| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 306,979 | C→T | 44.9% | S36S (TCG→TCA) | ylaB ← | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 306,979 | 0 | C | T | 44.9% | 26.1 / 89.7 | 112 | S36S (TCG→TCA) | ylaB | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (51/10); new base T (22/28); total (74/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.76e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 113 total aligned reads displayed.
GCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGA > minE/306920‑307042 | gCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCt < 1:1975689/69‑1 (MQ=255)gCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCt < 1:1412307/69‑1 (MQ=255)gCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCt < 1:134379/69‑1 (MQ=255) tATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACctgct < 1:1655590/69‑1 (MQ=255) ggCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAAc > 1:1874840/1‑49 (MQ=255) ggCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATg > 1:1856114/1‑69 (MQ=255) ggCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATg > 1:1450650/1‑69 (MQ=255) ggCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATg > 1:1319121/1‑69 (MQ=255) ggCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATg > 1:1268020/1‑69 (MQ=255) ggCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATg > 1:3181608/1‑69 (MQ=255) ggCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATg > 1:2825157/1‑69 (MQ=255) ggCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATg > 1:1169488/1‑69 (MQ=255) ggCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATg > 1:1125658/1‑69 (MQ=255) aTAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGgcca > 1:3145580/1‑67 (MQ=255) aTAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGgcca > 1:3060428/1‑67 (MQ=255) tAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATAAATGATGtt > 1:1108513/1‑47 (MQ=255) tAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATAAATGATGTTTCTa > 1:2776064/1‑51 (MQ=255) tAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATAAATGATGTTTCTACctgct > 1:2626319/1‑57 (MQ=255) tAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATAAATGATGTTTCTACctgct > 1:3075793/1‑57 (MQ=255) tAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATAAATGATGTTTCTACctgct > 1:1962218/1‑57 (MQ=255) tAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATAAATGATGTTTCTACctgct > 1:3126207/1‑57 (MQ=255) tAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATAAATGATGTTTCTACctgct > 1:425435/1‑57 (MQ=255) tAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATAAATGATGTTTCTACctgct > 1:1557534/1‑57 (MQ=255) tAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATAAATGATGTTTCTACctgct > 1:220862/1‑57 (MQ=255) tAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATAAATGATGTTTCTACctgct > 1:2436536/1‑57 (MQ=255) tAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATAAATGATGTTTCTACctgct > 1:331342/1‑57 (MQ=255) tAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATAAATGATGTTTCTACctgct > 1:282594/1‑57 (MQ=255) tAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATAAATGATGTTTCTACctgct > 1:1394978/1‑57 (MQ=255) tAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATAAATGATGTTTCTACctgct > 1:137626/1‑57 (MQ=255) tAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATAAATGATGTTTCTACctgct > 1:1136801/1‑57 (MQ=255) tAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATAAATGATGTTTCTACctgct > 1:273325/1‑57 (MQ=255) tAGCGACGCGGGAGGAATACGTATTCAGCTCTTCAATAAATGATGTTTCTACctgc > 1:3132442/1‑56 (MQ=255) tAGCGACGCGGGAGGAATACGGATTCAGCTCTTCAATAAATGATGTTTCTACctgct > 1:452503/1‑57 (MQ=255) tAGCGACCCGGGAGGAATACGTATTCAGCTCTTCAATAAATGATGTTTCTACctgct > 1:3263567/1‑57 (MQ=255) ggaggaATAGGTATCCAGCTCTTCAATAAACGATGTTTCt < 1:1026369/40‑1 (MQ=255) ggTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGCCGACa > 1:536000/1‑67 (MQ=255) ggTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGCCGACa > 1:1714031/1‑67 (MQ=255) ggTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGCCGACa > 1:3397633/1‑67 (MQ=255) ggTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGCCGACa > 1:2419611/1‑67 (MQ=255) cgTATCCAGCTCTTCAATAAAAGATGTTTCTACATGCTGATGGGCCAGCCAGATGCTTAAGc > 1:1508340/2‑62 (MQ=255) cAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGCCGACAGTCAGCaa < 1:4931/69‑1 (MQ=255) cAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGCCGACAGGCAGCaa > 1:422630/1‑69 (MQ=255) cAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGCCGACAGGCAGCaa < 1:1176115/69‑1 (MQ=255) cAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGCCGACAGGCAGCaa > 1:1177995/1‑69 (MQ=255) cAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGCCGACAGGCAGCaa > 1:1206465/1‑69 (MQ=255) cAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGCCGACAGGCAGCaa > 1:1209566/1‑69 (MQ=255) cAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGCCGACAGGCAGCaa > 1:1817579/1‑69 (MQ=255) cAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGCCGACAGGCAGCaa > 1:424092/1‑69 (MQ=255) gCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGCCGACAGGCAGCAATa < 1:1532654/69‑1 (MQ=255) gCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGCCGACAGGCAGCAATa < 1:174803/69‑1 (MQ=255) ctTCAATAAATGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTg < 1:1090493/69‑1 (MQ=255) ctTCAATAAATGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTg < 1:890284/69‑1 (MQ=255) ctTCAATAAATGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTg < 1:2999875/69‑1 (MQ=255) ctTCAATAAATGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTg < 1:2993093/69‑1 (MQ=255) ctTCAATAAATGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTg < 1:288072/69‑1 (MQ=255) ctTCAATAAATGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTg < 1:1180441/69‑1 (MQ=255) ctTCAATAAATGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTg < 1:3059188/69‑1 (MQ=255) ctTCAATAAATGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTg < 1:2731575/69‑1 (MQ=255) ctTCAATAAATGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTg < 1:2348080/69‑1 (MQ=255) ctTCAATAAATGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTg < 1:355944/69‑1 (MQ=255) ctTCAATAAATGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTg < 1:3418372/69‑1 (MQ=255) ctTCAATAAATGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTg < 1:2245543/69‑1 (MQ=255) ctTCAATAAATGAAGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTg < 1:1053887/69‑1 (MQ=255) ttCAATAAATGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTg < 1:196099/68‑1 (MQ=255) ttCAATAAATGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTg < 1:2619669/68‑1 (MQ=255) ttCAATAAATGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTg < 1:471502/68‑1 (MQ=255) cAATAAACGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGaa > 1:976085/1‑68 (MQ=255) cAATAAACGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGaa > 1:360600/1‑68 (MQ=255) cAATAAACGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGaa > 1:527861/1‑68 (MQ=255) cAATAAACGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGaa > 1:357580/1‑68 (MQ=255) cAATAAACGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGaa > 1:1359793/1‑68 (MQ=255) cAATAAACGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGaa > 1:1523052/1‑68 (MQ=255) cAATAAACGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGaa > 1:1575495/1‑68 (MQ=255) cAATAAACGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGaa > 1:278059/1‑68 (MQ=255) cAATAAACGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGaa > 1:1751408/1‑68 (MQ=255) cAATAAACGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGaa > 1:2624757/1‑68 (MQ=255) cAATAAACGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGaa > 1:1775240/1‑68 (MQ=255) cAATAAACGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGaa > 1:2012464/1‑68 (MQ=255) cAATAAACGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGa > 1:319495/1‑67 (MQ=255) cAATAAACGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGa > 1:1921927/1‑67 (MQ=255) tAAATGATGTTTCTTCCTGCTGATGGGCCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGa < 1:1005540/68‑1 (MQ=255) tAAATGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGa < 1:3424141/68‑1 (MQ=255) tAAATGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGa < 1:938955/68‑1 (MQ=255) tAAATGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGa < 1:3407306/68‑1 (MQ=255) tAAATGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGa < 1:2552089/68‑1 (MQ=255) tAAATGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGa < 1:928815/68‑1 (MQ=255) tAAATGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGa < 1:724894/68‑1 (MQ=255) tAAATGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGa < 1:1541821/68‑1 (MQ=255) tAAATGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGa < 1:3014733/68‑1 (MQ=255) tAAATGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGa < 1:3027938/68‑1 (MQ=255) tAAATGATGTTTCTACCTGCCGATGGGCCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGa < 1:1122680/68‑1 (MQ=255) tAAACGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAgac > 1:2021875/1‑44 (MQ=255) tAAACGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGcc > 1:1295599/1‑46 (MQ=255) tAAACGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGcc > 1:2921259/1‑46 (MQ=255) tAAACGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGcc > 1:164033/1‑46 (MQ=255) tAAACGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGcc > 1:2712028/1‑46 (MQ=255) tAAACGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGcc > 1:2539493/1‑46 (MQ=255) tAAACGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGcc > 1:963341/1‑46 (MQ=255) tAAACGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGcc > 1:967280/1‑46 (MQ=255) tAAACGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGcc > 1:3066994/1‑46 (MQ=255) | GCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGA > minE/306920‑307042 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |