| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 287,902 | T→C | 18.9% | S182S (TCT→TCC) | lon → | DNA‑binding ATP‑dependent protease La |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 287,902 | 0 | T | C | 18.9% | 126.0 / 19.2 | 84 | S182S (TCT→TCC) | lon | DNA‑binding ATP‑dependent protease La |
| Reads supporting (aligned to +/- strand): ref base T (30/38); new base C (2/14); total (32/52) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.25e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GACGATCCGGCGCGTCTGGCGGATACCATTGCTGCACATATGCCGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAATATCTGATGGCAATGATGGAATCGGAAATCGA > minE/287834‑287970 | gacgaTCCGGCGCGTCTGGCGGATACCATTGCTGCACATATGCCGCTGAAACTGGCTGACAAACAGTCt > 1:2019158/1‑69 (MQ=255)gacgaTCCGGCGCGTCTGGCGGATACCATTGCTGCACATATGCCGCTGAAACTGGCTGACAAACAGTCt > 1:1356809/1‑69 (MQ=255)gacgaTCCGGCGCGTCTGGCGGATACCATTGCTGCACATATGCCGCTGAAACTGGCTGACAAACAGTCt > 1:1428721/1‑69 (MQ=255)gacgaTCCGGCGCGTCTGGCGGATACCATTGCTGCACATATGCCGCTGAAACTGGCTGACAAACAGTCt > 1:1531605/1‑69 (MQ=255) cgaTCCGGCGCGTCTGGCGGATACCATTGCTGCACATATGCCGCTGAAACTGGCTGACAAACAGTCTg < 1:924234/68‑1 (MQ=255) gcgcGTCTGGCGGATACCATTGCTGCACATATGCCGCTGAAACTGGCTGACAAACAGTCTGTTCTGga < 1:1957119/68‑1 (MQ=255) gcgcGTCTGGCGGATACCATTGCTGCACATATGCCGCTGAAACTGGCTGACAAACAGTCTGTTCTGga < 1:902900/68‑1 (MQ=255) gcgcGTCTGGCGGATACCATTGCTGCACATATGCCGCTGAAACTGGCTGACAAACAGTCTGTTCTGga < 1:1685573/68‑1 (MQ=255) gcgcGTCTGGCGGATACCATTGCTGCACATATGCCGCTGAAACTGGCTGACAAACAGTCTGTTCTGga < 1:2141674/68‑1 (MQ=255) cTGGCGGATACCATTGCTGCACATATGCCGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTcc < 1:2492669/69‑1 (MQ=255) ccATTGCTGCACATATGCCGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTaacg > 1:245703/1‑69 (MQ=255) ccATTGCTGCACATATGCCGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTaacg > 1:954617/1‑69 (MQ=255) ccATTGCTGCACATATGCCGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTaacg > 1:3336197/1‑69 (MQ=255) ccATTGCTGCACATATGCCGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTaacg > 1:2453272/1‑69 (MQ=255) cATTGCTGCACATATGCCGCTGAAACTGGCTGACAAACAGTCCGTTCTGGAGATGt > 1:698230/1‑56 (MQ=255) cATTGCTGCACATATGCCGCTGAAACTGGCTGACAAACAGTCCGTTCTGGAGATGTCCGACGttcgt > 1:854781/1‑64 (MQ=255) gctgcACATATGCCGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAAGGAACGt > 1:2244096/1‑69 (MQ=255) gctgcACATATGCCGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGt > 1:2304169/1‑69 (MQ=255) gctgcACATATGCCGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGt > 1:2692656/1‑69 (MQ=255) gctgcACATATGCCGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGt > 1:766180/1‑69 (MQ=255) gctgcACATATGCCGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGt > 1:512170/1‑69 (MQ=255) gctgcACATATGCCGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGt > 1:192598/1‑69 (MQ=255) gctgcACATATGCCGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGt > 1:1002531/1‑69 (MQ=255) gctgcACATATGCCGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGt > 1:1894703/1‑69 (MQ=255) gctgcACATATGCCGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGt > 1:1704537/1‑69 (MQ=255) gctgcACATATGCCGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGt > 1:1386881/1‑69 (MQ=255) gctgcACATATGCCGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGt > 1:3370343/1‑69 (MQ=255) gctgcACATATGCCGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGt > 1:1253783/1‑69 (MQ=255) gctgcACATATGCCGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGt > 1:402377/1‑69 (MQ=255) gctgcACATATGCCGCTGAAACTGGCTGACAAACAGTCTGATCTGGAGATGTCCGACGTTAACGAACGt > 1:3139136/1‑69 (MQ=255) tatGCCGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAata < 1:3224732/69‑1 (MQ=255) tatGCCGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAata < 1:1080755/69‑1 (MQ=255) tatGCCGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAata < 1:3247558/69‑1 (MQ=255) tatGCCGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAata > 1:488023/1‑69 (MQ=255) tatGCCGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAata > 1:3309122/1‑69 (MQ=255) tatGCCGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAata < 1:711586/69‑1 (MQ=255) tatGCCGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAata > 1:3394466/1‑69 (MQ=255) tatGCCGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAata < 1:2434497/69‑1 (MQ=255) tatGCCGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAata > 1:116176/1‑69 (MQ=255) tatGCCGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAata < 1:970271/69‑1 (MQ=255) tatGCCGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAata > 1:1290222/1‑69 (MQ=255) tatGCCGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAata < 1:1809356/69‑1 (MQ=255) tatGCCGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAata < 1:1924479/69‑1 (MQ=255) tatGCCGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAata > 1:1833543/1‑69 (MQ=255) tatGCCGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAata < 1:823834/69‑1 (MQ=255) tatGCCGCTGAAACTGGATGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAata > 1:323157/1‑69 (MQ=255) tatGCAGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTaacgaac > 1:255515/1‑59 (MQ=255) tGCCGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGTc < 1:2537668/60‑1 (MQ=255) tGCCGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGTCt < 1:635738/61‑1 (MQ=255) tGCCGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGTCt < 1:696867/61‑1 (MQ=255) tGCCGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGTCt < 1:923005/61‑1 (MQ=255) tGCCGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGTCt < 1:808630/61‑1 (MQ=255) tGCCGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGTCt < 1:807906/61‑1 (MQ=255) tGCCGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGTCt < 1:1284243/61‑1 (MQ=255) tGCCGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGTCt < 1:474732/61‑1 (MQ=255) tGCCGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGTCt < 1:1263482/61‑1 (MQ=255) tGCCGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGTCt < 1:3389199/61‑1 (MQ=255) tGCCGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGTCt < 1:786253/61‑1 (MQ=255) tGCCGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGTCt < 1:425127/61‑1 (MQ=255) tGCCGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGTCt < 1:426827/61‑1 (MQ=255) ccGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAATATCt < 1:541261/68‑1 (MQ=255) ccGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAATATCt < 1:2906980/68‑1 (MQ=255) ccGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAATATCt < 1:2939634/68‑1 (MQ=255) ccGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAATATCt < 1:2740479/68‑1 (MQ=255) ccGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAATATCt < 1:2695313/68‑1 (MQ=255) ccGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAATATCt < 1:2363813/68‑1 (MQ=255) gAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAATATCTGATGGc < 1:917391/69‑1 (MQ=255) gAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAATATCTGATGGc < 1:3227748/69‑1 (MQ=255) aCTGGCTGACAAACAGTCCGTTCTGGAGATGTCCGACGTTAACTAACGTCTGGAATATCTGATGGCaa < 1:986803/68‑1 (MQ=255) aCTGGCTGACAAACAGTCCGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAATATCTGATGTCaa < 1:1863134/68‑1 (MQ=255) aCTGGCTGACAAACAGTCCGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAATATCTGATGGCaa < 1:336526/68‑1 (MQ=255) aCTGGCTGACAAACAGTCCGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAATATCTGATGGCaa < 1:1664194/68‑1 (MQ=255) aCTGGCTGACAAACAGTCCGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAATATCTGATGGCaa < 1:1782652/68‑1 (MQ=255) aCTGGCTGACAAACAGTCCGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAATATCTGATGGCaa < 1:83783/68‑1 (MQ=255) aCTGGCTGACAAACAGTCCGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAATATCTGATGGCaa < 1:1987061/68‑1 (MQ=255) aCTGGCTGACAAACAGTCCGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAATATCTGATGGCaa < 1:808374/68‑1 (MQ=255) aCTGGCTGACAAACAGTCCGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAATATCTGATGGCaa < 1:2409528/68‑1 (MQ=255) aCTGGCTGACAAACAGTCCGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAATATCTGATGGCaa < 1:752490/68‑1 (MQ=255) aCTGGCTGACAAACAGTCCGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAATATCTGATGGCaa < 1:2554330/68‑1 (MQ=255) aCTGGCTGACAAACAGTCCGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAATATCTGATGGCaa < 1:994930/68‑1 (MQ=255) aCTGGCTGACAAACAGTCCGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAATATCTGATGGCaa < 1:523880/68‑1 (MQ=255) aCTGGCTGACAAACAGTCCGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAATATCTGATGGCaa < 1:50056/68‑1 (MQ=255) cTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGTCt < 1:1881023/46‑1 (MQ=255) cAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGTCt < 1:1290135/42‑1 (MQ=255) tCTGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAATATCTGATGGCAATGATGGAATCGGAAATc > 1:994970/1‑69 (MQ=255) tCTGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAATATCTGATGGCAATGATGGAATCGGAAATc > 1:3264669/1‑69 (MQ=255) cTGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAATATCTGATGGCAATGATGGAATCGGAAATc > 1:2046927/1‑68 (MQ=255) cTGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAATATCTGATGGCAATGATGGAATCGGAAATCg > 1:1537883/1‑69 (MQ=255) tGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAATATCTGATGGCAATGATGGAATCGGAAATCGa < 1:1552037/69‑1 (MQ=255) tGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAATATCTGATGGCAATGATGGAATCGGAAATCGa < 1:2767624/69‑1 (MQ=255) | GACGATCCGGCGCGTCTGGCGGATACCATTGCTGCACATATGCCGCTGAAACTGGCTGACAAACAGTCTGTTCTGGAGATGTCCGACGTTAACGAACGTCTGGAATATCTGATGGCAATGATGGAATCGGAAATCGA > minE/287834‑287970 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |