| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 277,857 | C→T | 50.0% | A418A (GCG→GCA) | cyoB ← | cytochrome o ubiquinol oxidase subunit I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 277,857 | 0 | C | T | 50.0% | ‑3.4 / 64.7 | 72 | A418A (GCG→GCA) | cyoB | cytochrome o ubiquinol oxidase subunit I |
| Reads supporting (aligned to +/- strand): ref base C (23/13); new base T (18/18); total (41/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.41e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01 | |||||||||||
AGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCA > minE/277799‑277924 | aGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGTGCAATCAGGa < 1:45656/69‑1 (MQ=255)aGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGTGCAATCAGGa < 1:1562227/69‑1 (MQ=255)aGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGTGCAATCAGGa < 1:1611324/69‑1 (MQ=255)aGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGTGCAATCAGGa < 1:2121952/69‑1 (MQ=255)aGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGTGCAATCAGGa < 1:2489311/69‑1 (MQ=255)aGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGTGCAATCAGGa < 1:1486266/69‑1 (MQ=255)aGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGTGCAATCAGGa < 1:3102999/69‑1 (MQ=255)aGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGTGCAATCAGGa < 1:475348/69‑1 (MQ=255)aGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGTGCAATCAGGa < 1:708903/69‑1 (MQ=255)aGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGTGCAATCAGGa < 1:831914/69‑1 (MQ=255)aGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGTGCAATCAGGa < 1:1087168/69‑1 (MQ=255)aGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGTGCAATCAGGa < 1:962801/69‑1 (MQ=255) ggTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAgg > 1:1059342/1‑67 (MQ=255) ggTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAgg > 1:812902/1‑67 (MQ=255) ggTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAgg > 1:2547693/1‑67 (MQ=255) ggTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAgg > 1:813074/1‑67 (MQ=255) ggTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAgg > 1:1878886/1‑67 (MQ=255) ggTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAgg > 1:1371483/1‑67 (MQ=255) ggTCATCCCTGCGAAGCAGCCGAAGACCACGACGCCGATGATCACGTTATGGAAGTGTGCAATCAGGa < 1:2538575/68‑1 (MQ=255) gTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGTGCAATCAGGa < 1:1551257/67‑1 (MQ=255) gTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGTGCAATCAGGa < 1:1204541/67‑1 (MQ=255) cATCCATGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAg > 1:1807732/1‑69 (MQ=255) ccTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCt > 1:3260174/1‑68 (MQ=255) ccTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCt > 1:2148004/1‑68 (MQ=255) tGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGt < 1:281594/68‑1 (MQ=255) tGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGt < 1:265764/68‑1 (MQ=255) tGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGt < 1:1112483/68‑1 (MQ=255) tGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGt < 1:574522/68‑1 (MQ=255) gcagcCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAg < 1:1596178/69‑1 (MQ=255) gcagcCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAg < 1:155202/69‑1 (MQ=255) gcagcCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAg < 1:1861836/69‑1 (MQ=255) cGAAGACCACGCCGCCGATGATCACGTTATTGAAGTGTGCAATCAGGa < 1:415804/48‑1 (MQ=255) gAAGACCACGCCGCCGATGATCACGTTATGGAAGTGTGCAATCAGGa < 1:2826236/47‑1 (MQ=255) aGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGt < 1:572929/69‑1 (MQ=255) aGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGt < 1:481044/69‑1 (MQ=255) aGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGt < 1:477085/69‑1 (MQ=255) aGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGt < 1:1722204/69‑1 (MQ=255) aGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGt < 1:359242/69‑1 (MQ=255) gccgATGATCACGTTATGGAAGTGTGCAATCAGGa < 1:1837810/35‑1 (MQ=255) gccgATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCgg < 1:3323640/69‑1 (MQ=255) atgatCACGTTATGGAAGTGTGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCTGCGCCAGGTAct > 1:3106964/1‑68 (MQ=255) atgatCACGTTATGGAAGTGTGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCTGCGCCAGGTAct > 1:583993/1‑68 (MQ=255) atgatCACGTTATGGAAGTGTGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCTGCGCCAGGTAct > 1:848715/1‑68 (MQ=255) atgatCACGTTATGGAAGTGTGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCTGCGCCAGGTAct > 1:3401228/1‑68 (MQ=255) atgatCACGTTATGGAAGTGTGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCTGCGCCAGGTAct > 1:366559/1‑68 (MQ=255) atgatCACGTTATGGAAGTGTGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCTGCGCCAGGTAct > 1:391034/1‑68 (MQ=255) atgatCACGTTATGGAAGTGTGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCTGCGCCAGGTAct > 1:1095651/1‑68 (MQ=255) atgatCACGTTATGGAAGTGTGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCTGCGCCAGGTAct > 1:472939/1‑68 (MQ=255) atgatCACGTTATGGAAGTGTGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCTGCGCCAGGTAct > 1:3258190/1‑68 (MQ=255) atgatCACGTTATGGAAGTGTGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCTGCGCCAGGTAct > 1:2335646/1‑68 (MQ=255) atgatCACGTTATGGAAGTGTGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCTGCGCCAGGTAct > 1:2346713/1‑68 (MQ=255) atgatCACGTTATGGAAGTGTGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCTGCGCCAGGTAct > 1:1529577/1‑68 (MQ=255) atgatCACGTTATGGAAGTGTGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCTGCGCCAGGTAct > 1:2189749/1‑68 (MQ=255) atgatCACGTTATGGAAGTGTGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCTGCGCCAGGTAct > 1:153421/1‑68 (MQ=255) atgatCACGTTATGGAAGTGTGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCTGCGCCAGGTAct > 1:2626286/1‑68 (MQ=255) atgatCACGTTATGGAAGTGTGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCTGCGCCAGGTAct > 1:2682345/1‑68 (MQ=255) atgatCACGTTATGGAAGTGTGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCTGCGCCAGGTAct > 1:3214500/1‑68 (MQ=255) atgatCACGTTATGGAAGTGTGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCTGCGCCAGGTAc > 1:1542650/1‑68 (MQ=255) tGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCTGTACGGCcagca > 1:2262033/1‑64 (MQ=255) tGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCcagcag > 1:2090756/1‑65 (MQ=255) tGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCcagcag > 1:1129242/1‑65 (MQ=255) tGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCcagcag > 1:638641/1‑65 (MQ=255) tGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCcagcag > 1:1655294/1‑65 (MQ=255) tGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCcagcag > 1:943973/1‑65 (MQ=255) tGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCcagcag > 1:1021026/1‑65 (MQ=255) tGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCcagcag > 1:1309900/1‑65 (MQ=255) tGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCcagcag > 1:251656/1‑65 (MQ=255) tGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCcagcag > 1:1499833/1‑65 (MQ=255) tGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCcagcag > 1:291489/1‑65 (MQ=255) tGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCcagcag > 1:2970617/1‑65 (MQ=255) tGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCcagcag > 1:1380933/1‑65 (MQ=255) gTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGt > 1:456715/1‑69 (MQ=255) gcgcAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCa < 1:335552/69‑1 (MQ=255) gcgcAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCa < 1:327100/69‑1 (MQ=255) | AGGTCATCCCTGCGAAGCAGCCGAAGACCACGCCGCCGATGATCACGTTATGGAAGTGCGCAATCAGGAACAGGCTGTTATGCAGAACGAAGTCCGCGCCCGGTACGGCCAGCAGCACGCCAGTCA > minE/277799‑277924 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |