| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 289,285 | G→A | 26.1% | T643T (ACG→ACA) | lon → | DNA‑binding ATP‑dependent protease La |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 289,285 | 0 | G | A | 26.1% | 70.2 / 20.4 | 61 | T643T (ACG→ACA) | lon | DNA‑binding ATP‑dependent protease La |
| Reads supporting (aligned to +/- strand): ref base G (25/20); new base A (8/8); total (33/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.75e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.76e-01 | |||||||||||
AAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCA > minE/289218‑289350 | aaGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACgg > 1:836847/1‑69 (MQ=255)aaGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACgg > 1:399938/1‑69 (MQ=255) aCCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTcgtgcg > 1:2428942/1‑69 (MQ=255) aCCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTcgtgcg > 1:2834026/1‑69 (MQ=255) aCCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTcgtgcg > 1:821351/1‑69 (MQ=255) aCCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTcgtgc > 1:905907/1‑68 (MQ=255) aCCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTcgtgc > 1:2478921/1‑68 (MQ=255) aCCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTcgtgc > 1:2845227/1‑68 (MQ=255) cTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTcgtgcg < 1:2735318/67‑1 (MQ=255) cTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTcgtgcg < 1:1243243/67‑1 (MQ=255) cTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTcgtgcg < 1:2322496/67‑1 (MQ=255) cTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTcgtgcg < 1:2200297/67‑1 (MQ=255) cTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTcgtgcg < 1:310870/67‑1 (MQ=255) taCCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTcgtgcgcgtg > 1:2225484/1‑68 (MQ=255) ccGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTcgtgcgcgtgcg > 1:166754/1‑68 (MQ=255) ccGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTcgtgcgcgtgc > 1:2027336/1‑67 (MQ=255) gAAGTGATGCAGGAGTCTATTCAGGCGGCGTTAACAGTGGTTCGTGCGCGTGCGGAAAAACTggg > 1:2077347/1‑65 (MQ=255) gAAGTGATGCAGGAGTCTATTCAGGCGGCGTTAACAGTGGTTCGTGCGCGTGCGGAAAAACTggg > 1:3329285/1‑65 (MQ=255) gAAGTGATGCAGGAGTCTATTCAGGCGGCGTTAACAGTGGTTCGTGCGCGTGCGGAAAAACTggg > 1:2939539/1‑65 (MQ=255) gAAGTGATGCAGGAGTCTATTCAGGCGGCGTTAACAGTGGTTCGTGCGCGTGCGGAAAAACTggg > 1:764517/1‑65 (MQ=255) gAAGTGATGCAGGAGTCTATTCAGGCGGCGTTAACAGTGGTTCGTGCGCGTGCGGAAAAACTggg > 1:546090/1‑65 (MQ=255) gAAGTGATGCAGGAGTCTATTCAGGCGGCGTTAACAGTGGTTCGTGCGCGTGCGGAAAAACTggg > 1:880730/1‑65 (MQ=255) gAAGTGATGCAGGAGTCTATTCAGGCGGCGTTAACAGTGGTTCGTGCGCGTGCGGAAAAACTggg > 1:1670946/1‑65 (MQ=255) gAAGTGATGCAGGAGTCTATTCAGGCGGCGTTAACAGTGGTTCGTGCGCGTGCGGAAAAACTggg > 1:1148358/1‑65 (MQ=255) gATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAAcc > 1:732517/1‑68 (MQ=255) aTGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCt > 1:3241194/1‑69 (MQ=255) aTGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCt > 1:3382738/1‑69 (MQ=255) aTGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCt > 1:27390/1‑69 (MQ=255) aTGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCt > 1:1551901/1‑69 (MQ=255) gTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGAttttt > 1:1534370/1‑68 (MQ=255) gTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGAttttt > 1:2201664/1‑68 (MQ=255) gcggcgTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACGAAAAACGt < 1:1141961/69‑1 (MQ=255) gcggcgTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACGAAAAACGt < 1:2770340/69‑1 (MQ=255) gcggcgTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACGAAAAACGt < 1:782128/69‑1 (MQ=255) gcggcgTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACGAAAAACGt < 1:458002/69‑1 (MQ=255) gcggcgTTAACAGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACGAAAAACGt < 1:183888/69‑1 (MQ=255) gcggcgTTAACAGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACGAAAAACGt < 1:1364912/69‑1 (MQ=255) gcggcgTTAACAGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACGAAAAACGt < 1:1525682/69‑1 (MQ=255) gcggcgTTAACAGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACGAAAAACGt < 1:3420420/69‑1 (MQ=255) gcggcgTTAACAGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACGAAAAACGt < 1:2108357/69‑1 (MQ=255) gcggcgTTAACAGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACGAAAAACGt < 1:1042109/69‑1 (MQ=255) gcggcgTTAACAGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACGAAAAACGt < 1:233016/69‑1 (MQ=255) gcggcgTTAACAGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACGAAAAACGt < 1:3097220/69‑1 (MQ=255) cggcgTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACGAAAAACGt < 1:3182216/68‑1 (MQ=255) cggcgTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACGAAAAACGTg < 1:670724/69‑1 (MQ=255) cggcgTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACGAAAAACGTg < 1:1465356/69‑1 (MQ=255) cggcgTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACGAAAAACGTg < 1:757650/69‑1 (MQ=255) cggcgTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACGAAAAACGTg < 1:744014/69‑1 (MQ=255) cggcgTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACGAAAAACGTg < 1:719500/69‑1 (MQ=255) cggcgTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACGAAAAACGTg < 1:3155109/69‑1 (MQ=255) cggcgTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACGAAAAACGTg < 1:572252/69‑1 (MQ=255) cggcgTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACGAAAAACGTg < 1:1783958/69‑1 (MQ=255) aaCGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACGAAAAACGTGCCAtcca > 1:2198268/1‑69 (MQ=255) aaCGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACGAAAAACGTGACAtcca > 1:1733049/1‑69 (MQ=255) aaCGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACGAAAAACGTGACAtcca > 1:730344/1‑69 (MQ=255) aaCGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACGAAAAACGTGACAtcca > 1:2675253/1‑69 (MQ=255) aaCGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACGAAAAACGTGACAtcca > 1:3123679/1‑69 (MQ=255) aaCGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACGAAAAACGTGACAtcca < 1:2378701/69‑1 (MQ=255) aaCGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACGAAAAACGTGACAtcca > 1:280676/1‑69 (MQ=255) aaCGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACGAAAAACGTGACAtcca > 1:2195499/1‑69 (MQ=255) aaCGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACGAAAAACGTGACAtcca > 1:3189261/1‑69 (MQ=255) aaCGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACGAAAAACGTGACAtcca > 1:244045/1‑69 (MQ=255) aaCGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACGAAAAACGTGACAtcca < 1:2694300/69‑1 (MQ=255) | AAGGCAAACTGACCTATACCGGTTCGCTCGGCGAAGTGATGCAGGAGTCCATTCAGGCGGCGTTAACGGTGGTTCGTGCGCGTGCGGAAAAACTGGGGATCAACCCTGATTTTTACGAAAAACGTGACATCCA > minE/289218‑289350 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |