACGATGCCGGAATATTGCGGCGTGATTTCCAAAAGCCCGACGGTGAAAGcg < 1:1871534/69‑2 (MQ=255) aCCGAAGACTTTGCCCGCACGATGCCGGAATATTGCGGCGTGATTTCCAAAAGCCCGACGGTGAAAGcg < 1:586771/69‑2 (MQ=255) aCCGAAGACTTTGCCCGCACGATGCCGGAATATTGCGGCGTGATTTCCAAAAGCCCGACGGTGAAAGcg < 1:1972464/69‑2 (MQ=255) aCCGAAGACTTTGCCCGCACGATGCCGGAATATTGCGGCGTGATTTCCAAAAGCCCGACGGTGAAAGcg < 1:2041604/69‑2 (MQ=255) aCCGAAGACTTTGCCCGCACGATGCCGGAATATTGCGGCGTGATTTCCAAAAGCCCGACGGTGAAAGcg < 1:2097563/69‑2 (MQ=255) aCCGAAGACTTTGCCCGCACGATGCCGGAATATTGCGGCGTGATTTCCAAAAGCCCGACGGTGAAAGcg < 1:173271/69‑2 (MQ=255) aCCGAAGACTTTGCCCGCACGATGCCGGAATATTGCGGCGTGATTTCCAAAAGCCCGACGGTGAAAGcg < 1:2973753/69‑2 (MQ=255) aCCGAAGACTTTGCCCGCACGATGCCGGAATATTGCGGCGTGATTTCCAAAAGCCCGACGGTGAAAGcg < 1:1573960/69‑2 (MQ=255) gAAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGt > 1:2209153/1‑68 (MQ=255) gAAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGt > 1:1153071/1‑68 (MQ=255) gAAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGt > 1:3134418/1‑68 (MQ=255) gAAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGt > 1:538427/1‑68 (MQ=255) gAAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGt > 1:2819559/1‑68 (MQ=255) gAAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGt > 1:3372047/1‑68 (MQ=255) gAAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGt > 1:1421980/1‑68 (MQ=255) gAAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGt > 1:14534/1‑68 (MQ=255) gAAGACTTTGCTCGCACGATGCCGGAATATTGAGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGt > 1:3069275/1‑68 (MQ=255) aGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTaa < 1:3076905/69‑1 (MQ=255) aGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTaa < 1:2067669/69‑1 (MQ=255) aGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTaa < 1:1423431/69‑1 (MQ=255) aGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTaa < 1:2097588/69‑1 (MQ=255) aGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTaa < 1:1178106/69‑1 (MQ=255) aGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTaa < 1:997718/69‑1 (MQ=255) gACTTTGCTCGCACGATGCCGGAATATTGTGGtgtg < 1:3124036/36‑1 (MQ=255) gACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGAc < 1:3296063/53‑1 (MQ=255) gACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGAc < 1:2220979/53‑1 (MQ=255) gACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGAc < 1:63537/53‑1 (MQ=255) gACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTaa < 1:2231866/68‑1 (MQ=255) cTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGaa < 1:1245701/67‑1 (MQ=255) gCACGATGCCGGAATATTGCGGCGTGATTTCCAAAAGCCCGACGGTGaa > 1:1334117/1‑49 (MQ=255) gCACGATGCCGGAATATTGCGGCGTGATTTCCAAAAGCCCGACGGTGAAAGCGGTTAAATCGAAGAt > 1:1615125/1‑67 (MQ=255) gCACGATGCCGGAATATTGCGGCGTGATTTCCAAAAGCCCGACGGTGAAAGCGGTTAAATCGAAGAt > 1:875763/1‑67 (MQ=255) gCACGATGCCGGAATATTGCGGCGTGATTTCCAAAAGCCCGACGGTGAAAGCGGTTAAATCGAAGAt > 1:1607771/1‑67 (MQ=255) gCACGATGCCGGAATATTGCGGCGTGATTTCCAAAAGCCCGACGGTGAAAGCGGTTAAATCGAAGAt > 1:1968003/1‑67 (MQ=255) gCACGATGCCGGAATATTGCGGCGTGATTTCCAAAAGCCCGACGGTGAAAGCGGTTAAATCGAAGAt > 1:2504950/1‑67 (MQ=255) ggAATATTGTGGTGTTATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGgaag < 1:1232000/68‑1 (MQ=255) ggAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGgaag < 1:1654374/68‑1 (MQ=255) ggAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGgaag < 1:364317/68‑1 (MQ=255) ggAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGgaag < 1:1872466/68‑1 (MQ=255) ggAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGgaag < 1:1404595/68‑1 (MQ=255) ggAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGgaag < 1:3043280/68‑1 (MQ=255) ggAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGgaag < 1:2613056/68‑1 (MQ=255) ttGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAagaagagaa > 1:7852/1‑69 (MQ=255) ttGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAagaagagaa > 1:756310/1‑69 (MQ=255) ttGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAagaagagaa > 1:3385502/1‑69 (MQ=255) ttGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAagaagagaa > 1:2139018/1‑69 (MQ=255) ttGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAagaagagaa > 1:303766/1‑69 (MQ=255) ttGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAagaagagaa > 1:3025362/1‑69 (MQ=255) ttGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAagaagagaa > 1:2976152/1‑69 (MQ=255) ttGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAagaagagaa > 1:228465/1‑69 (MQ=255) ttGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAagaagagaa > 1:1049347/1‑69 (MQ=255) ttGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAagaagagaa > 1:2064556/1‑69 (MQ=255) ttGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAagaagagaa > 1:1924980/1‑69 (MQ=255) ttGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAagaagagaa > 1:1703369/1‑69 (MQ=255) ttGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAagaagagaa > 1:1332081/1‑69 (MQ=255) ttGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAagaagagaa > 1:1218746/1‑69 (MQ=255) ttGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAagaagaga < 1:2572847/68‑1 (MQ=255) ttGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAagaagaga < 1:1650191/68‑1 (MQ=255) ttGTGGTGTGATCTACAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAagaagagaa > 1:1927161/1‑69 (MQ=255) gtgtGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAAGAAGAGAAGTTCg > 1:736546/1‑69 (MQ=255) gtgtGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAAGAAGAGAAGTTCg > 1:1104579/1‑69 (MQ=255) | CACATCATCAACCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAAGAAGAGAAGTTCG > minE/270984‑271119 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |