| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 304,408 | T→A | 32.6% | P187P (CCT→CCA) | ybaY → | predicted outer membrane lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 304,408 | 0 | T | A | 32.6% | 48.0 / 33.8 | 49 | P187P (CCT→CCA) | ybaY | predicted outer membrane lipoprotein |
| Reads supporting (aligned to +/- strand): ref base T (10/23); new base A (11/5); total (21/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.51e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.43e-01 | |||||||||||
CAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCA > minE/304344‑304476 | cAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGc < 1:2037837/69‑1 (MQ=255)cAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGc < 1:502388/69‑1 (MQ=255)cAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGc < 1:2836494/69‑1 (MQ=255)cAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGc < 1:3300741/69‑1 (MQ=255)cAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGc < 1:1789134/69‑1 (MQ=255)cAACGACTACCGTACCTTCGACTTCACCAACTCAGGGGAATCCGTCTTCGGCAGTTCCAGCTCCTACGc < 1:1446429/69‑1 (MQ=255) aCGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCaa > 1:905994/1‑69 (MQ=255) aCGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCaa > 1:3268499/1‑69 (MQ=255) aCGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCaa > 1:1421706/1‑69 (MQ=255) ccTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGa > 1:3206360/1‑67 (MQ=255) ccTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGa > 1:2106871/1‑67 (MQ=255) ccTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGa > 1:2889978/1‑67 (MQ=255) ccTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGAc > 1:642791/1‑68 (MQ=255) aCTTCACCAACCCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCAACGCAATATTAAGCGAAGTTaa < 1:2229039/67‑1 (MQ=255) aCTTCACCAACCCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCAACGCAATATTAAGCGAAGTTaa < 1:1836926/67‑1 (MQ=255) aCTTCACCAACCCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCAACGCAATATTAAGCGAAGTTaa < 1:2888222/67‑1 (MQ=255) aCTTCACCAACCCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCAACGCAATATTAAGCGAAGTTaa < 1:2101113/67‑1 (MQ=255) gTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGga < 1:2275127/67‑1 (MQ=255) gTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGga < 1:1102567/67‑1 (MQ=255) gTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGga < 1:1952668/67‑1 (MQ=255) gTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGga < 1:1785254/67‑1 (MQ=255) gTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGga < 1:91973/67‑1 (MQ=255) gTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGga < 1:1168919/67‑1 (MQ=255) aTCCGTCTTCGGCAGTTCCAGCTCCAACGCAATATTAAGCGAAGTTaa < 1:2490006/48‑1 (MQ=255) tCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTaa > 1:350223/1‑47 (MQ=255) tCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCg > 1:563775/1‑60 (MQ=255) tCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCg > 1:2922158/1‑60 (MQ=255) gTCTTCGGCAGTTCCAGCTCCAACGCAATATTAAGCg > 1:423436/1‑37 (MQ=255) gTCTTCGGCAGTTCCAGCTCCAACGCAATATTAAGCg > 1:1581327/1‑37 (MQ=255) gTCTTCGGCAGTTCCAGCTCCAACGCAATATTAAGCg > 1:1169175/1‑37 (MQ=255) gTCTTCGGCAGTTCCAGCTCCAACGCAATATTAAGCGAAGt > 1:2647194/1‑41 (MQ=38) gTCTTCGGCAGTTCCAGCTCCAACGCAATATTAAGCGAAGTTAACCCTCTCCGCCCGGAGAGGGTtagt > 1:821594/1‑69 (MQ=39) gTCTTCGGCAGTTCCAGCTCCAACGCAATATTAAGCGAAGTTAACCCTCTCCGCCCGGAGAGGGTtagt > 1:2509420/1‑69 (MQ=39) gTCTTCGGCAGTTCCAGCTCCAACGCAATATTAAGCGAAGTTAACCCTCTCCGCCCGGAGAGGGTtagt > 1:2931083/1‑69 (MQ=39) gTCTTCGGCAGTTCCAGCTCCAACGCAATATTAAGCGAAGTTAACCCTCTCCGCCCGGAGAGGGTtagt > 1:1317365/1‑69 (MQ=39) gTCTTCGGCAGTTCCAGCTCCAACGCAATATTAAGCGAAGTTAACCCTCTCCGCCCGGAGAGGGTtagt > 1:2535327/1‑69 (MQ=39) gTCTTCGGCAGTTCCAGCTCCAACGCAATATTAAGCGAAGTTAACCCTCTCCGCCCGGAGAGGGTtagt > 1:1907846/1‑69 (MQ=39) gTCTTCGGCAGTTCCAGCTCCAACGCAATATTAAGCGAAGTTAACCCTCTCCGCCCGGAGAGGGTtagt > 1:239282/1‑69 (MQ=39) tCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTtagt < 1:3010772/68‑1 (MQ=255) tCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTtagt < 1:1193829/68‑1 (MQ=255) tCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTtagt < 1:787635/68‑1 (MQ=255) tCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTtagt < 1:461188/68‑1 (MQ=255) tCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTtagt < 1:2412281/68‑1 (MQ=255) tCTTCGGCAGGTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTtagt < 1:3250199/68‑1 (MQ=255) tCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGAt < 1:1713562/69‑1 (MQ=255) tCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGAt < 1:1616885/69‑1 (MQ=255) tCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGAt < 1:1012051/69‑1 (MQ=255) tCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGAt < 1:2896846/69‑1 (MQ=255) tCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGAt < 1:950496/69‑1 (MQ=255) tACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCa > 1:1240375/1‑69 (MQ=255) | CAACGACTACCGTACCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCA > minE/304344‑304476 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |