| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 285,523 | T→C | 56.2% | F126F (TTT→TTC) | clpP → | proteolytic subunit of ClpA‑ClpP and ClpX‑ClpP ATP‑dependent serine proteases |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 285,523 | 0 | T | C | 56.2% | 19.1 / 54.4 | 73 | F126F (TTT→TTC) | clpP | proteolytic subunit of ClpA‑ClpP and ClpX‑ClpP ATP‑dependent serine proteases |
| Reads supporting (aligned to +/- strand): ref base T (11/21); new base C (28/13); total (39/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.97e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGGCCTCGATGGGCGCTTTCTTGCTGACCGCAGGGGCAAAAGGTAAACGTTTTTGCCTGCCGAATTCGCGCGTGATGATTCACCAACCGTTGGGCGGCTACCAGGGCCAGGCGACCGAT > minE/285471‑285589 | cggcCTCGATGGGCGCTTTCTTGCTGACCGCAGGGGCAAAAGGTAAACGTTTTTGCCTGCCGAATTcgc > 1:2564828/1‑69 (MQ=255)cggcCTCGATGGGCGCTTTCTTGCTGACCGCAGGGGCAAAAGGTAAACGTTTTTGCCTGCCGAATTcgc > 1:2063067/1‑69 (MQ=255) cGATGGGTGCTTTCTTGCTGACTGCAGGGGCAAAAGGTAAACGTTTCtgcc > 1:794538/1‑51 (MQ=255) cGATGGGTGCTTTCTTGCTGACTGCAGGGGCAAAAGGTAAACGTTTCtgcc > 1:1919444/1‑51 (MQ=255) cGATGGGTGCTTTCTTGCTGACTGCAGGGGCAAAAGGTAAACGTTTCtgcc > 1:1334192/1‑51 (MQ=255) cGATGGGTGCTTTCTTGCTGACTGCAGGGGCAAAAGGTAAACGTTTCtgcc > 1:1787675/1‑51 (MQ=255) cGATGGGTGCTTTCTTGCTGACTGCAGGGGCAAAAGGTAAACGTTTCtgcc > 1:1460889/1‑51 (MQ=255) cGATGGGTGCTTTCTTGCTGACTGCAGGGGCAAAAGGTAAACGTTTCtgcc > 1:3303365/1‑51 (MQ=255) ggCGCTTTCTTGCTGACCGCAGGGGCAAAAGGTAAACGTTTTTGCCTGCCGAATTCGCGCGTGATGAtt < 1:2734328/69‑1 (MQ=255) ggCGCTTTCTTGCTGACCGCAGGGGCAAAAGGTAAACGTTTTTGCCTGCCGAATTCGCGCGTGATGAtt < 1:2646528/69‑1 (MQ=255) ggCGCTTTCTTGCTGACCGCAGGGGCAAAAGGTAAACGTTTTTGCCTGCCGAATTCGCGCGTGATGAtt < 1:1839263/69‑1 (MQ=255) ggCGCTTTCTTGCTGACCGCAGGGGCAAAAGGTAAACGTTTTTGCCTGCCGAATTCGCGCGTGATGAtt < 1:1685265/69‑1 (MQ=255) ggCGCTTTCTTGCTGACCGCAGGGGCAAAAGGTAAACGTTTTTGCCTGCCGAATTCGCGCGTGATGAtt > 1:923736/1‑69 (MQ=255) ggCGCTTTCTTGCTGACCGCAGGGGCAAAAGGTAAACGTTTTTGCCTGCCGAATTCGCGCGTGATGAtt < 1:1106756/69‑1 (MQ=255) ggCGCTTTCTTGCTGACCGCAGGGGCAAAAGGTAAACGTTTTTGCCTGCCGAATTCGCGCGTGATGAtt < 1:1282487/69‑1 (MQ=255) cGCAGGGGCAAAAGGTAAACGTTTTTGCCTGCCGAATTCGCGCGTGATGATTCACCAACCGTTGggcg > 1:2149242/1‑68 (MQ=255) cGCAGGGGCAAAAGGTAAACGTTTTTGCCTGCCGAATTCGCGCGTGATGATTCACCAACCGTTGggcg > 1:120605/1‑68 (MQ=255) cGCAGGGGCAAAAGGTAAACGTTTTTGCCTGACGAATTCGCGGGTGATGATTCACCAACCGTTGggcg > 1:1861600/1‑68 (MQ=255) gCAGGGGCAAAAGGTAAACGTTTTTGCCTGCCGAATTCGCGCGTGATGATTCACCAACCGTTGggcggc < 1:228052/69‑1 (MQ=255) cAGGGTCAAAAGGTAAACGTTTCTGCCTGCCGAAtt > 1:236979/1‑36 (MQ=37) cAGGGGCAAAAGGTAAACGTTTCTGCCTGCCGAATTCGCGCGt > 1:2933870/1‑43 (MQ=255) cAGGGGCAAAAGGTAAACGTTTCTGCCTGCCGAATTCGCGCGt > 1:1676276/1‑43 (MQ=255) cAGGGGCAAAAGGTAAACGTTTCTGCCTGCCGAATTCGCGCGt > 1:842085/1‑43 (MQ=255) cAGGGGCAAAAGGTAAACGTTTCTGCCTGCCGAATTCGCGCGt > 1:2410691/1‑43 (MQ=255) cAGGGGCAAAAGGTAAACGTTTCTGCCTGCCGAATTCGCGCGt > 1:2825842/1‑43 (MQ=255) cAGGGGCAAAAGGTAAACGTTTCTGCCTGCCGAATTCGCGCGt > 1:2824300/1‑43 (MQ=255) cAGGGGCAAAAGGTAAACGTTTCTGCCTGCCGAATTCGCGCGt > 1:2814415/1‑43 (MQ=255) cAGGGGCAAAAGGTAAACGTTTCTGCCTGCCGAATTCGCGCGt > 1:2666778/1‑43 (MQ=255) cAGGGGCAAAAGGTAAACGTTTCTGCCTGCCGAATTCGCGCGt > 1:2679605/1‑43 (MQ=255) cAGGGGCAAAAGGTAAACGTTTCTGCCTGCCGAATTCGCGCGt > 1:2742283/1‑43 (MQ=255) cAGGGGCAAAAGGTAAACGTTTCTGCCTGCCGAATTCGCGCGt > 1:3435069/1‑43 (MQ=255) cAGGGGCAAAAGGTAAACGTTTCTGCCTGCCGAATTCGCGCGt > 1:3373894/1‑43 (MQ=255) cAGGGGCAAAAGGTAAACGTTTCTGCCTGCCGAATTCGCGCGt > 1:1737025/1‑43 (MQ=255) cAGGGGCAAAAGGTAAACGTTTCTGCCTGCCGAATTCGCGCGt > 1:3122085/1‑43 (MQ=255) cAGGGGCAAAAGGTAAACGTTTCTGCCTGCCGAATTCGCGCGt > 1:3063064/1‑43 (MQ=255) cAGGGGCAAAAGGTAAACGTTTCTGCCTGCCGAATTCGCGCGt > 1:1388422/1‑43 (MQ=255) cAGGGGCAAAAGGTAAACGTTTCTGCCTGCCGAATTCGCGCGt > 1:1808890/1‑43 (MQ=255) cAGGGGCAAAAGGTAAACGTTTCTGCCTGCCGAATTCGCGCGt > 1:2001051/1‑43 (MQ=255) cAGGGGCAAAAGGTAAACGTTTCTGCCTGCCGAATTCGCGCGt > 1:2051032/1‑43 (MQ=255) cAGGGGCAAAAGGTAAACGTTTCTGCCTGCCGAATTCGCGCGt > 1:2055122/1‑43 (MQ=255) cAGGGGCAAAAGGTAAACGTTTCTGCCTGACGAAATc > 1:488502/1‑37 (MQ=25) ggggCAAAAGGTAAACGTTTCTGCCTGGCGAATTCGCGCGTGATGATTCACCAGCCGTTGGGCGGCt < 1:2321910/67‑1 (MQ=255) ggggCAAAAGGTAAACGTTTCTGCCTGCCGAATTCGCGCGTTATGATTCACCAGCCGTTGGGCGGCt < 1:3018764/67‑1 (MQ=255) ggggCAAAAGGTAAACGTTTCTGCCTGCCGAATTCGCGCGTGATGATTCACCAGCCGTTGGGCGGCt < 1:2866279/67‑1 (MQ=255) ggggCAAAAGGTAAACGTTTCTGCCTGCCGAATTCGCGCGTGATGATTCACCAGCCGTTGGGCGGCt < 1:2888374/67‑1 (MQ=255) ggggCAAAAGGTAAACGTTTCTGCCTGCCGAATTCGCGCGTGATGATTCACCAGCCGTTGGGCGGCt < 1:3094659/67‑1 (MQ=255) ggggCAAAAGGTAAACGTTTCTGCCTGCCGAATTCGCGCGTGATGATTCACCAGCCGTTGGGCGGCt < 1:479302/67‑1 (MQ=255) ggggCAAAAGGTAAACGTTTCTGCCTGCCGAATTCGCGCGTGATGATTCACCAGCCGTTGGGCGGCt < 1:1039705/67‑1 (MQ=255) ggggCAAAAGGTAAACGTTTCTGCCTGCCGAATTCGCGCGTGATGATTCACCAGCCGTTGGGCGGCt < 1:2575272/67‑1 (MQ=255) ggggCAAAAGGTAAACGTTTCTGCCTGCCGAATTCGCGCGTGATGATTCACCAGCCGTTGGGCGGCt < 1:1113582/67‑1 (MQ=255) ggggCAAAAGGTAAACGTTTCTGCCTGCCGAATTCGCGCGTGATGATTCACCAGCCGTTGGGCGGCt < 1:2075028/67‑1 (MQ=255) ggggCAAAAGGTAAACGTTTCTGCCTGCCGAATTCGCGCGTGATGATTCACCAGCCGTTGGGCGGCt < 1:1782464/67‑1 (MQ=255) gggCAAAAGGTAAACGTTTCTGCCTGCCGAATTCGCGCGTGATGATTCACCAGCCGTTGGGCGGCt < 1:1767711/66‑1 (MQ=255) gCAAAAGGTAAACGTTTTTGCCTGCCGAATTCGCGCGTGATGATTCACCAACCGTTGGGCGGCTAc < 1:2523617/66‑1 (MQ=255) gCAAAAGGTAAACGTTTTTGCCTGCCGAATTCGCGCGTGATGATTCACCAACCGTTGGGCGGCTAc < 1:2399699/66‑1 (MQ=255) gCAAAAGGTAAACGTTTTTGCCTGCCGAATTCGCGCGTGATGATTCACCAACCGTTGGGCGGCTAc < 1:2063521/66‑1 (MQ=255) gCAAAAGGTAAACGTTTTTGCCTGCCGAATTCGCGCGTGATGATTCACCAACCGTTGGGCGGCTAc < 1:1825511/66‑1 (MQ=255) gCAAAAGGTAAACGTTTTTGCCTGCCGAATTCGCGCGTGATGATTCACCAACCGTTGGGCGGCTAc < 1:1757764/66‑1 (MQ=255) gCAAAAGGTAAACGTTTTTGCCTGCCGAATTCGCGCGTGATGATTCACCAACCGTTGGGCGGCTAc < 1:1594534/66‑1 (MQ=255) cAAAAGGTAAACGTTTTTGCCTGCCGAATTCGCGCGTGATGATTCACCAACCGTTGGGCGGCTAc < 1:2688866/65‑1 (MQ=255) ggTAAACGTTTTTGCCTGCCGAATTCGCGCGTGATGATTCACCAACCGTTGGGCGGCTACCAGGGCCAg < 1:711613/69‑1 (MQ=255) ggTAAACGTTTTTGCCTGCCGAATTCGCGCGTGATGATTCACCAACCGTTGGGCGGCTACCAGGGCCAg < 1:1175955/69‑1 (MQ=255) ggTAAACGTTTTTGCCTGCCGAATTCGCGCGTGATGATTCACCAACCGTTGGGCGGCTACCAGGGCCAg < 1:1532528/69‑1 (MQ=255) ggTAAACGTTTTTGCCTGCCGAATTCGCGCGTGATGATTCACCAACCGTTGGGCGGCTACCAGGGCCAg < 1:714777/69‑1 (MQ=255) ggTAAACGTTTTTGCCTGCCGAATTCGCGCGTGATGATTCACCAACCGTTGGGCGGCTACCAGGGCCAg < 1:836937/69‑1 (MQ=255) tAAACGTTTTTGCCTGCCGAATTCGCGCGTGATGATTCACCAACCGTTGGGCGGCTACCAGGGCCAgg > 1:2596654/1‑68 (MQ=255) tAAACGTTTTTGCCTGCCGAATTCGCGCGTGATGATTCACCAACCGTTGGGCGGCTACCAGGGCCAGGc > 1:1097105/1‑69 (MQ=255) tAAACGTTTTTGCCTGCCGAATTCGCGCGTGATGATTCACCAACCGTTGGGCGGCTACCAGGGCCAGGc > 1:528034/1‑69 (MQ=255) tAAACGTTTTTGCCTGCCGAATTCGCGCGTGATGATTCACCAACCGTTGGGCGGCTACCAGGGCCAGGc > 1:3373435/1‑69 (MQ=255) tAAACGTTTTTGCCTGCCGAATTCGCGCGTGATGATTCACCAACCGTTGGGCGGCTACCAGGGCCAGGc > 1:1561348/1‑69 (MQ=255) tAAACGTTTCTGCCTGCCGAAATCGCGCGTGATGATTCACCAGCCGTTGGGCGGCt < 1:950006/56‑1 (MQ=255) aaaCGTTTTTGCCTGCCGAATTCGCGCGTGATGATTCACCAACCGTTGGGCGGCTACCAGGGCCAg < 1:2951801/66‑1 (MQ=255) cGTTTTTGCCTGCCGATTTCGCGCGTGATGATTCACCAACCGTTGGGCGGCTACCAGGGCCAg < 1:2319577/63‑1 (MQ=255) ttttGCCTGCCGAATTCGCGCGTGATGATTCACCAACCGTTGGGCGGCTACCAGGGCCAGGCGACCGat < 1:2232957/69‑1 (MQ=255) | CGGCCTCGATGGGCGCTTTCTTGCTGACCGCAGGGGCAAAAGGTAAACGTTTTTGCCTGCCGAATTCGCGCGTGATGATTCACCAACCGTTGGGCGGCTACCAGGGCCAGGCGACCGAT > minE/285471‑285589 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |