| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 277,691 | G→A | 48.9% | L474L (CTG→TTG) | cyoB ← | cytochrome o ubiquinol oxidase subunit I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 277,691 | 0 | G | A | 48.9% | ‑1.6 / 38.7 | 47 | L474L (CTG→TTG) | cyoB | cytochrome o ubiquinol oxidase subunit I |
| Reads supporting (aligned to +/- strand): ref base G (8/16); new base A (15/8); total (23/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.22e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGCATGGTGTGGAACTGCGGGTCAATCTGCTGGCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGG > minE/277627‑277759 | aGCATGGTGTGGAACTGCGGGTCAATCTGCTGGCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCa < 1:2300983/69‑1 (MQ=255)aGCATGGTGTGGAACTGCGGGTCAATCTGCTGGCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCa < 1:2871204/69‑1 (MQ=255)aGCATGGTGTGGAACTGCGGGTCAATCTGCTGGCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCa < 1:1565980/69‑1 (MQ=255)aGCATGGTGTGGAACTGCGGGTCAATCTGCTGGCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCa < 1:349768/69‑1 (MQ=255)aGCATGGTGTGGAACTGCGGGTCAATCTGCTGGCTCAAACGACGGGTCATGCACATGAAGCCCAGCGCa < 1:372200/69‑1 (MQ=255) aTGGTGTGGAACTGCGGGTCAATCTGCTGGCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATa > 1:2104919/1‑68 (MQ=255) aTGGTGTGGAACTGCGGGTCAATCTGCTGGCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATAc > 1:1229413/1‑69 (MQ=255) aTGGTGTGGAACTGCGGGTCAATCTGCTGGCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATAc > 1:1230212/1‑69 (MQ=255) gAACTGCGGGTCAATCTGCTGGCTCAAACGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCa > 1:2457894/1‑68 (MQ=255) gAACTGCGGGTCAATCTGCTGGCTCAAACGACGGGTCATCCCCATAAAGCCCAACGCATACAGCGGCa > 1:294297/1‑68 (MQ=255) gAACTGCGGGTCAATCTGCTGGCTCAAACGACGGGACATCCCCATAAAGCCCAACGCATACAGCGGCa > 1:2228543/1‑68 (MQ=255) aCTGCGGGTCAATCTGCTGGCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATaa > 1:1584434/1‑69 (MQ=255) aCTGCGGGTCAATCTGCTGGCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATaa > 1:2755710/1‑69 (MQ=255) aCTGCGGGTCAATCTGCTGGCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATaa > 1:839857/1‑69 (MQ=255) tgctgGCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACgaagaa > 1:1504726/1‑68 (MQ=255) ggCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGcc < 1:1347073/67‑1 (MQ=255) ggCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGcc < 1:1785909/67‑1 (MQ=255) ggCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGcc < 1:3232909/67‑1 (MQ=255) ggCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGcc < 1:601612/67‑1 (MQ=255) gacgGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACgaagaa > 1:3075000/1‑55 (MQ=255) gacgGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACgaagaa > 1:2479776/1‑55 (MQ=255) gacgGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACgaagaa > 1:2414349/1‑55 (MQ=255) gacgGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACgaagaa > 1:288183/1‑55 (MQ=255) gacgGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACgaagaa > 1:3124969/1‑55 (MQ=255) gacgGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACgaagaa > 1:3184512/1‑55 (MQ=255) gacgGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACgaagaa > 1:1920311/1‑55 (MQ=255) gacgGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACgaagaa > 1:1883551/1‑55 (MQ=255) gacgGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACgaagaa > 1:1581516/1‑55 (MQ=255) gacgGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACgaagaa > 1:502257/1‑55 (MQ=255) gacgGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACgaagaa > 1:1238561/1‑55 (MQ=255) gacgGGTCATCCCCATAAAGCCCAACGCATACAGCGGCATAAAGGCAACgaaga > 1:3270767/1‑54 (MQ=255) gTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATccagaaccag < 1:912561/69‑1 (MQ=255) gTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATccagaaccag < 1:2495186/69‑1 (MQ=255) cATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAAcgcgcg < 1:1970500/69‑1 (MQ=255) cATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAAcgcgcg < 1:1316688/69‑1 (MQ=255) cATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAAcgcgcg < 1:1411905/69‑1 (MQ=255) ataaaGCCCAACGCATACAGCTGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGt < 1:797998/66‑1 (MQ=255) ataaaGCCCAACGCATACAGCGGCATAACGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGt < 1:2600628/66‑1 (MQ=255) ataaaGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGt < 1:1529480/66‑1 (MQ=255) ataaaGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGt < 1:1142164/66‑1 (MQ=255) ataaaGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGt < 1:70002/66‑1 (MQ=255) ataaaGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGt < 1:3290157/66‑1 (MQ=255) ataaaGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGt < 1:172430/66‑1 (MQ=255) ataaaGCCCAACGCATACAGCGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGt < 1:1886031/66‑1 (MQ=255) aTGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAAcgcgcg < 1:3425754/68‑1 (MQ=255) tGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGtt < 1:124443/69‑1 (MQ=255) aaGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTa > 1:1017869/1‑69 (MQ=255) gcgcATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAgg < 1:3346134/69‑1 (MQ=255) gcgcATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAgg < 1:2145948/69‑1 (MQ=255) gcgcATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAgg < 1:884966/69‑1 (MQ=255) | AGCATGGTGTGGAACTGCGGGTCAATCTGCTGGCTCAAACGACGGGTCATGCCCATGAAGCCCAGCGCATACAGTGGCATAAAGGCAACGAAGAAGCCGATGATCCAGAACCAGAACGCGCGTTTACCCCAGG > minE/277627‑277759 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |