| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 278,199 | G→A | 43.8% | T304T (ACC→ACT) | cyoB ← | cytochrome o ubiquinol oxidase subunit I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 278,199 | 0 | G | A | 43.8% | 24.6 / 68.8 | 89 | T304T (ACC→ACT) | cyoB | cytochrome o ubiquinol oxidase subunit I |
| Reads supporting (aligned to +/- strand): ref base G (32/18); new base A (10/29); total (42/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.40e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 | |||||||||||
GACAGCACGGTGATACAGACGGTTGCCCATACCAGCGAGGTATAACCAAACAGACGTTTACGCGAGAAGGTTGCCGCAATTTCGGAGAACACACCGAAAACAGGCAGGATCAGGATGTAAACTTCCGGGT > minE/278131‑278260 | gACAGCACGGTGATACAGACGGTTGCCCATACCAGCGAGGTATAACCAAACAGACGTTTACGCGAGAAg < 1:3018271/69‑1 (MQ=255)gACAGCACGGTGATACAGACGGTTGCCCATACCAGCGAGGTATAACCAAACAGACGTTTACGCGAGAAg < 1:865883/69‑1 (MQ=255) aCAGCACGGTGATACAGACGGTTGCCCATACCAGCGAGGTATAACCAAACAGACGTTTACGCGAGAAg < 1:2099207/68‑1 (MQ=255) gATACAGACGGTTGCCCATACCAGCGAGGTATAACCAAACAGACGTTTACGCGAGAAGGTTGCCGCaa > 1:1274050/1‑68 (MQ=255) gATACAGACGGTTGCCCATACCAGCGAGGTATAACCAAACAGACGTTTACGCGAGAAGGTTGCCGCAAt > 1:2754205/1‑69 (MQ=255) gATACAGACGGTTGCCCATACCAGCGAGGTATAACCAAACAGACGTTTACGCGAGAAGGTTGCCGCAAt > 1:272519/1‑69 (MQ=255) aCAGACGGTTGCCCATACCAGCGAGGTATAACCAAACAGACGTTTACGCGAGAAGGTTGCCGCAAttt > 1:523123/1‑68 (MQ=255) aCAGACGGTTGCCCATACCAGCGAGGTATAACCAAACAGACGTTTACGCGAGAAGGTTGCCGCAAttt > 1:507073/1‑68 (MQ=255) gCCCATACCAGCGAGGTATAACCAAACAGACGTTTACGCGAGAAGGTTGCCGCAATTTCGGAGAacaca < 1:1379343/69‑1 (MQ=255) gCCCATACCAGCGAGGTATAACCAAACAGACGTTTACGCGAGAAGGTTGCCGCAATTTCGGAGAacaca < 1:528774/69‑1 (MQ=255) gCCCATACCAGCGAGGTATAACCAAACAGACGTTTACGCGAGAAGGTTGCCGCAATTTCGGAGAacaca < 1:1678942/69‑1 (MQ=255) gCCCATACCAGCGAGGTATAACCAAACAGACGTTTACGCGAGAAGGTTGCCGCAATTTCGGAGAacaca < 1:2099453/69‑1 (MQ=255) gCCCATACCAGCGAGGTATAACCAAACAGACGTTTACGCGAGAAGGTTGCCGCAATTTCGGAGAacaca < 1:2828722/69‑1 (MQ=255) gCCCATACCAGCGAGGTATAACCAAACAGACGTTTACGCGAGAAGGTTGCCGCAATTTCGGAGAacaca < 1:1118028/69‑1 (MQ=255) ccAGCGAGGTATAACCAAACAGCCGTTTACGCGAGAAGGTTGCCGCAATTTCGGAGAACACACCGaa > 1:2205481/1‑67 (MQ=255) ccAGCGAGGTATAACCAAACAGACGTTTACGCGAGAAGGTTGCCGCAATTTCGGAGAACACACCGaa > 1:879773/1‑67 (MQ=255) ccAGCGAGGTATAACCAAACAGACGTTTACGCGAGAAGGTTGCCGCAATTTCGGAGAACACACCGaa > 1:1668469/1‑67 (MQ=255) ccAGCGAGGTATAACCAAACAGACGTTTACGCGAGAAGGTTGCCGCAATTTCGGAGAACACACCGaa > 1:2973340/1‑67 (MQ=255) ccAGCGAGGTATAACCAAACAGACGTTTACGCGAGAAGGTTGCCGCAATTTCGGAGAACACACCGaa > 1:1904256/1‑67 (MQ=255) cAGCGAGGTATAACCAAACAGACGTTTACGCGAGAAGGTTGCCGCAATTTCGGAGAACACACCGAAAAc > 1:2385297/1‑69 (MQ=255) cAGCGAGGTATAACCAAACAGACGTTTACGCGAGAAGGTTGCCGCAATTTCGGAGAACACACCGAAAAc > 1:3376075/1‑69 (MQ=255) cAGCGAGGTATAACCAAACAGACGTTTACGCGAGAAGGTTGCCGCAATTTCGGAGAACACACCGAAAAc > 1:2314370/1‑69 (MQ=255) cAGCGAGGTATAACCAAACAGACGTTTACGCGAGAAGGTTGCCGCAATTTCGGAGAACACACCGAAAAc > 1:2262286/1‑69 (MQ=255) cAGCGAGGTATAACCAAACAGACGTTTACGCGAGAAGGTTGCCGCAATTTCGGAGAACACACCGAAAAc > 1:1208032/1‑69 (MQ=255) cAGCGAGGTATAACCAAACAGACGTTTACGCGAGAAGGTTGCCGCAATTTCGGAGAACACACCGAAAAc > 1:1166289/1‑69 (MQ=255) gTATAACCAAACAGACGTTTACGCGAGAAGGTTGCCGCAATTTCGGAGAACACACCGAAAAcaggcagg < 1:559651/69‑1 (MQ=255) gTATAACCAAACAGACGTTTACGCGAGAAGGTTGCCGCAATTTCGGAGAACACACCGAAAAcaggcagg < 1:648857/69‑1 (MQ=255) gTATAACCAAACAGACGTTTACGCGAGAAGGTTGCCGCAATTTCGGAGAACACACCGAAAAcaggcagg < 1:1353640/69‑1 (MQ=255) taACCAAACAGACGTTTACGCGAGAAGGTTGCCGCAATTTCGGAGAACACACCGAAAAca < 1:1687562/60‑1 (MQ=255) taACCAAACAGACGTTTACGCGAGAAGGTTGCCGCAATTTCGGAGAACACACCGAAAAca < 1:2027416/60‑1 (MQ=255) taACCAAACAGACGTTTACGCGAGAAGGTTGCCGCAATTTCGGAGAACACACCGAAAAca < 1:2065618/60‑1 (MQ=255) taACCAAACAGACGTTTACGCGAGAAGGTTGCCGCAATTTCGGAGAACACACCGAAAAca < 1:1655088/60‑1 (MQ=255) taACCAAACAGACGTTTACGCGAGAAGGTTGCCGCAATTTCGGAGAACACACCGAAAAca < 1:3320857/60‑1 (MQ=255) taACCAAACAGACGTTTACGCGAGAAGGTTGCCGCAATTTCGGAGAACACACCGAAAAca < 1:1040519/60‑1 (MQ=255) taACCAAACAGACGTTTACGCGAGAAGGTTGCCGCAATTTCGGAGAACACACCGAAAAca < 1:916273/60‑1 (MQ=255) taACCAAACAGACGTTTACGCGAGAAGGTTGCCGCAATTTCGGAGAACACACCGAAAACAGGcaggatc > 1:727378/1‑69 (MQ=255) taACCAAACAGACGTTTACGCGAGAAGGTTGCCGCAATTTCGGAGAACACACCGAAAACAGGcaggatc > 1:3199077/1‑69 (MQ=255) taACCAAACAGACGTTTACGCGAGAAGGTTGCCGCAATTTCGGAGAACACACCGAAAACAGGcaggatc > 1:1118027/1‑69 (MQ=255) taACCAAACAGACGTTTACGCGAGAAGGTTGCCGCAATTTCGGAGAACACACCGAAAACAGGcaggatc > 1:2929877/1‑69 (MQ=255) taACCAAACAGACGTTTACGCGAGAAGGTTGCCGCAATTTCGGAGAACACACCGAAAACAGGcaggatc > 1:117007/1‑69 (MQ=255) taACCAAACAGACGTTTACGCGAGAAGGTTGCCGCAATTTCGGAGAACACACCGAAAACAGGcaggatc > 1:1303607/1‑69 (MQ=255) taACCAAACAGACGTTTACGCGAGAAGGTTGCCGCAATTTCGGAGAACACACCGAAAACAGGcaggatc > 1:1571211/1‑69 (MQ=255) taACCAAACAGACGTTTACGCGAGAAGGTTGCCGCAATTTCGGAGAACACACCGAAAACAGGcaggatc > 1:512504/1‑69 (MQ=255) taACCAAACAGACGTTTACGCGAGAAGGTTGCCGCAATTTCGGAGAACACACCGAAAACAGGcaggatc > 1:760789/1‑69 (MQ=255) taACCAAACAGACGTTTACGCGAGAAGGTTGCCGCAATTTCGGAGAACACACCGAAAACAGGcaggatc > 1:853535/1‑69 (MQ=255) taACCAAACAGACGTTTACGCGAGAAAGTTGCCGCAATTTCGGAGAACACACCGAATACAGGcagga > 1:2139375/1‑67 (MQ=255) taACCAAACAGACGTTTACGCGAGAAAGTTGCCGCAATTTCGGAGAACACACCGAATACAGGcagga > 1:1098369/1‑67 (MQ=255) taACCAAACAGACGTTTACGCGAGAAAGTTGCCGCAATTTCGGAGAACACACCGAATACAGGcagga > 1:3058169/1‑67 (MQ=255) taACCAAACAGACGTTTACGCGAGAAAGTTGCCGCAATTTCGGAGAACACACCGAATACAGGcagga > 1:687786/1‑67 (MQ=255) taACCAAACAGACGTTTACGCGAGAAAGTTGCCGCAATTTCGGAGAACACACCGAATACAGGcagga > 1:553276/1‑67 (MQ=255) taACCAAACAGACGTTTACGCGAGAAAGTTGCCGCAATTTCGGAGAACACACCGAATACAGGcagga > 1:2802471/1‑67 (MQ=255) taACCAAACAGACGTTTACGCGAGAAAGTTGCCGCAATTTCGGAGAACACACCGAATACAGGcagga > 1:154508/1‑67 (MQ=255) taACCAAACAGACGTTTACGCGAGAAAGTTGCCGCAATTTCGGAGAACACACCGAATACAGGcagga > 1:413822/1‑67 (MQ=255) taACCAAACAGACGTTTACGCGAGAAAGTTGCCGCAATTTCGGAGAACACACCGAATACAGGcagga > 1:477082/1‑67 (MQ=255) taACCAAACAGACGTTTACGCGAGAAAGTTGCCGCAATTTCGGAGAACACACCGAATACAGGcagga > 1:1329590/1‑67 (MQ=255) aaCCAAACAGACGTTTACGCGAGAAGGTTGCCGCAATTTCGGAGAACACACCGAAAAca < 1:2351565/59‑1 (MQ=255) aCCAAACAGACGTTTACGCGAGAAGGTTGCCGCAATTTCGGAGAACACACCGAAAACAGGcaggatcag > 1:3229196/1‑69 (MQ=255) aCCAAACAGACGTTTACGCGAGAAGGTTGCCGCAATTTCGGAGAACACACCGAAAACAGGcaggatcag > 1:996032/1‑69 (MQ=255) aCCAAACAGACGTTTACGCGAGAAGGTTGCCGCAATTTCGGAGAACACACCGAAAACAGGcaggatcag > 1:784576/1‑69 (MQ=255) aCCAAACAGACGTTTACGCGAGAAGGTTGCCGCAATTTCGGAGAACACACCGAAAACAGGcaggatcag > 1:1391014/1‑69 (MQ=255) aCCAAACAGACGTTTACGCGAGAAGGTTGCCGCAATTTCGGAGAACACACCGAAAACAGGcaggatcag > 1:1905962/1‑69 (MQ=255) cAGACGTTTACGCGAGAAAGTTGCCGCAATTTCGGAGAACACACCGAATAGAGGCAGGATCAAGATGTa < 1:1417637/69‑1 (MQ=255) cAGACGTTTACGCGAGAAAGTTGCCGCAATTTCGGAGAACACACCGAATACAGGCAGGATCAAGATGTa < 1:2985949/69‑1 (MQ=255) cAGACGTTTACGCGAGAAAGTTGCCGCAATTTCGGAGAACACACCGAATACAGGCAGGATCAAGATGTa < 1:1222153/69‑1 (MQ=255) cAGACGTTTACGCGAGAAAGTTGCCGCAATTTCGGAGAACACACCGAATACAGGCAGGATCAAGATGTa < 1:1248693/69‑1 (MQ=255) cAGACGTTTACGCGAGAAAGTTGCCGCAATTTCGGAGAACACACCGAATACAGGCAGGATCAAGATGTa < 1:1482094/69‑1 (MQ=255) cAGACGTTTACGCGAGAAAGTTGCCGCAATTTCGGAGAACACACCGAATACAGGCAGGATCAAGATGTa < 1:795328/69‑1 (MQ=255) cAGACGTTTACGCGAGAAAGTTGCCGCAATTTCGGAGAACACACCGAATACAGGCAGGATCAAGATGTa < 1:1488099/69‑1 (MQ=255) cAGACGTTTACGCGAGAAAGTTGCCGCAATTTCGGAGAACACACCGAATACAGGCAGGATCAAGATGTa < 1:1532364/69‑1 (MQ=255) cAGACGTTTACGCGAGAAAGTTGCCGCAATTTCGGAGAACACACCGAATACAGGCAGGATCAAGATGTa < 1:1727783/69‑1 (MQ=255) cAGACGTTTACGCGAGAAAGTTGCCGCAATTTCGGAGAACACACCGAATACAGGCAGGATCAAGATGTa < 1:1944079/69‑1 (MQ=255) cAGACGTTTACGCGAGAAAGTTGCCGCAATTTCGGAGAACACACCGAATACAGGCAGGATCAAGATGTa < 1:1947021/69‑1 (MQ=255) cAGACGTTTACGCGAGAAAGTTGCCGCAATTTCGGAGAACACACCGAATACAGGCAGGATCAAGATGTa < 1:2171688/69‑1 (MQ=255) cAGACGTTTACGCGAGAAAGTTGCCGCAATTTCGGAGAACACACCGAATACAGGCAGGATCAAGATGTa < 1:242473/69‑1 (MQ=255) cAGACGTTTACGCGAGAAAGTTGCCGCAATTTCGGAGAACACACCGAATACAGGCAGGATCAAGATGTa < 1:388163/69‑1 (MQ=255) cAGACGTTTACGCGAGAAAGTTGCCGCAATTTCGGAGAACACACCGAATACAGGCAGGATCAAGATGTa < 1:299987/69‑1 (MQ=255) cAGACGTTTACGCGAGAAAGTTGCCGCAATTTCGGAGAACACACCGAATACAGGCAGGATCAAGATGTa < 1:2915767/69‑1 (MQ=255) cAGACGTTTACGCGAGAAAGTTGCCGCAATTTCGGAGAACACACCGAATACAGGCAGGATCAAGATGTa < 1:329545/69‑1 (MQ=255) cAGACGTTTACGCGAGAAAGTTGCCGCAATTTCGGAGAACACACCGAATACAGGCAGGATCAAGATGTa < 1:3320478/69‑1 (MQ=255) cAGACGTTTACGCGAGAAAGTTGCCGCAATTTCGGAGAACACACCGAATACAGGCAGGATCAAGATGTa < 1:263909/69‑1 (MQ=255) cAGACGTTTACGCGAGAAAGTTGCCGCAATTTCGGAGAACACACCGAATACAGGCAGGATCAAGATGTa < 1:353481/69‑1 (MQ=255) cAGACGTTTACGCGAGAAAGTTGCCGCAATTTCGGAGAACACACCGAATACAGGCAGGATCAAGATGTa < 1:392209/69‑1 (MQ=255) cAGACGTTTACGCGAGAAAGTTGCCGCAATTTCGGAGAACACACCGAATACAGGCAGGATCAAGATGTa < 1:2586721/69‑1 (MQ=255) cAGACGTTTACGCGAGAAAGTTGCCGCAATTTCGGAGAACACACCGAATACAGGCAGGATCAAGATGTa < 1:45413/69‑1 (MQ=255) cAGACGTTTACGCGAGAAAGTTGCCGCAACTTCGGAGAACACACCGAATACAGGCAGGATCAAGATGTa < 1:1957854/69‑1 (MQ=255) aGACGTTTACGCGAGAAGGTTGCCGCAATTTCGGAGAACACACCGAAAAcaggcagg < 1:2222585/57‑1 (MQ=255) gTTTACGCGAGAAGGTTGCCGCAATTTCGGAGAACACACCGAAAACAGGCAGGATCAGGATGTAAACtt > 1:1856277/1‑69 (MQ=255) cgAGAAAGTTGCCGCAATTTCGGAGAACACACCGAATACAGGCAGGATCAAGATGTAAACTTCCGGGt < 1:244358/68‑1 (MQ=255) cgAGAAAGTTGCCGCAATTTCGGAGAACACACCGAATACAGGCAGGATCAAGATGTAAACTTCCGGGt < 1:3391965/68‑1 (MQ=255) cgAGAAAGTTGCCGCAATTTCGGAGAACACACCGAATACAGGCAGGATCAAGATGTAAACTTCCGGGt < 1:2795747/68‑1 (MQ=255) cgAGAAAGTTGCCGCAATTTCGGAGAACACACCGAATACAGGCAGGATCAAGATGTAAACTTCCGGGt < 1:541417/68‑1 (MQ=255) cgAGAAAGTTGCCGCAATTTCGGAGAACACACCGAATACAGGCAGGATCAAGATGTAAACTTCCGGGt < 1:457929/68‑1 (MQ=255) | GACAGCACGGTGATACAGACGGTTGCCCATACCAGCGAGGTATAACCAAACAGACGTTTACGCGAGAAGGTTGCCGCAATTTCGGAGAACACACCGAAAACAGGCAGGATCAGGATGTAAACTTCCGGGT > minE/278131‑278260 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |