| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 271,058 | C→T | 22.2% | I347I (ATC→ATT) | thiI → | sulfurtransferase required for thiamine and 4‑thiouridine biosynthesis |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 271,058 | 0 | C | T | 22.2% | 90.9 / 18.0 | 67 | I347I (ATC→ATT) | thiI | sulfurtransferase required for thiamine and 4‑thiouridine biosynthesis |
| Reads supporting (aligned to +/- strand): ref base C (32/20); new base T (6/9); total (38/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.54e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01 | |||||||||||
ATCAACCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAAGAAGAGAAGTTCGACTTCAG > minE/270990‑271126 | atcaACCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGAtc > 1:601046/1‑69 (MQ=255)atcaACCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGAtc > 1:1725845/1‑69 (MQ=255)atcaACCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGAtc > 1:273537/1‑69 (MQ=255)atcaACCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGAtc > 1:1575951/1‑69 (MQ=255) aCCGAAGACTTTGCCCGCACGATGCCGGAATATTGCGGCGTGATTTCCAAAAGCCCGACGGTGaaaacg < 1:2207785/69‑4 (MQ=255) aCCGAAGACTTTGCCCGCACGATGCCGGAATATTGCGGCGTGATTTCCAAAAGCCCGACGGTGAAAGcg < 1:1871534/69‑2 (MQ=255) aCCGAAGACTTTGCCCGCACGATGCCGGAATATTGCGGCGTGATTTCCAAAAGCCCGACGGTGAAAGcg < 1:1972464/69‑2 (MQ=255) aCCGAAGACTTTGCCCGCACGATGCCGGAATATTGCGGCGTGATTTCCAAAAGCCCGACGGTGAAAGcg < 1:173271/69‑2 (MQ=255) aCCGAAGACTTTGCCCGCACGATGCCGGAATATTGCGGCGTGATTTCCAAAAGCCCGACGGTGAAAGcg < 1:2041604/69‑2 (MQ=255) aCCGAAGACTTTGCCCGCACGATGCCGGAATATTGCGGCGTGATTTCCAAAAGCCCGACGGTGAAAGcg < 1:2097563/69‑2 (MQ=255) aCCGAAGACTTTGCCCGCACGATGCCGGAATATTGCGGCGTGATTTCCAAAAGCCCGACGGTGAAAGcg < 1:1573960/69‑2 (MQ=255) aCCGAAGACTTTGCCCGCACGATGCCGGAATATTGCGGCGTGATTTCCAAAAGCCCGACGGTGAAAGcg < 1:2973753/69‑2 (MQ=255) aCCGAAGACTTTGCCCGCACGATGCCGGAATATTGCGGCGTGATTTCCAAAAGCCCGACGGTGAAAGcg < 1:586771/69‑2 (MQ=255) gAAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGt > 1:2819559/1‑68 (MQ=255) gAAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGt > 1:14534/1‑68 (MQ=255) gAAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGt > 1:1421980/1‑68 (MQ=255) gAAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGt > 1:2209153/1‑68 (MQ=255) gAAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGt > 1:3134418/1‑68 (MQ=255) gAAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGt > 1:3372047/1‑68 (MQ=255) gAAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGt > 1:538427/1‑68 (MQ=255) gAAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGt > 1:1153071/1‑68 (MQ=255) gAAGACTTTGCTCGCACGATGCCGGAATATTGAGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGt > 1:3069275/1‑68 (MQ=255) aGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTaa < 1:3076905/69‑1 (MQ=255) aGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTaa < 1:2097588/69‑1 (MQ=255) aGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTaa < 1:2067669/69‑1 (MQ=255) aGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTaa < 1:997718/69‑1 (MQ=255) aGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTaa < 1:1423431/69‑1 (MQ=255) aGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTaa < 1:1178106/69‑1 (MQ=255) gACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGAc < 1:2220979/53‑1 (MQ=255) gACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGAc < 1:63537/53‑1 (MQ=255) gACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGAc < 1:3296063/53‑1 (MQ=255) gACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTaa < 1:2231866/68‑1 (MQ=255) cTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGaa < 1:1245701/67‑1 (MQ=255) gCACGATGCCGGAATATTGCGGCGTGATTTCCAAAAGCCCGACGGTGaa > 1:1334117/1‑49 (MQ=255) gCACGATGCCGGAATATTGCGGCGTGATTTCCAAAAGCCCGACGGTGAAAGCGGTTAAATCGAAGAt > 1:875763/1‑67 (MQ=255) gCACGATGCCGGAATATTGCGGCGTGATTTCCAAAAGCCCGACGGTGAAAGCGGTTAAATCGAAGAt > 1:1968003/1‑67 (MQ=255) gCACGATGCCGGAATATTGCGGCGTGATTTCCAAAAGCCCGACGGTGAAAGCGGTTAAATCGAAGAt > 1:2504950/1‑67 (MQ=255) gCACGATGCCGGAATATTGCGGCGTGATTTCCAAAAGCCCGACGGTGAAAGCGGTTAAATCGAAGAt > 1:1615125/1‑67 (MQ=255) gCACGATGCCGGAATATTGCGGCGTGATTTCCAAAAGCCCGACGGTGAAAGCGGTTAAATCGAAGAt > 1:1607771/1‑67 (MQ=255) ggAATATTGTGGTGTTATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGgaag < 1:1232000/68‑1 (MQ=255) ggAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGgaag < 1:2613056/68‑1 (MQ=255) ggAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGgaag < 1:1872466/68‑1 (MQ=255) ggAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGgaag < 1:3043280/68‑1 (MQ=255) ggAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGgaag < 1:1404595/68‑1 (MQ=255) ggAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGgaag < 1:364317/68‑1 (MQ=255) ggAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGgaag < 1:1654374/68‑1 (MQ=255) ttGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAagaagagaa > 1:3385502/1‑69 (MQ=255) ttGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAagaagagaa > 1:1332081/1‑69 (MQ=255) ttGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAagaagagaa > 1:1218746/1‑69 (MQ=255) ttGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAagaagagaa > 1:756310/1‑69 (MQ=255) ttGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAagaagagaa > 1:303766/1‑69 (MQ=255) ttGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAagaagagaa > 1:7852/1‑69 (MQ=255) ttGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAagaagagaa > 1:1049347/1‑69 (MQ=255) ttGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAagaagagaa > 1:3025362/1‑69 (MQ=255) ttGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAagaagagaa > 1:2976152/1‑69 (MQ=255) ttGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAagaagagaa > 1:1924980/1‑69 (MQ=255) ttGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAagaagagaa > 1:228465/1‑69 (MQ=255) ttGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAagaagagaa > 1:1703369/1‑69 (MQ=255) ttGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAagaagagaa > 1:2139018/1‑69 (MQ=255) ttGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAagaagagaa > 1:2064556/1‑69 (MQ=255) ttGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAagaagaga < 1:2572847/68‑1 (MQ=255) ttGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAagaagaga < 1:1650191/68‑1 (MQ=255) ttGTGGTGTGATCTACAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAagaagagaa > 1:1927161/1‑69 (MQ=255) gtgtGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAAGAAGAGAAGTTCg > 1:736546/1‑69 (MQ=255) gtgtGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAAGAAGAGAAGTTCg > 1:1104579/1‑69 (MQ=255) tgATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGGTTGAAGCGGAAGAAGAGAAGTTCGAc > 1:3200732/1‑68 (MQ=255) tgATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAAGAAGAGAAGTTCGAc > 1:1588151/1‑68 (MQ=255) tgATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAAGAAGAGAAGTTCGAc > 1:1863472/1‑68 (MQ=255) tgATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAAGAAGAGAAGTTCGAc > 1:2640725/1‑68 (MQ=255) aTCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAAGAAGAGAAGTTCg > 1:2213489/1‑64 (MQ=255) aTCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAAGAAGAGAAGTTCg > 1:3193667/1‑64 (MQ=255) ctcCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAagaagaga > 1:465156/1‑56 (MQ=255) ctcCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAagaagaga > 1:1599938/1‑56 (MQ=255) ctcCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAagaagaga > 1:2662407/1‑56 (MQ=255) ctcCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAAGAAGAGAAGTTCGACTTCAg > 1:484520/1‑69 (MQ=255) ctcCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAAGAAGAGAAGTTCGACTTCAg > 1:1998754/1‑69 (MQ=255) ctcCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAAGAAGAGAAGTTCGACTTCAg > 1:79105/1‑69 (MQ=255) ctcCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAAGAAGAGAAGTTCGACTTCAg > 1:929800/1‑69 (MQ=255) ctcCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAAGAA‑AGAAGTTCGACTTCAg < 1:2072295/68‑1 (MQ=255) | ATCAACCTGGCCCGCCAGATTGGCACCGAAGACTTTGCTCGCACGATGCCGGAATATTGTGGTGTGATCTCCAAAAGCCCGACGGTGAAAGCAGTTAAATCGAAGATTGAAGCGGAAGAAGAGAAGTTCGACTTCAG > minE/270990‑271126 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |