| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 288,355 | A→T | 52.3% | R333R (CGA→CGT) | lon → | DNA‑binding ATP‑dependent protease La |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 288,355 | 0 | A | T | 52.3% | 5.8 / 103.0 | 86 | R333R (CGA→CGT) | lon | DNA‑binding ATP‑dependent protease La |
| Reads supporting (aligned to +/- strand): ref base A (26/15); new base T (35/10); total (61/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.61e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01 | |||||||||||
AAAGACCTGCGTCAGGCGCAGG‑AAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTCAACAAAATCAAGGGACCGATCCTCTGCCTGGT > minE/288287‑288420 | aaaGACCTGCGTCAGGCGCAGG‑AAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGa > 1:379465/1‑69 (MQ=255)aaaGACCTGCGTCAGGCGCAGG‑AAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGa > 1:227321/1‑69 (MQ=255)aaaGACCTGCGTCAGGCGCAGG‑AAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGa > 1:422360/1‑69 (MQ=255)aaaGACCTGCGTCAGGCGCAGG‑AAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGa > 1:1920945/1‑69 (MQ=255)aaaGACCTGCGTCAGGCGCAGG‑AAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGa > 1:1906999/1‑69 (MQ=255) aaGACCTGCGTCAGGCGCAGGAAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGa < 1:2092344/69‑1 (MQ=255) aaGACCTGCGTCAGGCGCAGGAAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGa < 1:1320272/69‑1 (MQ=255) aaGACCTGCGTCAGGCGCAGGAAAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGa < 1:765724/69‑1 (MQ=255) aGACCTGCGTCAGGCGCAGG‑AAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAAt > 1:1695938/1‑69 (MQ=255) aGACCTGCGTCAGGCGCAGG‑AAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAAt > 1:176343/1‑69 (MQ=255) aGACCTGCGTCAGGCGCAGG‑AAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAAt > 1:827561/1‑69 (MQ=255) aGACCTGCGTCAGGCGCAGG‑AAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAAt > 1:287020/1‑69 (MQ=255) aGGCGCAGG‑AAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGTATCCTTGAGTATc > 1:246437/1‑69 (MQ=255) aGGCGCAGG‑AAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGTATCCTTGAGTATc > 1:3402473/1‑69 (MQ=255) aGGCGCAGG‑AAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGTATCCTTGAGTATc > 1:1763451/1‑69 (MQ=255) aGGCGCAGG‑AAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGTATCCTTGAGTATc > 1:2218836/1‑69 (MQ=255) aGGCGCAGG‑AAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGTATCCTTGAGTATc > 1:3032731/1‑69 (MQ=255) aGGCGCAGG‑AAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGTATCCTTGAGTATc > 1:2556360/1‑69 (MQ=255) aGGCGCAGG‑AAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGTATCCTTGAGTATc > 1:166384/1‑69 (MQ=255) aGGCGCAGG‑AAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGTATCCTTGAGTATc > 1:2735364/1‑69 (MQ=255) aGGCGCAGG‑AAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGTATCCTTGAGTATc > 1:3360137/1‑69 (MQ=255) aGGCGCAGG‑AAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGTATCCTTGAGTATc > 1:72534/1‑69 (MQ=255) aGGCGCAGG‑AAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGTATCCTTGAGTATc > 1:2753001/1‑69 (MQ=255) aGGCGCAGG‑AAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGTATCCTTGAGTATc > 1:2799501/1‑69 (MQ=255) aGGCGCAGG‑AAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGTATCCTTGAGTATc > 1:288591/1‑69 (MQ=255) aGGCGCAGG‑AAATCCTTGATACCGACCATTATGGTCTGGAGCGAGTGAAAGATCGTATCCTTGAGTATc > 1:645854/1‑69 (MQ=255) aaTCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGt < 1:695914/66‑1 (MQ=255) aaTCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGt > 1:1861944/1‑66 (MQ=255) aaTCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGt < 1:872288/66‑1 (MQ=255) cTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGc > 1:49694/1‑69 (MQ=255) cTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGc > 1:1782959/1‑69 (MQ=255) cTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGc > 1:1089936/1‑69 (MQ=255) aTACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGt < 1:2288204/58‑1 (MQ=255) ccGACCATTATGGTCTGGAGCGCGTGAAAGATCGTATCCTTGAGTATc > 1:2921998/1‑48 (MQ=255) ccGACCATTATGGTCTGGAGCGCGTGAAAGATCGTATCCTTGAGTATCt > 1:6317/1‑49 (MQ=255) ccGACCATTATGGTCTGGAGCGCGTGAAAGATCGTATCCTTGAGTATCt > 1:490349/1‑49 (MQ=255) ccGACCATTATGGTCTGGAGCGCGTGAAAGATCGTATCCTTGAGTATCt > 1:3239437/1‑49 (MQ=255) ccGACCATTATGGTCTGGAGCGCGTGAAAGATCGTATCCTTGAGTATCt > 1:1122115/1‑49 (MQ=255) ccGACCATTATGGTCTGGAGCGCGTGAAAGATCGTATCCTTGAGTATCt > 1:1135233/1‑49 (MQ=255) ccGACCATTATGGTCTGGAGCGCGTGAAAGATCGTATCCTTGAGTATCt > 1:2845224/1‑49 (MQ=255) ccGACCATTATGGTCTGGAGCGCGTGAAAGATCGTATCCTTGAGTATCt > 1:1173955/1‑49 (MQ=255) ccGACCATTATGGTCTGGAGCGCGTGAAAGATCGTATCCTTGAGTATCt > 1:3302910/1‑49 (MQ=255) ccGACCATTATGGTCTGGAGCGCGTGAAAGATCGTATCCTTGAGTATCt > 1:2773202/1‑49 (MQ=255) ccGACCATTATGGTCTGGAGCGCGTGAAAGATCGTATCCTTGAGTATCt > 1:921320/1‑49 (MQ=255) ccGACCATTATGGTCTGGAGCGCGTGAAAGATCGTATCCTTGAGTATCt > 1:1519169/1‑49 (MQ=255) ccGACCATTATGGTCTGGAGCGCGTGAAAGATCGTATCCTTGAGTATCt > 1:1639692/1‑49 (MQ=255) ccGACCATTATGGTCTGGAGCGCGTGAAAGATCGTATCCTTGAGTATCt > 1:2200762/1‑49 (MQ=255) ccGACCATTATGGTCTGGAGCGCGTGAAAGATCGTATCCTTGAGTATCt > 1:2102119/1‑49 (MQ=255) ccGACCATTATGGTCTGGAGCGCGTGAAAGATCGTATCCTTGAGTATCt > 1:1873795/1‑49 (MQ=255) ccGACCATTATGGTCTGGAGCGCGTGAAAGATCGTATCCTTGAGTATCt > 1:2416497/1‑49 (MQ=255) ccGACCATTATGGTCTGGAGCGCGTGAAAGATCGTATCCTTGAGTATCt > 1:2399427/1‑49 (MQ=255) ccGACCATTATGGTCTGGAGCGCGTGAAAGATCGTATCCTTGAGTATCt > 1:1962142/1‑49 (MQ=255) ccGACCATTATGGTCTGGAGCGCGTGAAAGATCGTATCCTTGAGTATCt > 1:425256/1‑49 (MQ=255) ccGACCA‑TATGGTCTGGAGCGCGTGAAAGATCGTATCCTTGAGTATCt > 1:1805446/1‑48 (MQ=38) aTGGTCTGGAGCGCGTGAAAGATCGTATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTcaaca < 1:488299/63‑1 (MQ=255) aTGGTCTGGAGCGCGTGAAAGATCGTATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTcaaca < 1:599496/63‑1 (MQ=255) aTGGTCTGGAGCGCGTGAAAGATCGTATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTcaaca < 1:981921/63‑1 (MQ=255) aTGGTCTGGAGCGCGTGAAAGATCGTATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTcaaca < 1:1198145/63‑1 (MQ=255) aTGGTCTGGAGCGCGTGAAAGATCGTATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTcaaca < 1:2060755/63‑1 (MQ=255) aTGGTCTGGAGCGCGTGAAAGATCGTATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTcaaca < 1:2492575/63‑1 (MQ=255) aTGGTCTGGAGCGCGTGAAAGATCGTATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTcaaca < 1:2382464/63‑1 (MQ=255) aTGGTCTGGAGCGCGTGAAAGATCGTATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTcaaca < 1:2396134/63‑1 (MQ=255) aTGGTCTGGAGCGCGTGAAAGATCGTATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTcaaca < 1:2464619/63‑1 (MQ=255) aTGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTCAACAAAATCa > 1:2957980/1‑69 (MQ=255) aTGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTCAACAAAATCa > 1:2913720/1‑69 (MQ=255) aTGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTCAACAAAATCa > 1:634761/1‑69 (MQ=255) aTGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTCAACAAAATCa > 1:490460/1‑69 (MQ=255) aTGGTCTCGAGCGCGTGAAAGATCGAATCCTTGAGTATCtt > 1:3221906/1‑41 (MQ=255) aTGGTATGGAGCGCGTGAAAGATCGTATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTcaaca < 1:1697272/63‑1 (MQ=255) ggTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTCAACAAAATCaa < 1:2178763/68‑1 (MQ=255) cTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTCAACAAAATCAAGGGa < 1:626408/69‑1 (MQ=255) cTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTCAACAAAATCAAGGGa < 1:341666/69‑1 (MQ=255) cTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTCAACAAAATCAAGGGa < 1:879155/69‑1 (MQ=255) cTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTCAACAAAATCAAGGGa < 1:973496/69‑1 (MQ=255) tGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTCAACAAAATCAAGGGa < 1:779775/59‑1 (MQ=255) tGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTCAACAAAATCAAGGGACCGATCctct < 1:1393086/69‑1 (MQ=255) tCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTcaacaa > 1:1409069/1‑42 (MQ=255) tCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTcaacaa > 1:1505655/1‑42 (MQ=255) tCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTcaacaa > 1:1668642/1‑42 (MQ=255) tCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTcaacaa > 1:1233631/1‑42 (MQ=255) tCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTcaacaa > 1:2952653/1‑42 (MQ=255) tCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTcaacaa > 1:2031104/1‑42 (MQ=255) tCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTcaacaa > 1:509836/1‑42 (MQ=255) tCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTcaacaa > 1:49376/1‑42 (MQ=255) tCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTcaacaa > 1:2371414/1‑42 (MQ=255) tCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTcaacaa > 1:368780/1‑42 (MQ=255) tCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTcaacaa > 1:3306505/1‑42 (MQ=255) tCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTcaacaa > 1:2785244/1‑42 (MQ=255) tCGAATCCTTGAGTATATTGCGGTTCAAAGCCGTGTcaacaa > 1:3129813/1‑42 (MQ=255) cGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTCAACAAAATCAAGGGACCGATCCTCTGCCTGGt < 1:1545833/68‑1 (MQ=255) cGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTCAACAAAATCAAGGGACCGATCCTCTGCCTGGt < 1:2743996/68‑1 (MQ=255) cGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTCAACAAAATCAAGGGACCGATCCTCTGCCTGGt < 1:3348632/68‑1 (MQ=255) cGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTCAACAAAATCAAGGGACCGATCCTCTGCCTGGt < 1:3324118/68‑1 (MQ=255) cGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTCAACAAAATCAAGGGACCGATCCTCTGCCTGGt < 1:1104477/68‑1 (MQ=255) | AAAGACCTGCGTCAGGCGCAGG‑AAATCCTTGATACCGACCATTATGGTCTGGAGCGCGTGAAAGATCGAATCCTTGAGTATCTTGCGGTTCAAAGCCGTGTCAACAAAATCAAGGGACCGATCCTCTGCCTGGT > minE/288287‑288420 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |