| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 305,631 | A→G | 29.3% | L486L (TTA→CTA) | ylaB ← | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 305,631 | 0 | A | G | 29.3% | 46.4 / 20.8 | 51 | L486L (TTA→CTA) | ylaB | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base A (20/16); new base G (8/7); total (28/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.22e-01 | |||||||||||
CTTCTTTCGGTAATGCCTTGCTGTAGAGCCAGCCCTGACCGTAGTGCACGCCATGCTGGCGTAACCACTCTTCTTGTTTACTGGTTTCGATTCCCTCCGCTACCATTTTCAGTTTCAGTGTTTTTGC > minE/305568‑305694 | cttcttTCGGTAATGCCTTGCTGTAGAGCCAGCCCTGACCGTAGTGCACGCCATGCTGGCGTAACCAct < 1:2166835/69‑1 (MQ=255) tAATGCCTTGCTGTAGAGCCAGCCCTGACCGTAGTGCACGCCATGCTGGCGTAACCACTCTTCTTGtt < 1:841474/68‑1 (MQ=255) tAATGCCTTGCTGTAGAGCCAGCCCTGACCGTAGTGCACGCCATGCTGGCGTAACCACTCTTCTTGtt < 1:487198/68‑1 (MQ=255) tAATGCCTTGCTGTAGAGCCAGCCCTGACCGTAGTGCACGCCATGCTGGCGTAACCACTCTTCTTGtt < 1:1831614/68‑1 (MQ=255) tAATGCCTTGCTGTAGAGCCAGCCCTGACCGTAGTGCACGCCATGCTGGCGTAACCACTCTTCTTGtt < 1:1768467/68‑1 (MQ=255) aTGCCTTGCTGTAGAGCCAGCCCTGACCGTAGTGCACGCCATGCTGGCGTAACCACTCTTCTTGtt < 1:1282332/66‑1 (MQ=255) gCCTTGCTGTAGAGCCAGCCCTGACCGTAGTGCACGCCATGCTGGCGTAACCACTCTTCTTGTTTACt < 1:53931/68‑1 (MQ=255) gagCCAGCCCTGACCTTAGTGCACGCCATGCTGGCGTAACCACTCTTCTTGTTTGCTGGTTTCGATTcc > 1:652243/1‑69 (MQ=255) gagCCAGCCCTGACCGTAGTGCACGCCATGCTGGCGTAACCACTCTTCTTg > 1:603843/1‑51 (MQ=255) gagCCAGCCCTGACCGTAGTGCACGCCATGCTGGCGTAACCACTCTTCTTGTTTACTGGTTTCGATTc > 1:2138773/1‑68 (MQ=255) cTGACCGTATTGCACGCCATGCTGGCGTAGCCACTCTTCTTg > 1:3148729/1‑42 (MQ=255) cTGACCGTAGTGCACGCCATGCTGGCGTAGCCCCtcttc > 1:1773094/1‑39 (MQ=38) cTGACCGTAGTGCACGCCATGCTGGCGTAGCCACTCTTCTTg > 1:2924216/1‑42 (MQ=255) cTGACCGTAGTGCACGCCATGCTGGCGTAGCCACTCTTCTTg > 1:2966415/1‑42 (MQ=255) cTGACCGTAGTGCACGCCATGCTGGCGTAGCCACTCTTCTTg > 1:2912347/1‑42 (MQ=255) cTGACCGTAGTGCACGCCATGCTGGCGTAGCCACTCTTCTTg > 1:2161522/1‑42 (MQ=255) cTGACCGTAGTGCACGCCATGCTGGCGTAGCCACTCTTCTTg > 1:1220124/1‑42 (MQ=255) cTGACCGTAGTGCACGCCATGCTGGCGTAGCCACTCTTCTTg > 1:1918202/1‑42 (MQ=255) cTGACCGTAGTGCACGCCATGCTGGCGTAACCACTCTTCTTGTTTACTGGTTTCGATTCCCTCCGCTAc < 1:1980855/69‑1 (MQ=255) cTGACCGTAGTGCACGCCATGCTGGCGTAACCACTCTTCTTGTTTACTGGTTTCGATTCCCTCCGCTAc < 1:3235584/69‑1 (MQ=255) cTGACCGTAGTGCACGCCATGCTGGCGTAACCACTCTTCTTGTTTACTGGTTTCGATTCCCTCCGCTAc < 1:1507032/69‑1 (MQ=255) cTGACCGTAGTGCACGCCATGCTGGCGTAACCACTCTTCTTGTTTACTGGTTTCGATTCCCTCCGCTAc < 1:1735384/69‑1 (MQ=255) tGACCGTAGTGCACGCCATGCTGGCGTAACCACTCTTCTTGTTTACTGGTTTCGATTCCCTCCGCTAcc < 1:3422195/69‑1 (MQ=255) tGACCGTAGTGCACGCCATGCTGGCGTAACCACTCTTCTTGTTTACTGGTTTCGATTCCCTCCGCTAcc < 1:3231669/69‑1 (MQ=255) cATGCTGGCGTAACCACTCTTCTTGTTTACTGGTTTCGATTCCCTCCGCTACCATTTTCAGTTTCAgtg > 1:1497452/1‑69 (MQ=255) cATGCTGGCGTAACCACTCTTCTTGTTTACTGGTTTCGATTCCCTCCGCTACCATTTTCAGTTTCAgtg > 1:1142618/1‑69 (MQ=255) cATGCTGGCGTAACCACTCTTCTTGTTTACTGGTTTCGATTCCCTCCGCTACCATTTTCAGTTTCAgtg > 1:689883/1‑69 (MQ=255) cATGCTGGCGTAACCACTCTTCTTGTTTACTGGTTTCGATTCCCTCCGCTACCATTTTCAGTTTCAgtg > 1:665913/1‑69 (MQ=255) cATGCTGGCGTAACCACTCTTCTTGTTTACTGGTTTCGATTCCCTCCGCTACCATTTTCAGTTTCAgtg > 1:1254055/1‑69 (MQ=255) cATGCTGGCGTAACCACTCTTCTTGTTTACTGGTTTCGATTCCCTCCGCTACCATTTTCAGTTTCAgtg > 1:479851/1‑69 (MQ=255) cATGCTGGCGTAACCACTCTTCTTGTTTACTGGTTTCGATTCCCTCCGCTACCATTTTCAGTTTCAgtg > 1:2722562/1‑69 (MQ=255) cATGCTGGCGTAACCACTCTTCTTGTTTACTGGTTTCGATTCCCTCCGCTACCATTTTCAGTTTCAgtg > 1:2479950/1‑69 (MQ=255) cATGCTGGCGTAACCACTCTTCTTGTTTACTGGTTTCGATTCCCTCCGCTACCATTTTCAGTTTCAgtg > 1:1993129/1‑69 (MQ=255) cATGCTGGCGTAACCACTCTTCTTGTTTACTGGTTTCGATTCCCTCCGCTACCATTTTCAGTTTCAgtg > 1:2222332/1‑69 (MQ=255) cATGCTGGCGTAACCACTCTTCTTGTTTACTGGATTCGATTCCCTCCGCTACCATTTTCAGTTTCAgtg > 1:345308/1‑69 (MQ=255) cATGCTGGCGTAACCACTCTTCTTGTATACTGGTTTCGATTCCCTCCGCTACCATtttcagtttcag > 1:3191071/1‑67 (MQ=255) cTGGCGTAACCACTCTTCTTGTTTACTGGTTTCGATTCCCTCCGCTAc < 1:2589240/48‑1 (MQ=255) cTGGCGTAACCACTCTTCTTGTTTACTGGTTTCGATTCCCTCCGCTACCATTTTCAGTTTCAGTGtttt > 1:2616234/1‑69 (MQ=255) cTGGCGTAACCACTCTTCTTGTTTACTGGTTTCGATTCCCTCCGCTACCATTTTCAGTTTCAGTGtttt > 1:2102049/1‑69 (MQ=255) cTGGCGTAACCACTCTTCTTGTTTACTGGTTTCGATTCCCTCCGCTACCATTTTCAGTTTCAGTGtttt > 1:2270609/1‑69 (MQ=255) cTGGCGTAACCACTCTTCTTGTTTACTGGTTTCGATTCCCTCCGCTACCATTTTCAGTTTCAGTGtttt > 1:472973/1‑69 (MQ=255) cTGGCGTAACCACTCTTCTTGTTTACTGGTTTCGATTCCCTCCGCTACCATTTTCAGTTTCAGTGtttt > 1:1412563/1‑69 (MQ=255) ggCGTAGCCACTCTTCTTGTTTACTGGTTTCGATTCCCTCCGCAACCATTTTCAGTTTCAGTGttttt < 1:2647797/68‑1 (MQ=255) ggCGTAGCCACTCTTCTTGTTTACTGGTTTCGATTCCCTCCGCAACCATTTTCAGTTTCAGTGttttt < 1:786875/68‑1 (MQ=255) ggCGTAGCCACTCTTCTTGTTTACTGGTTTCGATTCCCTCCGCAACCATTTTCAGTTTCAGTGttttt < 1:1947034/68‑1 (MQ=255) ggCGTAGCCACTCTTCTTGTTTACTGGTTTCGATTCCCTCCGCAACCATTTTCAGTTTCAGTGttttt < 1:1423618/68‑1 (MQ=255) ggCGTAGCCACTCTTCTTGTTTACTGGTTTCGATTCCCTCCGCAACCATTTTCAGTTTCAGTGttttt < 1:1484134/68‑1 (MQ=255) ggCGTAGCCACTCTTCTTGTTTACTGGTTTCGATTCCCTCCGCAACCATTTTCAGTTTCAGTGttttt < 1:3412856/68‑1 (MQ=255) ggCGTAGCCACTCTTCTTGTTTACTGGTTTCGATTCCCTCCGCAACCATTTTCAGTTTCAGTGttttt < 1:2643830/68‑1 (MQ=255) gTAACCACTCTTCTTGTTTACTGGTTTCGATTCCCTCCGCTACCATTTTCAGTTTCAGTGTTTTTGc < 1:657137/67‑1 (MQ=255) gTAACCACTCTTCTTGTTTACTGGTTTCGATTCCCTCCGCTACCATTTTCAGTTTCAGTGTTTTTGc < 1:2155236/67‑1 (MQ=255) aCCACTCTTCTTGTTTACTGGTTTCGATTCCCTCCGCTAc < 1:2116018/40‑1 (MQ=255) | CTTCTTTCGGTAATGCCTTGCTGTAGAGCCAGCCCTGACCGTAGTGCACGCCATGCTGGCGTAACCACTCTTCTTGTTTACTGGTTTCGATTCCCTCCGCTACCATTTTCAGTTTCAGTGTTTTTGC > minE/305568‑305694 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |