| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 275,944 | C→A | 34.6% | V77V (GTG→GTT) | cyoE ← | protoheme IX farnesyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 275,944 | 0 | C | A | 34.6% | 134.0 / 88.1 | 124 | V77V (GTG→GTT) | cyoE | protoheme IX farnesyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (41/40); new base A (21/22); total (62/62) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01 | |||||||||||
Only 100 of 125 total aligned reads displayed.
AAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAA > minE/275882‑276009 | aaGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGc > 1:723014/1‑69 (MQ=255)aaGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGc > 1:815682/1‑69 (MQ=255)aaGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGc > 1:2453875/1‑69 (MQ=255) cAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGAt < 1:1230236/69‑1 (MQ=255) aCCCAGCAACGTGGCGTACACCAGCTAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGAttct > 1:457939/1‑68 (MQ=255) aCCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGAttctt > 1:3260077/1‑69 (MQ=255) aCCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGAttctt > 1:2076752/1‑69 (MQ=255) aCCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGAttctt > 1:2227579/1‑69 (MQ=255) aCCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGAttctt > 1:1710575/1‑69 (MQ=255) aCCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGAttctt > 1:1606177/1‑69 (MQ=255) aCCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGAttctt > 1:10604/1‑69 (MQ=255) aCCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGAttctt > 1:1140092/1‑69 (MQ=255) aCCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGAttctt > 1:426841/1‑69 (MQ=255) aCCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGAttctt > 1:2995920/1‑69 (MQ=255) aCCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGAttctt > 1:2932444/1‑69 (MQ=255) aCCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGAttct > 1:1551563/1‑68 (MQ=255) aCCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGAttct > 1:2382119/1‑68 (MQ=255) gTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCtt < 1:1330045/69‑1 (MQ=255) acacCATCGAGACAGCAGGAGAGATCAGGCCTTTAACCAGCACCCGATTCTTCGTCCTTTCCATCTTTc > 1:437058/1‑69 (MQ=255) acacCAGCGAGACAGCAGGAGAGATCAGGCCTTTAACCAGCACCCGAttcttc > 1:2553422/1‑53 (MQ=255) acacCAGCGAGACAGCAGGAGAGATCAGGCCTTTAACCAGCACCCGATTCTTCGTCCTTTCCATCTTTc > 1:352597/1‑69 (MQ=255) acacCAGCGAGACAGCAGGAGAGATCAGGCCTTTAACCAGCACCCGATTCTTCGTCCTTTCCATCTTTc > 1:3294605/1‑69 (MQ=255) acacCAGCGAGACAGCAGGAGAGATCAGGCCTTTAACCAGCACCCGATTCTTCGTCCTTTCCATCTTTc > 1:2712374/1‑69 (MQ=255) acacCAGCGAGACAGCAGGAGAGATCAGGCCTTTAACCAGCACCCGATTCTTCGTCCTTTCCATCTTTc > 1:674453/1‑69 (MQ=255) acacCAGCGAGACAGCAGGAGAGATCAGGCCTTTAACCAGCACCCGATTCTTCGTCCTTTCCATCTTTc > 1:3094386/1‑69 (MQ=255) acacCAGCGAGACAGCAGGAGAGATCAGGCCTTTAACCAGCACCCGATTCTTCGTCCTTTCCATCTTTc > 1:2170427/1‑69 (MQ=255) acacCAGCGAGACAGCAGGAGAGATCAGGCCTTTAACCAGCACCCGATTCTTCGTCCTTTCCATCTTTc > 1:968930/1‑69 (MQ=255) acacCAGCGAGACAGCAGGAGAGATCAGGCCTTTAACCAGCACCCGATTCTTCGTCCTTTCCATCTTTc > 1:2033165/1‑69 (MQ=255) acacCAGCGAGACAGCAGGAGAGATCAGGCCTTTAACCAGCACCCGATTCTTCGTCCTTTCCATCTTTc > 1:965975/1‑69 (MQ=255) acacCAGCGAGACAGCAGGAGAGATCAGGCCTTTAACCAGCACCCGATTCTTCGTCCTTTCCATCTTTc > 1:719298/1‑69 (MQ=255) acacCAGCGAGACAGCAGGAGAGATCAGGCCTTTAACCAGCACCCGATTCTTCGTCCTTTCCATCTTTc > 1:1363680/1‑69 (MQ=255) acacCAGCGAGACAGCAGGAGAGATCAGGCCTTTAACCAGCACCCGATTCTTCGTCCTTTCCATCTTTc > 1:1425761/1‑69 (MQ=255) acacCAGCGAGACAGCAGGAGAGATCAGGCCTTTAACCAGCACCCGATTCTTCGTCCTTTCCATCTTTc > 1:148465/1‑69 (MQ=255) acacCAGCGAGACAGCAGGAGAGATCAGGCCTTTAACCAGCACCCGATTCTTCGTCCTTTCCATCTTTc > 1:86345/1‑69 (MQ=255) aGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCtt > 1:1832878/1‑62 (MQ=255) aGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGt > 1:2382650/1‑67 (MQ=255) aGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTc > 1:2989590/1‑68 (MQ=255) aGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTc > 1:1369030/1‑68 (MQ=255) aGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTc > 1:2377120/1‑68 (MQ=255) aGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTc > 1:2444952/1‑68 (MQ=255) aGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTc > 1:1487076/1‑68 (MQ=255) aGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTc > 1:150835/1‑68 (MQ=255) aGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCg < 1:3292048/69‑1 (MQ=255) aGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCAATCTTTCTGTc > 1:2026037/1‑68 (MQ=255) gCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGa < 1:1560890/69‑1 (MQ=255) gCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGa < 1:1985835/69‑1 (MQ=255) gCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGa < 1:1760781/69‑1 (MQ=255) gaCAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATc < 1:2477280/69‑1 (MQ=255) gaCAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATc < 1:2397993/69‑1 (MQ=255) gaCAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATc < 1:902147/69‑1 (MQ=255) aCAGCAGCAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATc < 1:3130947/68‑1 (MQ=255) gcagGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTg > 1:3291737/1‑69 (MQ=255) gcagGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTg > 1:368330/1‑69 (MQ=255) aTCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTa > 1:327293/1‑68 (MQ=255) aTCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAg > 1:3423218/1‑69 (MQ=255) aTCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAg > 1:1224923/1‑69 (MQ=255) aTCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAg > 1:2543191/1‑69 (MQ=255) aTCAGGCCTTTCACCAGCACCCGATTATTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAg > 1:2547395/1‑69 (MQ=255) cAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAgtt > 1:3297887/1‑69 (MQ=255) ccTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTa < 1:926368/69‑1 (MQ=255) ccTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTa < 1:1660363/69‑1 (MQ=255) ccTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTa < 1:2613164/69‑1 (MQ=255) cTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTa < 1:692278/68‑1 (MQ=255) tttCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTaaa < 1:85853/69‑1 (MQ=255) tttCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTaaa < 1:665789/69‑1 (MQ=255) tttCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTaaa < 1:484322/69‑1 (MQ=255) tttCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTaaa < 1:2613318/69‑1 (MQ=255) tttCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTaaa < 1:465849/69‑1 (MQ=255) tttCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTaaa < 1:323430/69‑1 (MQ=255) tttCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTaaa < 1:1079268/69‑1 (MQ=255) tttCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTaaa < 1:1131402/69‑1 (MQ=255) tttCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTaaa < 1:1639901/69‑1 (MQ=255) tttCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTaaa < 1:1730767/69‑1 (MQ=255) tttCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTaaa < 1:1756577/69‑1 (MQ=255) tttCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTaaa < 1:2008850/69‑1 (MQ=255) tttCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTaaa < 1:2553867/69‑1 (MQ=255) tttCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTaaa < 1:262968/69‑1 (MQ=255) tttCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTaaa < 1:3274877/69‑1 (MQ=255) tttCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTaaa < 1:2843204/69‑1 (MQ=255) tttAACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTa < 1:362078/60‑1 (MQ=255) tttAACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTa < 1:3005519/60‑1 (MQ=255) tttAACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTa < 1:957470/60‑1 (MQ=255) tttAACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTa < 1:1311687/60‑1 (MQ=255) tttAACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTa < 1:1421666/60‑1 (MQ=255) tttAACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTa < 1:2976927/60‑1 (MQ=255) tttAACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTa < 1:857579/60‑1 (MQ=255) tttAACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTa < 1:2816539/60‑1 (MQ=255) tttAACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTa < 1:1825350/60‑1 (MQ=255) tttAACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTa < 1:674864/60‑1 (MQ=255) tttAACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTa < 1:2199246/60‑1 (MQ=255) tttAACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTa < 1:222913/60‑1 (MQ=255) tttAACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTa < 1:542280/60‑1 (MQ=255) tttAACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTa < 1:24807/60‑1 (MQ=255) tttAACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTa < 1:2600148/60‑1 (MQ=255) tttAACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTa < 1:408730/60‑1 (MQ=255) tttAACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTa < 1:427114/60‑1 (MQ=255) ttCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTaaa < 1:183716/68‑1 (MQ=255) ttCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTaaa < 1:613302/68‑1 (MQ=255) tCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCg < 1:2803604/41‑1 (MQ=255) cACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATc < 1:2728203/45‑1 (MQ=255) | AAGCCAGCAATACCCAGCAACGTGGCGTACACCAGCGAGACAGCAGGAGAGATCAGGCCTTTCACCAGCACCCGATTCTTCGTCCTTTCCATCTTTCTGTCGATATCCCTGTCGATGTAGTTGTTAAA > minE/275882‑276009 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |