| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 271,637 | C→T | 31.6% | P158P (CCG→CCA) | yajL ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 271,637 | 0 | C | T | 31.6% | 33.4 / 17.1 | 41 | P158P (CCG→CCA) | yajL | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (11/17); new base T (9/4); total (20/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTCCAGCCAT > minE/271569‑271697 | aCTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTAccc > 1:2105923/1‑69 (MQ=255) ttCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCgg > 1:2279723/1‑69 (MQ=255) ttCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCgg > 1:1490731/1‑69 (MQ=255) ttCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCgg > 1:3099124/1‑69 (MQ=255) ttCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCgg > 1:274009/1‑69 (MQ=255) ttCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCgg > 1:187890/1‑69 (MQ=255) cATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGc > 1:2283538/1‑68 (MQ=255) ggCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCctg > 1:86976/1‑69 (MQ=255) gCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGCa < 1:1602088/69‑1 (MQ=255) gCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGCa < 1:3067980/69‑1 (MQ=255) gCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGCa < 1:2965995/69‑1 (MQ=255) aaccaacAGGTCTATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGCAAttt < 1:995366/69‑1 (MQ=255) aaccaacAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGCAAttt < 1:1938537/69‑1 (MQ=255) aaccaacAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGCAAttt < 1:1406772/69‑1 (MQ=255) aaccaacAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGCAAttt < 1:2611776/69‑1 (MQ=255) ggTCGATAATTTTCAGACCATAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTg < 1:307461/69‑1 (MQ=255) ggTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTg < 1:839483/69‑1 (MQ=255) ggTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTg < 1:2113016/69‑1 (MQ=255) ggTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTg < 1:2729762/69‑1 (MQ=255) ggTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTg < 1:2395171/69‑1 (MQ=255) ggTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTg < 1:2675016/69‑1 (MQ=255) tttCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTg < 1:2180007/59‑1 (MQ=255) taCCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGCAAttt < 1:1266995/45‑1 (MQ=255) cAAAGTCGATAGCTGTACCTGGCCCCTGGCTGGTCAGCAAtt > 1:2720001/1‑42 (MQ=255) cAAAGTCGATAGCTGTACCTGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCt > 1:1471011/1‑69 (MQ=255) cAAAGTCGATAGCTGTACCTGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCt > 1:1975870/1‑69 (MQ=255) cAAAGTCGATAGCTGTACCTGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCt > 1:2892804/1‑69 (MQ=255) cAAAGTCGATAGCTGTACCTGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCt > 1:1790325/1‑69 (MQ=255) cAAAGTCGATAGCTGTACCTGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCt > 1:1752506/1‑69 (MQ=255) cAAAGTCGATAGCTGTACCTGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCt > 1:3137229/1‑69 (MQ=255) cAAAGTCGATAGCTGTACCTGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCt > 1:3145926/1‑69 (MQ=255) cAAAGTCGATAGCTGTACCTGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCt > 1:3290581/1‑69 (MQ=255) tAGCTGTACCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTCCAGc < 1:1547914/68‑1 (MQ=255) tAGCTGTACCCGGCCCCTGGCTGATCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTCCAGc < 1:258735/68‑1 (MQ=255) gCTGTACCTGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTCCTGCCAt < 1:1789716/69‑1 (MQ=255) gCTGTACCTGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTCCTGCCAt < 1:788678/69‑1 (MQ=255) gCTGTACCTGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTCCTGCCAt < 1:711320/69‑1 (MQ=255) gCTGTACCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTCCAGCCAt > 1:1929537/1‑69 (MQ=255) gCTGTACCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTCCAGCCAt > 1:28467/1‑69 (MQ=255) gCTGTACCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTCCAGCCAt > 1:2230662/1‑69 (MQ=255) gCTGTACCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTCCAGCCAt > 1:1196131/1‑69 (MQ=255) cTGTACCTGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTCCTGCCAt < 1:2627596/68‑1 (MQ=255) | ACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGTCAGCAATTTTACCCGTGCATCCCAGACGACGCGCTTGTCCAGCCAT > minE/271569‑271697 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |