| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,247 | T→A | 32.2% | S637S (TCT→TCA) | thrA → | fused aspartokinase I and homoserine dehydrogenase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,247 | 0 | T | A | 32.2% | 63.8 / 43.3 | 62 | S637S (TCT→TCA) | thrA | fused aspartokinase I and homoserine dehydrogenase I |
| Reads supporting (aligned to +/- strand): ref base T (10/32); new base A (3/17); total (13/49) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.20e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.82e-01 | |||||||||||
GAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACGCT > minE/2185‑2312 | gagaACCTGCAAAATCTGCTCAATGCAGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCTGGTTCg > 1:2032932/1‑69 (MQ=255)gagaACCTGCAAAATCTGCTCAATGCAGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCTGGTTCg > 1:2773219/1‑69 (MQ=255) aCCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCttt < 1:1690082/69‑1 (MQ=255) aCCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCttt < 1:2496709/69‑1 (MQ=255) aCCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCttt < 1:1456974/69‑1 (MQ=255) aCCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCttt < 1:1482304/69‑1 (MQ=255) aCCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCttt < 1:240329/69‑1 (MQ=255) aCCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCttt < 1:2679190/69‑1 (MQ=255) aCCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCttt < 1:501088/69‑1 (MQ=255) aCCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCttt < 1:2832265/69‑1 (MQ=255) aCCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCttt < 1:2290945/69‑1 (MQ=255) aCCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCttt < 1:1637966/69‑1 (MQ=255) aCCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCttt < 1:412499/69‑1 (MQ=255) aCCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCttt < 1:1042771/69‑1 (MQ=255) ccTGCAAAATCTGCTCAATGCAGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCTGGTTCGCttt > 1:2811814/1‑68 (MQ=255) ccTGCAAAATCTGCTCAATGCAGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCTGGTTCGCttt > 1:412942/1‑68 (MQ=255) cTGCAAAATCTGCTCAATGCAGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCTGGTTCGCTTTCt < 1:2446871/69‑1 (MQ=255) cTGCAAAATCTGCTCAATGCAGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCTGGTTCGCTTTCt < 1:2598539/69‑1 (MQ=255) tGCAGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCTGGTTCGCTTTCTTATATCTTCGGCAAGt < 1:2262425/68‑1 (MQ=255) gCAGGTGATGAATTGATGAAGTTCTCCGTCATTCTTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTa < 1:3058158/69‑1 (MQ=255) gCAGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTa < 1:1702408/69‑1 (MQ=255) gCAGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTa < 1:2055293/69‑1 (MQ=255) gCAGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTa < 1:1284694/69‑1 (MQ=255) gCAGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTa < 1:1255670/69‑1 (MQ=255) gCAGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTa < 1:2286734/69‑1 (MQ=255) gCAGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTa < 1:1063607/69‑1 (MQ=255) gCAGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTa < 1:2059303/69‑1 (MQ=255) gatgaATTGATGAAGTTCTCCGGCATTCTTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGaa > 1:2080863/1‑69 (MQ=255) gaATTGATGAAGTTCTCCGGCATTCTTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAgg < 1:2013351/68‑1 (MQ=255) gaATTGATGAAGTTCTCCGGCATTCTTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAgg < 1:1858158/68‑1 (MQ=255) gaATTGATGAAGTTCTCCGGCATTCTTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAgg < 1:2091881/68‑1 (MQ=255) gaATTGATGAAGTTCTCCGGCATTCTTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAgg < 1:1562029/68‑1 (MQ=255) gaATTGATGAAGTTCTCCGGCATTCTTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAgg < 1:2659940/68‑1 (MQ=255) gaATTGATGAAGTTCTCCGGCATTCTTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAgg < 1:2989196/68‑1 (MQ=255) gaATTGATGAAGTTCTCCGGCATTCTTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAgg < 1:3343197/68‑1 (MQ=255) gaATTGATGAAGTTCTCCGGCATTCTTTCTGGTTCGCTTTCTCATATCTTCGGCAAGTTAGACGAAgg < 1:586148/68‑1 (MQ=255) gaATTGATGAAGTTCTCCGGCATTCTTTCTGGTTCGCTTTCTCATATCTTCGGCAAGTTAGACGAAgg < 1:590453/68‑1 (MQ=255) gaATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGc < 1:736109/69‑1 (MQ=255) gaATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGc < 1:3188161/69‑1 (MQ=255) gaATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGc < 1:1096211/69‑1 (MQ=255) gaATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGc < 1:1667338/69‑1 (MQ=255) aaTTGATGAAGTTCTCCGGCATTCTTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAgg < 1:3044812/67‑1 (MQ=255) ttGATGAAGTTCTCCGGCATTCTTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACg < 1:3071360/61‑1 (MQ=255) ttGATGAAGTTCTCCGGCATTCTTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACg < 1:808126/61‑1 (MQ=255) ttGATGAAGTTCTCCGGCATTCTTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACg < 1:1363753/61‑1 (MQ=255) ttGATGAAGTTCTCCGGCATTCTTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACg < 1:1368229/61‑1 (MQ=255) ttGATGAAGTTCTCCGGCATTCTTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACg < 1:2552125/61‑1 (MQ=255) ttGATGAAGTTCTCCGGCATTCTTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACg < 1:2235754/61‑1 (MQ=255) ttGATGAAGTTCTCCGGCATTCTTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACg < 1:756540/61‑1 (MQ=255) ttGATGAAGTTCTCCGGCATTCTTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACg < 1:1051219/61‑1 (MQ=255) ttGATGAAGTTCTCCGGCATTCTTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACg < 1:1940671/61‑1 (MQ=255) tgatgaAGTTCTCCGGCATTCTTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAgg < 1:2174529/64‑1 (MQ=255) tgatgaAGTTCTCCGGCATTCTTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGa > 1:1600111/1‑69 (MQ=255) tgaAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGc < 1:75923/62‑1 (MQ=255) gaAGTTCTCCGGCATTCTTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGttt > 1:2105109/1‑69 (MQ=255) gaAGTTCTCCGGCATTCTTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGttt > 1:319780/1‑69 (MQ=255) gaAGTTCTCCGGCATTCTTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGttt > 1:1462296/1‑69 (MQ=255) ctcCGGCATTCTTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGa > 1:310385/1‑69 (MQ=255) tcCGACATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACg > 1:1938184/1‑49 (MQ=38) tcCGACATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGa > 1:3115963/1‑50 (MQ=39) tcCGACATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGa > 1:924245/1‑50 (MQ=39) ggCATTCTTTCTTGTTCGCTTTCTTATATCTTCGGCAAGTTa < 1:1600797/42‑1 (MQ=255) ttctttctGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCa < 1:892198/69‑1 (MQ=255) ttctttctGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCa < 1:1587165/69‑1 (MQ=255) ttctttctGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCa < 1:1006653/69‑1 (MQ=255) ttctttctGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCa < 1:160987/69‑1 (MQ=255) ttctttctGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCa < 1:1916099/69‑1 (MQ=255) tctttctGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCAc < 1:1249233/69‑1 (MQ=255) tctttctGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCAc < 1:1121264/69‑1 (MQ=255) tctttctGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCAc < 1:2842823/69‑1 (MQ=255) tctttctGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCAc < 1:2265596/69‑1 (MQ=255) tctttctGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCAc < 1:2139047/69‑1 (MQ=255) tctttctGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCAc < 1:107676/69‑1 (MQ=255) tctttctGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCAc < 1:1729126/69‑1 (MQ=255) tctttctGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCAc < 1:1860132/69‑1 (MQ=255) ctttctGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACg > 1:426810/1‑69 (MQ=255) ctttctGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACg > 1:782212/1‑69 (MQ=255) ctttctGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACg > 1:51395/1‑69 (MQ=255) ctttctGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACg > 1:740060/1‑69 (MQ=255) ctttctGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACg > 1:988001/1‑69 (MQ=255) ctttctGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACg > 1:1821418/1‑69 (MQ=255) ctttctGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACg > 1:1015225/1‑69 (MQ=255) ctttctGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACg > 1:1102572/1‑69 (MQ=255) ctttctGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACg > 1:1209758/1‑69 (MQ=255) ctttctGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACg > 1:1616357/1‑69 (MQ=255) ctttctGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACg > 1:1671633/1‑69 (MQ=255) ctttctGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACg > 1:3374133/1‑69 (MQ=255) ctttctGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACg > 1:1895360/1‑69 (MQ=255) ctttctGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACg > 1:1976042/1‑69 (MQ=255) ctttctGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACg > 1:2732983/1‑69 (MQ=255) tGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACGCt < 1:555341/66‑1 (MQ=255) tGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACGCt < 1:1690574/66‑1 (MQ=255) tGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACGCt < 1:2180050/66‑1 (MQ=255) tGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACGCt < 1:1627679/66‑1 (MQ=255) tGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACGCt < 1:2394940/66‑1 (MQ=255) tGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACGCt < 1:1267405/66‑1 (MQ=255) tGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACGCt < 1:3152492/66‑1 (MQ=255) tGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACGCt < 1:3291847/66‑1 (MQ=255) | GAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACGCT > minE/2185‑2312 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |