| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 278,904 | C→T | 64.2% | L69L (TTG→TTA) | cyoB ← | cytochrome o ubiquinol oxidase subunit I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 278,904 | 0 | C | T | 64.2% | 41.3 / 39.1 | 67 | L69L (TTG→TTA) | cyoB | cytochrome o ubiquinol oxidase subunit I |
| Reads supporting (aligned to +/- strand): ref base C (10/14); new base T (28/15); total (38/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.65e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.44e-01 | |||||||||||
GAGCCTGCTGGCTACGCATCATAATGGCGTCAGCAAAACCACGCAGCAACATCACAATCGCCACGATGATATACATGATACCGAGGCGTTTATGGTCGACGG > minE/278858‑278959 | gagCCTGCTGGCTACGCATCATAATGGCGTCAGCAAAACCACGCAGCAACATCACAATCGCCACgatga < 1:3295949/69‑1 (MQ=255)gagCCTGCTGGCTACGCATCATAATGGCGTCAGCAAAACCACGCAGCAACATCACAATCGCCACgatga < 1:2075149/69‑1 (MQ=255)gagCCTGCTGGCTACGCATCATAATGGCGTCAGCAAAACCACGCAGCAACAACACAATCGCCACgatga < 1:922717/69‑1 (MQ=255) ccTGCTGGCTACGCATCATAATGGCGTCAGCAAAACCACGCAGCAACATCACAATCGCCACGATGata > 1:928628/1‑68 (MQ=255) ccTGCTGGCTACGCATCATAATGGCGTCAGCAAAACCACGCAGCAACATCACAATCGCCACGATGata > 1:1770453/1‑68 (MQ=255) ggCTACGCATCATAATGGCGTCAGCAAAACCACGCAGCAACATCACAATCGCCACGATGATATACAt < 1:2642316/67‑1 (MQ=255) ggCTACGCATCATAATGGCGTCAGCAAAACCACGCAGCAACATCACAATCGCCACGATGATATACAt < 1:999159/67‑1 (MQ=255) ggCTACGCATCATAATGGCGTCAGCAAAACCACGCAGCAACATCACAATCGCCACGATGATATACAt < 1:3115307/67‑1 (MQ=255) ggCTACGCATCATAATGGCGTCAGCAAAACCACGCAGCAACATCACAATCGCCACGATGATATACAt < 1:2968220/67‑1 (MQ=255) ggCTACGCATCATAATGGCGTCAGCAAAACCACGCAGCAACATCACAATCGCCACGATGATATACAt < 1:2928116/67‑1 (MQ=255) ggCTACGCATCATAATGGCGTCAGCAAAACCACGCAGCAACATCACAATCGCCACGATGATATACAt < 1:2583941/67‑1 (MQ=255) ggCTACGCATCATAATGGCGTCAGCAAAACCACGCAGCAACATCACAATCGCCACGATGATATACAt < 1:1114356/67‑1 (MQ=255) ggCTACGCATCATAATGGCGTCAGCAAAACCACGCAGCAACATCACAATCGCCACGATGATATACAt < 1:1200972/67‑1 (MQ=255) tACGCATCATAATGGCGTCAGCAAAACCACGCAGCAACATCACAATCGCCACGATGATATATATGATa > 1:2245009/1‑68 (MQ=255) tACGCATCATAATGGCGTCAGCAAAACCACGCAGCAACATCACAATCGCCACGATGATATACATGATa > 1:2704193/1‑68 (MQ=255) tACGCATCATAATGGCGTCAGCAAAACCACGCAGCAACATCACAATCGCCACGATGATATACATGATa > 1:2437405/1‑68 (MQ=255) tACGCATCATAATGGCGTCAGCAAAACCACGCAGCAACATCACAATCGCCACGATGATATACATGATa > 1:3153137/1‑68 (MQ=255) tACGCATCATAATGGCGTCAGCAAAACCACGCAGCAACATCACAATCGCCACGATGATATACATGATa > 1:2148368/1‑68 (MQ=255) tACGCATCATAATGGCGTCAGCAAAACCACGCAGCAACATCACAATCGCCACGATGATATACATGATa > 1:1783802/1‑68 (MQ=255) atAATGGCGTCAGCAAACCCACGCAGTAACATCACAATCGCCACGATGATATACATGATACCGAGGCg > 1:2624016/1‑68 (MQ=255) atAATGGCGTCAGCAAAACCACGCAGTAACATCACAATCGCCACgatgat > 1:2650699/1‑50 (MQ=255) atAATGGCGTCAGCAAAACCACGCAGTAACATCACAATCGCCACGATGATATACATGATACCGAgg > 1:3029980/1‑66 (MQ=255) atAATGGCGTCAGCAAAACCACGCAGTAACATCACAATCGCCACGATGATATACATGATACCGAGGCg > 1:897355/1‑68 (MQ=255) atAATGGCGTCAGCAAAACCACGCAGTAACATCACAATCGCCACGATGATATACATGATACCGAGGCg > 1:1740130/1‑68 (MQ=255) atAATGGCGTCAGCAAAACCACGCAGTAACATCACAATCGCCACGATGATATACATGATACCGAGGCg > 1:2766094/1‑68 (MQ=255) atAATGGCGTCAGCAAAACCACGCAGTAACATCACAATCGCCACGATGATATACATGATACCGAGGCg > 1:1406489/1‑68 (MQ=255) atAATGGCGTCAGCAAAACCACGCAGTAACATCACAATCGCCACGATGATATACATGATACCGAGGCg > 1:3030597/1‑68 (MQ=255) atAATGGCGTCAGCAAAACCACGCAGTAACATCACAATCGCCACGATGATATACATGATACCGAGGCg > 1:3108862/1‑68 (MQ=255) atAATGGCGTCAGCAAAACCACGCAGTAACATCACAATCGCCACGATGATATACATGATACCGAGGCg > 1:3147027/1‑68 (MQ=255) atAATGGCGTCAGCAAAACCACGCAGTAACATCACAATCGCCACGATGATATACATGATACCGAGGCg > 1:736943/1‑68 (MQ=255) atAATGGCGTCAGCAAAACCACGCAGTAACATCACAATCGCCACGATGATATACATGATACCGAGGCg > 1:796815/1‑68 (MQ=255) atAATGGCGTCAGCAAAACCACGCAGTAACATCACAATCGCCACGATGATATACATGATACCGAGGCg > 1:3285032/1‑68 (MQ=255) atAATGGCGTCAGCAAAACCACGCAGTAACATCACAATCGCCACGATGATATACATGATACCGAGGCg > 1:781851/1‑68 (MQ=255) atAATGGCGTCAGCAAAACCACGCAGTAACATCACAATCGCCACGATGATATACATGATACCGAGGCg > 1:1334822/1‑68 (MQ=255) atAATGGCGTCAGCAAAACCACGCAGTAACATCACAATCGCCACGATGATATACATGATACCGAGGCg > 1:451692/1‑68 (MQ=255) atAATGGCGTCAGCAAAACCACGCAGTAACATCACAATCGCCACGATGATATACATGATACCGAGGCg > 1:561616/1‑68 (MQ=255) atAATGGCGTCAGCAAAACCACGCAGTAACATCACAATCGCCACGATGATATACATGATACCGAGGCg > 1:606915/1‑68 (MQ=255) atAATGGCGTCAGCAAAACCACGCAGTAACATCACAATCGCCACGATGATATACATGATACCGAGGCg > 1:1546592/1‑68 (MQ=255) atAATGGCGTCAGCAAAACCACGCAGTAACATCACAATCGCCACGATGATATACATGATACCGAGGCg > 1:1663470/1‑68 (MQ=255) atAATGGCGTCAGCAAAACCACGCAGTAACATCACAATCGCCACGATGATATACATGATACCGAGGCg > 1:1747734/1‑68 (MQ=255) atAATGGCGTCAGCAAAACCACGCAGTAACATCACAATCGCCACGATGATATACATGATACCGAGGCg > 1:1632872/1‑68 (MQ=255) atAATGGCGTCAGCAAAACCACGCAGTAACATCACAATCGCCACGATGATATACATGATACCGAGGCg > 1:1892522/1‑68 (MQ=255) atAATGGCGTCAGCAAAACCACGCAGTAACATCACAATCGCCACGATGATATACATGATACCGAGGCg > 1:1975872/1‑68 (MQ=255) atAATGGCGTCAGCAAAACCACGCAGTAACATCACAATCGCCACGATGATATACATGATACCGAGGCg > 1:246991/1‑68 (MQ=255) atAATGGCGTCAGCAAAACCACGCAGTAACATCACAATCGCCACGATGATATACATGATACCGAGGCg > 1:2626674/1‑68 (MQ=255) atAATGGCGTCAGCAAAACCACGCAGTAACATCACAATCGCCACGATGATATACATGATACCGAGGCg > 1:1030168/1‑68 (MQ=255) atAATGCCGTCAGCAAAACCACGCAGTAACATCACAATCGCCACGATGATATACATGATACCGAGGCg > 1:5731/1‑68 (MQ=255) aTGGCGTCAGCAAAACCACGCAGCAACATCACAATCGCCACGATGATATACAt < 1:778482/53‑1 (MQ=255) aTGGCGTCAGCAAAACCACGCAGCAACATCACAATCGCCACGATGATATACAt < 1:64424/53‑1 (MQ=255) aTGGCGTCAGCAAAACCACGCAGCAACATCACAATCGCCACGATGATATACATGATACCGAGGCGTTTa > 1:1258025/1‑69 (MQ=255) aTGGCGTCAGCAAAACCACGCAGCAACATCACAATCGCCACGATGATATACATGATACCGAGGCGTTTa > 1:2652101/1‑69 (MQ=255) gTCAGCAAAACCACGCAGCAACATCACAATCGCCACGATGATATACATGATACCGAGGCGTTTATGGTc < 1:2211906/69‑1 (MQ=255) cAAAACCACGCAGTAACATCACAATCGCCACGATGATATACATGATACCGAGGCGTTTATGGTCGACgg < 1:2789557/69‑1 (MQ=255) cAAAACCACGCAGTAACATCACAATCGCCACGATGATATACATGATACCGAGGCGTTTATGGTCGACgg < 1:1460579/69‑1 (MQ=255) cAAAACCACGCAGTAACATCACAATCGCCACGATGATATACATGATACCGAGGCGTTTATGGTCGACgg < 1:2922444/69‑1 (MQ=255) cAAAACCACGCAGTAACATCACAATCGCCACGATGATATACATGATACCGAGGCGTTTATGGTCGACgg < 1:1419033/69‑1 (MQ=255) cAAAACCACGCAGTAACATCACAATCGCCACGATGATATACATGATACCGAGGCGTTTATGGTCGACgg < 1:1972855/69‑1 (MQ=255) cAAAACCACGCAGTAACATCACAATCGCCACGATGATATACATGATACCGAGGCGTTTATGGTCGACgg < 1:750535/69‑1 (MQ=255) cAAAACCACGCAGTAACATCACAATCGCCACGATGATATACATGATACCGAGGCGTTTATGGTCGACgg < 1:3020340/69‑1 (MQ=255) cAAAACCACGCAGTAACATCACAATCGCCACGATGATATACATGATACCGAGGCGTTTATGGTCGACgg < 1:200126/69‑1 (MQ=255) cAAAACCACGCAGTAACATCACAATCGCCACGATGATATACATGATACCGAGGCGTTTATGGTCGACgg < 1:2036302/69‑1 (MQ=255) cAAAACCACGCAGTAACATCACAATCGCCACGATGATATACATGATACCGAGGCGTTTATGGTCGACgg < 1:542311/69‑1 (MQ=255) cAAAACCACGCAGTAACATCACAATCGCCACGATGATATACATGATACCGAGGCGTTTATGGTCGACgg < 1:2120399/69‑1 (MQ=255) cAAAACCACGCAGTAACATCACAATCGCCACGATGATATACATGATACCGAGGCGTTTATGGTCGACgg < 1:2371379/69‑1 (MQ=255) cAAAACCACGCAGTAACATCACAATCGCCACGATGATATACATGATACCGAGGCGTTTATGGTCGACgg < 1:3141656/69‑1 (MQ=255) cAAAACCACGCAGTAACATCACAATCGCCACGATGATATACATGATACCGAGGCGTTTATGGTCGACgg < 1:1374916/69‑1 (MQ=255) cAAAACCACGCAGTAACATCACAATCGCCACGATGATATACATGATACCGAGGCGTTTATGGTCGACgg < 1:2547975/69‑1 (MQ=255) | GAGCCTGCTGGCTACGCATCATAATGGCGTCAGCAAAACCACGCAGCAACATCACAATCGCCACGATGATATACATGATACCGAGGCGTTTATGGTCGACGG > minE/278858‑278959 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |