| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 276,399 | T→C | 53.4% | G39G (GGA→GGG) | cyoD ← | cytochrome o ubiquinol oxidase subunit IV |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 276,399 | 0 | T | C | 53.4% | 8.8 / 62.3 | 75 | G39G (GGA→GGG) | cyoD | cytochrome o ubiquinol oxidase subunit IV |
| Reads supporting (aligned to +/- strand): ref base T (18/17); new base C (30/10); total (48/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.31e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCACCAGAACCTGTACCACTGCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCCTGTCATCACCATCCAGAACGGAATCACCGTCAGAATGATCGACAGGATAAAGCCTGTCA > minE/276335‑276460 | gCACCAGAACCTGTACCACTGCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCCt > 1:2734635/1‑68 (MQ=255)gCACCAGAACCTGTACCACTGCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCCt > 1:2655000/1‑68 (MQ=255)gCACCAGAACCTGTACCACTGCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCCt > 1:3208602/1‑68 (MQ=255) ccAGAACCTGTACCACTGCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCCTGtc > 1:1808277/1‑68 (MQ=255) ccAGAACCTGTACCACTGCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCCTGtc > 1:1636406/1‑68 (MQ=255) ccAGAACCTGTACCACTGCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCCTGtc > 1:86200/1‑68 (MQ=255) tcccATTGCCAGGATTGTTCCCAGAATTACGTCCGGAGAGGCAGCTCCTGTCATCACCATCCAGAACgg < 1:1120567/67‑1 (MQ=255) tGCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCCTGTCATCACCATCCATAACgg < 1:2350410/69‑1 (MQ=255) tGCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCCTGTCATCACCATCCAGAACgg < 1:70532/69‑1 (MQ=255) tGCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCCTGTCATCACCATCCAGAACgg < 1:541959/69‑1 (MQ=255) tGCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCCTGTCATCACCATCCAGAACgg < 1:3127611/69‑1 (MQ=255) tGCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCCTGTCATCACCATCCAGAACgg < 1:2382931/69‑1 (MQ=255) tGCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCCTGTCATCACCATCCAGAACgg < 1:909637/69‑1 (MQ=255) gCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCCTGtc > 1:1338941/1‑51 (MQ=255) gCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCCTGtc > 1:3328155/1‑51 (MQ=255) gCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCCTGtc > 1:1583307/1‑51 (MQ=255) gCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCCTGtc > 1:867325/1‑51 (MQ=255) gCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCCTGt > 1:1022151/1‑50 (MQ=255) gCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCCTGTCATCACCATCCAGAACGGa < 1:2382829/69‑1 (MQ=255) gCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCCTGTCATCACCATCCAGAACGGa < 1:903774/69‑1 (MQ=255) gCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCCTGTCATCACCATCCAGAACGGa < 1:1840326/69‑1 (MQ=255) gCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCCTGTCATCACCATCCAGAACGGa < 1:126686/69‑1 (MQ=255) gCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCCTGTCATCACCATCCAGAACGGa < 1:1621119/69‑1 (MQ=255) gCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCCTGTCATCACCATCCAGAACGGa < 1:626487/69‑1 (MQ=255) gCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCCTGTCATCACCATCCAGAACGGa < 1:2708635/69‑1 (MQ=255) ccATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCCTGTCATCACCATCCAGAACGGa < 1:27233/68‑1 (MQ=255) gCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCCTGtc > 1:1661302/1‑45 (MQ=255) gCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCCTGTCATCACCATCCAGAACgg > 1:2575906/1‑62 (MQ=255) gCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCCTGTCATCACCATCCAGAACGGAATCAcc > 1:3419621/1‑69 (MQ=255) gCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCCTGTCATCACCATCCAGAACGGAATCAcc > 1:1089207/1‑69 (MQ=255) gCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCCTGTCATCACCATCCAGAACGGAATCAcc > 1:2636070/1‑69 (MQ=255) gCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCCTGTCATCACCATCCAGAACGGAATCAcc > 1:195024/1‑69 (MQ=255) gCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCCTGTCATCACCATCCAGAACGGAATCAcc > 1:1823938/1‑69 (MQ=255) cAGAATTACGGCCGGAGAGGCAGCTCCTGTCATCACCATCCAGAACGGAATCACCGTCAGAATGATCg < 1:816387/68‑1 (MQ=255) ccGGAGAGGCAGCCCCTGTCATCACCATCCAGAACGGAATCa > 1:218287/1‑42 (MQ=255) ccGGAGAGGCAGCCCCTGTCATCACCATCCAGAACGGAATCACCGt > 1:1368793/1‑46 (MQ=255) ccGGAGAGGCAGCCCCTGTCATCACCATCCAGAACGGAATCACCGTCAGAATGATCGACAgg > 1:96836/1‑62 (MQ=255) ccGGAGAGGCAGCCCCTGTCATCACCATCCAGAACGGAATCACCGTCAGAATGATCGACAgg > 1:2855937/1‑62 (MQ=255) ccGGAGAGGCAGCCCCTGTCATCACCATCCAGAACGGAATCACCGTCAGAATGATCGACAgg > 1:3147481/1‑62 (MQ=255) ccGGAGAGGCAGCCCCTGTCATCACCATCCAGAACGGAATCACCGTCAGAATGATCGACAgg > 1:854943/1‑62 (MQ=255) ccGGAGAGGCAGCCCCTGTCATCACCATCCAGAACGGAATCACCGTCAGAATGATCGACAgg > 1:3389861/1‑62 (MQ=255) ccGGAGAGGCAGCCCCTGTCATCACCATCCAGAACGGAATCACCGTCAGAATGATCGACAgg > 1:453422/1‑62 (MQ=255) ccGGAGAGGCAGCCCCTGTCATCACCATCCAGAACGGAATCACCGTCAGAATGATCGACAgg > 1:465164/1‑62 (MQ=255) ccGGAGAGGCAGCCCCTGTCATCACCATCCAGAACGGAATCACCGTCAGAATGATCGACAgg > 1:525227/1‑62 (MQ=255) ccGGAGAGGCAGCCCCTGTCATCACCATCCAGAACGGAATCACCGTCAGAATGATCGACAgg > 1:1682272/1‑62 (MQ=255) ccGGAGAGGCAGCCCCTGTCATCACCATCCAGAACGGAATCACCGTCAGAATGATCGACAgg > 1:2013220/1‑62 (MQ=255) ccGGAGAGGCAGCCCCTGTCATCACCATCCAGAACGGAATCACCGTCAGAATGATCGACAgg > 1:2747997/1‑62 (MQ=255) ccGGAGAGGCAGCCCCTGTCATCACCATCCAGAACGGAATCACCGTCAGAATGATCGACAgg > 1:2124505/1‑62 (MQ=255) ccGGAGAGGCAGCCCCTGTCATCACCATCCAGAACGGAATCACCGTCAGAATGATCGACAgg > 1:1766632/1‑62 (MQ=255) ccGGAGAGGCAGCCCCTGTCATCACCATCCAGAACGGAATCACCGTCAGAATGATCGACAgg > 1:2201190/1‑62 (MQ=255) ccGGAGAGGCAGCCCCTGTCATCACCATCCAGAACGGAATCACCGTCAGAATGATCGACAgg > 1:226480/1‑62 (MQ=255) ccGGAGAGGCAGCCCCTGTCATCACCATCCAGAACGGAATCACCGTCAGAATGATCGACAgg > 1:1435717/1‑62 (MQ=255) ccGGAGAGGCAGCCCCTGTCATCACCATCCAGAACGGAATCACCGTCAGAATGATCGACAgg > 1:2340572/1‑62 (MQ=255) ccGGAGAGGCAGCCCCTGTCATCACCATCCAGAACGGAATCACCGTCAGAATGATCGACAgg > 1:1139794/1‑62 (MQ=255) ccGGAGAGGCAGCCCCTGTCATCACCATCCAGAACGGAATCACCGTCAGAATGATCGACAgg > 1:1087054/1‑62 (MQ=255) ccGGAGAGGCAGCCCCTGTCATCACCATCCAGAACGGAATCACCGTCAGAATGATAGACAg > 1:3065345/1‑61 (MQ=255) cGGAGAGGCAGCCCCTGTCATCACCATCCAGAACGGAATCACCGTCAGAATGATCGACAGGATAAAGcc > 1:434664/1‑69 (MQ=255) cGGAGAGGCAGCCCCTGTCATCACCATCCAGAACGGAATCACCGTCAGAATGATCGACAGGATAAAGcc > 1:1278651/1‑69 (MQ=255) cGGAGAGGCAGCCCCTGTCATCACCATCCAGAACGGAATCACCGTCAGAATGATCGACAGGATAAAGcc > 1:1227188/1‑69 (MQ=255) cGGAGAGGCAGCCCCTGTCATCACCATCCAGAACGGAATCACCGTCAGAATGATCGACAGGATAAAGcc > 1:55077/1‑69 (MQ=255) cGGAGAGGCAGCCCCTGTCATCACCATCCAGAACGGAATCACCGTCAGAATGATCGACAGGATAAAGcc > 1:1981522/1‑69 (MQ=255) cGGAGAGGCAGCCCCTGTCATCACCATCCAGAACGGAATCACCGTCAGAATGATCGACAGGATAAAGcc > 1:419309/1‑69 (MQ=255) cGGAGAGGCAGCCCCTGTCATCACCATCCAGAACGGAATCACCGTCAGAATGATCGACAGGATAAAGcc > 1:411417/1‑69 (MQ=255) cGGAGAGGCAGCCCCTGTCATCACCATCCAGAACGGAATCACCGTCAGAATGATCGACAGGATAAAGc > 1:836827/1‑68 (MQ=255) agagGCAGCTCCTGTCATCACCATCCAGAACGGAATCACCGTCAGAATGATCGACAGGATAAAGCCTGt < 1:2016061/69‑1 (MQ=255) agGCAGCCCCTGTCATCACCATCCAGAACGGAATCACCGTCAGAATGATCGACAGGATAAAGCCTGTCa < 1:2101951/69‑1 (MQ=255) agGCAGCCCCTGTCATCACCATCCAGAACGGAATCACCGTCAGAATGATCGACAGGATAAAGCCTGTCa < 1:3404555/69‑1 (MQ=255) agGCAGCCCCTGTCATCACCATCCAGAACGGAATCACCGTCAGAATGATCGACAGGATAAAGCCTGTCa < 1:2318978/69‑1 (MQ=255) agGCAGCCCCTGTCATCACCATCCAGAACGGAATCACCGTCAGAATGATCGACAGGATAAAGCCTGTCa < 1:152300/69‑1 (MQ=255) agGCAGCCCCTGTCATCACCATCCAGAACGGAATCACCGTCAGAATGATCGACAGGATAAAGCCTGTCa < 1:2322743/69‑1 (MQ=255) agGCAGCCCCTGTCATCACCATCCAGAACGGAATCACCGTCAGAATGATCGACAGGATAAAGCCTGTCa < 1:1320383/69‑1 (MQ=255) agGCAGCCCCTGTCATCACCATCCAGAACGGAATCACCGTCAGAATGATCGACAGGATAAAGCCTGTCa < 1:3087224/69‑1 (MQ=255) agGCAGCCCCTGTCATCACCATCCAGAACGGAATCACCGTCAGAATGATCGACAGGATAAAGCCTGTCa < 1:2954455/69‑1 (MQ=255) agGCAGCCCCTGTCATCACCATCCAGAACGGAATCACCGTCAGAATGATCGACAGGATAAAGCCTGTCa < 1:2360527/69‑1 (MQ=255) agGCAGCCCCTGTCATCACCATCCAGAACGGAATCACCGTCAGAATGATCGACAGGATAAAGCCTGTCa < 1:2780193/69‑1 (MQ=255) | GCACCAGAACCTGTACCACTGCCATTGCCAGGATTGTTCCCAGAATTACGGCCGGAGAGGCAGCTCCTGTCATCACCATCCAGAACGGAATCACCGTCAGAATGATCGACAGGATAAAGCCTGTCA > minE/276335‑276460 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |