Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 299,920 C→T 51.7% G272G (GGC→GGT mdlB → fused predicted multidrug transporter subunits and ATP binding components of ABC superfamily

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE299,9200CT51.7% ‑0.3 / 138.7 149G272G (GGC→GGTmdlBfused predicted multidrug transporter subunits and ATP binding components of ABC superfamily
Reads supporting (aligned to +/- strand):  ref base C (30/42);  new base T (27/50);  total (57/92)
Fisher's exact test for biased strand distribution p-value = 5.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.37e-01

Only 100 of 155 total aligned reads displayed.

TTTCTGCTGCGTCCGCTGCTGAGTCTGTTTTCATCGCTCATTCTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTA  >  minE/299852‑299987
                                                                    |                                                                   
tttCTGCTGCGTCCGCTGCTGAGTCTGTTTTCATCGCTCATTCTTTGTGGCTTGTTGATGCTGTTTGGc                                                                     <  1:941165/69‑1 (MQ=255)
tttCTGCTGCGTCCGCTGCTGAGTCTGTTTTCATCGCTCATTCTTTGTGGCTTGTTGATGCTGTTTGGc                                                                     <  1:1963986/69‑1 (MQ=255)
tttCTGCTGCGTCCGCTGCTGAGTCTGTTTTCATCGCTCATTCTTTGTGGCTTGTTGATGCTGTTTGGc                                                                     <  1:2161760/69‑1 (MQ=255)
tttCTGCTGCGTCCGCTGCTGAGTCTGTTTTCATCGCTCATTCTTTGTGGCTTGTTGATGCTGTTTGGc                                                                     <  1:491835/69‑1 (MQ=255)
 ttCTGCTGCGTCCGCTGCTGAGTCTGTTTTCATCGCTCATTCTTTGTGGCTTGTTGATGCTGTTTGGc                                                                     <  1:2421322/68‑1 (MQ=255)
    tgctgcGTCCGCTGCTGAGTCTGTTTTCATCGCTCATTCTTTGTGGCTTGTTGATGCTGTTTGGCTtc                                                                  <  1:1045911/68‑1 (MQ=255)
           tCCGCTGCTGAGTCTGTTTTCATCGCTCATTCTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAg                                                          >  1:893132/1‑69 (MQ=255)
           tCCGCTGCTGAGTCTGTTTTCATCGCTCATTCTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAg                                                          >  1:620323/1‑69 (MQ=255)
           tCCGCTGCTGAGTCTGTTTTCATCGCTCATTCTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAg                                                          >  1:1583284/1‑69 (MQ=255)
                 gctgAGTCTGTTTTCATCGCTCATTCTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCAc                                                    <  1:2546919/69‑1 (MQ=255)
                 gctgAGTCTGTTTTCATCGCTCATTCTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCAc                                                    <  1:1500144/69‑1 (MQ=255)
                 gctgAGTCTGTTTTCATCGCTCATTCTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCAc                                                    <  1:3401633/69‑1 (MQ=255)
                 gctgAGTCTGTTTTCATCGCTCATTCTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCAc                                                    <  1:2669733/69‑1 (MQ=255)
                 gctgAGTCTGTTTTCATCGCTCATTCTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCAc                                                    <  1:722044/69‑1 (MQ=255)
                 gctgAGTCTGTTTTCATCGCTCATTCTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCAc                                                    <  1:953047/69‑1 (MQ=255)
                       tCTGTTTTCATCGCTCATTCTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCACCATTGa                                              <  1:2516698/69‑1 (MQ=255)
                       tCTGTTTTCATCGCTCATTCTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCACCATTGa                                              <  1:981078/69‑1 (MQ=255)
                       tCTGTTTTCATCGCTCATTCTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCACCATTGa                                              <  1:3307884/69‑1 (MQ=255)
                        cTGTTTTCATCGCTCATTCTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCACCATTGa                                              <  1:3309813/68‑1 (MQ=255)
                             ttCATCGCTCATTCTTTGTGGCTTGTTGATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAg                                            <  1:1487083/65‑1 (MQ=255)
                             ttCATCGCTCATTCTTTGTGGCTTGTTGATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAg                                            <  1:407058/65‑1 (MQ=255)
                             ttCATCGCTCATTCTTTGTGGCTTGTTGATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAg                                            <  1:2146353/65‑1 (MQ=255)
                             ttCATCGCTCATTCTTTGTGGCTTGTTGATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAg                                            <  1:283782/65‑1 (MQ=255)
                             ttCATCGCTCATTCTTTGTGGCTTGTTGATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAg                                            <  1:2174845/65‑1 (MQ=255)
                             ttCATCGCTCATTCTTTGTGGCTTGTTGATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAg                                            <  1:1726761/65‑1 (MQ=255)
                             ttCATCGCTCATTCTTTGTGGCTTGTTGATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAg                                            <  1:2771734/65‑1 (MQ=255)
                             ttCATCGCTCATTCTTTGTGGCTTGTTGATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAg                                            <  1:2305613/65‑1 (MQ=255)
                             ttCATCGCTCATTCTTTGTGGCTTGTTGATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAg                                            <  1:2669136/65‑1 (MQ=255)
                             ttCATCGCTCATTCTTTGTGGCTTGTTGATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAg                                            <  1:228625/65‑1 (MQ=255)
                             ttCATCGCTCATTCTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGc                                                             >  1:2305282/1‑48 (MQ=255)
                             ttCATCGCTCATTCTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGc                                                             >  1:1833018/1‑48 (MQ=255)
                             ttCATCGCTCATTCTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGc                                                             >  1:3153787/1‑48 (MQ=255)
                             ttCATCGCTCATTCTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGc                                                             >  1:493502/1‑48 (MQ=255)
                             ttCATCGCTCATTCTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGc                                                             >  1:511134/1‑48 (MQ=255)
                             ttCATCGCTCATTCTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGc                                                             >  1:519755/1‑48 (MQ=255)
                                     tCATTCTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGCTg                                 <  1:2803189/68‑1 (MQ=255)
                                     tCATTCTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGCTg                                 <  1:2501615/68‑1 (MQ=255)
                                     tCATTCTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGCTg                                 <  1:2249103/68‑1 (MQ=255)
                                     tCATTCTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGCTg                                 <  1:651853/68‑1 (MQ=255)
                                     tCATTCTTTGTGGCATGTTGATGCTGTTTGGCTTCTCCGcc                                                            >  1:133772/1‑41 (MQ=255)
                                          cTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGCTGTATGCg                           >  1:2546801/1‑69 (MQ=255)
                                          cTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGCTGTATGCg                           >  1:3134891/1‑69 (MQ=255)
                                          cTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGCTGTATGCg                           >  1:2525491/1‑69 (MQ=255)
                                          cTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGCTGTATGCg                           >  1:1476388/1‑69 (MQ=255)
                                          cTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGCTGTATGCg                           >  1:1314394/1‑69 (MQ=255)
                                          cTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGCTGTATGCg                           >  1:1330677/1‑69 (MQ=255)
                                          cTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCACCATCGAAGTGGGCGTGCTGTATGCg                           >  1:885830/1‑69 (MQ=255)
                                             tgtgGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCACCATTGAAGTGGGCg                                      >  1:3118213/1‑55 (MQ=255)
                                                    tgttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTTGGCGTGTTg                                 <  1:2710836/53‑1 (MQ=255)
                                                    tgttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTg                                 <  1:3186486/53‑1 (MQ=255)
                                                    tgttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTg                                 <  1:677320/53‑1 (MQ=255)
                                                    tgttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTg                                 <  1:1253067/53‑1 (MQ=255)
                                                    tgttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTg                                 <  1:305668/53‑1 (MQ=255)
                                                    tgttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTg                                 <  1:3186312/53‑1 (MQ=255)
                                                    tgttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTg                                 <  1:1727269/53‑1 (MQ=255)
                                                    tgttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTg                                 <  1:1392302/53‑1 (MQ=255)
                                                    tgttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTg                                 <  1:329310/53‑1 (MQ=255)
                                                    tgttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTg                                 <  1:1552178/53‑1 (MQ=255)
                                                    tgttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTg                                 <  1:151435/53‑1 (MQ=255)
                                                    tgttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTg                                 <  1:1399998/53‑1 (MQ=255)
                                                    tgttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTg                                 <  1:1449379/53‑1 (MQ=255)
                                                    tgttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTg                                 <  1:1076399/53‑1 (MQ=255)
                                                    tgttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTg                                 <  1:1781872/53‑1 (MQ=255)
                                                    tgttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTg                                 <  1:1782553/53‑1 (MQ=255)
                                                    tgttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTg                                 <  1:705815/53‑1 (MQ=255)
                                                    tgttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTg                                 <  1:2386779/53‑1 (MQ=255)
                                                    tgttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTg                                 <  1:962722/53‑1 (MQ=255)
                                                    tgttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTg                                 <  1:2020991/53‑1 (MQ=255)
                                                    tgttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTg                                 <  1:2072265/53‑1 (MQ=255)
                                                    tgttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTg                                 <  1:2079711/53‑1 (MQ=255)
                                                    tgttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTg                                 <  1:2087186/53‑1 (MQ=255)
                                                    tgttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTg                                 <  1:1107808/53‑1 (MQ=255)
                                                     gttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGa                                              >  1:2664021/1‑39 (MQ=255)
                                                     gttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTg                                 >  1:1263428/1‑52 (MQ=255)
                                                     gttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTGTATGCGTTCATCAGTTa                >  1:113362/1‑69 (MQ=255)
                                                     gttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTGTATGCGTTCATCAGTTa                >  1:686490/1‑69 (MQ=255)
                                                     gttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTGTATGCGTTCATCAGTTa                >  1:719243/1‑69 (MQ=255)
                                                     gttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTGTATGCGTTCATCAGTTa                >  1:2364986/1‑69 (MQ=255)
                                                     gttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTGTATGCGTTCATCAGTTa                >  1:2650539/1‑69 (MQ=255)
                                                     gttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTGTATGCGTTCATCAGTTa                >  1:2277330/1‑69 (MQ=255)
                                                     gttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTGTATGCGTTCATCAGTTa                >  1:2461122/1‑69 (MQ=255)
                                                     gttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTGTATGCGTTCATCAGTTa                >  1:2628071/1‑69 (MQ=255)
                                                     gttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTGTATGCGTTCATCAGTTa                >  1:363833/1‑69 (MQ=255)
                                                     gttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTGTATGCGTTCATCAGTTa                >  1:1646220/1‑69 (MQ=255)
                                                     gttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTGTATGCGTTCATCAGTTa                >  1:2835637/1‑69 (MQ=255)
                                                     gttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTGTATGCGTTCATCAGTTa                >  1:1604199/1‑69 (MQ=255)
                                                     gttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTGTATGCGTTCATCAGTTa                >  1:3258881/1‑69 (MQ=255)
                                                     gttgATGCTGTTTGGTTTCTCCGCCAGCGGCACAATTGAAGTgg                                         >  1:704423/1‑44 (MQ=38)
                                                     gttgATGCTGTTTGGTTTCTCCGCCAGCGGCAACATTGAAGTGGGCGTGTTGTATGCGTTCATCAGTTa                >  1:118212/1‑69 (MQ=255)
                                                      ttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTg                                 <  1:2648460/51‑1 (MQ=255)
                                                                   gCTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACtt   <  1:1643052/68‑1 (MQ=255)
                                                                   gCTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACtt   <  1:1572628/68‑1 (MQ=255)
                                                                   gCTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACtt   <  1:845832/68‑1 (MQ=255)
                                                                   gCTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACtt   <  1:3290266/68‑1 (MQ=255)
                                                                   gCTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACtt   <  1:1896420/68‑1 (MQ=255)
                                                                   gCTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACtt   <  1:3417329/68‑1 (MQ=255)
                                                                   gCTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACtt   <  1:2144503/68‑1 (MQ=255)
                                                                   gCTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTa  >  1:1189131/1‑69 (MQ=255)
                                                                   gCTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTa  >  1:972547/1‑69 (MQ=255)
                                                                   gCTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTa  >  1:470430/1‑69 (MQ=255)
                                                                    |                                                                   
TTTCTGCTGCGTCCGCTGCTGAGTCTGTTTTCATCGCTCATTCTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTA  >  minE/299852‑299987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: