Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 307,965 G→A 47.9% R141R (CGC→CGT maa ← maltose O‑acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE307,9650GA47.9% 11.5 / 108.2 123R141R (CGC→CGTmaamaltose O‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base G (44/20);  new base A (14/45);  total (58/65)
Fisher's exact test for biased strand distribution p-value = 7.28e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01

Only 100 of 136 total aligned reads displayed.

AACTGCACCTGAGGCTACCACGACGTTATCACCAATGGTCACACCAGGGTTAATGACCGCGCGTCCGCCAATCCAGACGTTATTACCGATGGTGACGGGTTTCCCCAGTTCAGCACCGCTATTA  >  minE/307905‑308028
                                                            |                                                               
aaCTGCACCTTAGGCTACCACGACGTTATCACCAATGGTCACACCAGGGTTAATGACCGCGCGTCCGcc                                                         >  1:1396640/1‑69 (MQ=255)
aaCTGCACCTGAGGCTACCACGACGTTATCGCCAATGGTCACACCAGGGTTAATGACCGCGCGTCCGc                                                          >  1:2487505/1‑68 (MQ=255)
aaCTGCACCTGAGGCTACCACGACGTTATCACCAATGGTCACACCAGGGTTAATGACCGCGCGTCCGcc                                                         >  1:2816267/1‑69 (MQ=255)
aaCTGCACCTGAGGCTACCACGACGTTATCACCAATGGTCACACCAGGGTTAATGACCGCGCGTCCGcc                                                         >  1:478352/1‑69 (MQ=255)
aaCTGCACCTGAGGCTACCACGACGTTATCACCAATGGTCACACCAGGGTTAATGACCGCGCGTCCGcc                                                         >  1:3130761/1‑69 (MQ=255)
aaCTGCACCTGAGGCTACCACGACGTTATCACCAATGGTCACACCAGGGTTAATGACCGCGCGTCCGc                                                          >  1:794126/1‑68 (MQ=255)
aaCTGCACCTGAGGCTACCACGACGTTATCACCAATGGTCACACCAGGGTTAATGACCGCGCGTCCGc                                                          >  1:1583510/1‑68 (MQ=255)
   tGCACCTGAGGCTACCACGACGTTATCACAATGGTCACACCAGGGTTAATGACTGCACGTCCGCCAATc                                                     <  1:1180354/69‑1 (MQ=255)
    gCACCTGAGGCTACCACGACGTTATCACCAATGGTCACACCAGGGTTAATGTCTGCACGTCCGCCAATc                                                     <  1:1358669/69‑1 (MQ=255)
    gCACCTGAGGCTACCACGACGTTATCACCAATGGTCACACCAGGGTTAATGACTGCACGTCCGCCAATc                                                     <  1:1057894/69‑1 (MQ=255)
    gCACCTGAGGCTACCACGACGTTATCACCAATGGTCACACCAGGGTTAATGACTGCACGTCCGCCAATc                                                     <  1:2730075/69‑1 (MQ=255)
    gCACCTGAGGCTACCACGACGTTATCACCAATGGTCACACCAGGGTTAATGACTGCACGTCCGCCAATc                                                     <  1:1528231/69‑1 (MQ=255)
    gCACCTGAGGCTACCACGACGTTATCACCAATGGTCACACCAGGGTTAATGACTGCACGTCCGCCAATc                                                     <  1:2893913/69‑1 (MQ=255)
    gCACCTGAGGCTACCACGACGTTATCACCAATGGTCACACCAGGGTTAATGACTGCACGTCCGCCAATc                                                     <  1:3217135/69‑1 (MQ=255)
    gCACCTGAGGCTACCACGACGTTATCACCAATGGTCACACCAGGGTTAATGACTGCACGTCCGCCAATc                                                     <  1:2594511/69‑1 (MQ=255)
    gCACCTGAGGCTACCACGACGTTATCACCAATGGTCACACCAGGGTTAATGACTGCACGTCCGCCAATc                                                     <  1:2495089/69‑1 (MQ=255)
    gCACCTGAGGCTACCACGACGTTATCACCAATGGTCACACCAGGGTTAATGACTGCACGTCCGCCAATc                                                     <  1:32495/69‑1 (MQ=255)
    gCACCTGAGGCTACCACGACGTTATCACCAATGGTCACACCAGGGTTAATGACTGCACGTCCGCCAATc                                                     <  1:1429302/69‑1 (MQ=255)
    gCACCTGAGGCTACCACGACGTTATCACCAATGGTCACACCAGGGTTAATGACTGCACGTCCGCCAATc                                                     <  1:960902/69‑1 (MQ=255)
    gCACCTGAGGCTACCACGACGTTATCACCAATGGTCACACCAGGGTTAATGACTGCACGTCCGCCAATc                                                     <  1:1356600/69‑1 (MQ=255)
    gCACCTGAGGCTACCACGACGTTATCACCAATGGTCACACCAGGGTTAATGACTGCACGTCCGCCAATc                                                     <  1:50575/69‑1 (MQ=255)
    gCACCTGAGGCTACCACGACGTTATCACCAATGGTCACACCAGGGTTAATGACTGCACGTCCGCCAATc                                                     <  1:1300904/69‑1 (MQ=255)
    gCACCTGAGGCTACCACGACGTTATCACCAATGGTCACACCAGGGTTAATGACTGCACGTCCGCCAATc                                                     <  1:1160343/69‑1 (MQ=255)
    gCACCTGAGGCTACCACGACGTTATCACCAATGGTCACACCAGGGTTAATGACTGCACGTCCGCCAATc                                                     <  1:748833/69‑1 (MQ=255)
    gCACCTGAGGCTACCAAGACGTTATCACCAATGGTCACACCAGGGTTAATGACTGCACGTCCGCCAATc                                                     <  1:615030/69‑1 (MQ=255)
     cACCTGAGGCTACCACGACGTTATCACCAATGGTCACACCAGGGTTAATGACTGCACGTCCGCCAATc                                                     <  1:2580103/68‑1 (MQ=255)
     cACCTGAGGCTACCACGACGTTATCACCAATGGTCACACCAGGGTTAATGACTGCACGTCCGCCAATc                                                     <  1:3069922/68‑1 (MQ=255)
     cACCTGAGGCTACCACGACGTTATCACCAATGGTCACACCAGGGTTAATGACTGCACGTCCGCCAATc                                                     <  1:1817965/68‑1 (MQ=255)
          gAGGCTACCACGACGTTATCACCAATGGTCACACCAGGGTTAATGACTGCACGTCCGCCAATc                                                     <  1:1909393/63‑1 (MQ=255)
              cTACCACGACGTTATCACCAATGGTCACACCAGGGTTAATGACCGCGCGTCCGCCAATCCAGACGt                                              <  1:554922/66‑1 (MQ=255)
               tACCACGACGTTATCACCAATGGTCACACCAGGGTTAATGACTGCACGTCCGCCAATCCAGAc                                                >  1:2756949/1‑63 (MQ=255)
               tACCACGACGTTATCACCAATGGTCACACCAGGGTTAATGACTGCACGTCCGCCAATCCAGACGttatt                                          >  1:1063424/1‑69 (MQ=255)
               tACCACGACGTTATCACCAATGGTCACACCAGGGTTAATGACTGCACGTCCGCCAATCCAGACGttatt                                          >  1:2616619/1‑69 (MQ=255)
               tACCACGACGTTATCACCAATGGTCACACCAGGGTTAATGACTGCACGTCCGCCAATCCAGACGttatt                                          >  1:1192833/1‑69 (MQ=255)
               tACCACGACGTTATCACCAATGGTCACACCAGGGTTAATGACTGCACGTCCGCCAATCCAGACGttatt                                          >  1:1983874/1‑69 (MQ=255)
               tACCACGACGTTATCACCAATGGTCACACCAGGGTTAATGACTGCACGTCCGCCAATCCAGACGttatt                                          >  1:2879929/1‑69 (MQ=255)
               tACCACGACGTTATCACCAATGGTCACACCAGGGTTAATGACTGCACGTCCGCCAATCCAGACGttatt                                          >  1:1401369/1‑69 (MQ=255)
               tACCACGACGTTATCACCAATGGTCACACCAGGGTTAATGACTGCACGTCCGCCAATCCAGACGttatt                                          >  1:3265198/1‑69 (MQ=255)
                   acgacgTTATCACCAATGGTCACACCAGGGTTAATGACCGCGCGTCCGCCAATCCAGACGTTATTAcc                                       <  1:266684/68‑1 (MQ=255)
                        gTTATCACCAATGGTCACACCAGGGTTAATGACCGCGCGTCCGCCAATCCAGACGTTATTACCGATGGt                                 <  1:2708756/69‑1 (MQ=255)
                           aTCACCAATGGTCACACCAGGGTTAATGACCGCGCGTCCGCCAATCGAGACGttatta                                         >  1:2937357/1‑58 (MQ=255)
                           aTCACCAATGGTCACACCAGGGTTAATGACCGCGCGTCCGCCAATCCAGACGTTATTACCGATGGTGAc                              >  1:898137/1‑69 (MQ=255)
                           aTCACCAATGGTCACACCAGGGTTAATGACCGCGCGTCCGCCAATCCAGACGTTATTACCGATGGTGAc                              >  1:1396243/1‑69 (MQ=255)
                           aTCACCAATGGTCACACCAGGGTTAATGACCGCGCGTCCGCCAATCCAGACGTTATTACCGATGGTGAc                              >  1:355724/1‑69 (MQ=255)
                           aTCACCAATGGTCACACCAGGGTTAATGACCGCGCGTCCGCCAATCCAGACGTTATTACCGATGGTGAc                              >  1:2216008/1‑69 (MQ=255)
                           aTCACCAATGGTCACACCAGGGTTAATGACCGCGCGTCCGCCAATCCAGACGTTATTACCGATGGTGAc                              >  1:1248615/1‑69 (MQ=255)
                           aTCACCAATGGTCACACCAGGGTTAATGACCGCGCGTCCGCCAATCCAGACGTTATTACCGATGGTGAc                              >  1:2625006/1‑69 (MQ=255)
                           aTCACCAATGGTCACACCAGGGTTAATGACCGCGCGTCCGCCAATCCAGACGTTATTACCGATGGTGAc                              >  1:2135048/1‑69 (MQ=255)
                           aTCACCAATGGTCACACCAGGGTTAATGACCGCGCGTCCGCCAATCCAGACGTTATTACCGATGGTGAc                              >  1:740502/1‑69 (MQ=255)
                                  aTGGTCACACCAGGGGTAATGACTGCACGTCCGCCAATc                                                     <  1:832893/39‑1 (MQ=37)
                                     gTCACACCAGGGTTAATGACCGCGCGTCCGCCAATCCAGACGtt                                             <  1:1289322/44‑1 (MQ=255)
                                     gTCACACCAGGGTTAATGACCGCGCGTCCGCCAATCCAGACGtt                                             <  1:2746825/44‑1 (MQ=255)
                                     gTCACACCAGGGTTAATGACCGCGCGTCCGCCAATCCAGACGtt                                             <  1:1800787/44‑1 (MQ=255)
                                     gTCACACCAGGGTTAATGACCGCGCGTCCGCCAATCCAGACGtt                                             <  1:1796976/44‑1 (MQ=255)
                                     gTCACACCAGGGTTAATGACCGCGCGTCCGCCAATCCAGACGtt                                             <  1:3382092/44‑1 (MQ=255)
                                     gTCACACCAGGGTTAATGACCGCGCGTCCGCCAATCCAGACGtt                                             <  1:3212079/44‑1 (MQ=255)
                                     gTCACACCAGGGTTAATGACCGCGCGTCCGCCAATCCAGACGtt                                             <  1:660291/44‑1 (MQ=255)
                                     gTCACACCAGGGTTAATGACCGCGCGTCCGCCAATCCAGACGtt                                             <  1:2232631/44‑1 (MQ=255)
                                     gTCACACCAGGGTTAATGACCGCGCGTCCGCCAATCCAGACGtt                                             <  1:248801/44‑1 (MQ=255)
                                     gTCACACCAGGGTTAATGACCGCGCGTCCGCCAATCCAGACGTTATTAcc                                       >  1:2837216/1‑50 (MQ=255)
                                     gTCACACCAGGGTTAATGACCGCGCGTCCGCCAATCCAGACGTTATTACCGATGGTGACGGGTTTcccc                    >  1:2752132/1‑69 (MQ=255)
                                     gTCACACCAGGGTTAATGACCGCGCGTCCGCCAATCCAGACGTTATTACCGATGGTGACGGGTTTcccc                    >  1:878908/1‑69 (MQ=255)
                                     gTCACACCAGGGTTAATGACCGCGCGTCCGCCAATCCAGACGTTATTACCGATGGTGACGGGTTTcccc                    >  1:1621464/1‑69 (MQ=255)
                                       cacaCCAGGGTTAATGACTGCACGTCCGCCAATCCAGACGt                                              <  1:1305368/41‑1 (MQ=255)
                                       cacaCCAGGGTTAATGACTGCACGTCCGCCAATCCAGACGt                                              <  1:969844/41‑1 (MQ=255)
                                       cacaCCAGGGTTAATGACTGCACGTCCGCCAATCCAGACGt                                              <  1:780223/41‑1 (MQ=255)
                                       cacaCCAGGGTTAATGACTGCACGTCCGCCAATCCAGACGt                                              <  1:1571172/41‑1 (MQ=255)
                                       cacaCCAGGGTTAATGACTGCACGTCCGCCAATCCAGACGt                                              <  1:2370796/41‑1 (MQ=255)
                                       cacaCCAGGGTTAATGACTGCACGTCCGCCAATCCAGACGt                                              <  1:2370952/41‑1 (MQ=255)
                                       cacaCCAGGGTTAATGACTGCACGTCCGCCAATCCAGACGt                                              <  1:2330826/41‑1 (MQ=255)
                                       cacaCCAGGGTTAATGACTGCACGTCCGCCAATCCAGACGt                                              <  1:1911331/41‑1 (MQ=255)
                                       cacaCCAGGGTTAATGACCGCGCGTCCGCCAATCCAGACGTTATTACCGATGGTGACGGGTTTCCCCAg                  <  1:1527227/69‑1 (MQ=255)
                                        acacCAGGGTTAATGACCGCGCGTCCGCCAATCCAGACGTTATTACCGATGGTGACGGGTTTCCCCAg                  >  1:1685476/1‑68 (MQ=255)
                                        acacCAGGGTTAATGACCGCGCGTCCGCCAATCCAGACGTTATTACCGATGGTGACGGGTTTCCCCAg                  >  1:1122163/1‑68 (MQ=255)
                                        acacCAGGGTTAATGACCGCGCGTCCGCCAATCCAGACGTTATTACCGATGGTGACGGGTTTCCCCAg                  >  1:2318558/1‑68 (MQ=255)
                                        acacCAGGGTTAATGACCGCGCGTCCGCCAATCCAGACGTTATTACCGATGGTGACGGGTTTCCCCAg                  >  1:2207569/1‑68 (MQ=255)
                                        acacCAGGGTTAATGACCGCGCGTCCGCCAATCCAGACGTTATTACCGATGGTGACGGGTTTCCCCAg                  >  1:1264682/1‑68 (MQ=255)
                                        acacCAGGGTTAATGACCGCGCGTCCGCCAATCCAGACGTTATTACCGATGGTGACGGGTTTCCCCAg                  >  1:281283/1‑68 (MQ=255)
                                        acacCAGGGTTAATGACCGCGCGTCCGCCAATCCAGACGTTATTACCGATGGTGACGGGTTTCCCCAg                  >  1:1869107/1‑68 (MQ=255)
                                          acCAGGGTTAATGACCGCGCGTCCGCCAATCCAGACGTTATTACCGATgg                                  >  1:318767/1‑50 (MQ=255)
                                          acCAGGGTTAATGACCGCGCGTCCGCCAATCCAGACGTTATTACCGATGGTGACGGGTTTCCCCAGTTc               >  1:3022294/1‑69 (MQ=255)
                                          acCAGGGTTAATGACCGCGCGTCCGCCAATCCAGACGTTATTACCGATGGTGACGGGTTTCCCCAGTTc               >  1:1281313/1‑69 (MQ=255)
                                              gggTTAATGACCGCGCGTCCGCCAATCCAGACGtt                                             <  1:238958/35‑1 (MQ=255)
                                              gggTTAATGACCGCGCGTCCGCCAATCCAGACGTTATTACCGATGGTGACGGGTTTCCCCAGTTCAGCa           >  1:2595318/1‑69 (MQ=255)
                                              gggTTAATGACCGCGCGTCCGCCAATCCAGACGTTATTACCGATGGTGACGGGTTTCCCCAGTTCAGCa           >  1:490299/1‑69 (MQ=255)
                                                gTTAATGACCGCGCGTCCGCCAATCCAGACGTTATTACCGATGGTGACGGGTTTCCCCAg                  >  1:2032943/1‑60 (MQ=255)
                                                gTTAATGACCGCGCGTCCGCCAATCCAGACGTTATTACCGATGGTGACGGGTTTCCCCAg                  >  1:810501/1‑60 (MQ=255)
                                                gTTAATGACCGCGCGTCCGCCAATCCAGACGTTATTACCGATGGTGACGGGTTTCCCCAg                  >  1:587550/1‑60 (MQ=255)
                                                gTTAATGACCGCGCGTCCGCCAATCCAGACGTTATTACCGATGGTGACGGGTTTCCCCAg                  >  1:575893/1‑60 (MQ=255)
                                                         cgcgcgTCCGCCAATCCAGACGTTATTACCGATGGTGACGGGttt                        >  1:618554/1‑45 (MQ=255)
                                                         cgcgcgTCCGCCAATCCAGACGTTATTACCGATGGTGACGGGTTTCCCCAGTTCAGCACCGCTAt    >  1:728076/1‑65 (MQ=255)
                                                         cgcgcgTCCGCCAATCCAGACGTTATTACCGATGGTGACGGGTTTCCCCAGTTCAGCACCGCTAt    >  1:469654/1‑65 (MQ=255)
                                                         cgcgcgTCCGCCAATCCAGACGTTATTACCGATGGTGACGGGTTTCCCCAGTTCAGCACCGCTAt    >  1:422008/1‑65 (MQ=255)
                                                         cgcgcgTCCGCCAATCCAGACGTTATTACCGATGGTGACGGGTTTCCCCAGTTCAGCACCGCTAt    >  1:2971634/1‑65 (MQ=255)
                                                         cgcgcgTCCGCCAATCCAGACGTTATTACCGATGGTGACGGGTTTCCCCAGTTCAGCACCGCTAt    >  1:1689514/1‑65 (MQ=255)
                                                         cgcgcgTCCGCCAATCCAGACGTTATTACCGATGGTGACGGGTTTCCCCAGTTCAGCACCGCTAt    >  1:884659/1‑65 (MQ=255)
                                                         cgcgcgTCCGCCAATCCAGACGTTATTACCGATGGTGACGGGTTTCCCCAGTTCAGCACCGCTAt    >  1:2850221/1‑65 (MQ=255)
                                                         cgcgcgTCCGCCAATCCAGACGTTATTACCGATGGTGACGGGTTTCCCCAGTTCAGCACCGCTAt    >  1:3246102/1‑65 (MQ=255)
                                                         cgcgcgTCCGCCAATCCAGACGTTATTACCGATGGTGACGGGTTTCCCCAGTTCAGCACCGCTAt    >  1:519000/1‑65 (MQ=255)
                                                         agcgcgTCCGCCAATCCAGACGTTATTACCGATGGTg                                >  1:703166/2‑37 (MQ=255)
                                                            |                                                               
AACTGCACCTGAGGCTACCACGACGTTATCACCAATGGTCACACCAGGGTTAATGACCGCGCGTCCGCCAATCCAGACGTTATTACCGATGGTGACGGGTTTCCCCAGTTCAGCACCGCTATTA  >  minE/307905‑308028

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: