Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 300,691 A→G 40.2% V529V (GTA→GTG mdlB → fused predicted multidrug transporter subunits and ATP binding components of ABC superfamily

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE300,6910AG40.2% 30.4 / 50.4 72V529V (GTA→GTGmdlBfused predicted multidrug transporter subunits and ATP binding components of ABC superfamily
Reads supporting (aligned to +/- strand):  ref base A (5/38);  new base G (15/14);  total (20/52)
Fisher's exact test for biased strand distribution p-value = 3.46e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.54e-01

ATTGACTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCATTGTTGATGCCGACACCATTCTGGTGCTTCATCGTGGGCAAGC  >  minE/300623‑300759
                                                                    |                                                                    
aTTGACTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTa                                                                      >  1:1411970/1‑69 (MQ=255)
   gACTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGt                                                                    <  1:1936484/68‑1 (MQ=255)
   gACTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGt                                                                    <  1:1700860/68‑1 (MQ=255)
   gACTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGt                                                                    <  1:278696/68‑1 (MQ=255)
   gACTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTg                                                                   <  1:842515/69‑1 (MQ=255)
   gACTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTg                                                                   <  1:136015/69‑1 (MQ=255)
   gACTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTg                                                                   <  1:1409302/69‑1 (MQ=255)
          gTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCt                                                             <  1:2215501/68‑1 (MQ=255)
          gTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCt                                                             <  1:3010939/68‑1 (MQ=255)
            aCTGAACAGGCGATTCAACATGCTCTTGCGGCGGTGCGTGAACATACCACGCTGGTAg                                                                     <  1:1227525/58‑1 (MQ=255)
            aCTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAg                                                                     <  1:1502438/58‑1 (MQ=255)
            aCTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAg                                                                     <  1:2037962/58‑1 (MQ=255)
            aCTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAg                                                                     <  1:2203498/58‑1 (MQ=255)
            aCTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAg                                                                     <  1:1840581/58‑1 (MQ=255)
            aCTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAg                                                                     <  1:1700780/58‑1 (MQ=255)
            aCTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAg                                                                     <  1:2618242/58‑1 (MQ=255)
            aCTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAg                                                                     <  1:3256273/58‑1 (MQ=255)
            aCTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAg                                                                     <  1:3335836/58‑1 (MQ=255)
            aCTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAg                                                                     <  1:547977/58‑1 (MQ=255)
            aCTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAg                                                                     <  1:983512/58‑1 (MQ=255)
                  cAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCt                                                      <  1:2872670/67‑1 (MQ=255)
                  cAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTa                                                    >  1:1092259/1‑69 (MQ=255)
                  cAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTa                                                    >  1:3314759/1‑69 (MQ=255)
                  cAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTa                                                    >  1:156009/1‑69 (MQ=255)
                          tCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCAt                                            <  1:1293625/69‑1 (MQ=255)
                          tCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCAt                                            <  1:2839023/69‑1 (MQ=255)
                          tCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCAt                                            >  1:1342373/1‑69 (MQ=255)
                           cAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCAt                                            <  1:1503846/68‑1 (MQ=255)
                              cATGCTCTGGCGGCGGTGCGTGACCATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCAttgtt                                        <  1:2105588/69‑1 (MQ=255)
                              cATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCAttgtt                                        <  1:1672518/69‑1 (MQ=255)
                              cATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCAttgtt                                        <  1:2949197/69‑1 (MQ=255)
                              cATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCAttgtt                                        <  1:2937090/69‑1 (MQ=255)
                              cATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCAttgtt                                        <  1:3071693/69‑1 (MQ=255)
                              cATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCAttgtt                                        <  1:3207090/69‑1 (MQ=255)
                              cATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCAttgtt                                        <  1:1433220/69‑1 (MQ=255)
                              cATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCAttgtt                                        <  1:2604063/69‑1 (MQ=255)
                              cATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCAttgtt                                        <  1:3402277/69‑1 (MQ=255)
                              cATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCAttgtt                                        <  1:616377/69‑1 (MQ=255)
                              cATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCAttgtt                                        <  1:1705179/69‑1 (MQ=255)
                              cATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCAttgtt                                        <  1:1075887/69‑1 (MQ=255)
                              cATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCAttgtt                                        <  1:2032721/69‑1 (MQ=255)
                              cATGCTCTGGCGGCGGTGCGTGAACATACCAAGCTGGTAGTGATTGCTCACCGCTTATCGACCAttgtt                                        <  1:3371751/69‑1 (MQ=255)
                               aTGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAg                                                                     <  1:1959565/39‑1 (MQ=255)
                                tGCTCTGGCGGCGGTGCGTGAACATACTACGCTTGTGGTGATTGCTCACCGCTTATCAACTAttgttg                                       >  1:2960243/1‑68 (MQ=255)
                                tGCTCTGGCGGCGGTGCGTGAACATACTACGCTTGTGGTGATTGCTCACCGCTTATCAACTAttgttg                                       >  1:2225548/1‑68 (MQ=255)
                                tGCTCTGGCGGCGGTGCGTGAACATACTACGCTTGTGGTGATTGCTCACCGCTTATCAACTATTGTTGa                                      >  1:32684/1‑69 (MQ=255)
                                tGCTCTGGCGGCGGTGCGTGAACATACTACGCTTGTGGTGATTGCTCACCGCTTATCAACTATTGTTGa                                      >  1:1927423/1‑69 (MQ=255)
                                tGCTCTGGCGGCGGTGCGTGAACATACTACGCTTGTGGTGATTGCTCACCGCTTATCAACTATTGTTGa                                      >  1:3353573/1‑69 (MQ=255)
                                tGCTCTGGCGGCGGTGCGTGAACATACTACGCTTGTGGTGATTGCTCACCGCTTATCAACTATTGTTGa                                      >  1:939099/1‑69 (MQ=255)
                                tGCTCTGGCGGCGGTGCGTGAACATACTACGCTTGTGGTGATTGCTCACCGCTTATCAACTATTGTTGa                                      >  1:3301675/1‑69 (MQ=255)
                                tGCTCTGGCGGCGGTGCGTGAACATACTACGCTTGTGGTGATTGCTCACCGCTTATCAACTATTGTTGa                                      >  1:2775384/1‑69 (MQ=255)
                                tGCTCTGGCGGCGGTGCGTGAACATACTACGCTTGTGGTGATTGCTCACCGCTTATCAACTATTGTTGa                                      >  1:2805050/1‑69 (MQ=255)
                                tGCTCTGGCGGCGGTGCGTGAACATACTACGCTTGTGGTGATTGCTCACCGCTTATCAACTATTGTTGa                                      >  1:3183564/1‑69 (MQ=255)
                                tGCTCTGGCGGCGGTGCGTGAACATACTACGCTTGTGGTGATTGCTCACCGCTTATCAACTATTGTTGa                                      >  1:309476/1‑69 (MQ=255)
                                tGCTCTGGCGGCGGTGCGTGAACATACTACGCTTGTGGTGATTGCTCACCGCTTATCAACTATTGTTGa                                      >  1:3093038/1‑69 (MQ=255)
                                tGCTCTGGCGGCGGTGCGTGAACATACTACGCTTGTGGTGATTGCTCACCGCTTATCAACTATTGTTGa                                      >  1:1546866/1‑69 (MQ=255)
                                tGCTCTGGCGGCGGTGCGTGAACATACTACGCTTGTGGTGATTGCTCACCGCTTATCAACTATTGTTGa                                      >  1:943073/1‑69 (MQ=255)
                                tGCTCTGGCGGCGGTGCGTGAACATACTACGCTTGTGGTGATTGCTCACCGCTTATCAACTATTGTTGa                                      >  1:3021879/1‑69 (MQ=255)
                                    ctGGCGGCGGTGCGTGAACATACTACGCTTGTGGTGATTGCTCACCGCTTATCAACTATTGTTGATGcc                                  <  1:3010598/69‑1 (MQ=255)
                                    ctGGCGGCGGTGCGTGAACATACTACGCTTGTGGTGATTGCTCACCGCTTATCAACTATTGTTGATGcc                                  <  1:932282/69‑1 (MQ=255)
                                    ctGGCGGCGGTGCGTGAACATACTACGCTTGTGGTGATTGCTCACCGCTTATCAACTATTGTTGATGcc                                  <  1:894871/69‑1 (MQ=255)
                                    ctGGCGGCGGTGCGTGAACATACTACGCTTGTGGTGATTGCTCACCGCTTATCAACTATTGTTGATGcc                                  <  1:2136679/69‑1 (MQ=255)
                                    ctGGCGGCGGTGCGTGAACATACTACGCTTGTGGTGATTGCTCACCGCTTATCAACTATTGTTGATGcc                                  <  1:785189/69‑1 (MQ=255)
                                    ctGGCGGCGGTGCGTGAACATACTACGCTTGTGGTGATTGCTCACCGCTTATCAACTATTGTTGATGcc                                  <  1:779700/69‑1 (MQ=255)
                                    ctGGCGGCGGTGCGTGAACATACTACGCTTGTGGTGATTGCTCACCGCTTATCAACTATTGTTGATGcc                                  <  1:770031/69‑1 (MQ=255)
                                    ctGGCGGCGGTGCGTGAACATACTACGCTTGTGGTGATTGCTCACCGCTTATCAACTATTGTTGATGcc                                  <  1:638221/69‑1 (MQ=255)
                                    ctGGCGGCGGTGCGTGAACATACTACGCTTGTGGTGATTGCTCACCGCTTATCAACTATTGTTGATGcc                                  <  1:2220236/69‑1 (MQ=255)
                                    ctGGCGGCGGTGCGTGAACATACTACGCTTGTGGTGATTGCTCACCGCTTATCAACTATTGTTGATGcc                                  <  1:1308391/69‑1 (MQ=255)
                                    ctGGCGGCGGTGCGTGAACATACTACGCTTGTGGTGATTGCTCACCGCTTATCAACTATTGTTGATGcc                                  <  1:351162/69‑1 (MQ=255)
                                    ctGGCGGCGGTGCGTGAACATACTACGCTTGTGGTGATTGCTCACCGCTTATCAACTATTGTTGATGcc                                  <  1:337972/69‑1 (MQ=255)
                                    ctGGCGGCGGTGCGTGAACATACTACGCTTGTGGTGATTGCTCACCGCTTATCAACTATTGTTGATGcc                                  <  1:2631692/69‑1 (MQ=255)
                                    ctGGCGGCGGTGCGTGAACATACTACGCTTGTGGTGATTGCTCACCGCTTATCAACTATTGTTGATGcc                                  <  1:1421842/69‑1 (MQ=255)
                                                         aCCACGCTGGTAGTGATTGCTCACCGCTTATCGACCATTGTTGATGCCGACACCATTCTGGTGCTTCAt             >  1:2910957/1‑69 (MQ=255)
                                                                    aGTGATTGCTCACCGCTTATCGACCATTGTTGATGCCGACACCATTCTGGTGCTTCATCGTGGGCAAGc  >  1:587447/1‑69 (MQ=255)
                                                                    aGTGATTGCTCACCGCTTATCGACCATTGTTGATGCCGACACCATTCTGGTGCTTCATCGTGGGCAAGc  >  1:2260364/1‑69 (MQ=255)
                                                                    aGTGATTGCTCACCGCTTATCGACCATTGTTGATGCCGACACCATTCTGGTGCTTCATCGTGGGCAAGc  >  1:3035193/1‑69 (MQ=255)
                                                                    |                                                                    
ATTGACTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCATTGTTGATGCCGACACCATTCTGGTGCTTCATCGTGGGCAAGC  >  minE/300623‑300759

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: