Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 300,682 C→T 31.1% T526T (ACC→ACT mdlB → fused predicted multidrug transporter subunits and ATP binding components of ABC superfamily

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE300,6820CT31.1% 87.4 / 43.8 93T526T (ACC→ACTmdlBfused predicted multidrug transporter subunits and ATP binding components of ABC superfamily
Reads supporting (aligned to +/- strand):  ref base C (21/43);  new base T (15/14);  total (36/57)
Fisher's exact test for biased strand distribution p-value = 1.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.08e-01

GCATTGACTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCATTGTTGATGCCGACACCATTCTGGTGCTTCAT  >  minE/300621‑300748
                                                             |                                                                  
gCATTGACTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTgg                                                             <  1:3203838/69‑1 (MQ=255)
gCATTGACTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTgg                                                             <  1:1027126/69‑1 (MQ=255)
gCATTGACTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTgg                                                             <  1:219287/69‑1 (MQ=255)
gCATTGACTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTgg                                                             <  1:1610946/69‑1 (MQ=255)
 cATTGACTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGt                                                            <  1:121381/69‑1 (MQ=255)
  aTTGACTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTa                                                           >  1:1411970/1‑69 (MQ=255)
     gACTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGt                                                         <  1:278696/68‑1 (MQ=255)
     gACTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGt                                                         <  1:1936484/68‑1 (MQ=255)
     gACTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGt                                                         <  1:1700860/68‑1 (MQ=255)
     gACTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTg                                                        <  1:136015/69‑1 (MQ=255)
     gACTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTg                                                        <  1:1409302/69‑1 (MQ=255)
     gACTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTg                                                        <  1:842515/69‑1 (MQ=255)
            gTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCt                                                  <  1:2215501/68‑1 (MQ=255)
            gTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCt                                                  <  1:3010939/68‑1 (MQ=255)
              aCTGAACAGGCGATTCAACATGCTCTTGCGGCGGTGCGTGAACATACCACGCTGGTAg                                                          <  1:1227525/58‑1 (MQ=255)
              aCTGAACAGGCGATTCAACATGCTCTGGCGGCGTTGCGTGAACATACCACGCTGGt                                                            >  1:2990377/1‑56 (MQ=255)
              aCTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGt                                                            >  1:1399606/1‑56 (MQ=255)
              aCTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGt                                                            >  1:3124239/1‑56 (MQ=255)
              aCTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGt                                                            >  1:1167172/1‑56 (MQ=255)
              aCTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGt                                                            >  1:3268502/1‑56 (MQ=255)
              aCTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGt                                                            >  1:3090057/1‑56 (MQ=255)
              aCTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGt                                                            >  1:3388911/1‑56 (MQ=255)
              aCTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGt                                                            >  1:781266/1‑56 (MQ=255)
              aCTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGt                                                            >  1:2475513/1‑56 (MQ=255)
              aCTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGt                                                            >  1:1541659/1‑56 (MQ=255)
              aCTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGt                                                            >  1:2656253/1‑56 (MQ=255)
              aCTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGt                                                            >  1:623032/1‑56 (MQ=255)
              aCTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGt                                                            >  1:74758/1‑56 (MQ=255)
              aCTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGt                                                            >  1:778191/1‑56 (MQ=255)
              aCTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAg                                                          <  1:2618242/58‑1 (MQ=255)
              aCTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAg                                                          <  1:2203498/58‑1 (MQ=255)
              aCTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAg                                                          <  1:1700780/58‑1 (MQ=255)
              aCTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAg                                                          <  1:2037962/58‑1 (MQ=255)
              aCTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAg                                                          <  1:3256273/58‑1 (MQ=255)
              aCTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAg                                                          <  1:1840581/58‑1 (MQ=255)
              aCTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAg                                                          <  1:3335836/58‑1 (MQ=255)
              aCTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAg                                                          <  1:547977/58‑1 (MQ=255)
              aCTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAg                                                          <  1:1502438/58‑1 (MQ=255)
              aCTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAg                                                          <  1:983512/58‑1 (MQ=255)
              aCTGAACAGGCGATTCAACATGCTCTGGCGGCCGTGCGTGAACATACCACGCTgg                                                             >  1:3405693/1‑55 (MQ=255)
                    cAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCt                                           <  1:2872670/67‑1 (MQ=255)
                    cAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTa                                         >  1:3314759/1‑69 (MQ=255)
                    cAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTa                                         >  1:156009/1‑69 (MQ=255)
                    cAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTa                                         >  1:1092259/1‑69 (MQ=255)
                            tCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCAt                                 <  1:2839023/69‑1 (MQ=255)
                            tCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCAt                                 <  1:1293625/69‑1 (MQ=255)
                            tCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCAt                                 >  1:1342373/1‑69 (MQ=255)
                             cAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCAt                                 <  1:1503846/68‑1 (MQ=255)
                                cATGCTCTGGCGGCGGTGCGTGACCATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCAttgtt                             <  1:2105588/69‑1 (MQ=255)
                                cATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCAttgtt                             <  1:1705179/69‑1 (MQ=255)
                                cATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCAttgtt                             <  1:1075887/69‑1 (MQ=255)
                                cATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCAttgtt                             <  1:1433220/69‑1 (MQ=255)
                                cATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCAttgtt                             <  1:616377/69‑1 (MQ=255)
                                cATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCAttgtt                             <  1:3402277/69‑1 (MQ=255)
                                cATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCAttgtt                             <  1:1672518/69‑1 (MQ=255)
                                cATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCAttgtt                             <  1:2032721/69‑1 (MQ=255)
                                cATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCAttgtt                             <  1:3207090/69‑1 (MQ=255)
                                cATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCAttgtt                             <  1:2937090/69‑1 (MQ=255)
                                cATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCAttgtt                             <  1:3071693/69‑1 (MQ=255)
                                cATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCAttgtt                             <  1:2949197/69‑1 (MQ=255)
                                cATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCAttgtt                             <  1:2604063/69‑1 (MQ=255)
                                cATGCTCTGGCGGCGGTGCGTGAACATACCAAGCTGGTAGTGATTGCTCACCGCTTATCGACCAttgtt                             <  1:3371751/69‑1 (MQ=255)
                                 aTGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAg                                                          <  1:1959565/39‑1 (MQ=255)
                                  tGCTCTGGCGGCGGTGCGTGAACATACTACGCTTGTGGTGATTGCTCACCGCTTATCAACTAttgttg                            >  1:2225548/1‑68 (MQ=255)
                                  tGCTCTGGCGGCGGTGCGTGAACATACTACGCTTGTGGTGATTGCTCACCGCTTATCAACTAttgttg                            >  1:2960243/1‑68 (MQ=255)
                                  tGCTCTGGCGGCGGTGCGTGAACATACTACGCTTGTGGTGATTGCTCACCGCTTATCAACTATTGTTGa                           >  1:1546866/1‑69 (MQ=255)
                                  tGCTCTGGCGGCGGTGCGTGAACATACTACGCTTGTGGTGATTGCTCACCGCTTATCAACTATTGTTGa                           >  1:2775384/1‑69 (MQ=255)
                                  tGCTCTGGCGGCGGTGCGTGAACATACTACGCTTGTGGTGATTGCTCACCGCTTATCAACTATTGTTGa                           >  1:943073/1‑69 (MQ=255)
                                  tGCTCTGGCGGCGGTGCGTGAACATACTACGCTTGTGGTGATTGCTCACCGCTTATCAACTATTGTTGa                           >  1:939099/1‑69 (MQ=255)
                                  tGCTCTGGCGGCGGTGCGTGAACATACTACGCTTGTGGTGATTGCTCACCGCTTATCAACTATTGTTGa                           >  1:3353573/1‑69 (MQ=255)
                                  tGCTCTGGCGGCGGTGCGTGAACATACTACGCTTGTGGTGATTGCTCACCGCTTATCAACTATTGTTGa                           >  1:1927423/1‑69 (MQ=255)
                                  tGCTCTGGCGGCGGTGCGTGAACATACTACGCTTGTGGTGATTGCTCACCGCTTATCAACTATTGTTGa                           >  1:3093038/1‑69 (MQ=255)
                                  tGCTCTGGCGGCGGTGCGTGAACATACTACGCTTGTGGTGATTGCTCACCGCTTATCAACTATTGTTGa                           >  1:309476/1‑69 (MQ=255)
                                  tGCTCTGGCGGCGGTGCGTGAACATACTACGCTTGTGGTGATTGCTCACCGCTTATCAACTATTGTTGa                           >  1:3183564/1‑69 (MQ=255)
                                  tGCTCTGGCGGCGGTGCGTGAACATACTACGCTTGTGGTGATTGCTCACCGCTTATCAACTATTGTTGa                           >  1:3021879/1‑69 (MQ=255)
                                  tGCTCTGGCGGCGGTGCGTGAACATACTACGCTTGTGGTGATTGCTCACCGCTTATCAACTATTGTTGa                           >  1:32684/1‑69 (MQ=255)
                                  tGCTCTGGCGGCGGTGCGTGAACATACTACGCTTGTGGTGATTGCTCACCGCTTATCAACTATTGTTGa                           >  1:2805050/1‑69 (MQ=255)
                                  tGCTCTGGCGGCGGTGCGTGAACATACTACGCTTGTGGTGATTGCTCACCGCTTATCAACTATTGTTGa                           >  1:3301675/1‑69 (MQ=255)
                                      ctGGCGGCGGTGCGTGAACATACTACGCTTGTGGTGATTGCTCACCGCTTATCAACTATTGTTGATGcc                       <  1:779700/69‑1 (MQ=255)
                                      ctGGCGGCGGTGCGTGAACATACTACGCTTGTGGTGATTGCTCACCGCTTATCAACTATTGTTGATGcc                       <  1:337972/69‑1 (MQ=255)
                                      ctGGCGGCGGTGCGTGAACATACTACGCTTGTGGTGATTGCTCACCGCTTATCAACTATTGTTGATGcc                       <  1:2136679/69‑1 (MQ=255)
                                      ctGGCGGCGGTGCGTGAACATACTACGCTTGTGGTGATTGCTCACCGCTTATCAACTATTGTTGATGcc                       <  1:932282/69‑1 (MQ=255)
                                      ctGGCGGCGGTGCGTGAACATACTACGCTTGTGGTGATTGCTCACCGCTTATCAACTATTGTTGATGcc                       <  1:894871/69‑1 (MQ=255)
                                      ctGGCGGCGGTGCGTGAACATACTACGCTTGTGGTGATTGCTCACCGCTTATCAACTATTGTTGATGcc                       <  1:2631692/69‑1 (MQ=255)
                                      ctGGCGGCGGTGCGTGAACATACTACGCTTGTGGTGATTGCTCACCGCTTATCAACTATTGTTGATGcc                       <  1:785189/69‑1 (MQ=255)
                                      ctGGCGGCGGTGCGTGAACATACTACGCTTGTGGTGATTGCTCACCGCTTATCAACTATTGTTGATGcc                       <  1:1308391/69‑1 (MQ=255)
                                      ctGGCGGCGGTGCGTGAACATACTACGCTTGTGGTGATTGCTCACCGCTTATCAACTATTGTTGATGcc                       <  1:351162/69‑1 (MQ=255)
                                      ctGGCGGCGGTGCGTGAACATACTACGCTTGTGGTGATTGCTCACCGCTTATCAACTATTGTTGATGcc                       <  1:770031/69‑1 (MQ=255)
                                      ctGGCGGCGGTGCGTGAACATACTACGCTTGTGGTGATTGCTCACCGCTTATCAACTATTGTTGATGcc                       <  1:1421842/69‑1 (MQ=255)
                                      ctGGCGGCGGTGCGTGAACATACTACGCTTGTGGTGATTGCTCACCGCTTATCAACTATTGTTGATGcc                       <  1:638221/69‑1 (MQ=255)
                                      ctGGCGGCGGTGCGTGAACATACTACGCTTGTGGTGATTGCTCACCGCTTATCAACTATTGTTGATGcc                       <  1:3010598/69‑1 (MQ=255)
                                      ctGGCGGCGGTGCGTGAACATACTACGCTTGTGGTGATTGCTCACCGCTTATCAACTATTGTTGATGcc                       <  1:2220236/69‑1 (MQ=255)
                                                           aCCACGCTGGTAGTGATTGCTCACCGCTTATCGACCATTGTTGATGCCGACACCATTCTGGTGCTTCAt  >  1:2910957/1‑69 (MQ=255)
                                                             |                                                                  
GCATTGACTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCATTGTTGATGCCGACACCATTCTGGTGCTTCAT  >  minE/300621‑300748

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: