Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | minE | 304,425 | C→A | 31.7% | intergenic (+5/+26) | ybaY → / ← ybaZ | predicted outer membrane lipoprotein/predicted methyltransferase |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | minE | 304,425 | 0 | C | A | 31.7% | 48.1 / 23.0 | 41 | intergenic (+5/+26) | ybaY/ybaZ | predicted outer membrane lipoprotein/predicted methyltransferase |
Reads supporting (aligned to +/- strand): ref base C (6/22); new base A (8/5); total (14/27) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.71e-02 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCG > minE/304358‑304481 | ccTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGAc > 1:642791/1‑68 (MQ=255) aCTTCACCAACCCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCAACGCAATATTAAGCGAAGTTaa < 1:2888222/67‑1 (MQ=255) aCTTCACCAACCCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCAACGCAATATTAAGCGAAGTTaa < 1:2229039/67‑1 (MQ=255) aCTTCACCAACCCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCAACGCAATATTAAGCGAAGTTaa < 1:2101113/67‑1 (MQ=255) aCTTCACCAACCCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCAACGCAATATTAAGCGAAGTTaa < 1:1836926/67‑1 (MQ=255) gTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGga < 1:1102567/67‑1 (MQ=255) gTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGga < 1:1168919/67‑1 (MQ=255) gTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGga < 1:91973/67‑1 (MQ=255) gTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGga < 1:2275127/67‑1 (MQ=255) gTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGga < 1:1952668/67‑1 (MQ=255) gTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGga < 1:1785254/67‑1 (MQ=255) aTCCGTCTTCGGCAGTTCCAGCTCCAACGCAATATTAAGCGAAGTTaa < 1:2490006/48‑1 (MQ=255) tCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTaa > 1:350223/1‑47 (MQ=255) tCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCg > 1:563775/1‑60 (MQ=255) tCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCg > 1:2922158/1‑60 (MQ=255) gTCTTCGGCAGTTCCAGCTCCAACGCAATATTAAGCGAAGt > 1:2647194/1‑41 (MQ=38) gTCTTCGGCAGTTCCAGCTCCAACGCAATATTAAGCGAAGTTAACCCTCTCCGCCCGGAGAGGGTtagt > 1:1907846/1‑69 (MQ=39) gTCTTCGGCAGTTCCAGCTCCAACGCAATATTAAGCGAAGTTAACCCTCTCCGCCCGGAGAGGGTtagt > 1:821594/1‑69 (MQ=39) gTCTTCGGCAGTTCCAGCTCCAACGCAATATTAAGCGAAGTTAACCCTCTCCGCCCGGAGAGGGTtagt > 1:2931083/1‑69 (MQ=39) gTCTTCGGCAGTTCCAGCTCCAACGCAATATTAAGCGAAGTTAACCCTCTCCGCCCGGAGAGGGTtagt > 1:2535327/1‑69 (MQ=39) gTCTTCGGCAGTTCCAGCTCCAACGCAATATTAAGCGAAGTTAACCCTCTCCGCCCGGAGAGGGTtagt > 1:2509420/1‑69 (MQ=39) gTCTTCGGCAGTTCCAGCTCCAACGCAATATTAAGCGAAGTTAACCCTCTCCGCCCGGAGAGGGTtagt > 1:239282/1‑69 (MQ=39) gTCTTCGGCAGTTCCAGCTCCAACGCAATATTAAGCGAAGTTAACCCTCTCCGCCCGGAGAGGGTtagt > 1:1317365/1‑69 (MQ=39) tCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTtagt < 1:2412281/68‑1 (MQ=255) tCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTtagt < 1:787635/68‑1 (MQ=255) tCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTtagt < 1:1193829/68‑1 (MQ=255) tCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTtagt < 1:3010772/68‑1 (MQ=255) tCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTtagt < 1:461188/68‑1 (MQ=255) tCTTCGGCAGGTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTtagt < 1:3250199/68‑1 (MQ=255) tCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGAt < 1:950496/69‑1 (MQ=255) tCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGAt < 1:1012051/69‑1 (MQ=255) tCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGAt < 1:1713562/69‑1 (MQ=255) tCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGAt < 1:1616885/69‑1 (MQ=255) tCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGAt < 1:2896846/69‑1 (MQ=255) tACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCa > 1:1240375/1‑69 (MQ=255) aaTATTAAGCGACGTTAACCCTCTCCTCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCg < 1:1525333/69‑1 (MQ=255) aaTATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCg < 1:388720/69‑1 (MQ=255) aaTATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCg < 1:191/69‑1 (MQ=255) aaTATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCg < 1:133256/69‑1 (MQ=255) aaTATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCg < 1:1190202/69‑1 (MQ=255) ttAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAAc > 1:3425133/1‑54 (MQ=255) ttAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAAc > 1:3271880/1‑54 (MQ=255) | CCTTCGACTTCACCAACTCAGGTGAATCCGTCTTCGGCAGTTCCAGCTCCTACGCAATATTAAGCGACGTTAACCCTCTCCGCCCGGAGAGGGTTAGTAGTTCCAGCGATAACGCTGCAAGTCG > minE/304358‑304481 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |