| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 288,179 | T→C | 49.1% | L275L (TTG→CTG) | lon → | DNA‑binding ATP‑dependent protease La |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 288,179 | 0 | T | C | 49.1% | ‑5.4 / 51.0 | 58 | L275L (TTG→CTG) | lon | DNA‑binding ATP‑dependent protease La |
| Reads supporting (aligned to +/- strand): ref base T (15/14); new base C (19/9); total (34/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.83e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.86e-01 | |||||||||||
GCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAATGATGTCTCCGATGTCGGCAGAAGCGACCGTAGTGCGTGGTTATATCGACTGG > minE/288113‑288247 | gCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGt > 1:1920474/1‑67 (MQ=255)gCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGt > 1:1822801/1‑67 (MQ=255)gCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGt > 1:2668172/1‑67 (MQ=255) cccTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGt > 1:2846485/1‑66 (MQ=255) cccTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGt > 1:939897/1‑66 (MQ=255) cccTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGt > 1:88291/1‑66 (MQ=255) cccTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGCTGc < 1:3120060/69‑1 (MQ=255) cccTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGCTGc < 1:3273467/69‑1 (MQ=255) cccTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGCTGc < 1:3100277/69‑1 (MQ=255) cccTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGCTGc < 1:80277/69‑1 (MQ=255) cccTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGCTGc < 1:1421732/69‑1 (MQ=255) cccTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGCTGc < 1:3088472/69‑1 (MQ=255) cccTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGCTGc < 1:1165099/69‑1 (MQ=255) cccTGAAGCGCAAAATCGACGCGGCGAAGATGCAGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGt > 1:3120165/1‑66 (MQ=255) cccTGAAGCGCAAAATCGACGCGGCGAAGATGCAGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGt > 1:3123873/1‑66 (MQ=255) cacTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGt > 1:144695/3‑66 (MQ=255) ccTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGCTGc < 1:1833257/68‑1 (MQ=255) aTCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGCTGc < 1:1768383/55‑1 (MQ=255) tCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAatga > 1:2819035/1‑69 (MQ=255) tCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAatga > 1:2746955/1‑69 (MQ=255) tCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAatga > 1:1724562/1‑69 (MQ=255) tCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAatga > 1:2544084/1‑69 (MQ=255) tCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAatga > 1:3405265/1‑69 (MQ=255) cGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAatgat < 1:939216/69‑1 (MQ=255) cGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAatgat < 1:1140653/69‑1 (MQ=255) cGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAatgat < 1:3377502/69‑1 (MQ=255) cGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAatgat < 1:457602/69‑1 (MQ=255) cGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAatgat < 1:2622142/69‑1 (MQ=255) cGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAatgat < 1:2711336/69‑1 (MQ=255) aCGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAatgat < 1:426478/67‑1 (MQ=255) gcggcgAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAATGATGtct < 1:967045/69‑1 (MQ=255) cGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGCTGCAGAAGCTGAAAATGATGTCTCCg > 1:3241164/1‑59 (MQ=255) cGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGCTGCAGAAGCTGAAAATGATGTCTCCg > 1:3104029/1‑59 (MQ=255) cGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGCTGCAGAAGCTGAAAATGATGTCTCCg > 1:3429103/1‑59 (MQ=255) cGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGCTGCAGAAGCTGAAAATGATGTCTCCg > 1:50052/1‑59 (MQ=255) cGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGCTGCAGAAGCTGAAAATGATGTCTCCg > 1:51355/1‑59 (MQ=255) cGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGCTGCAGAAGCTGAAAATGATGTCTCCg > 1:571457/1‑59 (MQ=255) cGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGCTGCAGAAGCTGAAAATGATGTCTCCg > 1:601680/1‑59 (MQ=255) cGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGCTGCAGAAGCTGAAAATGATGTCTCCg > 1:649824/1‑59 (MQ=255) cGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGCTGCAGAAGCTGAAAATGATGTCTCCg > 1:958979/1‑59 (MQ=255) cGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGCTGCAGAAGCTGAAAATGATGTCTCCg > 1:1134111/1‑59 (MQ=255) cGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGCTGCAGAAGCTGAAAATGATGTCTCCg > 1:3053286/1‑59 (MQ=255) cGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGCTGCAGAAGCTGAAAATGATGTCTCCg > 1:2963053/1‑59 (MQ=255) cGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGCTGCAGAAGCTGAAAATGATGTCTCCg > 1:1657013/1‑59 (MQ=255) cGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGCTGCAGAAGCTGAAAATGATGTCTCCg > 1:25864/1‑59 (MQ=255) cGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGCTGCAGAAGCTGAAAATGATGTCTCCg > 1:2567893/1‑59 (MQ=255) cGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGCTGCAGAAGCTGAAAATGATGTCTCCg > 1:1817595/1‑59 (MQ=255) cGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGCTGCAGAAGCTGAAAATGATGTCTCCg > 1:1822/1‑59 (MQ=255) cGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGCTGCAGAAGCTGAAAATGATGTCTCCg > 1:1473870/1‑59 (MQ=255) cGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGCTGCAGAAGCTGAAAATGATGTCTCCg > 1:1937704/1‑59 (MQ=255) agagGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAatgat < 1:565670/48‑1 (MQ=255) agagGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAATGATGTCTCCGATGTCGGCAGAAGc > 1:2933040/1‑69 (MQ=255) agagGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAATGATGTCTCCGATGTCGGCAGAAGc > 1:1702604/1‑69 (MQ=255) agagGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAATGATGTCTCCGATGTCGGCAGAAGc > 1:3409728/1‑69 (MQ=255) agagGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAATGATGTCTCCGATGTCGGCAGAAGc > 1:2121303/1‑69 (MQ=255) agagGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAATGATGTCTCCGATGTCGGCAGAAGc > 1:1210031/1‑69 (MQ=255) agagGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAATGATGTCTCCGATGTCGGCAGAAGc > 1:2259604/1‑69 (MQ=255) agagGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAATGATGTCTCCGATGTCGGCAGAAGc > 1:119906/1‑69 (MQ=255) gCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGGTGAAAATGATGTCTCCGATGTCGGCAGAAGCGAc < 1:738161/68‑1 (MQ=255) aaaaGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAATGATGTCTCCGATGTCGGCAGAAGCGACCGt < 1:1377719/69‑1 (MQ=255) aaaaGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAATGATGTCTCCGATGTCGGCAGAAGCGACCGt < 1:289283/69‑1 (MQ=255) aaaaGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAATGATGTCTCCGATGTCGGCACAAGCGACCGt < 1:477169/69‑1 (MQ=255) aaaGAGAAAGCGGAAGCAGAGTTTCAGAAGCTGAAAATGATGTCTCCGATGTCGGCAGAAGCGACCGt > 1:1414329/1‑68 (MQ=255) aaaGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAatgat < 1:3015154/41‑1 (MQ=255) aaaGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAATGATGTCTCCGATGTCGGCAGAAGCGACCGTa > 1:1746847/1‑69 (MQ=255) aaaGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAATGATGTCTCCGATGTCGGCAGAAGCGACCGTa > 1:1557228/1‑69 (MQ=255) ggAAGCAGAGGTGCAGAAGCTGAAAATGATGTCTCCGATGTCGGCAGAAGCGACCGTAGTGCGTGGTta < 1:1452149/69‑1 (MQ=255) ttGCAGAAGCTGAAAATGATGTCTCCGATGTCGGCAGAAGCGACCGt < 1:2834976/47‑1 (MQ=255) ttGCAGAAGCTGAAAATGATGTCTCCGATGTCGGCAGAAGCGACCGTAGTGCGTGGTTATATCGACTgg > 1:1545803/1‑69 (MQ=255) ttGCAGAAGCTGAAAATGATGTCTCCGATGTCGGCAGAAGCGACCGTAGTGCGTGGTTATATCGACTgg > 1:1932568/1‑69 (MQ=255) ttGCAGAAGCTGAAAATGATGTCTCCGATGTCGGCAGAAGCGACCGTAGTGCGTGGTTATATCGACTgg > 1:2085531/1‑69 (MQ=255) ttGCAGAAGCTGAAAATGATGTCTCCGATGTCGGCAGAAGCGACCGTAGTGCGTGGTTATATCGACTgg > 1:2121131/1‑69 (MQ=255) ttGCAGAAGCTGAAAATGATGTCTCCGATGTCGGCAGAAGCGACCGTAGTGCGTGGTTATATCGACTgg > 1:3219728/1‑69 (MQ=255) ttGCAGAAGCTGAAAATGATGTCTCCGATGTCGGCAGAAGCGACCGTAGTGCGTGGTTATATCGACTgg > 1:2225034/1‑69 (MQ=255) ttGCAGAAGCTGAAAATGATGTCTCCGATGTCGGCAGAAGCGACCGTAGTGCGTGGTTATATCGACTgg > 1:1038489/1‑69 (MQ=255) ttGCAGAAGCTGAAAATGATGTCTCCGATGTCGGCAGAAGCGACCGTAGTGCGTGGTTATATCGACTgg > 1:978333/1‑69 (MQ=255) | GCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAATGATGTCTCCGATGTCGGCAGAAGCGACCGTAGTGCGTGGTTATATCGACTGG > minE/288113‑288247 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |