| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 299,954 | C→T | 52.8% | L284L (CTG→TTG) | mdlB → | fused predicted multidrug transporter subunits and ATP binding components of ABC superfamily |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 299,954 | 0 | C | T | 52.8% | 7.7 / 109.6 | 123 | L284L (CTG→TTG) | mdlB | fused predicted multidrug transporter subunits and ATP binding components of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base C (26/32); new base T (33/32); total (59/64) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.89e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 124 total aligned reads displayed.
TCATTCTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGC > minE/299889‑300021 | tCATTCTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGCTg < 1:1374686/68‑1 (MQ=255)tCATTCTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGCTg < 1:2249103/68‑1 (MQ=255)tCATTCTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGCTg < 1:651853/68‑1 (MQ=255)tCATTCTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGCTg < 1:2803189/68‑1 (MQ=255)tCATTCTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGCTg < 1:2501615/68‑1 (MQ=255) cTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGCTGTATGCg > 1:1476388/1‑69 (MQ=255) cTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGCTGTATGCg > 1:1314394/1‑69 (MQ=255) cTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGCTGTATGCg > 1:2271323/1‑69 (MQ=255) cTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGCTGTATGCg > 1:2546801/1‑69 (MQ=255) cTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGCTGTATGCg > 1:2814435/1‑69 (MQ=255) cTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGCTGTATGCg > 1:1646954/1‑69 (MQ=255) cTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCACCATCGAAGTGGGCGTGCTGTATGCg > 1:885830/1‑69 (MQ=255) tgttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTTGGCGTGTTg < 1:2710836/53‑1 (MQ=255) tgttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTg < 1:1727269/53‑1 (MQ=255) tgttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTg < 1:962722/53‑1 (MQ=255) tgttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTg < 1:1781872/53‑1 (MQ=255) tgttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTg < 1:1782553/53‑1 (MQ=255) tgttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTg < 1:1572769/53‑1 (MQ=255) tgttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTg < 1:359403/53‑1 (MQ=255) tgttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTg < 1:2020991/53‑1 (MQ=255) tgttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTg < 1:2072265/53‑1 (MQ=255) tgttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTg < 1:2079711/53‑1 (MQ=255) tgttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTg < 1:2386779/53‑1 (MQ=255) tgttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTg < 1:3186486/53‑1 (MQ=255) tgttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTg < 1:3186312/53‑1 (MQ=255) tgttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTg < 1:305668/53‑1 (MQ=255) tgttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTg < 1:1076399/53‑1 (MQ=255) tgttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTg < 1:705815/53‑1 (MQ=255) tgttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTg < 1:933411/53‑1 (MQ=255) tgttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTg < 1:1392302/53‑1 (MQ=255) tgttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTg < 1:1399998/53‑1 (MQ=255) tgttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTg < 1:1107808/53‑1 (MQ=255) tgttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTg < 1:1449379/53‑1 (MQ=255) tgttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTg < 1:1480764/53‑1 (MQ=255) tgttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTg < 1:677320/53‑1 (MQ=255) tgttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTg < 1:151435/53‑1 (MQ=255) tgttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTg < 1:1552178/53‑1 (MQ=255) gttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTg > 1:1263428/1‑52 (MQ=255) gttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTGTATGCGTTCATCAGTTa > 1:2461122/1‑69 (MQ=255) gttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTGTATGCGTTCATCAGTTa > 1:2650539/1‑69 (MQ=255) gttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTGTATGCGTTCATCAGTTa > 1:3299975/1‑69 (MQ=255) gttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTGTATGCGTTCATCAGTTa > 1:2692560/1‑69 (MQ=255) gttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTGTATGCGTTCATCAGTTa > 1:2295330/1‑69 (MQ=255) gttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTGTATGCGTTCATCAGTTa > 1:2277330/1‑69 (MQ=255) gttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTGTATGCGTTCATCAGTTa > 1:1242445/1‑69 (MQ=255) gttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTGTATGCGTTCATCAGTTa > 1:3258881/1‑69 (MQ=255) gttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTGTATGCGTTCATCAGTTa > 1:2628071/1‑69 (MQ=255) gttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTGTATGCGTTCATCAGTTa > 1:2136544/1‑69 (MQ=255) gttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTGTATGCGTTCATCAGTTa > 1:3341544/1‑69 (MQ=255) gttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTGTATGCGTTCATCAGTTa > 1:2073140/1‑69 (MQ=255) gttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTGTATGCGTTCATCAGTTa > 1:686490/1‑69 (MQ=255) gttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTGTATGCGTTCATCAGTTa > 1:2835637/1‑69 (MQ=255) gttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTGTATGCGTTCATCAGTTa > 1:363833/1‑69 (MQ=255) gttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTGTATGCGTTCATCAGTTa > 1:1102450/1‑69 (MQ=255) gttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTGTATGCGTTCATCAGTTa > 1:1604199/1‑69 (MQ=255) gttgATGCTGTTTGGTTTCTCCGCCAGCGGCAACATTGAAGTGGGCGTGTTGTATGCGTTCATCAGTTa > 1:118212/1‑69 (MQ=255) ttgATGCTGTTTGGTTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGTTg < 1:2648460/51‑1 (MQ=255) gCTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACtt < 1:845832/68‑1 (MQ=255) gCTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACtt < 1:3290266/68‑1 (MQ=255) gCTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACtt < 1:2412363/68‑1 (MQ=255) gCTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACtt < 1:1282964/68‑1 (MQ=255) gCTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACtt < 1:2154627/68‑1 (MQ=255) gCTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACtt < 1:2144503/68‑1 (MQ=255) gCTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACtt < 1:3417329/68‑1 (MQ=255) gCTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACtt < 1:1896420/68‑1 (MQ=255) gCTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACtt < 1:1643052/68‑1 (MQ=255) gCTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACtt < 1:1572628/68‑1 (MQ=255) gCTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTa > 1:972547/1‑69 (MQ=255) gCTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTa > 1:470430/1‑69 (MQ=255) gCTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTa > 1:1189131/1‑69 (MQ=255) tctcCGCCAGCGGCACCATTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACg < 1:1023223/69‑1 (MQ=255) tctcCGCCAGCGGCACCATTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACg < 1:1550507/69‑1 (MQ=255) tctcCGCCAGCGGCACCATTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACg < 1:364446/69‑1 (MQ=255) ctcCGCCAGCGGCACCATTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACg < 1:3380791/68‑1 (MQ=255) cATTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACt < 1:2992111/69‑1 (MQ=255) cATTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACt < 1:2119226/69‑1 (MQ=255) cATTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACt < 1:1593791/69‑1 (MQ=255) cATTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACt < 1:3041379/69‑1 (MQ=255) aTTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTg > 1:2001023/1‑69 (MQ=255) aTTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTg > 1:150898/1‑69 (MQ=255) aTTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTg > 1:540114/1‑69 (MQ=255) aTTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTg > 1:1833130/1‑69 (MQ=255) aTTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTg > 1:889489/1‑69 (MQ=255) aTTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTg > 1:3382510/1‑69 (MQ=255) aTTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTg > 1:3354721/1‑69 (MQ=255) aTTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTg > 1:2398061/1‑69 (MQ=255) aTTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTg > 1:2600773/1‑69 (MQ=255) aTTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTg > 1:954389/1‑69 (MQ=255) aaGTGGGCGTGTTGTATGCGTTCATCAGTTATTTGGGGCGACTTAACGAACCCTTAATc > 1:45789/1‑59 (MQ=255) aaGTGGGCGTGTTGTATGCGTTCATCAGTTATTTGGGGCGACTTAACGAACCCTTAATc > 1:1026001/1‑59 (MQ=255) aaGTGGGCGTGTTGTATGCGTTCATCAGTTATTTGGGGCGACTTAACGAACCCTTAATc > 1:1295433/1‑59 (MQ=255) aaGTGGGCGTGTTGTATGCGTTCATCAGTTATTTGGGGCGACTTAACGAACCCTTAATc > 1:1365593/1‑59 (MQ=255) aaGTGGGCGTGTTGTATGCGTTCATCAGTTATTTGGGGCGACTTAACGAACCCTTAATc > 1:1853799/1‑59 (MQ=255) aaGTGGGCGTGTTGTATGCGTTCATCAGTTATTTGGGGCGACTTAACGAACCCTTAATc > 1:3057677/1‑59 (MQ=255) gCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGc < 1:1444875/69‑1 (MQ=255) gCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGc < 1:484660/69‑1 (MQ=255) gCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGc < 1:903848/69‑1 (MQ=255) gCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGc < 1:1143745/69‑1 (MQ=255) gCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGc < 1:3027765/69‑1 (MQ=255) cTGTATGCGTTTATCAGCTTTGTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGc < 1:2704840/68‑1 (MQ=255) | TCATTCTTTGTGGCTTGTTGATGCTGTTTGGCTTCTCCGCCAGCGGCACCATTGAAGTGGGCGTGCTGTATGCGTTTATCAGCTATCTTGGGCGACTTAACGAACCATTAATCGAACTGACCACGCAACAGGC > minE/299889‑300021 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |