| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 34,063 | A→G | 41.5% | F485F (TTT→TTC) | caiC ← | predicted crotonobetaine CoA ligase:carnitine CoA ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 34,063 | 0 | A | G | 41.5% | 19.3 / 46.5 | 65 | F485F (TTT→TTC) | caiC | predicted crotonobetaine CoA ligase:carnitine CoA ligase |
| Reads supporting (aligned to +/- strand): ref base A (19/19); new base G (15/12); total (34/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.02e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01 | |||||||||||
CTTTTCTGATCTCCAGATAAGAGGGCACTTTAAATTTCGCCATATTTTGTTCGCAGAAGCGGAAAAATTCCTCTTCGCTCAATGTTTCACCTTCATTCAGCACCACAAATGCT > minE/33999‑34111 | cTTTTCTGATCTCCAGATAAGAGGGCACTTTAAATTTCGCCATATTTTGTTCGCAGAAGCGGAAAAAtt > 1:1035703/1‑69 (MQ=255)cTTTTCTGATCTCCAGATAAGAGGGCACTTTAAATTTCGCCATATTTTGTTCGCAGAAGCGGAAAAAtt > 1:2199895/1‑69 (MQ=255) cTGATCTCCAGATAAGAGGGCACTTTAAATTTCGCCATATTTTGTTCGCAGAAGCGGAAAAATTc < 1:2563313/65‑1 (MQ=255) cTGATCTCCAGATAAGAGGGCACTTTAAATTTCGCCATATTTTGTTCGCAGAAGCGGAAAAATTc < 1:2549786/65‑1 (MQ=255) tGATCTCCAGATAAGAGGGCACTTTAAATTTCGCCATATTTTGTTCGCAGAAGCGGAAAAATTc < 1:2579541/64‑1 (MQ=255) gATAAGAGGGCACTTTAAATTTCGCCATATTCTGTTCACAGAAGCAGAAGAATTCCTCTTCGCTc < 1:213673/65‑1 (MQ=255) gATAAGAGGGCACTTTAAATTTCGCCATATTCTGTTCACAGAAGCAGAAGAATTCCTCTTCGCTc < 1:2656317/65‑1 (MQ=255) gATAAGAGGGCACTTTAAATTTCGCCATATTCTGTTCACAGAAGCAGAAGAATTCCTCTTCGCTc < 1:2586/65‑1 (MQ=255) gATAAGAGGGCACTTTAAATTTCGCCATATTCTGTTCACAGAAGCAGAAGAATTCCTCTTCGCTc < 1:2537328/65‑1 (MQ=255) gATAAGAGGGCACTTTAAATTTCGCCATATTCTGTTCACAGAAGCAGAAGAATTCCTCTTCGCTc < 1:2016752/65‑1 (MQ=255) gATAAGAGGGCACTTTAAATTTCGCCATATTCTGTTCACAGAAGCAGAAGAATTCCTCTTCGCTc < 1:302927/65‑1 (MQ=255) gATAAGAGGGCACTTTAAATTTCGCCATATTCTGTTCACAGAAGCAGAAGAATTCCTCTTCGCTc < 1:1664055/65‑1 (MQ=255) gATAAGAGGGCACTTTAAATTTCGCCATATTCTGTTCACAGAAGCAGAAGAATTCCTCTTCGCTc < 1:130284/65‑1 (MQ=255) gATAAGAGGGCACTTTAAATTTCGCCATATTCTGTTCACAGAAGCAGAAGAATTCCTCTTCGCTc < 1:3086953/65‑1 (MQ=255) gATAAGAGGGCACTTTAAATTTCGCCATATTCTGTTCACAGAAGAAGAAGAATTCCTCTTCGCTc < 1:1963413/65‑1 (MQ=255) aTAAGAGGGCACTTTAAATTTCGCCATATTCTGTTCACAGAAGCAGAAGAATTCCTCTTCGCTc < 1:251102/64‑1 (MQ=255) aaGAGGGCACTTTAAATTTCGCCATATTTTGTTCGCAGAAGCGGAAAAATTCCTCTTCGCTCaa < 1:2929916/64‑1 (MQ=255) aaGAGGGCACTTTAAATTTCGCCATATTTTGTTCGCAGAAGCGGAAAAATTCCTCTTCGCTCaa < 1:2281558/64‑1 (MQ=255) aaGAGGGCACTTTAAATTTCGCCATATTTTGTTCGCAGAAGCGGAAAAATTCCTCTTCGCTCaa < 1:2086864/64‑1 (MQ=255) aaGAGGGCACTTTAAATTTCGCCATATTTTGTTCGCAGAAGCGGAAAAATTCCTCTTCGCTCaa < 1:2653237/64‑1 (MQ=255) aaGAGGGCACTTTAAATTTCGCCATATTTTGTTCGCAGAAGCGGAAAAATTCCTCTTCGCTCaa < 1:1273347/64‑1 (MQ=255) aaGAGGGCACTTTAAATTTCGCCATATTTTGTTCGCAGAAGCGGAAAAATTCCTCTTCGCTCaa < 1:1793624/64‑1 (MQ=255) aaGAGGGCACTTTAAATTTCGCCATATTTTGTTCGCAGAAGCGGAAAAATTCCTCTTCGCTCaa < 1:2717591/64‑1 (MQ=255) agGGCACTTTAAATTTCGCCATATTTTGTTCGCAGAAGCGGAAAAATTCCTCTTCGCTCAATGTTTCAc > 1:826738/1‑69 (MQ=255) agGGCACTTTAAATTTCGCCATATTTTGTTCGCAGAAGCGGAAAAATTCCTCTTCGCTCAATGTTTCAc > 1:851926/1‑69 (MQ=255) agGGCACTTTAAATTTCGCCATATTTTGTTCGCAGAAGCGGAAAAATTCCTCTTCGCTCAATGTTTCAc > 1:2828453/1‑69 (MQ=255) agGGCACTTTAAATTTCGCCATATTTTGTTCGCAGAAGCGGAAAAATTCCTCTTCGCTCAATGTTTCAc > 1:847984/1‑69 (MQ=255) gCACTTTAAATTTCGCCATATTTTGTTCGCAGAAGCGGAAAAATTCCTCTTCGCTCAATGTTTCACCtt < 1:2631179/69‑1 (MQ=255) gCACTTTAAATTTCGCCATATTTTGTTCGCAGAAGCGGAAAAATTCCTCTTCGCTCAATGTTTCACCtt < 1:1345692/69‑1 (MQ=255) gCACTTTAAATTTCGCCATATTTTGTTCGCAGAAGCGGAAAAATTCCTCTTCGCTCAATGTTTCACCtt < 1:218100/69‑1 (MQ=255) gCACTTTAAATTTCGCCATATTTTGTTCGCAGAAGCGGAAAAATTCCTCTTCGCTCAATGTTTCACCtt < 1:96204/69‑1 (MQ=255) cACTTTAAATTTCGCCATATTTTGTTCGCAGAAGCGGAAAAATTCCTCTTCGCTCAATGTTTCACCtt < 1:262235/68‑1 (MQ=255) tttAAATTTCGCCATATTCTGTTCACAGAAGCAGAAGAATTCCTCTTCGCTc < 1:2591434/52‑1 (MQ=255) ccATATTTTGTTCGCAGAAGCGGAAAAATTCCTCTTCGCTCAATGTTTCACCTTCATTCAGCACcaca < 1:2684036/68‑1 (MQ=255) ccATATTTTGTTCGCAGAAGCGGAAAAATTCCTCTTCGCTCAATGTTTCACCTTCATTCAGCACcaca < 1:1596978/68‑1 (MQ=255) ccATATTTTGTTCGCAGAAGCGGAAAAATTCCTCTTCGCTCAATGTTTCACCTTCATTCAGCACcaca < 1:2166235/68‑1 (MQ=255) ccATATTTTGTTCGCAGAAGCGGAAAAATTCCTCTTCGCTCAATGTTTCACCTTCATTCAGCACcaca < 1:372740/68‑1 (MQ=255) atTCTGTTCACATAAGCAGAAGAATTCCTCTTCGCTCAATGTTTCACCTTCATTCAGCACCACAAATGc > 1:1664905/1‑69 (MQ=255) atTCTGTTCACAGAAGCAGAAGAATTCCTCTTCGGTCAATGTTTCACCTTCATTCAGCACCACAAATGc > 1:2860974/1‑69 (MQ=255) atTCTGTTCACAGAAGCAGAAGAATTCCTCTTCGCTCAATGt > 1:174789/1‑42 (MQ=25) atTCTGTTCACAGAAGCAGAAGAATTCCTCTTCGCTCAATGTTTCACCTTCATTCAGCACCACAAATGc > 1:2766186/1‑69 (MQ=255) atTCTGTTCACAGAAGCAGAAGAATTCCTCTTCGCTCAATGTTTCACCTTCATTCAGCACCACAAATGc > 1:1036620/1‑69 (MQ=255) atTCTGTTCACAGAAGCAGAAGAATTCCTCTTCGCTCAATGTTTCACCTTCATTCAGCACCACAAATGc > 1:2960105/1‑69 (MQ=255) atTCTGTTCACAGAAGCAGAAGAATTCCTCTTCGCTCAATGTTTCACCTTCATTCAGCACCACAAATGc > 1:2547904/1‑69 (MQ=255) atTCTGTTCACAGAAGCAGAAGAATTCCTCTTCGCTCAATGTTTCACCTTCATTCAGCACCACAAATGc > 1:2477517/1‑69 (MQ=255) atTCTGTTCACAGAAGCAGAAGAATTCCTCTTCGCTCAATGTTTCACCTTCATTCAGCACCACAAATGc > 1:2215805/1‑69 (MQ=255) atTCTGTTCACAGAAGCAGAAGAATTCCTCTTCGCTCAATGTTTCACCTTCATTCAGCACCACAAATGc > 1:2144981/1‑69 (MQ=255) atTCTGTTCACAGAAGCAGAAGAATTCCTCTTCGCTCAATGTTTCACCTTCATTCAGCACCACAAATGc > 1:1928805/1‑69 (MQ=255) atTCTGTTCACAGAAGCAGAAGAATTCCTCTTCGCTCAATGTTTCACCTTCATTCAGCACCACAAATGc > 1:1278273/1‑69 (MQ=255) atTCTGTTCACAGAAGCAGAAGAATTCCTCTTCGCTCAATGTTTCACCTTCATTCAGCACCACAAATGc > 1:1588034/1‑69 (MQ=255) atTCTGTTCACAGAAGCAGAAGAATTCCTCTTCGCTCAATGTTTCACCTTCATTCAGCACCACAAATGc > 1:1541815/1‑69 (MQ=255) atTCTGTTCACAGAAGCAGAAGAATTCCTCTTCGCTCAATGTTTCACCTTCATTCAGCACCACAAATGc > 1:145093/1‑69 (MQ=255) ttttttTCGCAGAAGCGGAAAAATTCCTCTTCGCTCAATGTTTCACCTTCATTCAGCACCACAAATGCt > 1:1445782/1‑69 (MQ=255) ttttGTTCGCAGAAGCGGAAAAATTCCTCTTCGCTCAATGTTTCACCtt > 1:990473/1‑49 (MQ=255) ttttGTTCGCAGAAGCGGAAAAATTCCTCTTCGCTCAATGTTTCACCtt > 1:2663208/1‑49 (MQ=255) ttttGTTCGCAGAAGCGGAAAAATTCCTCTTCGCTCAATGTTTCACCtt > 1:1486895/1‑49 (MQ=255) ttttGTTCGCAGAAGCGGAAAAATTCCTCTTCGCTCAATGTTTCACCtt > 1:557297/1‑49 (MQ=255) ttttGTTCGCAGAAGCGGAAAAATTCCTCTTCGCTCAATGTTTCACCtt > 1:420258/1‑49 (MQ=255) ttttGTTCGCAGAAGCGGAAAAATTCCTCTTCGCTCAATGTTTCACCtt > 1:2895117/1‑49 (MQ=255) ttttGTTCGCAGAAGCGGAAAAATTCCTCTTCGCTCAATGTTTCACCTTCATTCAGCACCACAAATGCt > 1:547/1‑69 (MQ=255) ttttGTTCGCAGAAGCGGAAAAATTCCTCTTCGCTCAATGTTTCACCTTCATTCAGCACCACAAATGCt > 1:446581/1‑69 (MQ=255) ttttGTTCGCAGAAGCGGAAAAATTCCTCTTCGCTCAATGTTTCACCTTCATTCAGCACCACAAATGCt > 1:17991/1‑69 (MQ=255) ttttGTTCGCAGAAGCGGAAAAATTCCTCTTCGCTCAATGTTTCACCTTCATTCAGCACCACAAATGCt > 1:984811/1‑69 (MQ=255) ttttGTTCGCAGAAGCGGAAAAATTCCTCTTCGCTCAATGTTTCACATTCATTCAGCACCACAAATGCt > 1:748321/1‑69 (MQ=255) ttGTTCGCAGAAGCGGAAAAATTCCTCTTCGCTCAATGTTTCACCTTCATTCAGCACcaca > 1:730160/1‑61 (MQ=255) | CTTTTCTGATCTCCAGATAAGAGGGCACTTTAAATTTCGCCATATTTTGTTCGCAGAAGCGGAAAAATTCCTCTTCGCTCAATGTTTCACCTTCATTCAGCACCACAAATGCT > minE/33999‑34111 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |